Q9HCU8 (DPOD4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase delta subunit 4

Alternative name(s):
DNA polymerase delta subunit p12

Gene names

Name:	POLD4
Synonyms:	POLDS

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for optimal DNA polymerase delta activity. May contribute to PCNA-dependent activity of DNA polymerase delta. Ref.4
Subunit structure	Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. Interacts with WRNIP1. Interacts with POLD4, POLD2, POLD1 and PCNA. Ref.3 Ref.4
Subcellular location	Nucleus.
Sequence similarities	Belongs to the DNA polymerase delta subunit 4 family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA strand elongation involved in DNA replication Traceable author statement. Source: Reactome S phase of mitotic cell cycle Traceable author statement. Source: Reactome base-excision repair Traceable author statement. Source: Reactome nucleotide-excision repair, DNA gap filling Traceable author statement. Source: Reactome positive regulation of endothelial cell proliferation Inferred from electronic annotation. Source: Compara telomere maintenance via recombination Traceable author statement. Source: Reactome telomere maintenance via semi-conservative replication Traceable author statement. Source: Reactome transcription-coupled nucleotide-excision repair Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA-directed DNA polymerase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PCNA	P12004	4	EBI-864968,EBI-358311
POLD1	P28340	10	EBI-864968,EBI-716569
POLD2	P49005	3	EBI-864968,EBI-372354
WRNIP1	Q96S55	2	EBI-864968,EBI-2513471

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 107	107	DNA polymerase delta subunit 4		PRO_0000186051
Natural variations
	Natural variant	39	1	R → P. Ref.2 Corresponds to variant rs28364240 [ dbSNP | Ensembl ].		VAR_022269
	Natural variant	59	1	G → R. Corresponds to variant rs34136263 [ dbSNP | Ensembl ].		VAR_057526
Experimental info
	Mutagenesis	7	1	I → A: Abolishes interaction with PCNA; when associated with 10-AA-11. Ref.4
	Mutagenesis	10 – 11	2	SY → A: Abolishes interaction with PCNA; when associated with A-7.

Sequences

	Sequence		Length	Mass (Da)
	Q9HCU8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6F412738E4D9A19C	FASTA	107	12,433
	10 20 30 40 50 60 MGRKRLITDS YPVVKRREGP AGHSKGELAP ELGEEPQPRD EEEAELELLR QFDLAWQYGP 70 80 90 100 CTGITRLQRW CRAKQMGLEP PPEVWQVLKT HPGDPRFQCS LWHLYPL

References

[1]	"Identification of a fourth subunit of mammalian DNA polymerase delta." Liu L., Mo J.-Y., Rodriguez-Belmonte E.M., Lee M.Y.W.T. J. Biol. Chem. 275:18739-18744(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovarian carcinoma.
[2]	NIEHS SNPs program Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT PRO-39.
[3]	"Human Werner helicase interacting protein 1 (WRNIP1) functions as a novel modulator for DNA polymerase delta." Tsurimoto T., Shinozaki A., Yano M., Seki M., Enomoto T. Genes Cells 10:13-22(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WRNIP1.
[4]	"Functional roles of p12, the fourth subunit of human DNA polymerase delta." Li H., Xie B., Zhou Y., Rahmeh A., Trusa S., Zhang S., Gao Y., Lee E.Y., Lee M.Y. J. Biol. Chem. 281:14748-14755(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH POLD2; POLD1 AND PCNA, MUTAGENESIS OF ILE-7 AND 10-SER-TYR-11.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF179890 mRNA. Translation: AAG08966.1. AY928482 Genomic DNA. Translation: AAX09676.1.
IPI	IPI00028152.
RefSeq	NP_066996.3. NM_021173.4.
UniGene	Hs.523829.
3D structure databases
ProteinModelPortal	Q9HCU8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HCU8. 7 interactions.
STRING	9606.ENSP00000311368.
PTM databases
PhosphoSite	Q9HCU8.
Polymorphism databases
DMDM	18202958.
Proteomic databases
PaxDb	Q9HCU8.
PRIDE	Q9HCU8.
Protocols and materials databases
DNASU	57804.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312419; ENSP00000311368; ENSG00000175482.
GeneID	57804.
KEGG	hsa:57804.
UCSC	uc001okm.3. human.
Organism-specific databases
CTD	57804.
GeneCards	GC11M067119.
HGNC	HGNC:14106. POLD4.
MIM	611525. gene.
neXtProt	NX_Q9HCU8.
PharmGKB	PA33498.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG70894.
HOGENOM	HOG000031022.
HOVERGEN	HBG057838.
InParanoid	Q9HCU8.
KO	K03505.
OMA	WHRAEQM.
OrthoDB	EOG428235.
PhylomeDB	Q9HCU8.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_216. DNA Repair. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	Q9HCU8.
Bgee	Q9HCU8.
CleanEx	HS_POLD4.
Genevestigator	Q9HCU8.
GermOnline	ENSG00000175482. Homo sapiens.
Family and domain databases
InterPro	IPR007218. DNA_pol_delta_4. [Graphical view]
Pfam	PF04081. DNA_pol_delta_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL2168.
GenomeRNAi	57804.
NextBio	64752.
SOURCE	Search...

Entry information

Entry name

DPOD4_HUMAN

Accession

Primary (citable) accession number: Q9HCU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.