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Q9HDG2

- NCPR_PHACH

UniProt

Q9HDG2 - NCPR_PHACH

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Protein

NADPH--cytochrome P450 reductase

Gene

CPR

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.By similarity

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

FAD.By similarity
FMN.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 19332FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi310 – 32112FADBy similarityAdd
BLAST
Nucleotide bindingi452 – 46312FADBy similarityAdd
BLAST
Nucleotide bindingi572 – 59019NADPBy similarityAdd
BLAST
Nucleotide bindingi682 – 69817NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:CPR
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)Imported
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesCorticialesCorticiaceaePhanerochaete

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736NADPH--cytochrome P450 reductasePRO_0000167607Add
BLAST

Proteomic databases

PRIDEiQ9HDG2.

Interactioni

Protein-protein interaction databases

STRINGi5306.JGI74736.

Structurei

3D structure databases

ProteinModelPortaliQ9HDG2.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2417HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 216151Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini269 – 546278FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0369.
OMAiLWQLIHE.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HDG2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVSSSSDVI VLSVGIILAA LYLFREQIFS AAKPKTVQVP SSKAAAGGNG
60 70 80 90 100
NPRDFIAKMK EGKKRIVIFY GSQTGTAEEY AIRLAKEAKS KFGLASLVCD
110 120 130 140 150
PEEYDFENLD QVPEDCCVFF VMATYGEGEP TDNAVQLCQN LSDESFEFSN
160 170 180 190 200
GEHKLPGLKY VIFGLGNKTY EHYNLISRNV DRDLQKMGAI RIGERGEGDD
210 220 230 240 250
DKSMEEDYLE WKDGMWEAFA KAMNVEEGQG GDSPDFVVTE VFDHPEEKVY
260 270 280 290 300
LGELSARALT RTKGIHDAKN PYPAPIIAAK ELFAPGSDRN CVHIELSTES
310 320 330 340 350
SGITYQHGDH VGVWPSNADK EVDRLLYALG LHEKKDTVIN IESLDPALAK
360 370 380 390 400
VPFPVPTTYA TVLRHYIDIS ALAGRQILGV LAKFAPNPEA EAVLKDLNSN
410 420 430 440 450
KEHYQNIVAN GCMKLGEVLQ YAAGNDLHAD PTASNTTAWK IPFDIIVSSI
460 470 480 490 500
PRLQPRYYSI SSSPKLYPNA IHATVVVLKY KSEKAPRVEE RWIYGVGSNF
510 520 530 540 550
LLNLKYASHH DKAATLVSDD SPSEPSIVSH YPTYSIEGPR GAYKQGDVVK
560 570 580 590 600
VPIHVRRSTF RLPTNPKSPV IMIGPGTGVA PFRGFVQERV AMARRTIEKH
610 620 630 640 650
GPEGLADWGP IRLYYGCRRS DQDFLYKDEW PEYAKELHGK FIMRCAFSRE
660 670 680 690 700
PPYKPDGSKI YVQDLIWEDA EQIADAILNG KGYVYICGDA KSMSKSVEET
710 720 730
LCRILGEAKG GSAEVEGAAE LKLLKERNRL LLDVWS
Length:736
Mass (Da):81,628
Last modified:March 1, 2001 - v1
Checksum:iAC45F12C96198AB1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF193060 Genomic DNA. Translation: AAG31349.1.
AF193061 mRNA. Translation: AAG31350.1.
AF193062 mRNA. Translation: AAG31351.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF193060 Genomic DNA. Translation: AAG31349.1 .
AF193061 mRNA. Translation: AAG31350.1 .
AF193062 mRNA. Translation: AAG31351.1 .

3D structure databases

ProteinModelPortali Q9HDG2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5306.JGI74736.

Proteomic databases

PRIDEi Q9HDG2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0369.
OMAi LWQLIHE.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cytochrome P450 oxidoreductase gene and its differentially terminated cDNAs from the white rot fungus Phanerochaete chrysosporium."
    Yadav J.S., Loper J.C.
    Curr. Genet. 37:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: ATCC 24725 / DSM 6909 / CBS 481.73 / BCRC 36200 / NRRL 6361 / VKM F-1767.
  2. "Phanerochaete chrysosporium NADPH-cytochrome P450 reductase kinetic mechanism."
    Warrilow A.G.S., Lamb D.C., Kelly D.E., Kelly S.L.
    Biochem. Biophys. Res. Commun. 299:189-195(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: KINETIC MECHANISM.

Entry informationi

Entry nameiNCPR_PHACH
AccessioniPrimary (citable) accession number: Q9HDG2
Secondary accession number(s): Q9HG14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3