Reviewed, UniProtKB/Swiss-Prot Q9HDC9 (APMAP_HUMAN)

Names and origin

Protein names

Recommended name:
    Adipocyte plasma membrane-associated protein
Alternative name(s):
    Protein BSCv

Gene names

Name:	APMAP
Synonyms:	C20orf3
ORF Names:	UNQ1869/PRO4305

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation. Ref.8
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Liver, glomerular and tubular structures of the kidney, endothelial cells, arterial wall and pancreatic islets of Langerhans (at protein level). Found ubiquitously in adult as well as in embryonic tissues. In adult tissue, the highest expression is found in the liver, placenta and heart. Found on the cell surface of monocytes. In embryonic tissue, the highest expression levels is found in the liver and the kidney. Ref.8
Sequence similarities	Belongs to the strictosidine synthase family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell surface Ref.8 Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylesterase activity Ref.8 Inferred from direct assay. Source: UniProtKB strictosidine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9HDC9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9HDC9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     283-289: RVYVSGL → SSLVKRR
     290-416: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Adipocyte plasma membrane-associated protein		PRO_0000205945
Regions
	Topological domain	1 – 40	40	Cytoplasmic Potential
	Transmembrane	41 – 61	21	Signal-anchor for type II membrane protein Potential
	Topological domain	62 – 416	355	Extracellular Potential
Amino acid modifications
	Glycosylation	160	1	N-linked (GlcNAc...) Ref.6 Ref.7 Ref.10
	Glycosylation	196	1	N-linked (GlcNAc...) Ref.6 Ref.7 Ref.10
Natural variations
	Alternative sequence	283 – 289	7	RVYVSGL → SSLVKRR in isoform 2.		VSP_036992
	Alternative sequence	290 – 416	127	Missing in isoform 2.		VSP_036993
	Natural variant	65	1	I → V: dbSNP rs17298715.		VAR_014128
	Natural variant	282	1	R → Q: dbSNP rs35097515. Ref.8		VAR_055039
	Natural variant	374	1	R → W: dbSNP rs28364786. Ref.8		VAR_055040
Experimental info
	Sequence conflict	12	1	P → A in BAG63373. Ref.2
	Sequence conflict	74	1	E → G in BAF83818. Ref.2
	Sequence conflict	295	1	D → G in BAF83818. Ref.2
	Sequence conflict	416	1	V → A in BAF83818. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 19, 2002. Version 2. Checksum: C88E2DF2DF6093C4	FASTA	416	46,480
	10 20 30 40 50 60 MSEADGLRQR RPLRPQVVTD DDGQAPEAKD GSSFSGRVFR VTFLMLAVSL TVPLLGAMML 70 80 90 100 110 120 LESPIDPQPL SFKEPPLLLG VLHPNTKLRQ AERLFENQLV GPESIAHIGD VMFTGTADGR 130 140 150 160 170 180 VVKLENGEIE TIARFGSGPC KTRDDEPVCG RPLGIRAGPN GTLFVADAYK GLFEVNPWKR 190 200 210 220 230 240 EVKLLLSSET PIEGKNMSFV NDLTVTQDGR KIYFTDSSSK WQRRDYLLLV MEGTDDGRLL 250 260 270 280 290 300 EYDTVTREVK VLLDQLRFPN GVQLSPAEDF VLVAETTMAR IRRVYVSGLM KGGADLFVEN 310 320 330 340 350 360 MPGFPDNIRP SSSGGYWVGM STIRPNPGFS MLDFLSERPW IKRMIFKLFS QETVMKFVPR 370 380 390 400 410 YSLVLELSDS GAFRRSLHDP DGLVATYISE VHEHDGHLYL GSFRSPFLCR LSLQAV
	Isoform 2. Checksum: 548C7BAA8D6FE9F2	FASTA	289	32,161
	10 20 30 40 50 60 MSEADGLRQR RPLRPQVVTD DDGQAPEAKD GSSFSGRVFR VTFLMLAVSL TVPLLGAMML 70 80 90 100 110 120 LESPIDPQPL SFKEPPLLLG VLHPNTKLRQ AERLFENQLV GPESIAHIGD VMFTGTADGR 130 140 150 160 170 180 VVKLENGEIE TIARFGSGPC KTRDDEPVCG RPLGIRAGPN GTLFVADAYK GLFEVNPWKR 190 200 210 220 230 240 EVKLLLSSET PIEGKNMSFV NDLTVTQDGR KIYFTDSSSK WQRRDYLLLV MEGTDDGRLL 250 260 270 280 EYDTVTREVK VLLDQLRFPN GVQLSPAEDF VLVAETTMAR IRSSLVKRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3)." Morita M., Hara Y., Tamai Y., Arakawa H., Nishimura S. Genomics 67:87-91(2000) [PubMed: 10945474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Adipose tissue and Testis.
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
[5]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 39-416 (ISOFORM 1).
[6]	"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003) [PubMed: 12754519] [Abstract] Cited for: GLYCOSYLATION AT ASN-160 AND ASN-196.
[7]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-160 AND ASN-196, MASS SPECTROMETRY. Tissue: Plasma.
[8]	"Localization and characterization of the novel protein encoded by C20orf3." Ilhan A., Gartner W., Nabokikh A., Daneva T., Majdic O., Cohen G., Boehmig G.A., Base W., Hoerl W.H., Wagner L. Biochem. J. 414:485-495(2008) [PubMed: 18513186] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANTS GLN-282 AND TRP-374.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-160 AND ASN-196, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB033767 mRNA. Translation: BAB11885.1. Different initiation. AK291129 mRNA. Translation: BAF83818.1. AK301959 mRNA. Translation: BAG63373.1. AL035661 Genomic DNA. Translation: CAB75499.1. BC003501 mRNA. Translation: AAH03501.1. AY359076 mRNA. Translation: AAQ89435.1. Different initiation. AK025834 mRNA. Translation: BAB15253.1. Different initiation. AK026866 mRNA. Translation: BAB15578.1. Different initiation.
IPI	IPI00031131. IPI00929530.
RefSeq	NP_065392.1.
UniGene	Hs.472330
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HDC9. 2 interactions.
STRING	Q9HDC9.
PTM databases
PhosphoSite	Q9HDC9.
Proteomic databases
PRIDE	Q9HDC9.
Genome annotation databases
Ensembl	ENST00000217456; ENSP00000217456; ENSG00000101474; Homo sapiens. [Genome view]
GeneID	57136.
KEGG	hsa:57136.
UCSC	uc002wty.1. human.
Organism-specific databases
CTD	57136.
GeneCards	GC20M024891.
H-InvDB	HIX0015698.
HGNC	HGNC:13238. C20orf3.
HPA	HPA012863.
PharmGKB	PA25745.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9HDC9.
OMA	LFEVNPW
Gene expression databases
ArrayExpress	Q9HDC9.
Bgee	Q9HDC9.
CleanEx	HS_C20orf3.
Genevestigator	Q9HDC9.
GermOnline	ENSG00000101474. Homo sapiens.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR018119. Strictosidine_synth_cons-reg. IPR004141. Strictosidine_synthase. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
PANTHER	PTHR10426. Str_synth. 1 hit.
Pfam	PF03088. Str_synth. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	63063.

Entry information

Entry name

APMAP_HUMAN

Accession

Primary (citable) accession number: Q9HDC9
Secondary accession number(s): A8K514 , B4DXG1, Q6UVZ8, Q9GZS8, Q9NUB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	November 24, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.