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Q9HCL2

- GPAT1_HUMAN

UniProt

Q9HCL2 - GPAT1_HUMAN

Protein

Glycerol-3-phosphate acyltransferase 1, mitochondrial

Gene

GPAM

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.1 Publication

    Catalytic activityi

    Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. glycerol-3-phosphate O-acyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. acyl-CoA metabolic process Source: Ensembl
    2. CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
    3. cellular lipid metabolic process Source: Reactome
    4. defense response to virus Source: Ensembl
    5. fatty acid homeostasis Source: Ensembl
    6. fatty acid metabolic process Source: Ensembl
    7. glycerophospholipid biosynthetic process Source: Reactome
    8. interleukin-2 secretion Source: Ensembl
    9. negative regulation of activation-induced cell death of T cells Source: Ensembl
    10. phosphatidic acid biosynthetic process Source: Reactome
    11. phospholipid homeostasis Source: Ensembl
    12. phospholipid metabolic process Source: Reactome
    13. positive regulation of activated T cell proliferation Source: Ensembl
    14. positive regulation of multicellular organism growth Source: Ensembl
    15. regulation of cytokine secretion Source: Ensembl
    16. response to glucose Source: Ensembl
    17. small molecule metabolic process Source: Reactome
    18. triglyceride biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:57678-MONOMER.
    ReactomeiREACT_1190. Triglyceride Biosynthesis.
    REACT_120906. Synthesis of PA.
    REACT_147904. Activation of gene expression by SREBF (SREBP).
    UniPathwayiUPA00557; UER00612.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate acyltransferase 1, mitochondrial (EC:2.3.1.15)
    Short name:
    GPAT-1
    Gene namesi
    Name:GPAM
    Synonyms:GPAT1, KIAA1560
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:24865. GPAM.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: Ensembl
    3. mitochondrial outer membrane Source: Reactome
    4. plasma membrane Source: InterPro

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134983031.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 828Glycerol-3-phosphate acyltransferase 1, mitochondrialPRO_0000024690
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei380 – 3801PhosphoserineBy similarity
    Modified residuei688 – 6881PhosphoserineBy similarity
    Modified residuei695 – 6951Phosphoserine1 Publication
    Modified residuei780 – 7801N6-acetyllysineBy similarity
    Modified residuei784 – 7841N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9HCL2.
    PaxDbiQ9HCL2.
    PRIDEiQ9HCL2.

    PTM databases

    PhosphoSiteiQ9HCL2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9HCL2.
    BgeeiQ9HCL2.
    CleanExiHS_GPAM.
    GenevestigatoriQ9HCL2.

    Organism-specific databases

    HPAiHPA046339.

    Interactioni

    Protein-protein interaction databases

    BioGridi121707. 2 interactions.
    IntActiQ9HCL2. 1 interaction.
    STRINGi9606.ENSP00000265276.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HCL2.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini? – 471Mitochondrial intermembraneSequence Analysis
    Topological domaini495 – 57480CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini594 – 828235Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei472 – 49423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei575 – 59319HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi230 – 2356HXXXXD motif

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

    Sequence similaritiesi

    Belongs to the GPAT/DAPAT family.Curated

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2937.
    HOGENOMiHOG000112780.
    HOVERGENiHBG000102.
    InParanoidiQ9HCL2.
    KOiK00629.
    OMAiKKNESLW.
    OrthoDBiEOG74R1PZ.
    PhylomeDBiQ9HCL2.
    TreeFamiTF313360.

    Family and domain databases

    InterProiIPR022284. GPAT/DHAPAT.
    IPR028354. GPAT_PlsB.
    IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view]
    PANTHERiPTHR12563. PTHR12563. 1 hit.
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500064. GPAT. 1 hit.
    PIRSF000437. GPAT_DHAPAT. 1 hit.
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9HCL2-1 [UniParc]FASTAAdd to Basket

