Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9HD15

- SRA1_HUMAN

UniProt

Q9HD15 - SRA1_HUMAN

Protein

Steroid receptor RNA activator 1

Gene

SRA1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.5 Publications

    GO - Molecular functioni

    1. DNA binding Source: Ensembl
    2. ligand-dependent nuclear receptor transcription coactivator activity Source: RefGenome
    3. protein binding Source: IntAct
    4. thyroid hormone receptor activator activity Source: Ensembl
    5. transcription coactivator activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cell differentiation Source: UniProtKB
    3. cell proliferation Source: UniProtKB
    4. positive regulation of receptor activity Source: RefGenome
    5. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    6. regulation of apoptotic process Source: UniProtKB
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Receptor, Ribonucleoprotein

    Keywords - Biological processi

    Apoptosis, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid receptor RNA activator 1
    Alternative name(s):
    Steroid receptor RNA activator protein
    Short name:
    SRAP
    Gene namesi
    Name:SRA1Imported
    ORF Names:PP7684
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:11281. SRA1.

    Subcellular locationi

    Nucleus 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cell leading edge Source: Ensembl
    2. cytoplasm Source: HPA
    3. intercellular bridge Source: HPA
    4. microtubule cytoskeleton Source: HPA
    5. nucleus Source: UniProtKB
    6. plasma membrane Source: HPA
    7. ribonucleoprotein complex Source: UniProtKB
    8. transcription factor complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36110.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 236236Steroid receptor RNA activator 1PRO_0000234105Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei60 – 601Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9HD15.
    PaxDbiQ9HD15.
    PeptideAtlasiQ9HD15.
    PRIDEiQ9HD15.

    PTM databases

    PhosphoSiteiQ9HD15.

    Expressioni

    Tissue specificityi

    Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.3 Publications

    Gene expression databases

    ArrayExpressiQ9HD15.
    BgeeiQ9HD15.
    CleanExiHS_SRA1.
    GenevestigatoriQ9HD15.

    Organism-specific databases

    HPAiHPA044598.
    HPA050153.

    Interactioni

    Subunit structurei

    SRA1 RNA exists in a ribonucleoprotein complex containing NCOA1. The RNA also forms a complex with PUS1 and RARG in the nucleus. Interacts with AR.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HDAC2Q927692EBI-727136,EBI-301821

    Protein-protein interaction databases

    BioGridi115329. 39 interactions.
    IntActiQ9HD15. 2 interactions.
    MINTiMINT-1383311.
    STRINGi9606.ENSP00000337513.

    Structurei

    Secondary structure

    1
    236
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi111 – 12414
    Turni125 – 1284
    Helixi131 – 15020
    Helixi155 – 16915
    Helixi173 – 19119
    Turni192 – 1943
    Helixi195 – 20915
    Turni221 – 2233
    Beta strandi231 – 2344

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2MGXNMR-A107-236[»]
    4NBOX-ray2.81A/B106-215[»]
    ProteinModelPortaliQ9HD15.
    SMRiQ9HD15. Positions 107-236.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi56 – 10449Pro-richSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SRA1 family.Curated

    Phylogenomic databases

    eggNOGiNOG44276.
    HOVERGENiHBG061820.
    InParanoidiQ9HD15.
    OMAiQTQTGGP.
    OrthoDBiEOG790G2V.
    PhylomeDBiQ9HD15.
    TreeFamiTF314789.

    Family and domain databases

    InterProiIPR009917. SRA1-protein/COPII_Sec31.
    [Graphical view]
    PfamiPF07304. SRA1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HD15-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTRCPAGQAE VEMAELYVKP GNKERGWNDP PQFSYGLQTQ AGGPRRSLLT    50
    KRVAAPQDGS PRVPASETSP GPPPMGPPPP SSKAPRSPPV GSGPASGVEP 100
    TSFPVESEAV MEDVLRPLEQ ALEDCRGHTR KQVCDDISRR LALLQEQWAG 150
    GKLSIPVKKR MALLVQELSS HRWDAADDIH RSLMVDHVTE VSQWMVGVKR 200
    LIAEKRSLFS EEAANEEKSA ATAEKNHTIP GFQQAS 236
    Length:236
    Mass (Da):25,673
    Last modified:March 1, 2001 - v1
    Checksum:i9B0B60BE30ADD263
    GO

    Sequence cautioni

    The sequence AAH40043.2 differs from that shown. Reason: Frameshift at position 29.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 132EM → RL in AF092038. (PubMed:10199399)Curated
    Sequence conflicti29 – 291D → T in AAH40043. (PubMed:15489334)Curated
    Sequence conflicti50 – 501T → I in AAG02115. (PubMed:12565891)Curated
    Sequence conflicti110 – 1101V → RL in AAG02116. (PubMed:12565891)Curated
    Sequence conflicti110 – 1101V → RL in AAL55868. (PubMed:15498874)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti32 – 321Q → E.
    Corresponds to variant rs35610885 [ dbSNP | Ensembl ].
    VAR_052060

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF293024 mRNA. Translation: AAG02114.1.
    AF293025 mRNA. Translation: AAG02115.1.
    AF293026 mRNA. Translation: AAG02116.1.
    AF318361 mRNA. Translation: AAL55868.1.
    AF092038 mRNA. No translation available.
    BC040043 mRNA. Translation: AAH40043.2. Frameshift.
    CCDSiCCDS34245.1.
    RefSeqiNP_001030312.2. NM_001035235.3.
    UniGeneiHs.653135.

