Q9HDX8 (ALO_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-arabinono-1,4-lactone oxidase
Short name=ALO
EC=1.1.3.37

Alternative name(s):
L-galactono-gamma-lactone oxidase

Gene names

Name:	alo1
ORF Names:	SPAPB1A10.12c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-arabinono-1,4-lactone + O2 = dehydro-D-arabinono-1,4-lactone + H2O2.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; D-erythroascorbate biosynthesis; dehydro-D-arabinono-1,4-lactone from D-arabinose: step 2/2.
Subcellular location	Mitochondrion membrane By similarity. Note: Membrane-embedded By similarity.
Sequence similarities	Belongs to the oxygen-dependent FAD-linked oxidoreductase family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biosynthetic process Inferred from electronic annotation. Source: InterPro cellular response to oxidative stress Inferred from expression pattern PubMed 12529438. Source: PomBase
Cellular_component	integral to mitochondrial outer membrane Inferred from sequence orthology. Source: PomBase
Molecular_function	D-arabinono-1,4-lactone oxidase activity Inferred from sequence orthology. Source: PomBase UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	D-arabinono-1,4-lactone oxidase		PRO_0000128169
Regions
	Domain	24 – 194	171	FAD-binding PCMH-type
Amino acid modifications
	Modified residue	61	1	Pros-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HDX8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A3BE6D8D08E2F273	FASTA	461	52,028
	10 20 30 40 50 60 MSIPHINKLS QDGRVRFSNW AKTFSAISLG LRCPKTEEQL REILVDANSN GKKIRVVGAG 70 80 90 100 110 120 HSPSDIVCTS GYLLSLDKMN KVVSFDPDSL SITVQAGIRF YQVQEILQNL GYSLPIVGSI 130 140 150 160 170 180 SETSVSGIMS TCTHGSSLQH QVLPHYIKSM RIMLADGSIV TCSRELQKDM FAAAQVSLGA 190 200 210 220 230 240 LGVIVDITIS VVPAFDLVAT EDVTTVTDLF QDWKNNLIWE SAEFVRVHVF PYANRAVVWR 250 260 270 280 290 300 ANKVEPNTVP HTPKPSLFRL KLDSFVYQCL LFVGKCVNRV TPYLERFWFK CHYGSKLGTA 310 320 330 340 350 360 LQVAGPGFDV LQMFCYFSQH VSEWGIPLES APDALEKLIN YTVDDAGKIG AYTHWPIEVR 370 380 390 400 410 420 VCAPTPEDEC WLSTDCKVPT CYIEAIMYRP FSTSINYKPY FKALEDIANQ YNGKPHWAKE 430 440 450 460 YSLTKEQLLE RYPNLSKWLS LRKLLDPKGV FWNDYLQRHL G

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAC21485.1.
RefSeq	NP_593526.1. NM_001018960.2.
3D structure databases
ProteinModelPortal	Q9HDX8.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4702705.
STRING	4896.SPAPB1A10.12c-1.
Proteomic databases
PaxDb	Q9HDX8.
PRIDE	Q9HDX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAPB1A10.12c.1; SPAPB1A10.12c.1:pep; SPAPB1A10.12c.
GeneID	2543430.
KEGG	spo:SPAPB1A10.12c.
Organism-specific databases
PomBase	SPAPB1A10.12c.
Phylogenomic databases
eggNOG	COG0277.
HOGENOM	HOG000204635.
OMA	HRHFGFF.
OrthoDB	EOG4GF6PD.
Enzyme and pathway databases
UniPathway	UPA00771; UER00766.
Family and domain databases
Gene3D	3.30.43.10. 1 hit. 3.30.465.10. 1 hit.
InterPro	IPR007173. ALO. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR010031. FAD_lactone_oxidase. IPR023595. LGO_GLO. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Pfam	PF04030. ALO. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
PIRSF	PIRSF000136. LGO_GLO. 1 hit.
SUPFAM	SSF56176. FAD-binding_2. 1 hit.
TIGRFAMs	TIGR01678. FAD_lactone_ox. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. PS00862. OX2_COVAL_FAD. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804443.

Entry information

Entry name

ALO_SCHPO

Accession

Primary (citable) accession number: Q9HDX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program