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Protein

Autophagy-related protein 18

Gene

atg18

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Necessary for proper vacuole morphology. Plays an important role in osmotically-induced vacuole fragmentation. Required for cytoplasm to vacuole transport (Cvt) vesicle formation, pexophagy and starvation-induced autophagy. Involved in correct atg9 trafficking to the preautophagosomal structure. Might also be involved in premeiotic DNA replication (By similarity). Required for the recruitment of the atg5-atg12/atg16 complex to the preautophagosomal structure.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagy Source: PomBase
  • macroautophagy Source: PomBase
  • mitophagy Source: GO_Central
  • nucleophagy Source: GO_Central
  • protein lipidation Source: GO_Central
  • protein localization to pre-autophagosomal structure Source: GO_Central
  • protein targeting to vacuole Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-SPO-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 18
Gene namesi
Name:atg18
Synonyms:atg18a
ORF Names:SPAC589.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC589.07c.
PomBaseiSPAC589.07c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • endosome Source: PomBase
  • endosome membrane Source: UniProtKB-SubCell
  • extrinsic component of membrane Source: GO_Central
  • fungal-type vacuole membrane Source: PomBase
  • Golgi apparatus Source: PomBase
  • pre-autophagosomal structure Source: PomBase
  • pre-autophagosomal structure membrane Source: GO_Central
  • protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Impairs atg8-processing.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi228R → T: Impairs localization and pre-autophagosomal structure formation; when associated with Thr-229. 1 Publication1
Mutagenesisi229R → T: Impairs localization and pre-autophagosomal structure formation; when associated with Thr-228. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508721 – 373Autophagy-related protein 18Add BLAST373

Proteomic databases

MaxQBiQ9HDZ7.
PRIDEiQ9HDZ7.

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex (By similarity). Interacts with atg5.By similarity1 Publication

Protein-protein interaction databases

BioGridi278655. 52 interactors.
MINTiMINT-4702872.

Structurei

3D structure databases

ProteinModelPortaliQ9HDZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati144 – 183WD 1Add BLAST40
Repeati186 – 226WD 2Add BLAST41
Repeati231 – 270WD 3Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi227 – 231FRRGT-motif5

Domaini

The N-terminus might form a beta-propeller domain involved in specific binding to phosphatidylinositol 3,5-bisphosphate (PIP2), leading to the association of the protein to the membrane.By similarity
The FRRGT-motif is essential for the cytoplasm to vacuole transport (Cvt) pathway, for the recruitment of atg8 and atg16 to the PAS in nutrient-rich medium, and for its recruitment to and dissociation from the PAS under starvation conditions.1 Publication

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated
Contains 3 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000217543.
InParanoidiQ9HDZ7.
KOiK17908.
OMAiIRRTSQN.
OrthoDBiEOG092C3UKJ.
PhylomeDBiQ9HDZ7.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HDZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFFVRKYRG KAALLSIGTF DGYKIYNCDP FGKCFHKIQG ATSIVEMLFS
60 70 80 90 100
TSLVALVEKD DGNNRKLKLI NTKKSTTICE LTFPTPLLAV KLNRKRLLAV
110 120 130 140 150
LEEQIYVYDI SNMLLLHTIE TTSNVFAVCA LSPNSENCYL AYPDSRDHEP
160 170 180 190 200
RTEGESSSPN VSNSAVSGQV ILWDVINCKQ ITKIEAHKDS LACLAFNSDG
210 220 230 240 250
TMLATASDNG RIIRVFAIPS GQRLYQFRRG SLPAQIYSIA FHPDSSLLTV
260 270 280 290 300
TSSTQTVHIF RLKEVYSNLE RQGLLPSSPP PKESLLRRSS RSLIGTVGGY
310 320 330 340 350
LPQSVSGMLD PERDFAYAHI PGDKVTSIAA FGPDNTIVNV ATYDGNLYSF
360 370
RVNLRTGGEC AMVNHFCVGL TAA
Length:373
Mass (Da):41,109
Last modified:March 1, 2001 - v1
Checksum:iB7609062573AF159
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAC19764.1.
RefSeqiNP_594055.1. NM_001019479.2.

Genome annotation databases

EnsemblFungiiSPAC589.07c.1; SPAC589.07c.1:pep; SPAC589.07c.
GeneIDi2542180.
KEGGispo:SPAC589.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAC19764.1.
RefSeqiNP_594055.1. NM_001019479.2.

3D structure databases

ProteinModelPortaliQ9HDZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278655. 52 interactors.
MINTiMINT-4702872.

Proteomic databases

MaxQBiQ9HDZ7.
PRIDEiQ9HDZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC589.07c.1; SPAC589.07c.1:pep; SPAC589.07c.
GeneIDi2542180.
KEGGispo:SPAC589.07c.

Organism-specific databases

EuPathDBiFungiDB:SPAC589.07c.
PomBaseiSPAC589.07c.

Phylogenomic databases

HOGENOMiHOG000217543.
InParanoidiQ9HDZ7.
KOiK17908.
OMAiIRRTSQN.
OrthoDBiEOG092C3UKJ.
PhylomeDBiQ9HDZ7.

Enzyme and pathway databases

ReactomeiR-SPO-1632852. Macroautophagy.

Miscellaneous databases

PROiQ9HDZ7.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATG18_SCHPO
AccessioniPrimary (citable) accession number: Q9HDZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.