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Protein

Prolactin regulatory element-binding protein

Gene

PREB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER.By similarity
Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processER-Golgi transport, Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-381038. XBP1(S) activates chaperone genes.
R-HSA-5694530. Cargo concentration in the ER.
SignaLinkiQ9HCU5.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin regulatory element-binding protein
Alternative name(s):
Mammalian guanine nucleotide exchange factor mSec12
Gene namesi
Name:PREB
Synonyms:SEC12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9356. PREB.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity
  • Nucleus By similarity

  • Note: Concentrates at endoplasmic reticulum exit sites.1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 388CytoplasmicSequence analysisAdd BLAST388
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 417LumenalSequence analysis8

GO - Cellular componenti

  • endoplasmic reticulum exit site Source: UniProtKB
  • endoplasmic reticulum membrane Source: Reactome
  • Golgi membrane Source: GOC
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000138073.
PharmGKBiPA33727.

Polymorphism and mutation databases

BioMutaiPREB.
DMDMi55977881.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511541 – 417Prolactin regulatory element-binding proteinAdd BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Nitrated tyrosineBy similarity1

Keywords - PTMi

Nitration

Proteomic databases

EPDiQ9HCU5.
MaxQBiQ9HCU5.
PaxDbiQ9HCU5.
PeptideAtlasiQ9HCU5.
PRIDEiQ9HCU5.

PTM databases

iPTMnetiQ9HCU5.
PhosphoSitePlusiQ9HCU5.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

BgeeiENSG00000138073.
CleanExiHS_PREB.
ExpressionAtlasiQ9HCU5. baseline and differential.
GenevisibleiQ9HCU5. HS.

Organism-specific databases

HPAiHPA013582.
HPA014133.

Interactioni

Subunit structurei

Interacts with SAR1B (GDP-bound form) (By similarity). Interacts with CTAGE5; recruits PREB to endoplasmic reticulum exit sites (PubMed:25202031, PubMed:27170179).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115419. 20 interactors.
IntActiQ9HCU5. 20 interactors.
MINTiMINT-4539832.
STRINGi9606.ENSP00000260643.

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 22Combined sources7
Turni23 – 26Combined sources4
Beta strandi27 – 33Combined sources7
Beta strandi38 – 40Combined sources3
Beta strandi42 – 52Combined sources11
Beta strandi57 – 66Combined sources10
Beta strandi72 – 78Combined sources7
Beta strandi81 – 86Combined sources6
Beta strandi89 – 97Combined sources9
Beta strandi139 – 148Combined sources10
Beta strandi152 – 154Combined sources3
Beta strandi157 – 162Combined sources6
Beta strandi166 – 173Combined sources8
Beta strandi177 – 182Combined sources6
Turni183 – 185Combined sources3
Beta strandi188 – 193Combined sources6
Beta strandi199 – 204Combined sources6
Helixi206 – 208Combined sources3
Beta strandi210 – 214Combined sources5
Beta strandi219 – 223Combined sources5
Beta strandi226 – 231Combined sources6
Beta strandi245 – 254Combined sources10
Beta strandi262 – 272Combined sources11
Beta strandi281 – 286Combined sources6
Turni287 – 289Combined sources3
Beta strandi292 – 297Combined sources6
Beta strandi303 – 308Combined sources6
Beta strandi312 – 319Combined sources8
Beta strandi324 – 328Combined sources5
Turni329 – 332Combined sources4
Beta strandi333 – 338Combined sources6
Beta strandi341 – 344Combined sources4
Beta strandi346 – 351Combined sources6
Helixi358 – 362Combined sources5
Beta strandi368 – 373Combined sources6
Beta strandi376 – 382Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5TF2X-ray2.80A1-389[»]
ProteinModelPortaliQ9HCU5.
SMRiQ9HCU5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 50WD 1Add BLAST47
Repeati58 – 97WD 2Add BLAST40
Repeati127 – 183WD 3Add BLAST57
Repeati187 – 224WD 4Add BLAST38
Repeati230 – 287WD 5Add BLAST58
Repeati291 – 329WD 6Add BLAST39
Repeati334 – 385WD 7Add BLAST52

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

eggNOGiKOG0771. Eukaryota.
ENOG410XRQK. LUCA.
GeneTreeiENSGT00390000018031.
HOGENOMiHOG000045302.
HOVERGENiHBG002027.
InParanoidiQ9HCU5.
KOiK14003.
OMAiAHSMFVT.
OrthoDBiEOG091G06TN.
PhylomeDBiQ9HCU5.
TreeFamiTF314383.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF00400. WD40. 1 hit.
SMARTiView protein in SMART
SM00320. WD40. 4 hits.
SUPFAMiSSF50998. SSF50998. 2 hits.
PROSITEiView protein in PROSITE
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HCU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRRAPELY RAPFPLYALQ VDPSTGLLIA AGGGGAAKTG IKNGVHFLQL
60 70 80 90 100
ELINGRLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAHC QLLRFQAHQQ
110 120 130 140 150
QGNKAEKAGS KEQGPRQRKG AAPAEKKCGA ETQHEGLELR VENLQAVQTD
160 170 180 190 200
FSSDPLQKVV CFNHDNTLLA TGGTDGYVRV WKVPSLEKVL EFKAHEGEIE
210 220 230 240 250
DLALGPDGKL VTVGRDLKAS VWQKDQLVTQ LHWQENGPTF SSTPYRYQAC
260 270 280 290 300
RFGQVPDQPA GLRLFTVQIP HKRLRQPPPC YLTAWDGSNF LPLRTKSCGH
310 320 330 340 350
EVVSCLDVSE SGTFLGLGTV TGSVAIYIAF SLQCLYYVRE AHGIVVTDVA
360 370 380 390 400
FLPEKGRGPE LLGSHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG
410
LIIVTILLLQ SAFPGFL
Length:417
Mass (Da):45,468
Last modified:November 23, 2004 - v2
Checksum:i7FD490A641202D19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19L → F in AAG01692 (PubMed:10920239).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226684 mRNA. Translation: AAG01692.1.
AF203687 mRNA. Translation: AAF19192.1.
AF227166 Genomic DNA. Translation: AAF74572.1.
AK023064 mRNA. Translation: BAB14385.1.
AC013403 Genomic DNA. Translation: AAX93170.1.
CH471053 Genomic DNA. Translation: EAX00627.1.
BC002765 mRNA. Translation: AAH02765.1.
BC012890 mRNA. Translation: AAH12890.1.
BC016472 mRNA. Translation: AAH16472.1.
BC041032 mRNA. Translation: AAH41032.1.
CCDSiCCDS1738.1.
RefSeqiNP_001317414.1. NM_001330485.1.
NP_001317415.1. NM_001330486.1.
NP_037520.1. NM_013388.5.
UniGeneiHs.279784.

Genome annotation databases

EnsembliENST00000260643; ENSP00000260643; ENSG00000138073.
GeneIDi10113.
KEGGihsa:10113.
UCSCiuc002rix.3. human.

Similar proteinsi

Entry informationi

Entry nameiPREB_HUMAN
AccessioniPrimary (citable) accession number: Q9HCU5
Secondary accession number(s): Q53SZ8, Q9UH94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: November 23, 2004
Last modified: August 30, 2017
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references