Reviewed,
UniProtKB/Swiss-Prot Q9HCL2 (GPAT1_HUMAN)
Last modified
February 9, 2010.
Version 76.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glycerol-3-phosphate acyltransferase 1, mitochondrial Short name=GPAT-1 EC=2.3.1.15 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 828 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Ref.4 |
| Catalytic activity | Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. |
| Pathway | |
| Subcellular location | Mitochondrion outer membrane; Multi-pass membrane protein By similarity. |
| Domain | The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. |
| Sequence similarities | Belongs to the GPAT/DAPAT family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Phospholipid biosynthesis |
| Cellular component | Membrane Mitochondrion Mitochondrion outer membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Transit peptide Transmembrane |
| Molecular function | Acyltransferase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW triglyceride biosynthetic process Ref.4Inferred from direct assay. Source: UniProtKB |
| Cellular component | cytosol Inferred from Experiment. Source: Reactome integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | glycerol-3-phosphate O-acyltransferase activity Ref.4 Inferred from mutant phenotype. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion Potential | |||||||
| Chain | ? – 828 | Glycerol-3-phosphate acyltransferase 1, mitochondrial | PRO_0000024690 | ||||||
Regions | |||||||||
| Topological domain | ? – 471 | Mitochondrial intermembrane Potential | |||||||
| Transmembrane | 472 – 494 | 23 | Potential | ||||||
| Topological domain | 495 – 574 | 80 | Cytoplasmic Potential | ||||||
| Transmembrane | 575 – 593 | 19 | Potential | ||||||
| Topological domain | 594 – 828 | 235 | Mitochondrial intermembrane Potential | ||||||
| Motif | 230 – 235 | 6 | HXXXXD motif | ||||||
Amino acid modifications | |||||||||
| Modified residue | 111 | 1 | Phosphotyrosine Ref.3 | ||||||
| Modified residue | 695 | 1 | Phosphoserine Ref.5 | ||||||
Natural variations | |||||||||
| Natural variant | 4 | 1 | S → Y: dbSNP rs11549703. | VAR_050585 | |||||
| Natural variant | 43 | 1 | I → V: dbSNP rs2792751. | VAR_050586 | |||||
| Natural variant | 131 | 1 | E → G: dbSNP rs10787428. | VAR_050587 | |||||
| Natural variant | 386 | 1 | I → T: dbSNP rs35019520. | VAR_050588 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. Rogers J.Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [2] | "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O. DNA Res. 7:273-281(2000) [PubMed: 10997877] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-828. Tissue: Brain. |
| [3] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-111, MASS SPECTROMETRY. Tissue: Epithelium. |
| [4] | "AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase." Chen Y.Q., Kuo M.-S., Li S., Bui H.H., Peake D.A., Sanders P.E., Thibodeaux S.J., Chu S., Qian Y.-W., Zhao Y., Bredt D.S., Moller D.E., Konrad R.J., Beigneux A.P., Young S.G., Cao G. J. Biol. Chem. 283:10048-10057(2008) [PubMed: 18238778] [Abstract] Cited for: FUNCTION. |
| [5] | "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-695, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AL391986 Genomic DNA. Translation: CAH73716.1. AB046780 mRNA. Translation: BAB13386.1. |
| IPI | IPI00844249. |
| RefSeq | NP_065969.3. |
| UniGene | Hs.42586 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9HCL2. |
PTM databases | |
| PhosphoSite | Q9HCL2. |
Proteomic databases | |
| PRIDE | Q9HCL2. |
Genome annotation databases | |
| Ensembl | ENST00000348367; ENSP00000265276; ENSG00000119927; Homo sapiens. [Genome view] ENST00000423155; ENSP00000409242; ENSG00000119927; Homo sapiens. [Genome view] |
| GeneID | 57678. |
| KEGG | hsa:57678. |
| NMPDR | fig|9606.3.peg.4647. |
| UCSC | uc001kzp.2. human. |
Organism-specific databases | |
| CTD | 57678. |
| GeneCards | GC10M113899. |
| H-InvDB | HIX0009205. |
| HGNC | HGNC:24865. GPAM. |
| MIM | 602395. gene. |
| PharmGKB | PA134983031. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG12513. |
| HOGENOM | HBG443870. |
| HOVERGEN | Q9HCL2. |
| InParanoid | Q9HCL2. |
| OMA | DCYLKVS. |
| OrthoDB | EOG9JWZ0T. |
| PhylomeDB | Q9HCL2. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:57678-MONOMER. |
| BRENDA | 2.3.1.15. 247. |
| Reactome | REACT_602. Metabolism of lipids and lipoproteins. |
Gene expression databases | |
| ArrayExpress | Q9HCL2. |
| Bgee | Q9HCL2. |
| CleanEx | HS_GPAM. |
| Genevestigator | Q9HCL2. |
| GermOnline | ENSG00000119927. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR002123. Acyltransferase. [Graphical view] |
| Pfam | PF01553. Acyltransferase. 1 hit. [Graphical view] |
| SMART | SM00563. PlsC. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 64486. |
| SOURCE | Search... |
Entry information
| Entry name | GPAT1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9HCL2 Secondary accession number(s): Q5VW51 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 10 Human chromosome 10: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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