Q9HDG2 (NCPR_PHACH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH--cytochrome P450 reductase
Short name=CPR
Short name=P450R
EC=1.6.2.4

Gene names

Name:

CPR

Organism

Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)

Taxonomic identifier

5306 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetes incertae sedis › Corticiales › Corticiaceae › Phanerochaete

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. UniProtKB P16435
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD By similarity. FMN By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	NADPH--cytochrome P450 reductase		PRO_0000167607
Regions
	Transmembrane	8 – 24	17	Helical; Potential
	Domain	66 – 216	151	Flavodoxin-like
	Domain	269 – 546	278	FAD-binding FR-type
	Nucleotide binding	162 – 193	32	FMN By similarity UniProtKB P16435
	Nucleotide binding	310 – 321	12	FAD By similarity UniProtKB P16435
	Nucleotide binding	452 – 463	12	FAD By similarity UniProtKB P16435
	Nucleotide binding	572 – 590	19	NADP By similarity UniProtKB P16435
	Nucleotide binding	682 – 698	17	NADP By similarity UniProtKB P16435

Sequences

	Sequence		Length	Mass (Da)
	Q9HDG2 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: AC45F12C96198AB1	FASTA	736	81,628
	10 20 30 40 50 60 MAVSSSSDVI VLSVGIILAA LYLFREQIFS AAKPKTVQVP SSKAAAGGNG NPRDFIAKMK 70 80 90 100 110 120 EGKKRIVIFY GSQTGTAEEY AIRLAKEAKS KFGLASLVCD PEEYDFENLD QVPEDCCVFF 130 140 150 160 170 180 VMATYGEGEP TDNAVQLCQN LSDESFEFSN GEHKLPGLKY VIFGLGNKTY EHYNLISRNV 190 200 210 220 230 240 DRDLQKMGAI RIGERGEGDD DKSMEEDYLE WKDGMWEAFA KAMNVEEGQG GDSPDFVVTE 250 260 270 280 290 300 VFDHPEEKVY LGELSARALT RTKGIHDAKN PYPAPIIAAK ELFAPGSDRN CVHIELSTES 310 320 330 340 350 360 SGITYQHGDH VGVWPSNADK EVDRLLYALG LHEKKDTVIN IESLDPALAK VPFPVPTTYA 370 380 390 400 410 420 TVLRHYIDIS ALAGRQILGV LAKFAPNPEA EAVLKDLNSN KEHYQNIVAN GCMKLGEVLQ 430 440 450 460 470 480 YAAGNDLHAD PTASNTTAWK IPFDIIVSSI PRLQPRYYSI SSSPKLYPNA IHATVVVLKY 490 500 510 520 530 540 KSEKAPRVEE RWIYGVGSNF LLNLKYASHH DKAATLVSDD SPSEPSIVSH YPTYSIEGPR 550 560 570 580 590 600 GAYKQGDVVK VPIHVRRSTF RLPTNPKSPV IMIGPGTGVA PFRGFVQERV AMARRTIEKH 610 620 630 640 650 660 GPEGLADWGP IRLYYGCRRS DQDFLYKDEW PEYAKELHGK FIMRCAFSRE PPYKPDGSKI 670 680 690 700 710 720 YVQDLIWEDA EQIADAILNG KGYVYICGDA KSMSKSVEET LCRILGEAKG GSAEVEGAAE 730 LKLLKERNRL LLDVWS

References

[1]	"Cytochrome P450 oxidoreductase gene and its differentially terminated cDNAs from the white rot fungus Phanerochaete chrysosporium." Yadav J.S., Loper J.C. Curr. Genet. 37:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: ATCC 24725 / DSM 6909 / CBS 481.73 / BCRC 36200 / NRRL 6361 / VKM F-1767.
[2]	"Phanerochaete chrysosporium NADPH-cytochrome P450 reductase kinetic mechanism." Warrilow A.G.S., Lamb D.C., Kelly D.E., Kelly S.L. Biochem. Biophys. Res. Commun. 299:189-195(2002) [PubMed] [Europe PMC] [Abstract] Cited for: KINETIC MECHANISM.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF193060 Genomic DNA. Translation: AAG31349.1. AF193061 mRNA. Translation: AAG31350.1. AF193062 mRNA. Translation: AAG31351.1.
3D structure databases
ProteinModelPortal	Q9HDG2.
ModBase	Search...
Protein-protein interaction databases
STRING	5306.JGI74736.
Proteomic databases
PRIDE	Q9HDG2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0369.
Family and domain databases
Gene3D	1.20.990.10. 1 hit.
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR001433. OxRdtase_FAD/NAD-bd. IPR023208. P450R. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF000208. P450R. 1 hit.
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCPR_PHACH

Accession

Primary (citable) accession number: Q9HDG2
Secondary accession number(s): Q9HG14

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program