Q9HCS2 (CP4FC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 4F12
EC=1.14.14.1

Alternative name(s):
CYPIVF12

Gene names

Name:	CYP4F12
ORF Names:	UNQ568/PRO1129

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. Catalyzes the hydroxylation of the antihistamine ebastine.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Microsome membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed in small intestine, liver, colon and heart. Ref.1 Ref.2
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence EAW84492.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	drug metabolic process Inferred from sequence or structural similarity. Source: UniProtKB epoxygenase P450 pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of blood coagulation Inferred from sequence or structural similarity. Source: UniProtKB pressure natriuresis Inferred from sequence or structural similarity. Source: UniProtKB renal water homeostasis Inferred from sequence or structural similarity. Source: UniProtKB sodium ion homeostasis Inferred from sequence or structural similarity. Source: UniProtKB very long-chain fatty acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB vitamin E metabolic process Inferred from sequence or structural similarity. Source: UniProtKB vitamin K biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular_component	apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alkane 1-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB arachidonic acid epoxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro leukotriene-B4 20-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB vitamin-K-epoxide reductase (warfarin-sensitive) activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Cytochrome P450 4F12		PRO_0000051857
Regions
	Transmembrane	19 – 39	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential
Sites
	Metal binding	468	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	13	1	P → L. Ref.2 Corresponds to variant rs16995376 [ dbSNP | Ensembl ].		VAR_013244
	Natural variant	16	1	T → M. Ref.1 Ref.2 Ref.3 Corresponds to variant rs16995378 [ dbSNP | Ensembl ].		VAR_048459
	Natural variant	76	1	N → D. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs609636 [ dbSNP | Ensembl ].		VAR_013245
	Natural variant	90	1	I → V. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs609290 [ dbSNP | Ensembl ].		VAR_013246
	Natural variant	188	1	C → R. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs2285888 [ dbSNP | Ensembl ].		VAR_013247
	Natural variant	522	1	S → G. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs593818 [ dbSNP | Ensembl ].		VAR_048460

Sequences

	Sequence		Length	Mass (Da)
	Q9HCS2 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: F29C29BA8DB880FE	FASTA	524	60,270
	10 20 30 40 50 60 MSLLSLPWLG LRPVATSPWL LLLLVVGSWL LARILAWTYA FYNNCRRLQC FPQPPKRNWF 70 80 90 100 110 120 WGHLGLITPT EEGLKNSTQM SATYSQGFTI WLGPIIPFIV LCHPDTIRSI TNASAAIAPK 130 140 150 160 170 180 DNLFIRFLKP WLGEGILLSG GDKWSRHRRM LTPAFHFNIL KSYITIFNKS ANIMLDKWQH 190 200 210 220 230 240 LASEGSSCLD MFEHISLMTL DSLQKCIFSF DSHCQERPSE YIATILELSA LVEKRSQHIL 250 260 270 280 290 300 QHMDFLYYLS HDGRRFHRAC RLVHDFTDAV IRERRRTLPT QGIDDFFKDK AKSKTLDFID 310 320 330 340 350 360 VLLLSKDEDG KALSDEDIRA EADTFMFGGH DTTASGLSWV LYNLARHPEY QERCRQEVQE 370 380 390 400 410 420 LLKDRDPKEI EWDDLAQLPF LTMCVKESLR LHPPAPFISR CCTQDIVLPD GRVIPKGITC 430 440 450 460 470 480 LIDIIGVHHN PTVWPDPEVY DPFRFDPENS KGRSPLAFIP FSAGPRNCIG QAFAMAEMKV 490 500 510 520 VLALMLLHFR FLPDHTEPRR KLELIMRAEG GLWLRVEPLN VSLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and expression of CYP4F12, a novel human cytochrome P450." Bylund J., Bylund M., Oliw E.H. Biochem. Biophys. Res. Commun. 280:892-897(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, CHARACTERIZATION, VARIANTS MET-16; ASP-76; VAL-90; ARG-188 AND GLY-522. Tissue: Liver.
[2]	"cDNA cloning and expression of a novel cytochrome p450 (cyp4f12) from human small intestine." Hashizume T., Imaoka S., Hiroi T., Terauchi Y., Fujii T., Miyazaki H., Kamataki T., Funae Y. Biochem. Biophys. Res. Commun. 280:1135-1141(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, CHARACTERIZATION, VARIANTS LEU-13; MET-16; ASP-76; VAL-90; ARG-188 AND GLY-522. Tissue: Small intestine.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS MET-16; ASP-76; VAL-90; ARG-188 AND GLY-522.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS ASP-76; VAL-90; ARG-188 AND GLY-522.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY008841 mRNA. Translation: AAG33247.1. AB035130 mRNA. Translation: BAB18269.1. AB035131 mRNA. Translation: BAB18270.1. AY358977 mRNA. Translation: AAQ89336.1. AC004523 Genomic DNA. Translation: AAC11543.1. AC122702 Genomic DNA. No translation available. CH471106 Genomic DNA. Translation: EAW84492.1. Different initiation.
IPI	IPI00289171.
PIR	JC7594. JC7598.
RefSeq	NP_076433.3. NM_023944.3.
UniGene	Hs.131459.
3D structure databases
ProteinModelPortal	Q9HCS2.
SMR	Q9HCS2. Positions 51-524.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HCS2. 2 interactions.
STRING	9606.ENSP00000321821.
PTM databases
PhosphoSite	Q9HCS2.
Proteomic databases
PaxDb	Q9HCS2.
PRIDE	Q9HCS2.
Protocols and materials databases
DNASU	66002.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000324632; ENSP00000321821; ENSG00000186204. ENST00000550308; ENSP00000448998; ENSG00000186204.
GeneID	66002.
KEGG	hsa:66002.
UCSC	uc002nbl.3. human.
Organism-specific databases
CTD	66002.
GeneCards	GC19P015783.
HGNC	HGNC:18857. CYP4F12.
MIM	611485. gene.
neXtProt	NX_Q9HCS2.
PharmGKB	PA38717.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000233833.
HOVERGEN	HBG000182.
InParanoid	Q9HCS2.
OMA	ICVINIT.
OrthoDB	EOG49CQ7D.
PhylomeDB	Q9HCS2.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9HCS2.
Bgee	Q9HCS2.
CleanEx	HS_CYP4F12.
Genevestigator	Q9HCS2.
GermOnline	ENSG00000186204. Homo sapiens.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	66002.
NextBio	67510.
SOURCE	Search...

Entry information

Entry name

CP4FC_HUMAN

Accession

Primary (citable) accession number: Q9HCS2
Secondary accession number(s): O60389, Q9HCS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.