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Protein

Nck-associated protein 5-like

Gene

NCKAP5L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates microtubule organization and stabilization. Promotes microtubule growth and bundling formation and stabilizes microtubules by increasing intense acetylation of microtubules (PubMed:26482847, PubMed:26485573). Both tubulin-binding and homodimer formation are required for NCKAP5L-mediated microtubule bundle formation (PubMed:26485573).2 Publications

GO - Biological processi

  • microtubule bundle formation Source: UniProtKB
  • microtubule depolymerization Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nck-associated protein 5-like
Short name:
NCKAP5-like
Alternative name(s):
Centrosomal protein of 169 kDa1 Publication
Short name:
Cep1691 Publication
Gene namesi
Name:NCKAP5L
Synonyms:CEP1691 Publication, KIAA1602
ORF Names:FP1193
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:29321. NCKAP5L.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW
  • microtubule plus-end Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi482 – 483IP → AA: No decrease in localization to microtubule plus ends. Loss of interaction with MAPRE1 and localization to microtubule plus ends; when associated with 814-A-A-815 and 924-A-A-925. 2 Publications2
Mutagenesisi814 – 815LP → AA: No decrease in localization to microtubule plus ends. Loss of interaction with MAPRE1 and localization to microtubule plus ends; when associated with 482-A-A-483 and 924-A-A-925. 2 Publications2
Mutagenesisi924 – 925LP → AA: Loss of interaction with MAPRE1 and significantly reduced localization to microtubule plus ends. Loss of interaction with MAPRE1 and localization to microtubule plus ends; when associated with 482-A-A-483 and 814-A-A-815. 2 Publications2

Organism-specific databases

PharmGKBiPA165513124.

Polymorphism and mutation databases

BioMutaiNCKAP5L.
DMDMi156630840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002884471 – 1330Nck-associated protein 5-likeAdd BLAST1330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei436Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei447Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei466Phosphoserine; by CDK11 Publication1
Modified residuei473Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei489PhosphoserineCombined sources1
Modified residuei492PhosphoserineCombined sources1
Modified residuei494PhosphoserineCombined sources1
Modified residuei567Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei573Phosphoserine; by CDK11 Publication1
Modified residuei655PhosphothreonineCombined sources1
Modified residuei763Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei1190PhosphoserineCombined sources1

Post-translational modificationi

CDK1/Cyclin B-dependent phosphorylation mediates its dissociation from centrosomes during mitosis.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9HCH0.
MaxQBiQ9HCH0.
PaxDbiQ9HCH0.
PeptideAtlasiQ9HCH0.
PRIDEiQ9HCH0.

PTM databases

iPTMnetiQ9HCH0.
PhosphoSitePlusiQ9HCH0.

Expressioni

Gene expression databases

BgeeiENSG00000167566.
CleanExiHS_KIAA1602.
ExpressionAtlasiQ9HCH0. baseline and differential.
GenevisibleiQ9HCH0. HS.

Organism-specific databases

HPAiHPA041034.

Interactioni

Subunit structurei

Homodimer (PubMed:26482847). Interacts with CDK5RAP2 (PubMed:26485573). Interacts with MAPRE1 (PubMed:26485573). Interacts with beta-tubulin (PubMed:26482847).2 Publications

Protein-protein interaction databases

BioGridi121726. 24 interactors.
IntActiQ9HCH0. 23 interactors.
STRINGi9606.ENSP00000337998.

Structurei

3D structure databases

ProteinModelPortaliQ9HCH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 135Mediates interaction with CDK5RAP2 and is required for homodimerization and microtubule bundle formation2 PublicationsAdd BLAST135
Regioni746 – 1142Mediates interaction with beta-tubulin and is required for microtubule bundle formation1 PublicationAdd BLAST397

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili24 – 102Sequence analysisAdd BLAST79
Coiled coili952 – 990Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi480 – 483(S/T)X(I/L)P motif 12 Publications4
Motifi812 – 815(S/T)X(I/L)P motif 22 Publications4
Motifi922 – 925(S/T)X(I/L)P motif 3; required for interaction with MAPRE12 Publications4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi112 – 614Pro-richAdd BLAST503
Compositional biasi784 – 875Pro-richAdd BLAST92
Compositional biasi1042 – 1283Pro-richAdd BLAST242

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IHBY. Eukaryota.
ENOG410XSUM. LUCA.
HOGENOMiHOG000113170.
HOVERGENiHBG080689.
InParanoidiQ9HCH0.
OrthoDBiEOG091G007P.
PhylomeDBiQ9HCH0.
TreeFamiTF331208.

Family and domain databases

InterProiIPR032769. NCKAP5_C.
IPR026163. Nckap5l.
[Graphical view]
PANTHERiPTHR21740. PTHR21740. 1 hit.
PfamiPF15246. NCKAP5. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HCH0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDQPAGGPGN PRPGEGDDGS MEPGTCQELL HRLRELEAEN SALAQANENQ
60 70 80 90 100
RETYERCLDE VANHVVQALL NQKDLREECI KLKKRVFDLE RQNQMLSALF
110 120 130 140 150
QQKLQLTTGS LPQIPLTPLQ PPSEPPASPS LSSTEGPAAP LPLGHCAGQR
160 170 180 190 200
EVCWEQQLRP GGPGPPAAPP PALDALSPFL RKKAQILEVL RALEETDPLL
210 220 230 240 250
LCSPATPWRP PGQGPGSPEP INGELCGPPQ PEPSPWAPCL LLGPGNLGGL
260 270 280 290 300
LHWERLLGGL GGEEDTGRPW GPSRGPPQAQ GTSSGPNCAP GSSSSSSSDE
310 320 330 340 350
AGDPNEAPSP DTLLGALARR QLNLGQLLED TESYLQAFLA GAAGPLNGDH
360 370 380 390 400
PGPGQSSSPD QAPPQLSKSK GLPKSAWGGG TPEAHRPGFG ATSEGQGPLP
410 420 430 440 450
FLSMFMGAGD APLGSRPGHP HSSSQVKSKL QIGPPSPGEA QGPLLPSPAR
460 470 480 490 500
GLKFLKLPPT SEKSPSPGGP QLSPQLPRNS RIPCRNSGSD GSPSPLLARR
510 520 530 540 550
GLGGGELSPE GAQGLPTSPS PCYTTPDSTQ LRPPQSALST TLSPGPVVSP
560 570 580 590 600
CYENILDLSR STFRGPSPEP PPSPLQVPTY PQLTLEVPQA PEVLRSPGVP
610 620 630 640 650
PSPCLPESYP YGSPQEKSLD KAGSESPHPG RRTPGNSSKK PSQGSGRRPG
660 670 680 690 700
DPGSTPLRDR LAALGKLKTG PEGALGSEKN GVPARPGTEK TRGPGKSGES
710 720 730 740 750
AGDMVPSIHR PLEQLEAKGG IRGAVALGTN SLKQQEPGLM GDPGARVYSS
760 770 780 790 800
HSMGARVDLE PVSPRSCLTK VELAKSRLAG ALCPQVPRTP AKVPTSAPSL
810 820 830 840 850
GKPNKSPHSS PTKLPSKSPT KVVPRPGAPL VTKESPKPDK GKGPPWADCG
860 870 880 890 900
STTAQSTPLV PGPTDPSQGP EGLAPHSAIE EKVMKGIEEN VLRLQGQERA
910 920 930 940 950
PGAEVKHRNT SSIASWFGLK KSKLPALNRR TEATKNKEGA GGGSPLRREV
960 970 980 990 1000
KMEARKLEAE SLNISKLMAK AEDLRRALEE EKAYLSSRAR PRPGGPAPGP
1010 1020 1030 1040 1050
NTGLGQVQGQ LAGMYQGADT FMQQLLNRVD GKELPSKSWR EPKPEYGDFQ
1060 1070 1080 1090 1100
PVSSDPKSPW PACGPRNGLV GPLQGCGKPP GKPSSEPGRR EETPSEDSLA
1110 1120 1130 1140 1150
EPVPTSHFTA CGSLTRTLDS GIGTFPPPDH GSSGTPSKNL PKTKPPRLDP
1160 1170 1180 1190 1200
PPGVPPARPP PLTKVPRRAH TLEREVPGIE ELLVSGRHPS MPAFPALLPA
1210 1220 1230 1240 1250
APGHRGHETC PDDPCEDPGP TPPVQLAKNW TFPNTRAAGS SSDPLMCPPR
1260 1270 1280 1290 1300
QLEGLPRTPM ALPVDRKRSQ EPSRPSPTPQ GPPFGGSRTP STSDMAEEGR
1310 1320 1330
VASGGPPGLE TSESLSDSLY DSLSSCGSQG
Length:1,330
Mass (Da):139,013
Last modified:May 29, 2007 - v2
Checksum:iF1BF45DF48445342
GO
Isoform 2 (identifier: Q9HCH0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1261-1330: ALPVDRKRSQ...DSLSSCGSQG → VRIAAEERERTREQEGVMWGDQFLQ

Note: No experimental confirmation available.
Show »
Length:1,285
Mass (Da):134,913
Checksum:i9BD104E362412201
GO
Isoform 4 (identifier: Q9HCH0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-108: ANHVVQALLN...LFQQKLQLTT → CGSVVGLGGC...GTGVAEPEGE
     109-1330: Missing.

