ID   XAGE1_HUMAN             Reviewed;          81 AA.
AC   Q9HD64; A6NJ94; Q5JPN8; Q5JPP0; Q5JPP3; Q8WWG5; Q8WWG6; Q969J6;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 3.
DT   16-SEP-2015, entry version 123.
DE   RecName: Full=X antigen family member 1;
DE            Short=XAGE-1;
DE   AltName: Full=Cancer/testis antigen 12.1;
DE            Short=CT12.1;
DE   AltName: Full=G antigen family D member 2;
GN   Name=XAGE1A; Synonyms=GAGED2, XAGE1;
GN   and
GN   Name=XAGE1B;
GN   and
GN   Name=XAGE1C;
GN   and
GN   Name=XAGE1D;
GN   and
GN   Name=XAGE1E;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RC   TISSUE=Testis;
RX   PubMed=10987281;
RA   Liu X.F., Helman L.J., Yeung C., Bera T.K., Lee B., Pastan I.;
RT   "XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma.";
RL   Cancer Res. 60:4752-4755(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS B AND D).
RX   PubMed=11992404; DOI=10.1002/ijc.10371;
RA   Zendman A.J.W., van Kraats A.A., Weidle U.H., Ruiter D.J.,
RA   van Muijen G.N.P.;
RT   "The XAGE family of cancer/testis-associated genes: alignment and
RT   expression profile in normal tissues, melanoma lesions and Ewing's
RT   sarcoma.";
RL   Int. J. Cancer 99:361-369(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15772651; DOI=10.1038/nature03440;
RA   Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA   Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A.,
RA   Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G.,
RA   Jones M.C., Hurles M.E., Andrews T.D., Scott C.E., Searle S.,
RA   Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R.,
RA   Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L.,
RA   Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A.,
RA   Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S.,
RA   Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R.,
RA   Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M.,
RA   Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N.,
RA   Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D.,
RA   Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W.,
RA   Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C.,
RA   Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C.,
RA   Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA   Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA   Corby N., Connor R.E., David R., Davies J., Davis C., Davis J.,
RA   Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S.,
RA   Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I.,
RA   Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L.,
RA   Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P.,
RA   Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S.,
RA   Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A.,
RA   Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J.,
RA   Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J.,
RA   Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S.,
RA   de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z.,
RA   Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C.,
RA   Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W.,
RA   Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA   Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA   McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T.,
RA   Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I.,
RA   Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N.,
RA   Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J.,
RA   Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E.,
RA   Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S.,
RA   Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA   Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA   Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA   Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T.,
RA   Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S.,
RA   Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L.,
RA   Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A.,
RA   Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L.,
RA   Williams G., Williams L., Williamson A., Williamson H., Wilming L.,
RA   Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H.,
RA   Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A.,
RA   Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA   Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A.,
RA   Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T.,
RA   Gibbs R.A., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence of the human X chromosome.";
RL   Nature 434:325-337(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   TISSUE SPECIFICITY, AND IDENTIFICATION OF INITIATOR METHIONINE
RP   (ISOFORM B).
RX   PubMed=12479262;
RA   Egland K.A., Kumar V., Duray P., Pastan I.;
RT   "Characterization of overlapping XAGE-1 transcripts encoding a cancer
RT   testis antigen expressed in lung, breast, and other types of
RT   cancers.";
RL   Mol. Cancer Ther. 1:441-450(2002).
RN   [7]
RP   TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORMS B AND D), AND
RP   IDENTIFICATION OF INITIATOR METHIONINE (ISOFORM B).
RX   PubMed=17335148;
RA   Sato S., Noguchi Y., Ohara N., Uenaka A., Shimono M., Nakagawa K.,
RA   Koizumi F., Ishida T., Yoshino T., Shiratori Y., Nakayama E.;
RT   "Identification of XAGE-1 isoforms: predominant expression of XAGE-1b
RT   in testis and tumors.";
RL   Cancer Immun. 7:5-5(2007).
