Q9HCY8 (S10AE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A14

Alternative name(s):
S100 calcium-binding protein A14
Short name=S114

Gene names

Name:	S100A14
Synonyms:	S100A15

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulates P53/TP53 protein levels, and thereby plays a role in the regulation of cell survival and apoptosis. Depending on the context, it can promote cell proliferation or apoptosis. Plays a role in the regulation of cell migration by modulating the levels of MMP2, a matrix protease that is under transcriptional control of P53/TP53. Does not bind calcium. Ref.5 Ref.6 Ref.7
Subunit structure	Homodimer. Interacts with AGER. Ref.5 Ref.8
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Expressed at highest levels in colon and at moderate levels in thymus, kidney, liver, small intestine, and lung. Low expression in heart and no expression is seen in brain, skeletal muscle, spleen, placenta and peripheral blood leukocytes. Ref.1
Sequence similarities	Belongs to the S-100 family. Contains 1 EF-hand domain.
Caution	Part of the residues that are essential for calcium binding are not conserved, resulting in loss of calcium binding at physiological calcium concentrations (Ref.8).

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion homeostasis Non-traceable author statement PubMed 16682778. Source: UniProtKB defense response to bacterium Inferred from expression pattern PubMed 17625598. Source: UniProtKB positive regulation of granulocyte chemotaxis Inferred from direct assay PubMed 18606705. Source: UniProtKB positive regulation of monocyte chemotaxis Inferred from direct assay PubMed 18606705. Source: UniProtKB response to lipopolysaccharide Inferred from expression pattern PubMed 17625598. Source: UniProtKB toll-like receptor 4 signaling pathway Inferred from direct assay PubMed 17625598. Source: UniProtKB
Cellular_component	cell junction Inferred from direct assay. Source: HPA microtubule cytoskeleton Inferred from direct assay. Source: HPA perinuclear region of cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	calcium ion binding Non-traceable author statement PubMed 16682778. Source: UniProtKB chemokine receptor binding Inferred from mutant phenotype PubMed 18606705. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 104

104

Protein S100-A14

PRO_0000144021

Regions

Domain

27 – 61

35

EF-hand

Secondary structure

1

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104

Helix Strand Turn

Details...

Beta strand

8 – 11

4

Helix

19 – 32

14

Turn

34 – 39

6

Helix

43 – 53

11

Turn

55 – 57

3

Helix

60 – 62

3

Helix

65 – 71

7

Helix

81 – 94

14

Beta strand

98 – 100

3

Sequences

	Sequence		Length	Mass (Da)
	Q9HCY8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 97EF31A46B388E79	FASTA	104	11,662
	10 20 30 40 50 60 MGQCRSANAE DAQEFSDVER AIETLIKNFH QYSVEGGKET LTPSELRDLV TQQLPHLMPS 70 80 90 100 NCGLEEKIAN LGSCNDSKLE FRSFWELIGE AAKSVKLERP VRGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the human S100A14 gene encoding a novel member of the S100 family." Pietas A., Schluns K., Marenholz I., Schafer B.W., Heizmann C.W., Petersen I. Genomics 79:513-522(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]	"S100A14 stimulates cell proliferation and induces cell apoptosis at different concentrations via receptor for advanced glycation end products (RAGE)." Jin Q., Chen H., Luo A., Ding F., Liu Z. PLoS ONE 6:E19375-E19375(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AGER.
[6]	"Involvement of S100A14 protein in cell invasion by affecting expression and function of matrix metalloproteinase (MMP)-2 via p53-dependent transcriptional regulation." Chen H., Yuan Y., Zhang C., Luo A., Ding F., Ma J., Yang S., Tian Y., Tong T., Zhan Q., Liu Z. J. Biol. Chem. 287:17109-17119(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"S100A14 inhibits proliferation of oral carcinoma derived cells through G1-arrest." Sapkota D., Costea D.E., Blo M., Bruland O., Lorens J.B., Vasstrand E.N., Ibrahim S.O. Oral Oncol. 48:219-225(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Solution structure and dynamics of human S100A14." Bertini I., Borsi V., Cerofolini L., Das Gupta S., Fragai M., Luchinat C. J. Biol. Inorg. Chem. 18:183-194(2013) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, ABSENCE OF CALCIUM BINDING, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY007220 mRNA. Translation: AAG01893.1.
AF426828 Genomic DNA. Translation: AAM19206.1.
BX470102 Genomic DNA. Translation: CAI14759.1.
BC005019 mRNA. Translation: AAH05019.1.

IPI

IPI00010214.

RefSeq

NP_065723.1. NM_020672.2.

UniGene

Hs.288998.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2M0R	NMR	-	A/B	1-104	[»]

ProteinModelPortal

Q9HCY8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9HCY8. 1 interaction.

MINT

MINT-5002007.

STRING

9606.ENSP00000340463.

PTM databases

PhosphoSite

Q9HCY8.

Polymorphism databases

DMDM

20178118.

Proteomic databases

PaxDb

Q9HCY8.

PeptideAtlas

Q9HCY8.

PRIDE

Q9HCY8.

Protocols and materials databases

DNASU

57402.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000344616; ENSP00000340463; ENSG00000189334.
ENST00000368701; ENSP00000357690; ENSG00000189334.
ENST00000368702; ENSP00000357691; ENSG00000189334.
ENST00000476873; ENSP00000420296; ENSG00000189334.

GeneID

57402.

KEGG

hsa:57402.

UCSC

uc001fce.3. human.

Organism-specific databases

CTD

57402.

GeneCards

GC01M153586.

HGNC

HGNC:18901. S100A14.

HPA

HPA027613.

MIM

607986. gene.

neXtProt

NX_Q9HCY8.

PharmGKB

PA134905502.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG46731.

HOGENOM

HOG000246968.

HOVERGEN

HBG001479.

InParanoid

Q9HCY8.

OMA

IANLGNC.

OrthoDB

EOG4WSWC3.

PhylomeDB

Q9HCY8.

Gene expression databases

Bgee

Q9HCY8.

CleanEx

HS_S100A14.

Genevestigator

Q9HCY8.

GermOnline

ENSG00000189334. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR013787. S100_Ca-bd_sub.
[Graphical view]

Pfam

PF01023. S_100. 1 hit.
[Graphical view]

PROSITE

PS50222. EF_HAND_2. False negative.
PS00303. S100_CABP. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

S100A14. human.

GenomeRNAi

57402.

NextBio

63504.

SOURCE

Search...

Entry information

Entry name

S10AE_HUMAN

Accession

Primary (citable) accession number: Q9HCY8
Secondary accession number(s): Q5RHT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.