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    MDESALTLGT IDVSYLPHSS EYSVGRCKHT SEEWGECGFR PTIFRSATLK    50
    WKESLMSRKR PFVGRCCYSC TPQSWDKFFN PSIPSLGLRN VIYINETHTR 100
    HRGWLARRLS YVLFIQERDV HKGMFATNVT ENVLNSSRVQ EAIAEVAAEL 150
    NPDGSAQQQS KAVNKVKKKA KRILQEMVAT VSPAMIRLTG WVLLKLFNSF 200
    FWNIQIHKGQ LEMVKAATET NLPLLFLPVH RSHIDYLLLT FILFCHNIKA 250
    PYIASGNNLN IPIFSTLIHK LGGFFIRRRL DETPDGRKDV LYRALLHGHI 300
    VELLRQQQFL EIFLEGTRSR SGKTSCARAG LLSVVVDTLS TNVIPDILII 350
    PVGISYDRII EGHYNGEQLG KPKKNESLWS VARGVIRMLR KNYGCVRVDF 400
    AQPFSLKEYL ESQSQKPVSA LLSLEQALLP AILPSRPSDA ADEGRDTSIN 450
    ESRNATDESL RRRLIANLAE HILFTASKSC AIMSTHIVAC LLLYRHRQGI 500
    DLSTLVEDFF VMKEEVLARD FDLGFSGNSE DVVMHAIQLL GNCVTITHTS 550
    RNDEFFITPS TTVPSVFELN FYSNGVLHVF IMEAIIACSL YAVLNKRGLG 600
    GPTSTPPNLI SQEQLVRKAA SLCYLLSNEG TISLPCQTFY QVCHETVGKF 650
    IQYGILTVAE HDDQEDISPS LAEQQWDKKL PEPLSWRSDE EDEDSDFGEE 700
    QRDCYLKVSQ SKEHQQFITF LQRLLGPLLE AYSSAAIFVH NFSGPVPEPE 750
    YLQKLHKYLI TRTERNVAVY AESATYCLVK NAVKMFKDIG VFKETKQKRV 800
    SVLELSSTFL PQCNRQKLLE YILSFVVL 828
    Length:828
    Mass (Da):93,795
    Last modified:February 15, 2005 - v3
    Checksum:i7AADD21BC8684693
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti204 – 2041I → F in CAD89932. (PubMed:17974005)Curated
    Sequence conflicti652 – 6521Q → R in CAD89932. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti4 – 41S → Y.
    Corresponds to variant rs11549703 [ dbSNP | Ensembl ].
    VAR_050585
    Natural varianti43 – 431I → V.
    Corresponds to variant rs2792751 [ dbSNP | Ensembl ].
    VAR_050586
    Natural varianti131 – 1311E → G.
    Corresponds to variant rs10787428 [ dbSNP | Ensembl ].
    VAR_050587
    Natural varianti386 – 3861I → T.
    Corresponds to variant rs35019520 [ dbSNP | Ensembl ].
    VAR_050588

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL833093 mRNA. Translation: CAD89932.1.
    AL391986 Genomic DNA. Translation: CAH73716.1.
    AB046780 mRNA. Translation: BAB13386.1.
    CCDSiCCDS7570.1.
    RefSeqiNP_001231878.1. NM_001244949.1.
    NP_065969.3. NM_020918.5.
    XP_005270055.1. XM_005269998.1.
    UniGeneiHs.42586.

    Genome annotation databases

    EnsembliENST00000348367; ENSP00000265276; ENSG00000119927.
    GeneIDi57678.
    KEGGihsa:57678.
    UCSCiuc001kzp.3. human.

    Polymorphism databases

    DMDMi59803040.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL833093 mRNA. Translation: CAD89932.1 .
    AL391986 Genomic DNA. Translation: CAH73716.1 .
    AB046780 mRNA. Translation: BAB13386.1 .
    CCDSi CCDS7570.1.
    RefSeqi NP_001231878.1. NM_001244949.1.
    NP_065969.3. NM_020918.5.
    XP_005270055.1. XM_005269998.1.
    UniGenei Hs.42586.

    3D structure databases

    ProteinModelPortali Q9HCL2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121707. 2 interactions.
    IntActi Q9HCL2. 1 interaction.
    STRINGi 9606.ENSP00000265276.

    PTM databases

    PhosphoSitei Q9HCL2.

    Polymorphism databases

    DMDMi 59803040.

    Proteomic databases

    MaxQBi Q9HCL2.
    PaxDbi Q9HCL2.
    PRIDEi Q9HCL2.

    Protocols and materials databases

    DNASUi 57678.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000348367 ; ENSP00000265276 ; ENSG00000119927 .
    GeneIDi 57678.
    KEGGi hsa:57678.
    UCSCi uc001kzp.3. human.

    Organism-specific databases

    CTDi 57678.
    GeneCardsi GC10M113899.
    HGNCi HGNC:24865. GPAM.
    HPAi HPA046339.
    MIMi 602395. gene.
    neXtProti NX_Q9HCL2.
    PharmGKBi PA134983031.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2937.
    HOGENOMi HOG000112780.
    HOVERGENi HBG000102.
    InParanoidi Q9HCL2.
    KOi K00629.
    OMAi KKNESLW.
    OrthoDBi EOG74R1PZ.
    PhylomeDBi Q9HCL2.
    TreeFami TF313360.

    Enzyme and pathway databases

    UniPathwayi UPA00557 ; UER00612 .
    BioCyci MetaCyc:57678-MONOMER.
    Reactomei REACT_1190. Triglyceride Biosynthesis.
    REACT_120906. Synthesis of PA.
    REACT_147904. Activation of gene expression by SREBF (SREBP).

    Miscellaneous databases

    ChiTaRSi GPAM. human.
    GeneWikii GPAM.
    GenomeRNAii 57678.
    NextBioi 64486.
    PROi Q9HCL2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9HCL2.
    Bgeei Q9HCL2.
    CleanExi HS_GPAM.
    Genevestigatori Q9HCL2.

    Family and domain databases

    InterProi IPR022284. GPAT/DHAPAT.
    IPR028354. GPAT_PlsB.
    IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view ]
    PANTHERi PTHR12563. PTHR12563. 1 hit.
    Pfami PF01553. Acyltransferase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500064. GPAT. 1 hit.
    PIRSF000437. GPAT_DHAPAT. 1 hit.
    SMARTi SM00563. PlsC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Spinal cord.
    2. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.
      DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 167-828.
      Tissue: Brain.
    4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. Cited for: FUNCTION.
    6. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
      Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
      Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-695, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGPAT1_HUMAN
    AccessioniPrimary (citable) accession number: Q9HCL2
    Secondary accession number(s): Q5VW51, Q86TA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3