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Q9HCQ5

- GALT9_HUMAN

UniProt

Q9HCQ5 - GALT9_HUMAN

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Protein

Polypeptide N-acetylgalactosaminyltransferase 9

Gene

GALNT9

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Does not glycosylate apomucin or SDC3.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.1 Publication

Cofactori

Manganese.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei191 – 1911SubstrateBy similarity
Binding sitei222 – 2221SubstrateBy similarity
Metal bindingi245 – 2451ManganeseBy similarity
Metal bindingi247 – 2471ManganeseBy similarity
Metal bindingi377 – 3771ManganeseBy similarity
Binding sitei380 – 3801SubstrateBy similarity
Binding sitei385 – 3851SubstrateBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. O-glycan processing Source: Reactome
  3. post-translational protein modification Source: Reactome
  4. protein O-linked glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.41. 2681.
ReactomeiREACT_115606. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 9 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 9
Short name:
GalNAc-T9
Short name:
pp-GaNTase 9
Protein-UDP acetylgalactosaminyltransferase 9
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9
Gene namesi
Name:GALNT9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:4131. GALNT9.

Subcellular locationi

GO - Cellular componenti

  1. Golgi membrane Source: Reactome
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28544.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 603603Polypeptide N-acetylgalactosaminyltransferase 9PRO_0000059120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi141 ↔ 372PROSITE-ProRule annotation
Disulfide bondi363 ↔ 442PROSITE-ProRule annotation
Glycosylationi460 – 4601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi477 ↔ 493PROSITE-ProRule annotation
Disulfide bondi525 ↔ 540PROSITE-ProRule annotation
Disulfide bondi567 ↔ 587PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9HCQ5.
PRIDEiQ9HCQ5.

Expressioni

Tissue specificityi

Specifically expressed in brain. Not expressed in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte. In brain, it is expressed in cerebellum, frontal lobe, temporal lobe, putamen and spinal cord, weakly expressed in cerebral cortex. Not expressed in medulla and occipital pole.1 Publication

Gene expression databases

BgeeiQ9HCQ5.
CleanExiHS_GALNT9.
ExpressionAtlasiQ9HCQ5. baseline and differential.
GenevestigatoriQ9HCQ5.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000380488.

Structurei

3D structure databases

ProteinModelPortaliQ9HCQ5.
SMRiQ9HCQ5. Positions 99-592.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Topological domaini30 – 603574LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2923Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini464 – 600137Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 261112Catalytic subdomain AAdd
BLAST
Regioni318 – 38063Catalytic subdomain BAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250827.
GeneTreeiENSGT00670000097647.
HOGENOMiHOG000038228.
HOVERGENiHBG051699.
InParanoidiQ9HCQ5.
KOiK00710.
PhylomeDBiQ9HCQ5.
TreeFamiTF313267.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9HCQ5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVARKIRTL LTVNILVFVG IVLFSVYCRL QGRSQELVRI VSGDRRVRSR
60 70 80 90 100
HAKVGTLGDR EAILQRLDHL EEVVYNQLNG LAKPIGLVEG PGGLGQGGLA
110 120 130 140 150
ATLRDDGQEA EGKYEEYGYN AQLSDRISLD RSIPDYRPRK CRQMSYAQDL
160 170 180 190 200
PQVSVVFIFV NEALSVILRS VHSVVNHTPS QLLKEVILVD DNSDNVELKF
210 220 230 240 250
NLDQYVNKRY PGLVKIVRNS RREGLIRARL QGWKAATAPV VGFFDAHVEF
260 270 280 290 300
NTGWAEPALS RIREDRRRIV LPAIDNIKYS TFEVQQYANA AHGYNWGLRC
310 320 330 340 350
MYIIPPQDWL DRGDESAPIR TPAMIGCSFV VDREYFGDIG LLDPGMEVYG
360 370 380 390 400
GENVELGMRV WQCGGSMEVL PCSRVAHIER TRKPYNNDID YYAKRNALRA
410 420 430 440 450
AEVWMDDFKS HVYMAWNIPM SNPGVDFGDV SERLALRQRL KCRSFKWYLE
460 470 480 490 500
NVYPEMRVYN NTLTYGEVRN SKASAYCLDQ GAEDGDRAIL YPCHGMSSQL
510 520 530 540 550
VRYSADGLLQ LGPLGSTAFL PDSKCLVDDG TGRMPTLKKC EDVARPTQRL
560 570 580 590 600
WDFTQSGPIV SRATGRCLEV EMSKDANFGL RLVVQRCSGQ KWMIRNWIKH

ARH
Length:603
Mass (Da):68,359
Last modified:April 3, 2007 - v3
Checksum:i4B5888733D8577CF
GO
Isoform 2 (identifier: Q9HCQ5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-366: Missing.

