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Q9HCS2

- CP4FC_HUMAN

UniProt

Q9HCS2 - CP4FC_HUMAN

Protein

Cytochrome P450 4F12

Gene

CYP4F12

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. Catalyzes the hydroxylation of the antihistamine ebastine.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi468 – 4681Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. alkane 1-monooxygenase activity Source: UniProtKB
    2. arachidonic acid epoxygenase activity Source: UniProtKB
    3. aromatase activity Source: UniProtKB-EC
    4. heme binding Source: InterPro
    5. iron ion binding Source: InterPro
    6. leukotriene-B4 20-monooxygenase activity Source: UniProtKB
    7. vitamin-K-epoxide reductase (warfarin-sensitive) activity Source: UniProtKB

    GO - Biological processi

    1. arachidonic acid metabolic process Source: UniProtKB
    2. drug metabolic process Source: UniProtKB
    3. epoxygenase P450 pathway Source: UniProtKB
    4. leukotriene B4 catabolic process Source: UniProtKB
    5. long-chain fatty acid metabolic process Source: UniProtKB
    6. negative regulation of blood coagulation Source: UniProtKB
    7. oxidation-reduction process Source: UniProtKB
    8. pressure natriuresis Source: UniProtKB
    9. renal water homeostasis Source: UniProtKB
    10. small molecule metabolic process Source: Reactome
    11. sodium ion homeostasis Source: UniProtKB
    12. very long-chain fatty acid metabolic process Source: UniProtKB
    13. vitamin E metabolic process Source: UniProtKB
    14. vitamin K biosynthetic process Source: UniProtKB
    15. xenobiotic metabolic process Source: Reactome

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_13645. Eicosanoids.
    REACT_13814. Fatty acids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 4F12 (EC:1.14.14.1)
    Alternative name(s):
    CYPIVF12
    Gene namesi
    Name:CYP4F12
    ORF Names:UNQ568/PRO1129
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:18857. CYP4F12.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. endoplasmic reticulum membrane Source: Reactome
    4. integral component of membrane Source: UniProtKB-KW
    5. intracellular membrane-bounded organelle Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38717.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Cytochrome P450 4F12PRO_0000051857Add
    BLAST

    Proteomic databases

    MaxQBiQ9HCS2.
    PaxDbiQ9HCS2.
    PRIDEiQ9HCS2.

    PTM databases

    PhosphoSiteiQ9HCS2.

    Expressioni

    Tissue specificityi

    Expressed in small intestine, liver, colon and heart.2 Publications

    Gene expression databases

    ArrayExpressiQ9HCS2.
    BgeeiQ9HCS2.
    CleanExiHS_CYP4F12.
    GenevestigatoriQ9HCS2.

    Interactioni

    Protein-protein interaction databases

    BioGridi122449. 3 interactions.
    IntActiQ9HCS2. 3 interactions.
    STRINGi9606.ENSP00000321821.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HCS2.
    SMRiQ9HCS2. Positions 97-524.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 3921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei87 – 10721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000233833.
    HOVERGENiHBG000182.
    InParanoidiQ9HCS2.
    KOiK17730.
    OMAiIICVINI.
    OrthoDBiEOG7CNZFK.
    PhylomeDBiQ9HCS2.
    TreeFamiTF105088.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9HCS2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLLSLPWLG LRPVATSPWL LLLLVVGSWL LARILAWTYA FYNNCRRLQC    50
    FPQPPKRNWF WGHLGLITPT EEGLKNSTQM SATYSQGFTI WLGPIIPFIV 100
    LCHPDTIRSI TNASAAIAPK DNLFIRFLKP WLGEGILLSG GDKWSRHRRM 150
    LTPAFHFNIL KSYITIFNKS ANIMLDKWQH LASEGSSCLD MFEHISLMTL 200
    DSLQKCIFSF DSHCQERPSE YIATILELSA LVEKRSQHIL QHMDFLYYLS 250
    HDGRRFHRAC RLVHDFTDAV IRERRRTLPT QGIDDFFKDK AKSKTLDFID 300
    VLLLSKDEDG KALSDEDIRA EADTFMFGGH DTTASGLSWV LYNLARHPEY 350
    QERCRQEVQE LLKDRDPKEI EWDDLAQLPF LTMCVKESLR LHPPAPFISR 400
    CCTQDIVLPD GRVIPKGITC LIDIIGVHHN PTVWPDPEVY DPFRFDPENS 450
    KGRSPLAFIP FSAGPRNCIG QAFAMAEMKV VLALMLLHFR FLPDHTEPRR 500
    KLELIMRAEG GLWLRVEPLN VSLQ 524
    Length:524
    Mass (Da):60,270
    Last modified:November 30, 2010 - v2
    Checksum:iF29C29BA8DB880FE
    GO
    Isoform 2 (identifier: Q9HCS2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         90-102: IWLGPIIPFIVLC → LPLHPRIISSSGS
         103-524: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:102
    Mass (Da):11,572
    Checksum:i356BE81361CDE5A9
    GO

    Sequence cautioni

    The sequence EAW84492.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti13 – 131P → L.1 Publication
    Corresponds to variant rs16995376 [ dbSNP | Ensembl ].
    VAR_013244
    Natural varianti16 – 161T → M.6 Publications
    Corresponds to variant rs16995378 [ dbSNP | Ensembl ].
    VAR_048459
    Natural varianti76 – 761N → D.6 Publications
    Corresponds to variant rs609636 [ dbSNP | Ensembl ].
    VAR_013245
    Natural varianti90 – 901I → V.4 Publications
    Corresponds to variant rs609290 [ dbSNP | Ensembl ].
    VAR_013246
    Natural varianti188 – 1881C → R.4 Publications
    Corresponds to variant rs2285888 [ dbSNP | Ensembl ].
    VAR_013247
    Natural varianti522 – 5221S → G.4 Publications
    Corresponds to variant rs593818 [ dbSNP | Ensembl ].
    VAR_048460

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei90 – 10213IWLGP…FIVLC → LPLHPRIISSSGS in isoform 2. 2 PublicationsVSP_055581Add
    BLAST
    Alternative sequencei103 – 524422Missing in isoform 2. 2 PublicationsVSP_055582Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY008841 mRNA. Translation: AAG33247.1.
    AB035130 mRNA. Translation: BAB18269.1.
    AB035131 mRNA. Translation: BAB18270.1.
    AY358977 mRNA. Translation: AAQ89336.1.
    AK075435 mRNA. Translation: BAG52137.1.
    AL832171 mRNA. Translation: CAI46131.1.
    AC004523 Genomic DNA. Translation: AAC11543.1.
    AC122702 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84492.1. Different initiation.
    CH471106 Genomic DNA. Translation: EAW84495.1.
    CCDSiCCDS42517.1.
    PIRiJC7594.
    JC7598.
    RefSeqiNP_076433.3. NM_023944.3.
    UniGeneiHs.131459.

    Genome annotation databases

    EnsembliENST00000324632; ENSP00000321821; ENSG00000186204. [Q9HCS2-1]
    ENST00000517734; ENSP00000430849; ENSG00000186204. [Q9HCS2-2]
    ENST00000548435; ENSP00000449703; ENSG00000186204. [Q9HCS2-2]
    ENST00000550308; ENSP00000448998; ENSG00000186204. [Q9HCS2-1]
    GeneIDi66002.
    KEGGihsa:66002.
    UCSCiuc002nbl.3. human.

