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Protein

Pro-FMRFamide-related neuropeptide VF

Gene

NPVF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia.2 Publications

GO - Biological processi

  • negative regulation of gonadotropin secretion Source: UniProtKB
  • neuropeptide signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-FMRFamide-related neuropeptide VF
Cleaved into the following 4 chains:
Gene namesi
Name:NPVF
Synonyms:C7orf9, RFRP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:13782. NPVF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162398161.

Polymorphism and mutation databases

BioMutaiNPVF.
DMDMi311033508.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Propeptidei27 – 5529PRO_0000009920Add
BLAST
Peptidei56 – 9237Neuropeptide NPSFSequence analysisPRO_0000009921Add
BLAST
Peptidei81 – 9212Neuropeptide RFRP-1PRO_0000401171Add
BLAST
Propeptidei95 – 995PRO_0000009922
Peptidei101 – 11212Neuropeptide RFRP-2Sequence analysisPRO_0000009923Add
BLAST
Propeptidei115 – 1217PRO_0000009924
Peptidei124 – 1318Neuropeptide NPVFPRO_0000009925
Propeptidei134 – 19663PRO_0000009926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei92 – 921Phenylalanine amide1 Publication
Modified residuei131 – 1311Phenylalanine amide1 Publication

Keywords - PTMi

Amidation, Cleavage on pair of basic residues

Proteomic databases

PaxDbiQ9HCQ7.
PeptideAtlasiQ9HCQ7.
PRIDEiQ9HCQ7.

PTM databases

iPTMnetiQ9HCQ7.
PhosphoSiteiQ9HCQ7.

Expressioni

Tissue specificityi

Isoform 1 is specifically expressed in the retina. Neuropeptides RFRP-1 and NPVF are detected in the hypothalamus.2 Publications

Gene expression databases

BgeeiQ9HCQ7.
CleanExiHS_NPVF.
GenevisibleiQ9HCQ7. HS.

Organism-specific databases

HPAiHPA041733.

Interactioni

Protein-protein interaction databases

IntActiQ9HCQ7. 3 interactions.
MINTiMINT-7241363.
STRINGi9606.ENSP00000222674.

Structurei

3D structure databases

ProteinModelPortaliQ9HCQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWCW. Eukaryota.
ENOG410Z21X. LUCA.
HOGENOMiHOG000133054.
HOVERGENiHBG031581.
InParanoidiQ9HCQ7.
OrthoDBiEOG70S76V.
PhylomeDBiQ9HCQ7.
TreeFamiTF330935.

Family and domain databases

InterProiIPR026297. FMRFamide-related/fGRP.
[Graphical view]
PANTHERiPTHR14403. PTHR14403. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HCQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEIISSKLFI LLTLATSSLL TSNIFCADEL VISNLHSKEN YDKYSEPRGY
60 70 80 90 100
PKGERSLNFE ELKDWGPKNV IKMSTPAVNK MPHSFANLPL RFGRNVQEER
110 120 130 140 150
SAGATANLPL RSGRNMEVSL VRRVPNLPQR FGRTTTAKSV CRMLSDLCQG
160 170 180 190
SMHSPCANDL FYSMTCQHQE IQNPDQKQSR RLLFKKIDDA ELKQEK
Length:196
Mass (Da):22,309
Last modified:November 2, 2010 - v2
Checksum:i67764DFC30CD59A0
GO
Isoform 2 (identifier: Q9HCQ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     181-196: Missing.

Show »
Length:180
Mass (Da):20,352
Checksum:i764FA673A3A3A38F
GO

Mass spectrometryi

Molecular mass is 1428.85 Da from positions 81 - 92. Determined by MALDI. 1 Publication
Molecular mass is 969.56 Da from positions 124 - 131. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti32 – 321I → M.3 Publications
Corresponds to variant rs886354 [ dbSNP | Ensembl ].
VAR_014073
Natural varianti42 – 421D → G.1 Publication
Corresponds to variant rs877834 [ dbSNP | Ensembl ].
VAR_014074
Natural varianti121 – 1211V → M.
Corresponds to variant rs3213641 [ dbSNP | Ensembl ].
VAR_030644

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei181 – 19616Missing in isoform 2. 1 PublicationVSP_039962Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040290 mRNA. Translation: BAB17674.1.
AF330057 mRNA. Translation: AAK94201.1.
AF440392 mRNA. Translation: AAL90453.1.
AF440395, AF440393, AF440394 Genomic DNA. Translation: AAL90458.1.
AC004129 Genomic DNA. Translation: AAP22339.1.
CH236948 Genomic DNA. Translation: EAL24237.1.
CH471073 Genomic DNA. Translation: EAW93830.1.
CCDSiCCDS5395.1. [Q9HCQ7-1]
RefSeqiNP_071433.3. NM_022150.3.
UniGeneiHs.60473.

