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Q9HD64 (XAGE1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
X antigen family member 1

Short name=XAGE-1
Alternative name(s):
Cancer/testis antigen 12.1
Short name=CT12.1
G antigen family D member 2
Gene names
Name:XAGE1A
Synonyms:GAGED2, XAGE1
AND
Name:XAGE1B
AND
Name:XAGE1C
AND
Name:XAGE1D
AND
Name:XAGE1E
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length81 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Tissue specificity

In normal tissues, highly expressed in testis. Expressed also in many different types of cancers: highly expressed in breast cancer, prostate cancer and many types of lung cancers, including squamous cell carcinoma, small cell carcinoma, non-small cell carcinoma, and adenocarcinoma, as well as in Ewing's cell lines, in some Ewing's sarcoma patient samples, and in one of one alveolar rhabdomyosarcoma patient sample. Ref.6 Ref.7

Miscellaneous

According to Ref.2, the transcription of XAGE1A is regulated by methylation of the CpG island in the promoter, and four alternative RNA splicing variants, XAGE-1a, b, c have been identified.

Sequence similarities

Belongs to the GAGE family.

Caution

Ref.7, examines the translation products of theses four XAGE1A transcripts, and finds that the XAGE-1c transcript is possibly translated to 9- and 17-aa polypeptides and not to a protein consisting of 160 amino acids as shown in Ref.2.

Sequence caution

The sequence AAG01401.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAH09538.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAC82986.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAI40888.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI40895.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI41526.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI41531.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI41534.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: Q9HD64-2)

Also known as: XAGE-1a; XAGE-1b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: XAGE-1a and XAGE-1b mRNAs are produced by alternative promoter usage. However, for both isoforms, the translation initiator codon remains the same, generating an identical protein. XAGE-1b is the predominant transcript, compared to XAGE-1a.
Isoform D (identifier: Q9HD64-4)

Also known as: XAGE-1d;

The sequence of this isoform differs from the canonical sequence as follows:
     33-81: CATWKVICKS...PEAGEEQPQV → VLGREMRDME...GFRRQGEDNT

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8181X antigen family member 1
PRO_0000148349

Natural variations

Alternative sequence33 – 8149CATWK…EQPQV → VLGREMRDMEGDLQELHQSN TGDKSGFGFRRQGEDNT in isoform D.
VSP_001596

Sequences

Sequence LengthMass (Da)Tools
Isoform B (XAGE-1a) (XAGE-1b) [UniParc].

Last modified May 31, 2011. Version 3.
Checksum: C73337C4C94C01D1

FASTA819,078
        10         20         30         40         50         60 
MESPKKKNQQ LKVGILHLGS RQKKIRIQLR SQCATWKVIC KSCISQTPGI NLDLGSGVKV 

        70         80 
KIIPKEEHCK MPEAGEEQPQ V 

« Hide

Isoform D (XAGE-1d) [UniParc].

Checksum: 0480A160F893AF81
Show »

FASTA697,965

References

« Hide 'large scale' references
[1]"XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma."
Liu X.F., Helman L.J., Yeung C., Bera T.K., Lee B., Pastan I.
Cancer Res. 60:4752-4755(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
Tissue: Testis.
[2]"The XAGE family of cancer/testis-associated genes: alignment and expression profile in normal tissues, melanoma lesions and Ewing's sarcoma."
Zendman A.J.W., van Kraats A.A., Weidle U.H., Ruiter D.J., van Muijen G.N.P.
Int. J. Cancer 99:361-369(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS B AND D).
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
[4]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Tissue: Lung.
[6]"Characterization of overlapping XAGE-1 transcripts encoding a cancer testis antigen expressed in lung, breast, and other types of cancers."
Egland K.A., Kumar V., Duray P., Pastan I.
Mol. Cancer Ther. 1:441-450(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, IDENTIFICATION OF INITIATOR METHIONINE (ISOFORM B).
[7]"Identification of XAGE-1 isoforms: predominant expression of XAGE-1b in testis and tumors."
Sato S., Noguchi Y., Ohara N., Uenaka A., Shimono M., Nakagawa K., Koizumi F., Ishida T., Yoshino T., Shiratori Y., Nakayama E.
Cancer Immun. 7:5-5(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORMS B AND D), IDENTIFICATION OF INITIATOR METHIONINE (ISOFORM B).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF251237 mRNA. Translation: AAG01401.1. Different initiation.
AJ290447 mRNA. Translation: CAC38107.1.
AJ400997 Genomic DNA. Translation: CAC38108.1.
AJ318878 mRNA. Translation: CAC82986.1. Different initiation.
AJ318879 mRNA. Translation: CAC82987.1.
BT007099 mRNA. Translation: AAP35763.1.
AL772246 Genomic DNA. Translation: CAI41526.1. Sequence problems.
AL772246 Genomic DNA. Translation: CAI41527.1.
AL772246 Genomic DNA. Translation: CAI41528.1.
AL772246 Genomic DNA. Translation: CAI41530.1.
AL772246 Genomic DNA. Translation: CAI41531.1. Sequence problems.
AL772246 Genomic DNA. Translation: CAI41532.1.
AL772246 Genomic DNA. Translation: CAI41533.1.
AL772246 Genomic DNA. Translation: CAI41534.1. Sequence problems.
BX088602 Genomic DNA. Translation: CAI40888.1. Sequence problems.
BX088602 Genomic DNA. Translation: CAI40889.1.
BX088602 Genomic DNA. Translation: CAI40890.1.
BX088602 Genomic DNA. Translation: CAI40893.1.
BX088602 Genomic DNA. Translation: CAI40894.1.
BX088602 Genomic DNA. Translation: CAI40895.1. Sequence problems.
BC009538 mRNA. Translation: AAH09538.2. Different initiation.
RefSeqNP_001091061.2. NM_001097592.2.
NP_001091062.1. NM_001097593.2.
NP_001091063.2. NM_001097594.2.
NP_001091065.1. NM_001097596.2.
NP_001091066.2. NM_001097597.2.
NP_001091067.1. NM_001097598.2.
NP_001091073.2. NM_001097604.2.
NP_001091074.1. NM_001097605.2.
NP_065144.2. NM_020411.2.
NP_597673.1. NM_133430.2.
UniGeneHs.112208.
Hs.546096.
Hs.584511.

