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Q9HCE9 (ANO8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anoctamin-8
Alternative name(s):
Transmembrane protein 16H
Gene names
Name:ANO8
Synonyms:KIAA1623, TMEM16H
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1232 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Does not exhibit calcium-activated chloride channel (CaCC) activity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Shows predominantly an intracellular localization with a weak expression in the cell membrane. Ref.6 Ref.12

Tissue specificity

Expressed in embryonic stem cells, fetal brain and neural tissues. Ref.5

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Sequence similarities

Belongs to the anoctamin family.

Sequence caution

The sequence BAB13449.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9HCE9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9HCE9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1112-1114: TAL → HKL
     1115-1232: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13
Chain2 – 12321231Anoctamin-8
PRO_0000249003

Regions

Topological domain2 – 244243Extracellular Potential
Transmembrane245 – 26521Helical; Potential
Topological domain266 – 28116Cytoplasmic Potential
Transmembrane282 – 30221Helical; Potential
Topological domain303 – 35654Extracellular Potential
Transmembrane357 – 37721Helical; Potential
Topological domain378 – 40023Cytoplasmic Potential
Transmembrane401 – 42121Helical; Potential
Topological domain422 – 43716Extracellular Potential
Transmembrane438 – 45821Helical; Potential
Topological domain459 – 750292Cytoplasmic Potential
Transmembrane751 – 77121Helical; Potential
Topological domain772 – 80736Extracellular Potential
Transmembrane808 – 82821Helical; Potential
Topological domain829 – 84113Cytoplasmic Potential
Transmembrane842 – 86221Helical; Potential
Topological domain863 – 1232370Extracellular Potential
Compositional bias353 – 518166Leu-rich
Compositional bias533 – 58048Gly-rich
Compositional bias554 – 673120Glu-rich
Compositional bias1130 – 122293Pro-rich

Amino acid modifications

Modified residue21N-acetylalanine Ref.13

Natural variations

Alternative sequence1112 – 11143TAL → HKL in isoform 2.
VSP_020351
Alternative sequence1115 – 1232118Missing in isoform 2.
VSP_020352

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 3.
Checksum: 1802BC3544A9234C

FASTA1,232136,034
        10         20         30         40         50         60 
MAEAASGAGG TSLEGERGKR PPPEGEPAAP ASGVLDKLFG KRLLQAGRYL VSHKAWMKTV 

        70         80         90        100        110        120 
PTENCDVLMT FPDTTDDHTL LWLLNHIRVG IPELIVQVRH HRHTRAYAFF VTATYESLLR 

       130        140        150        160        170        180 
GADELGLRKA VKAEFGGGTR GFSCEEDFIY ENVESELRFF TSQERQSIIR FWLQNLRAKQ 

       190        200        210        220        230        240 
GEALHNVRFL EDQPIIPELA ARGIIQQVFP VHEQRILNRL MKSWVQAVCE NQPLDDICDY 

       250        260        270        280        290        300 
FGVKIAMYFA WLGFYTSAMV YPAVFGSVLY TFTEADQTSR DVSCVVFALF NVIWSTLFLE 

       310        320        330        340        350        360 
EWKRRGAELA YKWGTLDSPG EAVEEPRPQF RGVRRISPIT RAEEFYYPPW KRLLFQLLVS 

       370        380        390        400        410        420 
LPLCLACLVC VFLLMLGCFQ LQELVLSVKG LPRLARFLPK VMLALLVSVS AEGYKKLAIW 

       430        440        450        460        470        480 
LNDMENYRLE SAYEKHLIIK VVLFQFVNSY LSLFYIGFYL KDMERLKEML ATLLITRQFL 

       490        500        510        520        530        540 
QNVREVLQPH LYRRLGRGEL GLRAVWELAR ALLGLLSLRR PAPRRLEPQA DEGGGGGSGG 

       550        560        570        580        590        600 
GGRRCLSGGC GAPEEEEEAA LVERRRAGEG GEEGDGPPGG KEEDEDDEEE EDEEEEEDEE 

       610        620        630        640        650        660 
EGEEGGLLDC GLRLKKVSFA ERGAGRRRPG PSPEALLEEG SPTMVEKGLE PGVFTLAEED 

       670        680        690        700        710        720 
DEAEGAPGSP EREPPAILFR RAGGEGRDQG PDGGPDPEPG SNSDSTRRQR RQNRSSWIDP 

       730        740        750        760        770        780 
PEEEHSPQLT QAELESCMKK YEDTFQDYQE MFVQFGYVVL FSSAFPLAAL CALVNNLIEI 

       790        800        810        820        830        840 
RSDAFKLCTG LQRPFGQRVE SIGQWQKVME AMGVLAIVVN CYLIGQCGQL QRLFPWLSPE 

       850        860        870        880        890        900 
AAIVSVVVLE HFALLLKYLI HVAIPDIPGW VAEEMAKLEY QRREAFKRHE RQAQHRYQQQ 