CC   -!- INTERACTION:
CC       Q8N9N5:BANP; NbExp=3; IntAct=EBI-2340004, EBI-744695;
CC       Q9Y6X0:SETBP1; NbExp=3; IntAct=EBI-2340004, EBI-2548259;
CC       Q8IY57:YAF2; NbExp=3; IntAct=EBI-2340004, EBI-2842031;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=B; Synonyms=XAGE-1a, XAGE-1b;
CC         IsoId=Q9HD64-2; Sequence=Displayed;
CC         Note=XAGE-1a and XAGE-1b mRNAs are produced by alternative
CC         promoter usage. However, for both isoforms, the translation
CC         initiator codon remains the same, generating an identical
CC         protein. XAGE-1b is the predominant transcript, compared to
CC         XAGE-1a.;
CC       Name=D; Synonyms=XAGE-1d;
CC         IsoId=Q9HD64-4; Sequence=VSP_001596;
CC   -!- TISSUE SPECIFICITY: In normal tissues, highly expressed in testis.
CC       Expressed also in many different types of cancers: highly
CC       expressed in breast cancer, prostate cancer and many types of lung
CC       cancers, including squamous cell carcinoma, small cell carcinoma,
CC       non-small cell carcinoma, and adenocarcinoma, as well as in
CC       Ewing's cell lines, in some Ewing's sarcoma patient samples, and
CC       in one of one alveolar rhabdomyosarcoma patient sample.
CC       {ECO:0000269|PubMed:12479262, ECO:0000269|PubMed:17335148}.
CC   -!- MISCELLANEOUS: According to PubMed:11992404, the transcription of
CC       XAGE1A is regulated by methylation of the CpG island in the
CC       promoter, and four alternative RNA splicing variants, XAGE-1a, b,
CC       c have been identified.
CC   -!- SIMILARITY: Belongs to the GAGE family. {ECO:0000305}.
CC   -!- CAUTION: PubMed:17335148, examines the translation products of
CC       theses four XAGE1A transcripts, and finds that the XAGE-1c
CC       transcript is possibly translated to 9- and 17-aa polypeptides and
CC       not to a protein consisting of 160 amino acids as shown in
CC       PubMed:11992404. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG01401.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
CC       Sequence=AAH09538.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
CC       Sequence=CAC82986.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
CC       Sequence=CAI40888.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAI40895.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAI41526.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAI41531.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAI41534.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AF251237; AAG01401.1; ALT_INIT; mRNA.
DR   EMBL; AJ290447; CAC38107.1; -; mRNA.
DR   EMBL; AJ400997; CAC38108.1; -; Genomic_DNA.
DR   EMBL; AJ318878; CAC82986.1; ALT_INIT; mRNA.
DR   EMBL; AJ318879; CAC82987.1; -; mRNA.
DR   EMBL; BT007099; AAP35763.1; -; mRNA.
DR   EMBL; AL772246; CAI41526.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL772246; CAI41527.1; -; Genomic_DNA.
DR   EMBL; AL772246; CAI41528.1; -; Genomic_DNA.
DR   EMBL; AL772246; CAI41530.1; -; Genomic_DNA.
DR   EMBL; AL772246; CAI41531.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL772246; CAI41532.1; -; Genomic_DNA.
DR   EMBL; AL772246; CAI41533.1; -; Genomic_DNA.
DR   EMBL; AL772246; CAI41534.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BX088602; CAI40888.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BX088602; CAI40889.1; -; Genomic_DNA.
DR   EMBL; BX088602; CAI40890.1; -; Genomic_DNA.
DR   EMBL; BX088602; CAI40893.1; -; Genomic_DNA.
DR   EMBL; BX088602; CAI40894.1; -; Genomic_DNA.
DR   EMBL; BX088602; CAI40895.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BC009538; AAH09538.2; ALT_INIT; mRNA.
DR   CCDS; CCDS48126.2; -. [Q9HD64-2]
DR   CCDS; CCDS48128.1; -. [Q9HD64-4]
DR   CCDS; CCDS75982.1; -. [Q9HD64-2]
DR   CCDS; CCDS75983.1; -. [Q9HD64-4]
DR   RefSeq; NP_001091063.2; NM_001097594.2. [Q9HD64-2]
DR   RefSeq; NP_001091065.1; NM_001097596.2. [Q9HD64-4]
DR   RefSeq; NP_001091073.2; NM_001097604.2. [Q9HD64-2]
DR   RefSeq; NP_001091074.1; NM_001097605.2. [Q9HD64-4]
DR   UniGene; Hs.112208; -.
DR   UniGene; Hs.546096; -.
DR   UniGene; Hs.584511; -.
DR   ProteinModelPortal; Q9HD64; -.
DR   BioGrid; 575500; 6.
DR   BioGrid; 575617; 36.
DR   IntAct; Q9HD64; 4.
DR   STRING; 9606.ENSP00000364766; -.
DR   PhosphoSite; Q9HD64; -.
DR   DMDM; 334302901; -.
DR   MaxQB; Q9HD64; -.
DR   PaxDb; Q9HD64; -.
DR   PRIDE; Q9HD64; -.
DR   DNASU; 9503; -.
DR   Ensembl; ENST00000375600; ENSP00000364750; ENSG00000204379. [Q9HD64-4]
DR   Ensembl; ENST00000375602; ENSP00000364752; ENSG00000204379. [Q9HD64-2]
DR   Ensembl; ENST00000375613; ENSP00000364763; ENSG00000204382. [Q9HD64-4]
DR   Ensembl; ENST00000375616; ENSP00000364766; ENSG00000204382. [Q9HD64-2]
DR   Ensembl; ENST00000518075; ENSP00000430130; ENSG00000204382. [Q9HD64-2]
DR   GeneID; 653067; -.
DR   GeneID; 653220; -.
DR   KEGG; hsa:653067; -.
DR   KEGG; hsa:653220; -.
DR   UCSC; uc004dqf.3; human. [Q9HD64-2]
DR   UCSC; uc004dqg.3; human. [Q9HD64-4]
DR   CTD; 653067; -.
DR   CTD; 653220; -.
DR   GeneCards; GC00U936381; -.
DR   GeneCards; GC00U936444; -.
DR   GeneCards; GC0XM052512; -.
DR   GeneCards; GC0XP052495; -.
DR   GeneCards; GC0XP052496; -.
DR   HGNC; HGNC:4111; XAGE1A.
DR   HGNC; HGNC:25400; XAGE1B.
DR   HGNC; HGNC:30679; XAGE1C.
DR   HGNC; HGNC:21508; XAGE1D.
DR   HGNC; HGNC:18372; XAGE1E.
DR   MIM; 300289; gene.
DR   MIM; 300742; gene.
DR   MIM; 300743; gene.
DR   MIM; 300744; gene.
DR   MIM; 300745; gene.
DR   neXtProt; NX_Q9HD64; -.
DR   PharmGKB; PA28526; -.
DR   eggNOG; NOG81371; -.
DR   GeneTree; ENSGT00390000003361; -.
DR   InParanoid; Q9HD64; -.
DR   OMA; VICKSCV; -.
DR   OrthoDB; EOG78PVBW; -.
DR   TreeFam; TF342350; -.
DR   NextBio; 122635; -.
DR   PRO; PR:Q9HD64; -.
DR   Proteomes; UP000005640; Chromosome X.
DR   Bgee; Q9HD64; -.
DR   CleanEx; HS_XAGE1D; -.
DR   Genevisible; Q9HD64; HS.
DR   InterPro; IPR008625; GAGE_fam.
DR   PANTHER; PTHR14047; PTHR14047; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; Reference proteome.
FT   CHAIN         1     81       X antigen family member 1.
FT                                /FTId=PRO_0000148349.
FT   VAR_SEQ      33     81       CATWKVICKSCISQTPGINLDLGSGVKVKIIPKEEHCKMPE
FT                                AGEEQPQV -> VLGREMRDMEGDLQELHQSNTGDKSGFGF
FT                                RRQGEDNT (in isoform D).
FT                                {ECO:0000303|PubMed:11992404}.
FT                                /FTId=VSP_001596.
SQ   SEQUENCE   81 AA;  9078 MW;  C73337C4C94C01D1 CRC64;
     MESPKKKNQQ LKVGILHLGS RQKKIRIQLR SQCATWKVIC KSCISQTPGI NLDLGSGVKV
     KIIPKEEHCK MPEAGEEQPQ V
//