Q9HCX4 (TRPC7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Short transient receptor potential channel 7
Short name=TrpC7

Alternative name(s):
Transient receptor protein 7
Short name=TRP-7
Short name=hTRP7

Gene names

Name:	TRPC7
Synonyms:	TRP7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) By similarity. May also be activated by intracellular calcium store depletion.
Subunit structure	Interacts with MX1 and RNF24. Interacts (via ANK-repeat domains) with PRKG1. Ref.6 Ref.7 Ref.8
Subcellular location	Cell membrane; Multi-pass membrane protein. Nucleus envelope Ref.8.
Post-translational modification	Phosphorylation by PRKG1 at Thr-15 negatively regulates TRPC7 activity.
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC7 sub-subfamily. [View classification] Contains 4 ANK repeats.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell membrane Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ion channel
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Traceable author statement. Source: Reactome manganese ion transport Inferred from electronic annotation. Source: Compara platelet activation Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: Reactome
Molecular_function	calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9HCX4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9HCX4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     260-375: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9HCX4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     260-320: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	Short transient receptor potential channel 7		PRO_0000215324
Regions
	Topological domain	1 – 351	351	Cytoplasmic Potential
	Transmembrane	352 – 372	21	Helical; Potential
	Topological domain	373 – 383	11	Extracellular Potential
	Transmembrane	384 – 404	21	Helical; Potential
	Topological domain	405 – 465	61	Cytoplasmic Potential
	Transmembrane	466 – 486	21	Helical; Potential
	Topological domain	487 – 537	51	Extracellular Potential
	Transmembrane	538 – 558	21	Helical; Potential
	Topological domain	559 – 581	23	Cytoplasmic Potential
	Transmembrane	582 – 602	21	Helical; Potential
	Topological domain	603 – 651	49	Extracellular Potential
	Transmembrane	652 – 672	21	Helical; Potential
	Topological domain	673 – 862	190	Cytoplasmic Potential
	Repeat	42 – 71	30	ANK 1
	Repeat	77 – 106	30	ANK 2
	Repeat	108 – 134	27	ANK 3
	Repeat	163 – 192	30	ANK 4
Amino acid modifications
	Modified residue	15	1	Phosphothreonine; by PKG/PRKG1 Ref.8
	Glycosylation	514	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	260 – 375	116	Missing in isoform 2.		VSP_043035
	Alternative sequence	260 – 320	61	Missing in isoform 3.		VSP_044897
Experimental info
	Sequence conflict	412	1	G → S in CAD70161. Ref.3
	Sequence conflict	486	1	F → S in CAD70161. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C516E59E80AEF4B0	FASTA	862	99,562
	10 20 30 40 50 60 MLRNSTFKNM QRRHTTLREK GRRQAIRGPA YMFNEKGTSL TPEEERFLDS AEYGNIPVVR 70 80 90 100 110 120 KMLEESKTLN FNCVDYMGQN ALQLAVGNEH LEVTELLLKK ENLARVGDAL LLAISKGYVR 130 140 150 160 170 180 IVEAILNHPA FAQGQRLTLS PLEQELRDDD FYAYDEDGTR FSHDITPIIL AAHCQEYEIV 190 200 210 220 230 240 HILLLKGARI ERPHDYFCKC NECTEKQRKD SFSHSRSRMN AYKGLASAAY LSLSSEDPVL 250 260 270 280 290 300 TALELSNELA RLANIETEFK NDYRKLSMQC KDFVVGVLDL CRDTEEVEAI LNGDVNFQVW 310 320 330 340 350 360 SDHHRPSLSR IKLAIKYEVK KFVAHPNCQQ QLLTMWYENL SGLRQQSIAV KFLAVFGVSI 370 380 390 400 410 420 GLPFLAIAYW IAPCSKLGRT LRSPFMKFVA HAVSFTIFLG LLVVNASDRF EGVKTLPNET 430 440 450 460 470 480 FTDYPKQIFR VKTTQFSWTE MLIMKWVLGM IWSECKEIWE EGPREYVLHL WNLLDFGMLS 490 500 510 520 530 540 IFVASFTARF MAFLKATEAQ LYVDQHVQDD TLHNVSLPPE VAYFTYARDK WWPSDPQIIS 550 560 570 580 590 600 EGLYAIAVVL SFSRIAYILP ANESFGPLQI SLGRTVKDIF KFMVIFIMVF VAFMIGMFNL 610 620 630 640 650 660 YSYYRGAKYN PAFTTVEESF KTLFWSIFGL SEVISVVLKY DHKFIENIGY VLYGVYNVTM 670 680 690 700 710 720 VVVLLNMLIA MINNSYQEIE EDADVEWKFA RAKLWLSYFD EGRTLPAPFN LVPSPKSFYY 730 740 750 760 770 780 LIMRIKMCLI KLCKSKAKSC ENDLEMGMLN SKFKKTRYQA GMRNSENLTA NNTLSKPTRY 790 800 810 820 830 840 QKIMKRLIKR YVLKAQVDRE NDEVNEGELK EIKQDISSLR YELLEEKSQA TGELADLIQQ 850 860 LSEKFGKNLN KDHLRVNKGK DI
	Isoform 2 [UniParc]. Checksum: DBF64B392022D797	FASTA	746	86,277
	10 20 30 40 50 60 MLRNSTFKNM QRRHTTLREK GRRQAIRGPA YMFNEKGTSL TPEEERFLDS AEYGNIPVVR 70 80 90 100 110 120 KMLEESKTLN FNCVDYMGQN ALQLAVGNEH LEVTELLLKK ENLARVGDAL LLAISKGYVR 130 140 150 160 170 180 IVEAILNHPA FAQGQRLTLS PLEQELRDDD FYAYDEDGTR FSHDITPIIL AAHCQEYEIV 190 200 210 220 230 240 HILLLKGARI ERPHDYFCKC NECTEKQRKD SFSHSRSRMN AYKGLASAAY LSLSSEDPVL 250 260 270 280 290 300 TALELSNELA RLANIETEFK LGRTLRSPFM KFVAHAVSFT IFLGLLVVNA SDRFEGVKTL 310 320 330 340 350 360 PNETFTDYPK QIFRVKTTQF SWTEMLIMKW VLGMIWSECK EIWEEGPREY VLHLWNLLDF 370 380 390 400 410 420 GMLSIFVASF TARFMAFLKA TEAQLYVDQH VQDDTLHNVS LPPEVAYFTY ARDKWWPSDP 430 440 450 460 470 480 QIISEGLYAI AVVLSFSRIA YILPANESFG PLQISLGRTV KDIFKFMVIF IMVFVAFMIG 490 500 510 520 530 540 MFNLYSYYRG AKYNPAFTTV EESFKTLFWS IFGLSEVISV VLKYDHKFIE NIGYVLYGVY 550 560 570 580 590 600 NVTMVVVLLN MLIAMINNSY QEIEEDADVE WKFARAKLWL SYFDEGRTLP APFNLVPSPK 610 620 630 640 650 660 SFYYLIMRIK MCLIKLCKSK AKSCENDLEM GMLNSKFKKT RYQAGMRNSE NLTANNTLSK 670 680 690 700 710 720 PTRYQKIMKR LIKRYVLKAQ VDRENDEVNE GELKEIKQDI SSLRYELLEE KSQATGELAD 730 740 LIQQLSEKFG KNLNKDHLRV NKGKDI
	Isoform 3 [UniParc]. Checksum: 01106D703524DA00	FASTA	801	92,429
	10 20 30 40 50 60 MLRNSTFKNM QRRHTTLREK GRRQAIRGPA YMFNEKGTSL TPEEERFLDS AEYGNIPVVR 70 80 90 100 110 120 KMLEESKTLN FNCVDYMGQN ALQLAVGNEH LEVTELLLKK ENLARVGDAL LLAISKGYVR 130 140 150 160 170 180 IVEAILNHPA FAQGQRLTLS PLEQELRDDD FYAYDEDGTR FSHDITPIIL AAHCQEYEIV 190 200 210 220 230 240 HILLLKGARI ERPHDYFCKC NECTEKQRKD SFSHSRSRMN AYKGLASAAY LSLSSEDPVL 250 260 270 280 290 300 TALELSNELA RLANIETEFK FVAHPNCQQQ LLTMWYENLS GLRQQSIAVK FLAVFGVSIG 310 320 330 340 350 360 LPFLAIAYWI APCSKLGRTL RSPFMKFVAH AVSFTIFLGL LVVNASDRFE GVKTLPNETF 370 380 390 400 410 420 TDYPKQIFRV KTTQFSWTEM LIMKWVLGMI WSECKEIWEE GPREYVLHLW NLLDFGMLSI 430 440 450 460 470 480 FVASFTARFM AFLKATEAQL YVDQHVQDDT LHNVSLPPEV AYFTYARDKW WPSDPQIISE 490 500 510 520 530 540 GLYAIAVVLS FSRIAYILPA NESFGPLQIS LGRTVKDIFK FMVIFIMVFV AFMIGMFNLY 550 560 570 580 590 600 SYYRGAKYNP AFTTVEESFK TLFWSIFGLS EVISVVLKYD HKFIENIGYV LYGVYNVTMV 610 620 630 640 650 660 VVLLNMLIAM INNSYQEIEE DADVEWKFAR AKLWLSYFDE GRTLPAPFNL VPSPKSFYYL 670 680 690 700 710 720 IMRIKMCLIK LCKSKAKSCE NDLEMGMLNS KFKKTRYQAG MRNSENLTAN NTLSKPTRYQ 730 740 750 760 770 780 KIMKRLIKRY VLKAQVDREN DEVNEGELKE IKQDISSLRY ELLEEKSQAT GELADLIQQL 790 800 SEKFGKNLNK DHLRVNKGKD I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distribution of a novel human capacitative calcium entry channel, htrp7." Murphy C.T., Li S., Jordan N.J., Reaves B.J., Wolstenholme A.J., Westwick J. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Localization of TRPC7 and splice variants in human tissues." Xu S.-Z., Beech D.J. Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Tissue distribution, alternative splicing and function of human TRPC7." Aptel H., Murphy C.T., Li S.W., Chen P.B., Rogers A.T., Franklin I., Westwick J., Reaves B.J., Woodward B., Wolstenholme A.J. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Brain.