       910        920        930        940        950        960 
QRRRREEEER QRHAEHHARR EHDSGGREEA RAEGSGLDPA TSSEKASAKA KGSTAGGHGP 

       970        980        990       1000       1010       1020 
ERPKRPGSLL APNNVMKLKQ IIPLQGKFLS SGATSSLAAA GAGATTRPPP AQSPTGSDTR 

      1030       1040       1050       1060       1070       1080 
LPAFLSFKFL KSPETRRDSE RSHSPPKAFH AGKLFPFGGT RAEPGSNGAG GQARPDGTPS 

      1090       1100       1110       1120       1130       1140 
SGSSRVQRSG PVDEALAEEL EAPRPEEEGS GTALAPVGAP ALRTRRSRSP APPPPMPLPR 

      1150       1160       1170       1180       1190       1200 
PPTPPAGCWQ WDGPWGCGGE GAAPRQALAA AECPPCAMAG PPPAPQPLPG DASFYSLPPP 

      1210       1220       1230 
PLPPTSDPLE TPAPSPSPSP SPQAVCWPSG WH 

« Hide

Isoform 2 [UniParc].

Checksum: 7BA90E3E817E4110
Show »

FASTA1,114124,151

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.
DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Identification and characterization of TMEM16H gene in silico."
Katoh M., Katoh M.
Int. J. Mol. Med. 15:353-358(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
[6]"Expression and function of epithelial anoctamins."
Schreiber R., Uliyakina I., Kongsuphol P., Warth R., Mirza M., Martins J.R., Kunzelmann K.
J. Biol. Chem. 285:7838-7845(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY, SUBCELLULAR LOCATION.
[7]"Physiological roles and diseases of Tmem16/Anoctamin proteins: are they all chloride channels?"
Duran C., Hartzell H.C.
Acta Pharmacol. Sin. 32:685-692(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[8]"CFTR and TMEM16A are separate but functionally related Cl-channels."
Ousingsawat J., Kongsuphol P., Schreiber R., Kunzelmann K.
Cell. Physiol. Biochem. 28:715-724(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY.
[9]"Anoctamins."
Kunzelmann K., Tian Y., Martins J.R., Faria D., Kongsuphol P., Ousingsawat J., Thevenod F., Roussa E., Rock J., Schreiber R.
Pflugers Arch. 462:195-208(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"The anoctamin (TMEM16) gene family: calcium-activated chloride channels come of age."
Winpenny J.P., Gray M.A.
Exp. Physiol. 97:175-176(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[11]"The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels."
Scudieri P., Sondo E., Ferrera L., Galietta L.J.
Exp. Physiol. 97:177-183(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW, ABSENCE OF CALCIUM-ACTIVATED CHLORIDE CHANNEL ACTIVITY.
[12]"Anoctamins are a family of Ca2+ activated Cl- channels."
Tian Y., Schreiber R., Kunzelmann K.
J. Cell Sci. 125:4991-4998(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB046843 mRNA. Translation: BAB13449.2. Different initiation.
AC010463 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84595.1.
CCDSCCDS32949.1. [Q9HCE9-1]
RefSeqNP_066010.1. NM_020959.2. [Q9HCE9-1]
UniGeneHs.590990.

3D structure databases

ProteinModelPortalQ9HCE9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9HCE9. 1 interaction.
STRING9606.ENSP00000159087.

PTM databases

PhosphoSiteQ9HCE9.

Polymorphism databases

DMDM114152287.

Proteomic databases

MaxQBQ9HCE9.
PaxDbQ9HCE9.
PRIDEQ9HCE9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000159087; ENSP00000159087; ENSG00000074855. [Q9HCE9-1]
GeneID57719.
KEGGhsa:57719.
UCSCuc002ngf.2. human. [Q9HCE9-1]

Organism-specific databases

CTD57719.
GeneCardsGC19M017434.
HGNCHGNC:29329. ANO8.
HPAHPA049206.
MIM610216. gene.
neXtProtNX_Q9HCE9.
PharmGKBPA164715790.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG300207.
HOGENOMHOG000033952.
HOVERGENHBG100443.
InParanoidQ9HCE9.
OMAACVFLLM.
PhylomeDBQ9HCE9.
TreeFamTF314265.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ9HCE9.
BgeeQ9HCE9.
CleanExHS_ANO8.
GenevestigatorQ9HCE9.

Family and domain databases

InterProIPR007632. Anoctamin.
[Graphical view]
PANTHERPTHR12308. PTHR12308. 1 hit.
PfamPF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi57719.
NextBio64654.
PROQ9HCE9.
SOURCESearch...

Entry information

Entry nameANO8_HUMAN
AccessionPrimary (citable) accession number: Q9HCE9
Secondary accession number(s): A6NIJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM