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Protein

Prolactin regulatory element-binding protein

Gene

PREB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER.By similarity
Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-381038. XBP1(S) activates chaperone genes.
R-HSA-5694530. Cargo concentration in the ER.
SignaLinkiQ9HCU5.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin regulatory element-binding protein
Alternative name(s):
Mammalian guanine nucleotide exchange factor mSec12
Gene namesi
Name:PREB
Synonyms:SEC12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:9356. PREB.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity
  • Nucleus By similarity

  • Note: Concentrates at endoplasmic reticulum exit sites.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 388388CytoplasmicSequence analysisAdd
BLAST
Transmembranei389 – 40921HelicalSequence analysisAdd
BLAST
Topological domaini410 – 4178LumenalSequence analysis

GO - Cellular componenti

  • endoplasmic reticulum exit site Source: UniProtKB
  • endoplasmic reticulum membrane Source: Reactome
  • Golgi membrane Source: GOC
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33727.

Polymorphism and mutation databases

BioMutaiPREB.
DMDMi55977881.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Prolactin regulatory element-binding proteinPRO_0000051154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Nitrated tyrosineBy similarity

Keywords - PTMi

Nitration

Proteomic databases

EPDiQ9HCU5.
MaxQBiQ9HCU5.
PaxDbiQ9HCU5.
PeptideAtlasiQ9HCU5.
PRIDEiQ9HCU5.

PTM databases

iPTMnetiQ9HCU5.
PhosphoSiteiQ9HCU5.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

BgeeiENSG00000138073.
CleanExiHS_PREB.
ExpressionAtlasiQ9HCU5. baseline and differential.
GenevisibleiQ9HCU5. HS.

Organism-specific databases

HPAiHPA013582.
HPA014133.

Interactioni

Subunit structurei

Interacts with SAR1B (GDP-bound form) (By similarity). Interacts with CTAGE5; recruits PREB to endoplasmic reticulum exit sites (PubMed:25202031, PubMed:27170179).By similarity2 Publications

Protein-protein interaction databases

BioGridi115419. 16 interactions.
IntActiQ9HCU5. 19 interactions.
MINTiMINT-4539832.
STRINGi9606.ENSP00000260643.

Structurei

3D structure databases

ProteinModelPortaliQ9HCU5.
SMRiQ9HCU5. Positions 152-222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati4 – 5047WD 1Add
BLAST
Repeati58 – 9740WD 2Add
BLAST
Repeati127 – 18357WD 3Add
BLAST
Repeati187 – 22438WD 4Add
BLAST
Repeati230 – 28758WD 5Add
BLAST
Repeati291 – 32939WD 6Add
BLAST
Repeati334 – 38552WD 7Add
BLAST

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

eggNOGiKOG0771. Eukaryota.
ENOG410XRQK. LUCA.
GeneTreeiENSGT00390000018031.
HOGENOMiHOG000045302.
HOVERGENiHBG002027.
InParanoidiQ9HCU5.
KOiK14003.
OMAiAHSMFVT.
OrthoDBiEOG091G06TN.
PhylomeDBiQ9HCU5.
TreeFamiTF314383.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HCU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRRAPELY RAPFPLYALQ VDPSTGLLIA AGGGGAAKTG IKNGVHFLQL
60 70 80 90 100
ELINGRLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAHC QLLRFQAHQQ
110 120 130 140 150
QGNKAEKAGS KEQGPRQRKG AAPAEKKCGA ETQHEGLELR VENLQAVQTD
160 170 180 190 200
FSSDPLQKVV CFNHDNTLLA TGGTDGYVRV WKVPSLEKVL EFKAHEGEIE
210 220 230 240 250
DLALGPDGKL VTVGRDLKAS VWQKDQLVTQ LHWQENGPTF SSTPYRYQAC
260 270 280 290 300
RFGQVPDQPA GLRLFTVQIP HKRLRQPPPC YLTAWDGSNF LPLRTKSCGH
310 320 330 340 350
EVVSCLDVSE SGTFLGLGTV TGSVAIYIAF SLQCLYYVRE AHGIVVTDVA
360 370 380 390 400
FLPEKGRGPE LLGSHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG
410
LIIVTILLLQ SAFPGFL
Length:417
Mass (Da):45,468
Last modified:November 23, 2004 - v2
Checksum:i7FD490A641202D19
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191L → F in AAG01692 (PubMed:10920239).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226684 mRNA. Translation: AAG01692.1.
AF203687 mRNA. Translation: AAF19192.1.
AF227166 Genomic DNA. Translation: AAF74572.1.
AK023064 mRNA. Translation: BAB14385.1.
AC013403 Genomic DNA. Translation: AAX93170.1.
CH471053 Genomic DNA. Translation: EAX00627.1.
BC002765 mRNA. Translation: AAH02765.1.
BC012890 mRNA. Translation: AAH12890.1.
BC016472 mRNA. Translation: AAH16472.1.
BC041032 mRNA. Translation: AAH41032.1.
CCDSiCCDS1738.1.
RefSeqiNP_037520.1. NM_013388.4.
UniGeneiHs.279784.

Genome annotation databases

EnsembliENST00000260643; ENSP00000260643; ENSG00000138073.
GeneIDi10113.
KEGGihsa:10113.
UCSCiuc002rix.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226684 mRNA. Translation: AAG01692.1.
AF203687 mRNA. Translation: AAF19192.1.
AF227166 Genomic DNA. Translation: AAF74572.1.
AK023064 mRNA. Translation: BAB14385.1.
AC013403 Genomic DNA. Translation: AAX93170.1.
CH471053 Genomic DNA. Translation: EAX00627.1.
BC002765 mRNA. Translation: AAH02765.1.
BC012890 mRNA. Translation: AAH12890.1.
BC016472 mRNA. Translation: AAH16472.1.
BC041032 mRNA. Translation: AAH41032.1.
CCDSiCCDS1738.1.
RefSeqiNP_037520.1. NM_013388.4.
UniGeneiHs.279784.

3D structure databases

ProteinModelPortaliQ9HCU5.
SMRiQ9HCU5. Positions 152-222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115419. 16 interactions.
IntActiQ9HCU5. 19 interactions.
MINTiMINT-4539832.
STRINGi9606.ENSP00000260643.

PTM databases

iPTMnetiQ9HCU5.
PhosphoSiteiQ9HCU5.

Polymorphism and mutation databases

BioMutaiPREB.
DMDMi55977881.

Proteomic databases

EPDiQ9HCU5.
MaxQBiQ9HCU5.
PaxDbiQ9HCU5.
PeptideAtlasiQ9HCU5.
PRIDEiQ9HCU5.

Protocols and materials databases

DNASUi10113.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260643; ENSP00000260643; ENSG00000138073.
GeneIDi10113.
KEGGihsa:10113.
UCSCiuc002rix.3. human.

Organism-specific databases

CTDi10113.
GeneCardsiPREB.
HGNCiHGNC:9356. PREB.
HPAiHPA013582.
HPA014133.
MIMi606395. gene.
neXtProtiNX_Q9HCU5.
PharmGKBiPA33727.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0771. Eukaryota.
ENOG410XRQK. LUCA.
GeneTreeiENSGT00390000018031.
HOGENOMiHOG000045302.
HOVERGENiHBG002027.
InParanoidiQ9HCU5.
KOiK14003.
OMAiAHSMFVT.
OrthoDBiEOG091G06TN.
PhylomeDBiQ9HCU5.
TreeFamiTF314383.

Enzyme and pathway databases

ReactomeiR-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-381038. XBP1(S) activates chaperone genes.
R-HSA-5694530. Cargo concentration in the ER.
SignaLinkiQ9HCU5.

Miscellaneous databases

ChiTaRSiPREB. human.
GeneWikiiPREB.
GenomeRNAii10113.
PROiQ9HCU5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138073.
CleanExiHS_PREB.
ExpressionAtlasiQ9HCU5. baseline and differential.
GenevisibleiQ9HCU5. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPREB_HUMAN
AccessioniPrimary (citable) accession number: Q9HCU5
Secondary accession number(s): Q53SZ8, Q9UH94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.