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"MxA, a member of the dynamin superfamily, interacts with the ankyrin-like repeat domain of TRPC." Lussier M.P., Cayouette S., Lepage P.K., Bernier C.L., Francoeur N., St-Hilaire M., Pinard M., Boulay G. J. Biol. Chem. 280:19393-19400(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MX1.
[7]	"RNF24, a new TRPC interacting protein, causes the intracellular retention of TRPC." Lussier M.P., Lepage P.K., Bousquet S.M., Boulay G. Cell Calcium 43:432-443(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RNF24.
[8]	"Functional regulation of transient receptor potential canonical 7 by cGMP-dependent protein kinase Ialpha." Yuasa K., Matsuda T., Tsuji A. Cell. Signal. 23:1179-1187(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-15 BY PRKG1, INTERACTION WITH PRKG1, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ272034 mRNA. Translation: CAC03489.1. AY167927 mRNA. Translation: AAO12125.1. AJ549088 mRNA. Translation: CAD70161.1. AC008661 Genomic DNA. No translation available. BC128185 mRNA. Translation: AAI28186.1.
IPI	IPI00030930. IPI00335274. IPI00428059.
RefSeq	NP_001161048.1. NM_001167576.1. NP_001161049.1. NM_001167577.1. NP_065122.1. NM_020389.2.
UniGene	Hs.591263.
3D structure databases
ProteinModelPortal	Q9HCX4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HCX4. 1 interaction.
STRING	9606.ENSP00000265193.
PTM databases
PhosphoSite	Q9HCX4.
Polymorphism databases
DMDM	18202960.
Proteomic databases
PaxDb	Q9HCX4.
PRIDE	Q9HCX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355180; ENSP00000347312; ENSG00000069018. ENST00000426057; ENSP00000441628; ENSG00000069018. ENST00000513104; ENSP00000426070; ENSG00000069018.
GeneID	57113.
KEGG	hsa:57113.
UCSC	uc003lbn.2. human.
Organism-specific databases
CTD	57113.
GeneCards	GC05M135549.
HGNC	HGNC:20754. TRPC7.
HPA	CAB022592. HPA031126.
neXtProt	NX_Q9HCX4.
PharmGKB	PA164742693.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG254238.
HOGENOM	HOG000020590.
HOVERGEN	HBG068337.
InParanoid	Q9HCX4.
KO	K04970.
OMA	KKTRYQA.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology. REACT_111102. Signal Transduction. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q9HCX4.
Bgee	Q9HCX4.
CleanEx	HS_TRPC7.
Genevestigator	Q9HCX4.
GermOnline	ENSG00000069018. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans_dom. IPR004729. TRP_channel. IPR013555. TRP_dom. IPR005463. TRPC7_channel. IPR002153. TRPC_channel. [Graphical view]
PANTHER	PTHR10117. PTHR10117. 1 hit. PTHR10117:SF9. PTHR10117:SF9. 1 hit.
Pfam	PF12796. Ank_2. 1 hit. PF00520. Ion_trans. 1 hit. PF08344. TRP_2. 1 hit. [Graphical view]
PRINTS	PR01097. TRNSRECEPTRP. PR01648. TRPCHANNEL7.
SMART	SM00248. ANK. 3 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
TIGRFAMs	TIGR00870. trp. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. False negative. PS50088. ANK_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57113.
NextBio	62971.

Entry information

Entry name

TRPC7_HUMAN

Accession

Primary (citable) accession number: Q9HCX4
Secondary accession number(s): A1A4Z4 , F5H5U9, Q70T26, Q8IWP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.