    Polymorphism databases

    DMDMi313104094.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY008841 mRNA. Translation: AAG33247.1 .
    AB035130 mRNA. Translation: BAB18269.1 .
    AB035131 mRNA. Translation: BAB18270.1 .
    AY358977 mRNA. Translation: AAQ89336.1 .
    AK075435 mRNA. Translation: BAG52137.1 .
    AL832171 mRNA. Translation: CAI46131.1 .
    AC004523 Genomic DNA. Translation: AAC11543.1 .
    AC122702 Genomic DNA. No translation available.
    CH471106 Genomic DNA. Translation: EAW84492.1 . Different initiation.
    CH471106 Genomic DNA. Translation: EAW84495.1 .
    CCDSi CCDS42517.1.
    PIRi JC7594.
    JC7598.
    RefSeqi NP_076433.3. NM_023944.3.
    UniGenei Hs.131459.

    3D structure databases

    ProteinModelPortali Q9HCS2.
    SMRi Q9HCS2. Positions 97-524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122449. 3 interactions.
    IntActi Q9HCS2. 3 interactions.
    STRINGi 9606.ENSP00000321821.

    PTM databases

    PhosphoSitei Q9HCS2.

    Polymorphism databases

    DMDMi 313104094.

    Proteomic databases

    MaxQBi Q9HCS2.
    PaxDbi Q9HCS2.
    PRIDEi Q9HCS2.

    Protocols and materials databases

    DNASUi 66002.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000324632 ; ENSP00000321821 ; ENSG00000186204 . [Q9HCS2-1 ]
    ENST00000517734 ; ENSP00000430849 ; ENSG00000186204 . [Q9HCS2-2 ]
    ENST00000548435 ; ENSP00000449703 ; ENSG00000186204 . [Q9HCS2-2 ]
    ENST00000550308 ; ENSP00000448998 ; ENSG00000186204 . [Q9HCS2-1 ]
    GeneIDi 66002.
    KEGGi hsa:66002.
    UCSCi uc002nbl.3. human.

    Organism-specific databases

    CTDi 66002.
    GeneCardsi GC19P015783.
    HGNCi HGNC:18857. CYP4F12.
    MIMi 611485. gene.
    neXtProti NX_Q9HCS2.
    PharmGKBi PA38717.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000233833.
    HOVERGENi HBG000182.
    InParanoidi Q9HCS2.
    KOi K17730.
    OMAi IICVINI.
    OrthoDBi EOG7CNZFK.
    PhylomeDBi Q9HCS2.
    TreeFami TF105088.

    Enzyme and pathway databases

    Reactomei REACT_13645. Eicosanoids.
    REACT_13814. Fatty acids.

    Miscellaneous databases

    GeneWikii CYP4F12.
    GenomeRNAii 66002.
    NextBioi 67510.
    PROi Q9HCS2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9HCS2.
    Bgeei Q9HCS2.
    CleanExi HS_CYP4F12.
    Genevestigatori Q9HCS2.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of CYP4F12, a novel human cytochrome P450."
      Bylund J., Bylund M., Oliw E.H.
      Biochem. Biophys. Res. Commun. 280:892-897(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHARACTERIZATION, VARIANTS MET-16; ASP-76; VAL-90; ARG-188 AND GLY-522.
      Tissue: Liver.
    2. "cDNA cloning and expression of a novel cytochrome p450 (cyp4f12) from human small intestine."
      Hashizume T., Imaoka S., Hiroi T., Terauchi Y., Fujii T., Miyazaki H., Kamataki T., Funae Y.
      Biochem. Biophys. Res. Commun. 280:1135-1141(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHARACTERIZATION, VARIANTS LEU-13; MET-16; ASP-76; VAL-90; ARG-188 AND GLY-522.
      Tissue: Small intestine.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS MET-16; ASP-76; VAL-90; ARG-188 AND GLY-522.
    4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
      , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
      DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS MET-16 AND ASP-76.
      Tissue: Placenta.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS MET-16 AND ASP-76.
      Tissue: Cervix.
    6. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS MET-16; ASP-76; VAL-90; ARG-188 AND GLY-522.

    Entry informationi

    Entry nameiCP4FC_HUMAN
    AccessioniPrimary (citable) accession number: Q9HCS2
    Secondary accession number(s): E7ET51
    , O60389, Q5JPJ7, Q9HCS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2002
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3