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Q9HDG2

- NCPR_PHACH

UniProt

Q9HDG2 - NCPR_PHACH

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Protein
NADPH--cytochrome P450 reductase
Gene
CPR
Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.By similarity

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

FAD By similarity.
FMN By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 19332FMN By similarityBy similarity
Add
BLAST
Nucleotide bindingi310 – 32112FAD By similarityBy similarity
Add
BLAST
Nucleotide bindingi452 – 46312FAD By similarityBy similarity
Add
BLAST
Nucleotide bindingi572 – 59019NADP By similarityBy similarity
Add
BLAST
Nucleotide bindingi682 – 69817NADP By similarityBy similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:CPR
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesCorticialesCorticiaceaePhanerochaete

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2417Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736NADPH--cytochrome P450 reductase
PRO_0000167607Add
BLAST

Proteomic databases

PRIDEiQ9HDG2.

Interactioni

Protein-protein interaction databases

STRINGi5306.JGI74736.

Structurei

3D structure databases

ProteinModelPortaliQ9HDG2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 216151Flavodoxin-like
Add
BLAST
Domaini269 – 546278FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0369.
OMAiLWQLIHE.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HDG2-1 [UniParc]FASTAAdd to Basket

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MAVSSSSDVI VLSVGIILAA LYLFREQIFS AAKPKTVQVP SSKAAAGGNG    50
NPRDFIAKMK EGKKRIVIFY GSQTGTAEEY AIRLAKEAKS KFGLASLVCD 100
PEEYDFENLD QVPEDCCVFF VMATYGEGEP TDNAVQLCQN LSDESFEFSN 150
GEHKLPGLKY VIFGLGNKTY EHYNLISRNV DRDLQKMGAI RIGERGEGDD 200
DKSMEEDYLE WKDGMWEAFA KAMNVEEGQG GDSPDFVVTE VFDHPEEKVY 250
LGELSARALT RTKGIHDAKN PYPAPIIAAK ELFAPGSDRN CVHIELSTES 300
SGITYQHGDH VGVWPSNADK EVDRLLYALG LHEKKDTVIN IESLDPALAK 350
VPFPVPTTYA TVLRHYIDIS ALAGRQILGV LAKFAPNPEA EAVLKDLNSN 400
KEHYQNIVAN GCMKLGEVLQ YAAGNDLHAD PTASNTTAWK IPFDIIVSSI 450
PRLQPRYYSI SSSPKLYPNA IHATVVVLKY KSEKAPRVEE RWIYGVGSNF 500
LLNLKYASHH DKAATLVSDD SPSEPSIVSH YPTYSIEGPR GAYKQGDVVK 550
VPIHVRRSTF RLPTNPKSPV IMIGPGTGVA PFRGFVQERV AMARRTIEKH 600
GPEGLADWGP IRLYYGCRRS DQDFLYKDEW PEYAKELHGK FIMRCAFSRE 650
PPYKPDGSKI YVQDLIWEDA EQIADAILNG KGYVYICGDA KSMSKSVEET 700
LCRILGEAKG GSAEVEGAAE LKLLKERNRL LLDVWS 736
Length:736
Mass (Da):81,628
Last modified:March 1, 2001 - v1
Checksum:iAC45F12C96198AB1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF193060 Genomic DNA. Translation: AAG31349.1.
AF193061 mRNA. Translation: AAG31350.1.
AF193062 mRNA. Translation: AAG31351.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF193060 Genomic DNA. Translation: AAG31349.1 .
AF193061 mRNA. Translation: AAG31350.1 .
AF193062 mRNA. Translation: AAG31351.1 .

3D structure databases

ProteinModelPortali Q9HDG2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5306.JGI74736.

Proteomic databases

PRIDEi Q9HDG2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0369.
OMAi LWQLIHE.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cytochrome P450 oxidoreductase gene and its differentially terminated cDNAs from the white rot fungus Phanerochaete chrysosporium."
    Yadav J.S., Loper J.C.
    Curr. Genet. 37:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: ATCC 24725 / DSM 6909 / CBS 481.73 / BCRC 36200 / NRRL 6361 / VKM F-1767.
  2. "Phanerochaete chrysosporium NADPH-cytochrome P450 reductase kinetic mechanism."
    Warrilow A.G.S., Lamb D.C., Kelly D.E., Kelly S.L.
    Biochem. Biophys. Res. Commun. 299:189-195(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: KINETIC MECHANISM.

Entry informationi

Entry nameiNCPR_PHACH
AccessioniPrimary (citable) accession number: Q9HDG2
Secondary accession number(s): Q9HG14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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