3D structure databases

ProteinModelPortalQ9HD64.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114882. 2 interactions.
575486. 1 interaction.
IntActQ9HD64. 1 interaction.
STRING9606.ENSP00000342190.

PTM databases

PhosphoSiteQ9HD64.

Polymorphism databases

DMDM334302901.

Proteomic databases

PaxDbQ9HD64.
PRIDEQ9HD64.

Protocols and materials databases

DNASU9503.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000374959; ENSP00000364097; ENSG00000204376. [Q9HD64-4]
ENST00000375567; ENSP00000364717; ENSG00000204375. [Q9HD64-4]
ENST00000375570; ENSP00000364720; ENSG00000204375. [Q9HD64-2]
ENST00000375578; ENSP00000364728; ENSG00000204376. [Q9HD64-2]
ENST00000375588; ENSP00000364738; ENSG00000183461. [Q9HD64-4]
ENST00000375589; ENSP00000364739; ENSG00000183461. [Q9HD64-2]
ENST00000375600; ENSP00000364750; ENSG00000204379. [Q9HD64-4]
ENST00000375602; ENSP00000364752; ENSG00000204379. [Q9HD64-2]
ENST00000375613; ENSP00000364763; ENSG00000204382. [Q9HD64-4]
ENST00000375616; ENSP00000364766; ENSG00000204382. [Q9HD64-2]
ENST00000396497; ENSP00000379755; ENSG00000204376.
ENST00000399795; ENSP00000382693; ENSG00000183461.
ENST00000399800; ENSP00000382698; ENSG00000204379.
ENST00000399805; ENSP00000382704; ENSG00000204382. [Q9HD64-4]
ENST00000429372; ENSP00000389983; ENSG00000204375.
ENST00000437949; ENSP00000388178; ENSG00000204382.
ENST00000438079; ENSP00000389220; ENSG00000204376. [Q9HD64-4]
ENST00000441417; ENSP00000413903; ENSG00000204379. [Q9HD64-4]
ENST00000446098; ENSP00000409639; ENSG00000183461. [Q9HD64-4]
ENST00000518075; ENSP00000430130; ENSG00000204382. [Q9HD64-2]
ENST00000594178; ENSP00000470774; ENSG00000262401.
ENST00000596140; ENSP00000469498; ENSG00000262401. [Q9HD64-2]
ENST00000597159; ENSP00000470293; ENSG00000262401. [Q9HD64-2]
ENST00000597405; ENSP00000470080; ENSG00000269805. [Q9HD64-4]
ENST00000597878; ENSP00000472622; ENSG00000269805. [Q9HD64-2]
ENST00000598106; ENSP00000471993; ENSG00000262401. [Q9HD64-4]
ENST00000600138; ENSP00000469109; ENSG00000269805.
ENST00000600638; ENSP00000469147; ENSG00000262401. [Q9HD64-4]
ENST00000602068; ENSP00000469100; ENSG00000269805. [Q9HD64-4]
GeneID653048.
653067.
653219.
653220.
9503.
KEGGhsa:653048.
hsa:653067.
hsa:653219.
hsa:653220.
hsa:9503.
UCSCuc004dqf.3. human. [Q9HD64-2]
uc004dqg.3. human. [Q9HD64-4]

Organism-specific databases

CTD653048.
653067.
653219.
653220.
9503.
GeneCardsGC0XM052255.
GC0XM052545.
GC0XM052559.
GC0XP052239.
GC0XP052511.
HGNCHGNC:4111. XAGE1A.
HGNC:25400. XAGE1B.
HGNC:30679. XAGE1C.
HGNC:21508. XAGE1D.
HGNC:18372. XAGE1E.
HPAHPA050858.
MIM300289. gene.
300742. gene.
300743. gene.
300744. gene.
300745. gene.
neXtProtNX_Q9HD64.
PharmGKBPA28526.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG81371.
InParanoidQ9HD64.
OrthoDBEOG78PVBW.
TreeFamTF342350.

Gene expression databases

BgeeQ9HD64.
CleanExHS_XAGE1D.
GenevestigatorQ9HD64.

Family and domain databases

InterProIPR008625. GAGE.
[Graphical view]
PfamPF05831. GAGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiXAGE1D.
NextBio122635.
PROQ9HD64.
SOURCESearch...

Entry information

Entry nameXAGE1_HUMAN
AccessionPrimary (citable) accession number: Q9HD64
Secondary accession number(s): A6NJ94 expand/collapse secondary AC list , Q5JPN8, Q5JPP0, Q5JPP3, Q8WWG5, Q8WWG6, Q969J6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 31, 2011
Last modified: April 16, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM