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Q9HCU5 (PREB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolactin regulatory element-binding protein
Alternative name(s):
Mammalian guanine nucleotide exchange factor mSec12
Gene names
Name:PREB
Synonyms:SEC12
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription By similarity. Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.

Subunit structure

Binds SARA2.

Subcellular location

Nucleus By similarity. Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.

Tissue specificity

Ubiquitous. Ref.1 Ref.3

Sequence similarities

Contains 3 WD repeats.

Ontologies

Keywords
   Biological processER-Golgi transport
Protein transport
Transcription
Transcription regulation
Transport
   Cellular componentEndoplasmic reticulum
Membrane
Nucleus
   DomainRepeat
Transmembrane
Transmembrane helix
WD repeat
   LigandDNA-binding
   Molecular functionActivator
   PTMNitration
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processCOPII vesicle coating

Traceable author statement. Source: Reactome

ER to Golgi vesicle-mediated transport

Traceable author statement. Source: Reactome

activation of signaling protein activity involved in unfolded protein response

Traceable author statement. Source: Reactome

cellular protein metabolic process

Traceable author statement. Source: Reactome

endoplasmic reticulum unfolded protein response

Traceable author statement. Source: Reactome

membrane organization

Traceable author statement. Source: Reactome

post-translational protein modification

Traceable author statement. Source: Reactome

protein N-linked glycosylation via asparagine

Traceable author statement. Source: Reactome

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of transcription, DNA-templated

Traceable author statement PubMed 10194769. Source: ProtInc

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi membrane

Traceable author statement. Source: GOC

endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nucleus

Traceable author statement PubMed 10194769. Source: ProtInc

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Prolactin regulatory element-binding protein
PRO_0000051154

Regions

Topological domain1 – 388388Cytoplasmic Potential
Transmembrane389 – 40921Helical; Potential
Topological domain410 – 4178Lumenal Potential
Repeat152 – 19140WD 1
Repeat194 – 23239WD 2
Repeat298 – 33740WD 3

Amino acid modifications

Modified residue101Nitrated tyrosine By similarity

Experimental info

Sequence conflict191L → F in AAG01692. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9HCU5 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: 7FD490A641202D19

FASTA41745,468
        10         20         30         40         50         60 
MGRRRAPELY RAPFPLYALQ VDPSTGLLIA AGGGGAAKTG IKNGVHFLQL ELINGRLSAS 

        70         80         90        100        110        120 
LLHSHDTETR ATMNLALAGD ILAAGQDAHC QLLRFQAHQQ QGNKAEKAGS KEQGPRQRKG 

       130        140        150        160        170        180 
AAPAEKKCGA ETQHEGLELR VENLQAVQTD FSSDPLQKVV CFNHDNTLLA TGGTDGYVRV 

       190        200        210        220        230        240 
WKVPSLEKVL EFKAHEGEIE DLALGPDGKL VTVGRDLKAS VWQKDQLVTQ LHWQENGPTF 

       250        260        270        280        290        300 
SSTPYRYQAC RFGQVPDQPA GLRLFTVQIP HKRLRQPPPC YLTAWDGSNF LPLRTKSCGH 

       310        320        330        340        350        360 
EVVSCLDVSE SGTFLGLGTV TGSVAIYIAF SLQCLYYVRE AHGIVVTDVA FLPEKGRGPE 

       370        380        390        400        410 
LLGSHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG LIIVTILLLQ SAFPGFL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of human PREB; a gene that maps to a genomic region associated with trisomy 2p syndrome."
Taylor Clelland C.L., Levy B., McKie J.M., Duncan A.M.V., Hirschhorn K., Bancroft C.
Mamm. Genome 11:675-681(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
[2]"The human and mouse homologues of rat prolactin regulatory element binding protein."
Edgar A.J., Polak J.M.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[3]"The gene structure and expression of human ABHD1: overlapping polyadenylation signal sequence with Sec12."
Edgar A.J.
BMC Genomics 4:18-18(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain, Lung and Uterus.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF226684 mRNA. Translation: AAG01692.1.
AF203687 mRNA. Translation: AAF19192.1.
AF227166 Genomic DNA. Translation: AAF74572.1.
AK023064 mRNA. Translation: BAB14385.1.
AC013403 Genomic DNA. Translation: AAX93170.1.
CH471053 Genomic DNA. Translation: EAX00627.1.
BC002765 mRNA. Translation: AAH02765.1.
BC012890 mRNA. Translation: AAH12890.1.
BC016472 mRNA. Translation: AAH16472.1.
BC041032 mRNA. Translation: AAH41032.1.
RefSeqNP_037520.1. NM_013388.4.
UniGeneHs.279784.

3D structure databases

ProteinModelPortalQ9HCU5.
SMRQ9HCU5. Positions 152-222.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115419. 6 interactions.
IntActQ9HCU5. 3 interactions.
MINTMINT-4539832.
STRING9606.ENSP00000260643.

PTM databases

PhosphoSiteQ9HCU5.

Polymorphism databases

DMDM55977881.

Proteomic databases

PaxDbQ9HCU5.
PeptideAtlasQ9HCU5.
PRIDEQ9HCU5.

Protocols and materials databases

DNASU10113.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000260643; ENSP00000260643; ENSG00000138073.
GeneID10113.
KEGGhsa:10113.
UCSCuc002rix.1. human.

Organism-specific databases

CTD10113.
GeneCardsGC02M027353.
HGNCHGNC:9356. PREB.
HPAHPA013582.
HPA014133.
MIM606395. gene.
neXtProtNX_Q9HCU5.
PharmGKBPA33727.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG309181.
HOGENOMHOG000045302.
HOVERGENHBG002027.
InParanoidQ9HCU5.
KOK14003.
OMAAAGQDAH.
OrthoDBEOG7XM305.
PhylomeDBQ9HCU5.
TreeFamTF314383.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.
REACT_17015. Metabolism of proteins.
SignaLinkQ9HCU5.

Gene expression databases

ArrayExpressQ9HCU5.
BgeeQ9HCU5.
CleanExHS_PREB.
GenevestigatorQ9HCU5.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 2 hits.
[Graphical view]
SMARTSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMSSF50998. SSF50998. 2 hits.
PROSITEPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPREB.
GenomeRNAi10113.
NextBio38259.
PROQ9HCU5.
SOURCESearch...

Entry information

Entry namePREB_HUMAN
AccessionPrimary (citable) accession number: Q9HCU5
Secondary accession number(s): Q53SZ8, Q9UH94
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM