Q9HEM6 (GDA1_KLULA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-diphosphatase
Short name=GDPase
EC=3.6.1.42

Gene names

Name:	GDA1
Ordered Locus Names:	KLLA0F16863g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol. Ref.1
Catalytic activity	GDP + H2O = GMP + phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.1.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	guanosine-diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Guanosine-diphosphatase		PRO_0000209917
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 26	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	27 – 522	496	Lumenal Potential
Sites
	Active site	220	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HEM6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A7A27902607A6732	FASTA	522	56,863
	10 20 30 40 50 60 MHINSVVRNY RFLIGALTAL MLLLLLRSSA SPQVGSLANS NKVDTPDGLK VPGASSPISQ 70 80 90 100 110 120 NINEDSQKQT AKDHTSQNLE SGSNSLSKSQ CTEGHKYVVM IDAGSTGSRV HVYEFDVCTQ 130 140 150 160 170 180 PPTLINETFE MLKPGLSSFD IDAVGAAKSL DPLLKIAMDA VPKDKRNCTP VAVKATAGLR 190 200 210 220 230 240 MLGDEKSSKI LAQVRKHLEQ DYPFPVVDGD GVSIMDGEEE GVYAWVTANY LLGNIGAGSK 250 260 270 280 290 300 LPTAAVFDLG GGSTQIVFEP SFPPNEKMVD GEHKYELSFG GHDYTLYQFS HLGYGLMQGR 310 320 330 340 350 360 NKINTELVNV AISSGTITKG QTARTYELSS PCLPPGTTAE GEKVKISDDE IYTVNFKGPK 370 380 390 400 410 420 VPAGPQCRYL ADKILNKDAK CNTPPCSFNG IHQPSLVHTF KETSDLYVFS YFYDRTQPLG 430 440 450 460 470 480 LPLSFTLQEL QDLARTVCNG EEVWESVFSG IEGSLSELSK EPQWCLDLNF QVSLLHTGYD 490 500 510 520 IPLQRELRTA KTIANNELGW CLGASLPLLE SANWSCKVSK VE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The UDPase activity of the Kluyveromyces lactis Golgi GDPase has a role in uridine nucleotide sugar transport into Golgi vesicles." Lopez-Avalos M.D., Uccelletti D., Abeijon C., Hirschberg C.B. Glycobiology 11:413-422(2001) [PubMed: 11425802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 201343 / MG1/2.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ401304 Genomic DNA. Translation: CAC21576.1. CR382126 Genomic DNA. Translation: CAG98545.1.
RefSeq	XP_455837.1. XM_455837.1.
3D structure databases
ProteinModelPortal	Q9HEM6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9HEM6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2895656.
GenomeReviews	Gene locus KLLA0F16863g in contig CR382126_GR.
KEGG	kla:KLLA0F16863g.
Phylogenomic databases
eggNOG	fuNOG05233.
HOGENOM	HBG736908.
OMA	PPCSFNG.
OrthoDB	EOG447K2K.
Enzyme and pathway databases
BRENDA	3.6.1.42. 2825.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. IPR015605. Guanosine_diphosphatase. [Graphical view]
KO	K01526.
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit. PTHR11782:SF9. Gda1p. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GDA1_KLULA

Accession

Primary (citable) accession number: Q9HEM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program