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Q9HCU5

- PREB_HUMAN

UniProt

Q9HCU5 - PREB_HUMAN

Protein

Prolactin regulatory element-binding protein

Gene

PREB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription By similarity. Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. activation of signaling protein activity involved in unfolded protein response Source: Reactome
    2. cellular protein metabolic process Source: Reactome
    3. COPII vesicle coating Source: Reactome
    4. endoplasmic reticulum unfolded protein response Source: Reactome
    5. ER to Golgi vesicle-mediated transport Source: Reactome
    6. membrane organization Source: Reactome
    7. post-translational protein modification Source: Reactome
    8. protein N-linked glycosylation via asparagine Source: Reactome
    9. protein transport Source: UniProtKB-KW
    10. regulation of transcription, DNA-templated Source: ProtInc
    11. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transcription, Transcription regulation, Transport

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_12507. COPII (Coat Protein 2) Mediated Vesicle Transport.
    REACT_18273. XBP1(S) activates chaperone genes.
    SignaLinkiQ9HCU5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prolactin regulatory element-binding protein
    Alternative name(s):
    Mammalian guanine nucleotide exchange factor mSec12
    Gene namesi
    Name:PREB
    Synonyms:SEC12
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:9356. PREB.

    Subcellular locationi

    Nucleus By similarity. Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome
    2. Golgi membrane Source: GOC
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: UniProtKB
    5. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33727.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 417417Prolactin regulatory element-binding proteinPRO_0000051154Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei10 – 101Nitrated tyrosineBy similarity

    Keywords - PTMi

    Nitration

    Proteomic databases

    MaxQBiQ9HCU5.
    PaxDbiQ9HCU5.
    PeptideAtlasiQ9HCU5.
    PRIDEiQ9HCU5.

    PTM databases

    PhosphoSiteiQ9HCU5.

    Expressioni

    Tissue specificityi

    Ubiquitous.2 Publications

    Gene expression databases

    ArrayExpressiQ9HCU5.
    BgeeiQ9HCU5.
    CleanExiHS_PREB.
    GenevestigatoriQ9HCU5.

    Organism-specific databases

    HPAiHPA013582.
    HPA014133.

    Interactioni

    Subunit structurei

    Binds SARA2.

    Protein-protein interaction databases

    BioGridi115419. 7 interactions.
    IntActiQ9HCU5. 5 interactions.
    MINTiMINT-4539832.
    STRINGi9606.ENSP00000260643.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HCU5.
    SMRiQ9HCU5. Positions 152-222, 290-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 388388CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini410 – 4178LumenalSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei389 – 40921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati152 – 19140WD 1Add
    BLAST
    Repeati194 – 23239WD 2Add
    BLAST
    Repeati298 – 33740WD 3Add
    BLAST

    Sequence similaritiesi

    Contains 3 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix, WD repeat

    Phylogenomic databases

    eggNOGiNOG309181.
    HOGENOMiHOG000045302.
    HOVERGENiHBG002027.
    InParanoidiQ9HCU5.
    KOiK14003.
    OMAiAAGQDAH.
    OrthoDBiEOG7XM305.
    PhylomeDBiQ9HCU5.
    TreeFamiTF314383.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR011047. Quinonprotein_ADH-like_supfam.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 2 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 4 hits.
    [Graphical view]
    SUPFAMiSSF50998. SSF50998. 2 hits.
    PROSITEiPS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HCU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRRRAPELY RAPFPLYALQ VDPSTGLLIA AGGGGAAKTG IKNGVHFLQL    50
    ELINGRLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAHC QLLRFQAHQQ 100
    QGNKAEKAGS KEQGPRQRKG AAPAEKKCGA ETQHEGLELR VENLQAVQTD 150
    FSSDPLQKVV CFNHDNTLLA TGGTDGYVRV WKVPSLEKVL EFKAHEGEIE 200
    DLALGPDGKL VTVGRDLKAS VWQKDQLVTQ LHWQENGPTF SSTPYRYQAC 250
    RFGQVPDQPA GLRLFTVQIP HKRLRQPPPC YLTAWDGSNF LPLRTKSCGH 300
    EVVSCLDVSE SGTFLGLGTV TGSVAIYIAF SLQCLYYVRE AHGIVVTDVA 350
    FLPEKGRGPE LLGSHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG 400
    LIIVTILLLQ SAFPGFL 417
    Length:417
    Mass (Da):45,468
    Last modified:November 23, 2004 - v2
    Checksum:i7FD490A641202D19
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191L → F in AAG01692. (PubMed:10920239)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF226684 mRNA. Translation: AAG01692.1.
    AF203687 mRNA. Translation: AAF19192.1.
    AF227166 Genomic DNA. Translation: AAF74572.1.
    AK023064 mRNA. Translation: BAB14385.1.
    AC013403 Genomic DNA. Translation: AAX93170.1.
    CH471053 Genomic DNA. Translation: EAX00627.1.
    BC002765 mRNA. Translation: AAH02765.1.
    BC012890 mRNA. Translation: AAH12890.1.
    BC016472 mRNA. Translation: AAH16472.1.
    BC041032 mRNA. Translation: AAH41032.1.
    CCDSiCCDS1738.1.
    RefSeqiNP_037520.1. NM_013388.4.
    UniGeneiHs.279784.

