Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Casein kinase I isoform gamma-1

Gene

CSNK1G1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Repressed by 2-(2-hydroxyethylamino)-6-(3-chloroanilino)-9-isopropylpurine (DB08325).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731ATPPROSITE-ProRule annotation
Active sitei164 – 1641Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi50 – 589ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiQ9HCP0.
SIGNORiQ9HCP0.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform gamma-1 (EC:2.7.11.1)
Short name:
CKI-gamma 1
Gene namesi
Name:CSNK1G1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:2454. CSNK1G1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi169 – 1691N → A: Loss of kinase activity. 1 Publication

Organism-specific databases

PharmGKBiPA26954.

Chemistry

ChEMBLiCHEMBL2111458.
GuidetoPHARMACOLOGYi1999.

Polymorphism and mutation databases

BioMutaiCSNK1G1.
DMDMi18202957.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422Casein kinase I isoform gamma-1PRO_0000192839Add
BLAST

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9HCP0.
MaxQBiQ9HCP0.
PaxDbiQ9HCP0.
PRIDEiQ9HCP0.

PTM databases

iPTMnetiQ9HCP0.
PhosphoSiteiQ9HCP0.
SwissPalmiQ9HCP0.

Expressioni

Gene expression databases

BgeeiQ9HCP0.
CleanExiHS_CSNK1G1.
ExpressionAtlasiQ9HCP0. baseline and differential.
GenevisibleiQ9HCP0. HS.

Organism-specific databases

HPAiHPA018209.

Interactioni

Subunit structurei

Monomer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP76Q8TAP63EBI-1383814,EBI-742887
KRTAP10-7P604093EBI-1383814,EBI-10172290

Protein-protein interaction databases

BioGridi119824. 16 interactions.
IntActiQ9HCP0. 9 interactions.
MINTiMINT-4650731.
STRINGi9606.ENSP00000305777.

Chemistry

BindingDBiQ9HCP0.

Structurei

Secondary structure

1
422
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi49 – 524Combined sources
Beta strandi54 – 629Combined sources
Turni64 – 663Combined sources
Beta strandi69 – 768Combined sources
Helixi84 – 9411Combined sources
Beta strandi104 – 1107Combined sources
Beta strandi113 – 1197Combined sources
Helixi125 – 1317Combined sources
Turni132 – 1343Combined sources
Helixi138 – 15720Combined sources
Helixi167 – 1693Combined sources
Beta strandi170 – 1723Combined sources
Turni175 – 1784Combined sources
Beta strandi183 – 1853Combined sources
Turni197 – 1993Combined sources
Turni215 – 2173Combined sources
Helixi220 – 2234Combined sources
Helixi230 – 24617Combined sources
Turni250 – 2534Combined sources
Helixi259 – 27214Combined sources
Helixi275 – 2784Combined sources
Turni279 – 2813Combined sources
Helixi284 – 29512Combined sources
Helixi304 – 31714Combined sources
Turni327 – 3304Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CMWX-ray1.75A45-352[»]
ProteinModelPortaliQ9HCP0.
SMRiQ9HCP0. Positions 45-339.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HCP0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 315272Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1165. Eukaryota.
ENOG410XNXP. LUCA.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182054.
HOVERGENiHBG000176.
InParanoidiQ9HCP0.
KOiK08958.
OrthoDBiEOG7G1V66.
PhylomeDBiQ9HCP0.
TreeFamiTF313349.

Family and domain databases

InterProiIPR022247. Casein_kinase-1_gamma_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12605. CK1gamma_C. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1L (identifier: Q9HCP0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDHPSREKDE RQRTTKPMAQ RSAHCSRPSG SSSSSGVLMV GPNFRVGKKI
60 70 80 90 100
GCGNFGELRL GKNLYTNEYV AIKLEPIKSR APQLHLEYRF YKQLGSAGEG
110 120 130 140 150
LPQVYYFGPC GKYNAMVLEL LGPSLEDLFD LCDRTFTLKT VLMIAIQLLS
160 170 180 190 200
RMEYVHSKNL IYRDVKPENF LIGRQGNKKE HVIHIIDFGL AKEYIDPETK
210 220 230 240 250
KHIPYREHKS LTGTARYMSI NTHLGKEQSR RDDLEALGHM FMYFLRGSLP
260 270 280 290 300
WQGLKADTLK ERYQKIGDTK RNTPIEALCE NFPEEMATYL RYVRRLDFFE
310 320 330 340 350
KPDYEYLRTL FTDLFEKKGY TFDYAYDWVG RPIPTPVGSV HVDSGASAIT
360 370 380 390 400
RESHTHRDRP SQQQPLRNQV VSSTNGELNV DDPTGAHSNA PITAHAEVEV
410 420
VEEAKCCCFF KRKRKKTAQR HK
Length:422
Mass (Da):48,511
Last modified:March 1, 2001 - v1
Checksum:iB875922349B97645
GO
Isoform 1S (identifier: Q9HCP0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-422: VVSSTNGELN...KRKKTAQRHK → SLRTVTAEHYDVNNSAIWHRGRGT

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:393
Mass (Da):45,414
Checksum:i2979A77D92F62261
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti206 – 2061R → K.1 Publication
Corresponds to variant rs55799101 [ dbSNP | Ensembl ].
VAR_042084
Natural varianti329 – 3291V → I.1 Publication
Corresponds to variant rs55699712 [ dbSNP | Ensembl ].
VAR_042085