Genome annotation databases

EnsembliENST00000222674; ENSP00000222674; ENSG00000105954.
GeneIDi64111.
KEGGihsa:64111.
UCSCiuc003sxo.4. human. [Q9HCQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040290 mRNA. Translation: BAB17674.1.
AF330057 mRNA. Translation: AAK94201.1.
AF440392 mRNA. Translation: AAL90453.1.
AF440395, AF440393, AF440394 Genomic DNA. Translation: AAL90458.1.
AC004129 Genomic DNA. Translation: AAP22339.1.
CH236948 Genomic DNA. Translation: EAL24237.1.
CH471073 Genomic DNA. Translation: EAW93830.1.
CCDSiCCDS5395.1. [Q9HCQ7-1]
RefSeqiNP_071433.3. NM_022150.3.
UniGeneiHs.60473.

3D structure databases

ProteinModelPortaliQ9HCQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9HCQ7. 3 interactions.
MINTiMINT-7241363.
STRINGi9606.ENSP00000222674.

PTM databases

iPTMnetiQ9HCQ7.
PhosphoSiteiQ9HCQ7.

Polymorphism and mutation databases

BioMutaiNPVF.
DMDMi311033508.

Proteomic databases

PaxDbiQ9HCQ7.
PeptideAtlasiQ9HCQ7.
PRIDEiQ9HCQ7.

Protocols and materials databases

DNASUi64111.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222674; ENSP00000222674; ENSG00000105954.
GeneIDi64111.
KEGGihsa:64111.
UCSCiuc003sxo.4. human. [Q9HCQ7-1]

Organism-specific databases

CTDi64111.
GeneCardsiNPVF.
HGNCiHGNC:13782. NPVF.
HPAiHPA041733.
neXtProtiNX_Q9HCQ7.
PharmGKBiPA162398161.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IWCW. Eukaryota.
ENOG410Z21X. LUCA.
HOGENOMiHOG000133054.
HOVERGENiHBG031581.
InParanoidiQ9HCQ7.
OrthoDBiEOG70S76V.
PhylomeDBiQ9HCQ7.
TreeFamiTF330935.

Miscellaneous databases

GenomeRNAii64111.
PROiQ9HCQ7.

Gene expression databases

BgeeiQ9HCQ7.
CleanExiHS_NPVF.
GenevisibleiQ9HCQ7. HS.

Family and domain databases

InterProiIPR026297. FMRFamide-related/fGRP.
[Graphical view]
PANTHERiPTHR14403. PTHR14403. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SYNTHESIS OF RFRP-1; RFRP-2 AND NPVF, FUNCTION.
    Tissue: Fetal brain.
  2. "Identification and characterization of novel mammalian neuropeptide FF-like peptides that attenuate morphine-induced antinociception."
    Liu Q., Guan X.-M., Martin W.J., McDonald T.P., Clements M.K., Jiang Q., Zeng Z., Jacobson M., Williams D.L. Jr., Yu H., Bomford D., Figueroa D., Mallee J., Wang R., Evans J., Gould R., Austin C.P.
    J. Biol. Chem. 276:36961-36969(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT MET-32.
  3. "Genomic structure and assessment of the retinally expressed RFamide-related peptide gene in dominant cystoid macular dystrophy."
    Schulz H.L., Stoehr H., White K., van Driel M.A., Hoyng C.B., Cremers F., Weber B.H.F.
    Mol. Vis. 8:67-71(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT GLY-42.
    Tissue: Retina.
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT MET-32.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT MET-32.
  7. "Identification of human GnIH homologs, RFRP-1 and RFRP-3, and the cognate receptor, GPR147 in the human hypothalamic pituitary axis."
    Ubuka T., Morgan K., Pawson A.J., Osugi T., Chowdhury V.S., Minakata H., Tsutsui K., Millar R.P., Bentley G.E.
    PLoS ONE 4:E8400-E8400(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 81-92 AND 124-131, AMIDATION AT PHE-92 AND PHE-131, FUNCTION, TISSUE SPECIFICITY, MASS SPECTROMETRY.

Entry informationi

Entry nameiNPVF_HUMAN
AccessioniPrimary (citable) accession number: Q9HCQ7
Secondary accession number(s): A4D164, Q7LE27, Q96PI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: November 2, 2010
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.