    Genome annotation databases

    EnsembliENST00000260643; ENSP00000260643; ENSG00000138073.
    GeneIDi10113.
    KEGGihsa:10113.
    UCSCiuc002rix.1. human.

    Polymorphism databases

    DMDMi55977881.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF226684 mRNA. Translation: AAG01692.1 .
    AF203687 mRNA. Translation: AAF19192.1 .
    AF227166 Genomic DNA. Translation: AAF74572.1 .
    AK023064 mRNA. Translation: BAB14385.1 .
    AC013403 Genomic DNA. Translation: AAX93170.1 .
    CH471053 Genomic DNA. Translation: EAX00627.1 .
    BC002765 mRNA. Translation: AAH02765.1 .
    BC012890 mRNA. Translation: AAH12890.1 .
    BC016472 mRNA. Translation: AAH16472.1 .
    BC041032 mRNA. Translation: AAH41032.1 .
    CCDSi CCDS1738.1.
    RefSeqi NP_037520.1. NM_013388.4.
    UniGenei Hs.279784.

    3D structure databases

    ProteinModelPortali Q9HCU5.
    SMRi Q9HCU5. Positions 152-222, 290-356.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115419. 7 interactions.
    IntActi Q9HCU5. 5 interactions.
    MINTi MINT-4539832.
    STRINGi 9606.ENSP00000260643.

    PTM databases

    PhosphoSitei Q9HCU5.

    Polymorphism databases

    DMDMi 55977881.

    Proteomic databases

    MaxQBi Q9HCU5.
    PaxDbi Q9HCU5.
    PeptideAtlasi Q9HCU5.
    PRIDEi Q9HCU5.

    Protocols and materials databases

    DNASUi 10113.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000260643 ; ENSP00000260643 ; ENSG00000138073 .
    GeneIDi 10113.
    KEGGi hsa:10113.
    UCSCi uc002rix.1. human.

    Organism-specific databases

    CTDi 10113.
    GeneCardsi GC02M027353.
    HGNCi HGNC:9356. PREB.
    HPAi HPA013582.
    HPA014133.
    MIMi 606395. gene.
    neXtProti NX_Q9HCU5.
    PharmGKBi PA33727.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG309181.
    HOGENOMi HOG000045302.
    HOVERGENi HBG002027.
    InParanoidi Q9HCU5.
    KOi K14003.
    OMAi AAGQDAH.
    OrthoDBi EOG7XM305.
    PhylomeDBi Q9HCU5.
    TreeFami TF314383.

    Enzyme and pathway databases

    Reactomei REACT_12507. COPII (Coat Protein 2) Mediated Vesicle Transport.
    REACT_18273. XBP1(S) activates chaperone genes.
    SignaLinki Q9HCU5.

    Miscellaneous databases

    GeneWikii PREB.
    GenomeRNAii 10113.
    NextBioi 38259.
    PROi Q9HCU5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9HCU5.
    Bgeei Q9HCU5.
    CleanExi HS_PREB.
    Genevestigatori Q9HCU5.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR011047. Quinonprotein_ADH-like_supfam.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 2 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 4 hits.
    [Graphical view ]
    SUPFAMi SSF50998. SSF50998. 2 hits.
    PROSITEi PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of human PREB; a gene that maps to a genomic region associated with trisomy 2p syndrome."
      Taylor Clelland C.L., Levy B., McKie J.M., Duncan A.M.V., Hirschhorn K., Bancroft C.
      Mamm. Genome 11:675-681(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
    2. "The human and mouse homologues of rat prolactin regulatory element binding protein."
      Edgar A.J., Polak J.M.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "The gene structure and expression of human ABHD1: overlapping polyadenylation signal sequence with Sec12."
      Edgar A.J.
      BMC Genomics 4:18-18(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain, Lung and Uterus.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPREB_HUMAN
    AccessioniPrimary (citable) accession number: Q9HCU5
    Secondary accession number(s): Q53SZ8, Q9UH94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 24, 2001
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3