    Genome annotation databases

    EnsembliENST00000336283; ENSP00000337513; ENSG00000213523.
    GeneIDi10011.
    KEGGihsa:10011.
    UCSCiuc003lfz.3. human.

    Polymorphism databases

    DMDMi74718904.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF293024 mRNA. Translation: AAG02114.1 .
    AF293025 mRNA. Translation: AAG02115.1 .
    AF293026 mRNA. Translation: AAG02116.1 .
    AF318361 mRNA. Translation: AAL55868.1 .
    AF092038 mRNA. No translation available.
    BC040043 mRNA. Translation: AAH40043.2 . Frameshift.
    CCDSi CCDS34245.1.
    RefSeqi NP_001030312.2. NM_001035235.3.
    UniGenei Hs.653135.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2MGX NMR - A 107-236 [» ]
    4NBO X-ray 2.81 A/B 106-215 [» ]
    ProteinModelPortali Q9HD15.
    SMRi Q9HD15. Positions 107-236.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115329. 39 interactions.
    IntActi Q9HD15. 2 interactions.
    MINTi MINT-1383311.
    STRINGi 9606.ENSP00000337513.

    PTM databases

    PhosphoSitei Q9HD15.

    Polymorphism databases

    DMDMi 74718904.

    Proteomic databases

    MaxQBi Q9HD15.
    PaxDbi Q9HD15.
    PeptideAtlasi Q9HD15.
    PRIDEi Q9HD15.

    Protocols and materials databases

    DNASUi 10011.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000336283 ; ENSP00000337513 ; ENSG00000213523 .
    GeneIDi 10011.
    KEGGi hsa:10011.
    UCSCi uc003lfz.3. human.

    Organism-specific databases

    CTDi 10011.
    GeneCardsi GC05M139911.
    HGNCi HGNC:11281. SRA1.
    HPAi HPA044598.
    HPA050153.
    MIMi 603819. gene.
    neXtProti NX_Q9HD15.
    PharmGKBi PA36110.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG44276.
    HOVERGENi HBG061820.
    InParanoidi Q9HD15.
    OMAi QTQTGGP.
    OrthoDBi EOG790G2V.
    PhylomeDBi Q9HD15.
    TreeFami TF314789.

    Miscellaneous databases

    GeneWikii SRA1.
    GenomeRNAii 10011.
    NextBioi 37823.
    PROi Q9HD15.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9HD15.
    Bgeei Q9HD15.
    CleanExi HS_SRA1.
    Genevestigatori Q9HD15.

    Family and domain databases

    InterProi IPR009917. SRA1-protein/COPII_Sec31.
    [Graphical view ]
    Pfami PF07304. SRA1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Mammary glandImported.
    2. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
      Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
      , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
      Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "A steroid receptor coactivator, SRA, functions as an RNA and is present in an SRC-1 complex."
      Lanz R.B., McKenna N.J., Onate S.A., Albrecht U., Wong J., Tsai S.Y., Tsai M.-J., O'Malley B.W.
      Cell 97:17-27(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-236, FUNCTION, IDENTIFICATION IN A RIBONUCLEOPROTEIN COMPLEX WITH NCOA1, TISSUE SPECIFICITY.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 22-236.
      Tissue: LymphImported.
    5. "Ligand-independent coactivation of ERalpha AF-1 by steroid receptor RNA activator (SRA) via MAPK activation."
      Deblois G., Giguere V.
      J. Steroid Biochem. Mol. Biol. 85:123-131(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Steroid receptor RNA activator stimulates proliferation as well as apoptosis in vivo."
      Lanz R.B., Chua S.S., Barron N., Soder B.M., DeMayo F., O'Malley B.W.
      Mol. Cell. Biol. 23:7163-7176(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    7. "SRA coactivation of estrogen receptor-alpha is phosphorylation-independent, and enhances 4-hydroxytamoxifen agonist activity."
      Coleman K.M., Lam V., Jaber B.M., Lanz R.B., Smith C.L.
      Biochem. Biophys. Res. Commun. 323:332-338(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The steroid receptor RNA activator is the first functional RNA encoding a protein."
      Chooniedass-Kothari S., Emberley E., Hamedani M.K., Troup S., Wang X., Czosnek A., Hube F., Mutawe M., Watson P.H., Leygue E.
      FEBS Lett. 566:43-47(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSRA1_HUMAN
    AccessioniPrimary (citable) accession number: Q9HD15
    Secondary accession number(s): Q6NVU9
    , Q8IXM1, Q9HD13, Q9HD14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Appears to be the first example of a new class of functional RNAs also able to encode a protein.2 Publications

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3