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Protein

Cytochrome P450 4F12

Gene

CYP4F12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. Catalyzes the hydroxylation of the antihistamine ebastine.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi468Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-211935. Fatty acids.
R-HSA-211979. Eicosanoids.

Chemistry databases

SwissLipidsiSLP:000001652.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4F12 (EC:1.14.14.1)
Alternative name(s):
CYPIVF12
Gene namesi
Name:CYP4F12
ORF Names:UNQ568/PRO1129
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:18857. CYP4F12.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000186204.
PharmGKBiPA38717.

Chemistry databases

ChEMBLiCHEMBL3509589.
DrugBankiDB08868. Fingolimod.

Polymorphism and mutation databases

BioMutaiCYP4F12.
DMDMi313104094.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000518571 – 524Cytochrome P450 4F12Add BLAST524

Proteomic databases

MaxQBiQ9HCS2.
PaxDbiQ9HCS2.
PeptideAtlasiQ9HCS2.
PRIDEiQ9HCS2.

PTM databases

iPTMnetiQ9HCS2.
PhosphoSitePlusiQ9HCS2.

Expressioni

Tissue specificityi

Expressed in small intestine, liver, colon and heart.2 Publications

Gene expression databases

BgeeiENSG00000186204.
CleanExiHS_CYP4F12.
ExpressionAtlasiQ9HCS2. baseline and differential.
GenevisibleiQ9HCS2. HS.

Organism-specific databases

HPAiHPA058960.

Interactioni

Protein-protein interaction databases

BioGridi122449. 7 interactors.
IntActiQ9HCS2. 4 interactors.
STRINGi9606.ENSP00000321821.

Structurei

3D structure databases

ProteinModelPortaliQ9HCS2.
SMRiQ9HCS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiQ9HCS2.
KOiK17730.
PhylomeDBiQ9HCS2.
TreeFamiTF105088.

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HCS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLSLPWLG LRPVATSPWL LLLLVVGSWL LARILAWTYA FYNNCRRLQC
60 70 80 90 100
FPQPPKRNWF WGHLGLITPT EEGLKNSTQM SATYSQGFTI WLGPIIPFIV
110 120 130 140 150
LCHPDTIRSI TNASAAIAPK DNLFIRFLKP WLGEGILLSG GDKWSRHRRM
160 170 180 190 200
LTPAFHFNIL KSYITIFNKS ANIMLDKWQH LASEGSSCLD MFEHISLMTL
210 220 230 240 250
DSLQKCIFSF DSHCQERPSE YIATILELSA LVEKRSQHIL QHMDFLYYLS
260 270 280 290 300
HDGRRFHRAC RLVHDFTDAV IRERRRTLPT QGIDDFFKDK AKSKTLDFID
310 320 330 340 350
VLLLSKDEDG KALSDEDIRA EADTFMFGGH DTTASGLSWV LYNLARHPEY
360 370 380 390 400
QERCRQEVQE LLKDRDPKEI EWDDLAQLPF LTMCVKESLR LHPPAPFISR
410 420 430 440 450
CCTQDIVLPD GRVIPKGITC LIDIIGVHHN PTVWPDPEVY DPFRFDPENS
460 470 480 490 500
KGRSPLAFIP FSAGPRNCIG QAFAMAEMKV VLALMLLHFR FLPDHTEPRR
510 520
KLELIMRAEG GLWLRVEPLN VSLQ
Length:524
Mass (Da):60,270
Last modified:November 30, 2010 - v2
Checksum:iF29C29BA8DB880FE
GO
Isoform 2 (identifier: Q9HCS2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-102: IWLGPIIPFIVLC → LPLHPRIISSSGS
     103-524: Missing.

Note: No experimental confirmation available.
Show »
Length:102
Mass (Da):11,572
Checksum:i356BE81361CDE5A9
GO

Sequence cautioni

The sequence EAW84492 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01324413P → L1 PublicationCorresponds to variant dbSNP:rs16995376Ensembl.1
Natural variantiVAR_04845916T → M6 PublicationsCorresponds to variant dbSNP:rs16995378Ensembl.1
Natural variantiVAR_01324576N → D6 PublicationsCorresponds to variant dbSNP:rs609636Ensembl.1
Natural variantiVAR_01324690I → V4 PublicationsCorresponds to variant dbSNP:rs609290Ensembl.1
Natural variantiVAR_013247188C → R4 PublicationsCorresponds to variant dbSNP:rs2285888Ensembl.1
Natural variantiVAR_048460522S → G4 PublicationsCorresponds to variant dbSNP:rs593818Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05558190 – 102IWLGP…FIVLC → LPLHPRIISSSGS in isoform 2. 2 PublicationsAdd BLAST13
Alternative sequenceiVSP_055582103 – 524Missing in isoform 2. 2 PublicationsAdd BLAST422

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008841 mRNA. Translation: AAG33247.1.
AB035130 mRNA. Translation: BAB18269.1.
AB035131 mRNA. Translation: BAB18270.1.
AY358977 mRNA. Translation: AAQ89336.1.
AK075435 mRNA. Translation: BAG52137.1.
AL832171 mRNA. Translation: CAI46131.1.
AC004523 Genomic DNA. Translation: AAC11543.1.
AC122702 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84492.1. Different initiation.
CH471106 Genomic DNA. Translation: EAW84495.1.
CCDSiCCDS42517.1. [Q9HCS2-1]
PIRiJC7594.
JC7598.
RefSeqiNP_076433.3. NM_023944.3.
UniGeneiHs.131459.

Genome annotation databases

EnsembliENST00000517734; ENSP00000430849; ENSG00000186204. [Q9HCS2-2]
ENST00000548435; ENSP00000449703; ENSG00000186204. [Q9HCS2-2]
GeneIDi66002.
KEGGihsa:66002.
UCSCiuc060uvi.1. human. [Q9HCS2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCP4FC_HUMAN
AccessioniPrimary (citable) accession number: Q9HCS2
Secondary accession number(s): E7ET51
, O60389, Q5JPJ7, Q9HCS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: November 30, 2010
Last modified: August 30, 2017
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families