Note: No experimental confirmation available.
Show »
Length:108
Mass (Da):11,186
Checksum:i4C26FEC3CB3F5D90
GO
Isoform 3 (identifier: Q9HCH0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     771-792: Missing.

Note: No experimental confirmation available.
Show »
Length:1,308
Mass (Da):136,726
Checksum:i14CCB310858BB187
GO

Sequence cautioni

The sequence AAQ15202 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1093T → M in AAH07998 (PubMed:15489334).Curated1
Sequence conflicti1093T → M in AAH33253 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03381862 – 108ANHVV…LQLTT → CGSVVGLGGCGSSAPGRSWG QLMALPRGFLSPGCQPCGTG VAEPEGE in isoform 4. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_033819109 – 1330Missing in isoform 4. 1 PublicationAdd BLAST1222
Alternative sequenceiVSP_025677771 – 792Missing in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_0256781261 – 1330ALPVD…CGSQG → VRIAAEERERTREQEGVMWG DQFLQ in isoform 2. 1 PublicationAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131157 Genomic DNA. No translation available.
BC007998 mRNA. Translation: AAH07998.1.
BC033253 mRNA. Translation: AAH33253.1.
BC110599 mRNA. Translation: AAI10600.1.
AB046822 mRNA. Translation: BAB13428.1.
AF370366 mRNA. Translation: AAQ15202.1. Frameshift.
RefSeqiNP_001032895.2. NM_001037806.3.
UniGeneiHs.143067.

Genome annotation databases

EnsembliENST00000335999; ENSP00000337998; ENSG00000167566.
GeneIDi57701.
KEGGihsa:57701.
UCSCiuc009zlk.2. human. [Q9HCH0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC131157 Genomic DNA. No translation available.
BC007998 mRNA. Translation: AAH07998.1.
BC033253 mRNA. Translation: AAH33253.1.
BC110599 mRNA. Translation: AAI10600.1.
AB046822 mRNA. Translation: BAB13428.1.
AF370366 mRNA. Translation: AAQ15202.1. Frameshift.
RefSeqiNP_001032895.2. NM_001037806.3.
UniGeneiHs.143067.

3D structure databases

ProteinModelPortaliQ9HCH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121726. 24 interactors.
IntActiQ9HCH0. 23 interactors.
STRINGi9606.ENSP00000337998.

PTM databases

iPTMnetiQ9HCH0.
PhosphoSitePlusiQ9HCH0.

Polymorphism and mutation databases

BioMutaiNCKAP5L.
DMDMi156630840.

Proteomic databases

EPDiQ9HCH0.
MaxQBiQ9HCH0.
PaxDbiQ9HCH0.
PeptideAtlasiQ9HCH0.
PRIDEiQ9HCH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335999; ENSP00000337998; ENSG00000167566.
GeneIDi57701.
KEGGihsa:57701.
UCSCiuc009zlk.2. human. [Q9HCH0-1]

Organism-specific databases

CTDi57701.
GeneCardsiNCKAP5L.
H-InvDBHIX0010612.
HGNCiHGNC:29321. NCKAP5L.
HPAiHPA041034.
MIMi615104. gene.
neXtProtiNX_Q9HCH0.
PharmGKBiPA165513124.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHBY. Eukaryota.
ENOG410XSUM. LUCA.
HOGENOMiHOG000113170.
HOVERGENiHBG080689.
InParanoidiQ9HCH0.
OrthoDBiEOG091G007P.
PhylomeDBiQ9HCH0.
TreeFamiTF331208.

Miscellaneous databases

ChiTaRSiNCKAP5L. human.
GenomeRNAii57701.
PROiQ9HCH0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167566.
CleanExiHS_KIAA1602.
ExpressionAtlasiQ9HCH0. baseline and differential.
GenevisibleiQ9HCH0. HS.

Family and domain databases

InterProiIPR032769. NCKAP5_C.
IPR026163. Nckap5l.
[Graphical view]
PANTHERiPTHR21740. PTHR21740. 1 hit.
PfamiPF15246. NCKAP5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCK5L_HUMAN
AccessioniPrimary (citable) accession number: Q9HCH0
Secondary accession number(s): Q2TB26
, Q71RH1, Q8N4W1, Q96HX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 30, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.