Show »
Length:237
Mass (Da):27,211
Checksum:i5484F64E65F6F6DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti539 – 5391K → R in BAB13699. (PubMed:10978536)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 366366Missing in isoform 2. 2 PublicationsVSP_011206Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040672 mRNA. Translation: BAB13699.2.
AF458594 mRNA. Translation: AAM49722.1.
BC093817 mRNA. Translation: AAH93817.2.
BC093819 mRNA. Translation: AAH93819.2.
CCDSiCCDS41866.1. [Q9HCQ5-2]
RefSeqiNP_001116108.1. NM_001122636.1.
NP_068580.2. NM_021808.3. [Q9HCQ5-2]
UniGeneiHs.301062.
Hs.658249.

Genome annotation databases

EnsembliENST00000397325; ENSP00000380488; ENSG00000182870. [Q9HCQ5-2]
ENST00000541995; ENSP00000440544; ENSG00000182870. [Q9HCQ5-2]
GeneIDi50614.
KEGGihsa:50614.
UCSCiuc001uka.3. human. [Q9HCQ5-2]

Polymorphism databases

DMDMi143811393.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Polypeptide N-acetylgalactosaminyltransferase 9

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040672 mRNA. Translation: BAB13699.2 .
AF458594 mRNA. Translation: AAM49722.1 .
BC093817 mRNA. Translation: AAH93817.2 .
BC093819 mRNA. Translation: AAH93819.2 .
CCDSi CCDS41866.1. [Q9HCQ5-2 ]
RefSeqi NP_001116108.1. NM_001122636.1.
NP_068580.2. NM_021808.3. [Q9HCQ5-2 ]
UniGenei Hs.301062.
Hs.658249.

3D structure databases

ProteinModelPortali Q9HCQ5.
SMRi Q9HCQ5. Positions 99-592.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000380488.

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Polymorphism databases

DMDMi 143811393.

Proteomic databases

PaxDbi Q9HCQ5.
PRIDEi Q9HCQ5.

Protocols and materials databases

DNASUi 50614.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000397325 ; ENSP00000380488 ; ENSG00000182870 . [Q9HCQ5-2 ]
ENST00000541995 ; ENSP00000440544 ; ENSG00000182870 . [Q9HCQ5-2 ]
GeneIDi 50614.
KEGGi hsa:50614.
UCSCi uc001uka.3. human. [Q9HCQ5-2 ]

Organism-specific databases

CTDi 50614.
GeneCardsi GC12M132680.
HGNCi HGNC:4131. GALNT9.
MIMi 606251. gene.
neXtProti NX_Q9HCQ5.
PharmGKBi PA28544.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG250827.
GeneTreei ENSGT00670000097647.
HOGENOMi HOG000038228.
HOVERGENi HBG051699.
InParanoidi Q9HCQ5.
KOi K00710.
PhylomeDBi Q9HCQ5.
TreeFami TF313267.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.41. 2681.
Reactomei REACT_115606. O-linked glycosylation of mucins.

Miscellaneous databases

GenomeRNAii 50614.
NextBioi 53114.
PROi Q9HCQ5.
SOURCEi Search...

Gene expression databases

Bgeei Q9HCQ5.
CleanExi HS_GALNT9.
ExpressionAtlasi Q9HCQ5. baseline and differential.
Genevestigatori Q9HCQ5.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
IPR001202. WW_dom.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Brain-specific expression of a novel human UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T9)."
    Toba S., Tenno M., Konishi M., Mikami T., Itoh N., Kurosaka A.
    Biochim. Biophys. Acta 1493:264-268(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Brain.
  2. Guo J.H., Zan Q., Yu L.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Colon.
  4. "Cloning and characterization of a new human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, designated pp-GalNAc-T13, that is specifically expressed in neurons and synthesizes GalNAc alpha-serine/threonine antigen."
    Zhang Y., Iwasaki H., Wang H., Kudo T., Kalka T.B., Hennet T., Kubota T., Cheng L., Inaba N., Gotoh M., Togayachi A., Guo J.-M., Hisatomi H., Nakajima K., Nishihara S., Nakamura M., Marth J.D., Narimatsu H.
    J. Biol. Chem. 278:573-584(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME ACTIVITY.

Entry informationi

Entry nameiGALT9_HUMAN
AccessioniPrimary (citable) accession number: Q9HCQ5
Secondary accession number(s): Q52LR8, Q6NT54, Q8NFR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: April 3, 2007
Last modified: October 29, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3