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Protein

Enolase

Gene

ENO

Organism
Alternaria alternata (Alternaria rot fungus) (Torula alternata)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591SubstrateBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Active sitei211 – 2111Proton donorBy similarity
Metal bindingi246 – 2461MagnesiumBy similarity
Metal bindingi297 – 2971MagnesiumBy similarity
Binding sitei297 – 2971SubstrateBy similarity
Metal bindingi322 – 3221MagnesiumBy similarity
Binding sitei322 – 3221SubstrateBy similarity
Active sitei347 – 3471Proton acceptorBy similarity
Binding sitei398 – 3981SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Allergen Alt a 11
Allergen Alt a 5
Allergen Alt a XI
Allergen: Alt a 6
Gene namesi
Name:ENO
Synonyms:ALTA11, ALTA6
OrganismiAlternaria alternata (Alternaria rot fungus) (Torula alternata)
Taxonomic identifieri5599 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeAlternariaAlternaria alternata group

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.2 Publications

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei14. Alt a 6.
3063. Alt a 6.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438EnolasePRO_0000134039Add
BLAST

Proteomic databases

PRIDEiQ9HDT3.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9HDT3.
SMRiQ9HDT3. Positions 3-432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni374 – 3774Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the enolase family.Curated

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HDT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTITKIHARS VYDSRGNPTV EVDIVTETGL HRAIVPSGAS TGSHEACELR
60 70 80 90 100
DGDKSKWGGK GVTKAVANVN DTIAPALIKE KLDVKDQSAV DAFLNKLDGT
110 120 130 140 150
TNKTNLGANA ILGVSMAIAK AAAAEKGVPL YAHISDLAGT KKPYVLPVPF
160 170 180 190 200
QNVLNGGSHA GGRLAFQEFM IVPCEAPTFS EAMRQGAEVY QKLKALAKKT
210 220 230 240 250
YGQSAGNVGD EGGVAPDIQT AEEALDLITK AIEEAGYTGK IKIAMDVASS
260 270 280 290 300
EFYKADEKKY DLDFKNPDSD KSKWLTYEQL AEMYKSLAEK YPIVSIEDPF
310 320 330 340 350
AEDDWEAWSY FFKTYDGQIV GDDLTVTNPE FIKKAIELKS CNALLLKVNQ
360 370 380 390 400
IGTITEAIQA AKDAFGAGWG VMVSHRSGET EDVTIADIVV GLRSGQIKTG
410 420 430
APARSERLAK LNQILRIEEE LGDNAVYAGN NFRTAVNL
Length:438
Mass (Da):47,205
Last modified:June 20, 2001 - v2
Checksum:iD60900100A72AF83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82437 mRNA. Translation: AAG42022.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82437 mRNA. Translation: AAG42022.2.

3D structure databases

ProteinModelPortaliQ9HDT3.
SMRiQ9HDT3. Positions 3-432.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei14. Alt a 6.
3063. Alt a 6.0101.

Proteomic databases

PRIDEiQ9HDT3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALLERGEN.
    Strain: 08-0203-Berlin.
  2. "Enolases are highly conserved fungal allergens."
    Breitenbach M., Simon B., Probst G., Oberkofler H., Ferreira F., Briza P., Achatz G., Unger A., Ebner C., Kraft D., Hirschwehr R.
    Int. Arch. Allergy Immunol. 113:114-117(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALLERGEN.
  3. "The allergens of Cladosporium herbarum and Alternaria alternata."
    Breitenbach M., Simon-Nobbe B.
    Chem. Immunol. 81:48-72(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  4. Cited for: REVIEW.

Entry informationi

Entry nameiENO_ALTAL
AccessioniPrimary (citable) accession number: Q9HDT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: May 11, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.