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei370 – 42253VVSST…AQRHK → SLRTVTAEHYDVNNSAIWHR GRGT in isoform 1S. 1 PublicationVSP_004748Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042563 mRNA. Translation: BAB17839.1.
AB042562 mRNA. Translation: BAB17838.1.
AL832551 mRNA. Translation: CAI46142.1.
CH471082 Genomic DNA. Translation: EAW77670.1.
BC017236 mRNA. Translation: AAH17236.2.
BC130613 mRNA. Translation: AAI30614.1.
BC136504 mRNA. Translation: AAI36505.1.
CCDSiCCDS10192.2. [Q9HCP0-1]
RefSeqiNP_071331.2. NM_022048.3.
UniGeneiHs.646508.

Genome annotation databases

EnsembliENST00000303052; ENSP00000305777; ENSG00000169118. [Q9HCP0-1]
ENST00000606225; ENSP00000475325; ENSG00000169118. [Q9HCP0-2]
GeneIDi53944.
KEGGihsa:53944.
UCSCiuc002anf.4. human. [Q9HCP0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042563 mRNA. Translation: BAB17839.1.
AB042562 mRNA. Translation: BAB17838.1.
AL832551 mRNA. Translation: CAI46142.1.
CH471082 Genomic DNA. Translation: EAW77670.1.
BC017236 mRNA. Translation: AAH17236.2.
BC130613 mRNA. Translation: AAI30614.1.
BC136504 mRNA. Translation: AAI36505.1.
CCDSiCCDS10192.2. [Q9HCP0-1]
RefSeqiNP_071331.2. NM_022048.3.
UniGeneiHs.646508.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CMWX-ray1.75A45-352[»]
ProteinModelPortaliQ9HCP0.
SMRiQ9HCP0. Positions 45-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119824. 16 interactions.
IntActiQ9HCP0. 9 interactions.
MINTiMINT-4650731.
STRINGi9606.ENSP00000305777.

Chemistry

BindingDBiQ9HCP0.
ChEMBLiCHEMBL2111458.
GuidetoPHARMACOLOGYi1999.

PTM databases

iPTMnetiQ9HCP0.
PhosphoSiteiQ9HCP0.
SwissPalmiQ9HCP0.

Polymorphism and mutation databases

BioMutaiCSNK1G1.
DMDMi18202957.

Proteomic databases

EPDiQ9HCP0.
MaxQBiQ9HCP0.
PaxDbiQ9HCP0.
PRIDEiQ9HCP0.

Protocols and materials databases

DNASUi53944.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303052; ENSP00000305777; ENSG00000169118. [Q9HCP0-1]
ENST00000606225; ENSP00000475325; ENSG00000169118. [Q9HCP0-2]
GeneIDi53944.
KEGGihsa:53944.
UCSCiuc002anf.4. human. [Q9HCP0-1]

Organism-specific databases

CTDi53944.
GeneCardsiCSNK1G1.
HGNCiHGNC:2454. CSNK1G1.
HPAiHPA018209.
MIMi606274. gene.
neXtProtiNX_Q9HCP0.
PharmGKBiPA26954.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1165. Eukaryota.
ENOG410XNXP. LUCA.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182054.
HOVERGENiHBG000176.
InParanoidiQ9HCP0.
KOiK08958.
OrthoDBiEOG7G1V66.
PhylomeDBiQ9HCP0.
TreeFamiTF313349.

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiQ9HCP0.
SIGNORiQ9HCP0.

Miscellaneous databases

ChiTaRSiCSNK1G1. human.
EvolutionaryTraceiQ9HCP0.
GenomeRNAii53944.
PROiQ9HCP0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9HCP0.
CleanExiHS_CSNK1G1.
ExpressionAtlasiQ9HCP0. baseline and differential.
GenevisibleiQ9HCP0. HS.

Family and domain databases

InterProiIPR022247. Casein_kinase-1_gamma_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12605. CK1gamma_C. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression analysis and chromosome mapping of human casein kinase 1 gamma1 (CSNK1G1): identification of two types of cDNA encoding the kinase protein associated with heterologous carboxy-terminal sequences."
    Kusuda J., Hirai M., Tanuma R., Hashimoto K.
    Cytogenet. Cell Genet. 90:298-302(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1L AND 1S).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1L).
    Tissue: Muscle.
  5. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
  6. "Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1."
    Meng Z., Capalbo L., Glover D.M., Dunphy W.G.
    Mol. Biol. Cell 22:2834-2847(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS CLSPN KINASE, MUTAGENESIS OF ASN-169.
  7. "Structure of human casein kinase 1 gamma-1 in complex with (3-chloroanilino)-9-isopropylpurine (casp target)."
    Bunkoczi G., Rellos P., Das S., Savitsky P., Niesen F., Sobott F., Fedorov O., Pike A.C.W., Von Delft F., Sundstrom M., Arrowsmith C., Edwards A., Weigelt J., Knapp S.
    Submitted (JUL-2011) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 45-352 IN COMPLEX WITH DB08325.
  8. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] LYS-206 AND ILE-329.

Entry informationi

Entry nameiKC1G1_HUMAN
AccessioniPrimary (citable) accession number: Q9HCP0
Secondary accession number(s): Q5JPH1, Q96AE9, Q9HCP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.