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Protein

Anoctamin-8

Gene

ANO8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Does not exhibit calcium-activated chloride channel (CaCC) activity.

GO - Molecular functioni

  • intracellular calcium activated chloride channel activity Source: UniProtKB

GO - Biological processi

  • chloride transport Source: UniProtKB
  • ion transmembrane transport Source: Reactome
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000074855-MONOMER.
ReactomeiR-HSA-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-8
Alternative name(s):
Transmembrane protein 16H
Gene namesi
Name:ANO8
Synonyms:KIAA1623, TMEM16H
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:29329. ANO8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 244ExtracellularSequence analysisAdd BLAST243
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 281CytoplasmicSequence analysisAdd BLAST16
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Topological domaini303 – 356ExtracellularSequence analysisAdd BLAST54
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 400CytoplasmicSequence analysisAdd BLAST23
Transmembranei401 – 421HelicalSequence analysisAdd BLAST21
Topological domaini422 – 437ExtracellularSequence analysisAdd BLAST16
Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
Topological domaini459 – 750CytoplasmicSequence analysisAdd BLAST292
Transmembranei751 – 771HelicalSequence analysisAdd BLAST21
Topological domaini772 – 807ExtracellularSequence analysisAdd BLAST36
Transmembranei808 – 828HelicalSequence analysisAdd BLAST21
Topological domaini829 – 841CytoplasmicSequence analysisAdd BLAST13
Transmembranei842 – 862HelicalSequence analysisAdd BLAST21
Topological domaini863 – 1232ExtracellularSequence analysisAdd BLAST370

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: Ensembl
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57719.
OpenTargetsiENSG00000074855.
PharmGKBiPA164715790.

Polymorphism and mutation databases

BioMutaiANO8.
DMDMi114152287.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002490032 – 1232Anoctamin-8Add BLAST1231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei318PhosphoserineBy similarity1
Modified residuei669PhosphoserineBy similarity1
Modified residuei801Phosphoserine; by FAM20C1 Publication1
Modified residuei1020Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei1020Omega-N-methylarginine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ9HCE9.
PaxDbiQ9HCE9.
PeptideAtlasiQ9HCE9.
PRIDEiQ9HCE9.

PTM databases

iPTMnetiQ9HCE9.
PhosphoSitePlusiQ9HCE9.

Expressioni

Tissue specificityi

Expressed in embryonic stem cells, fetal brain and neural tissues.1 Publication

Gene expression databases

BgeeiENSG00000074855.
CleanExiHS_ANO8.
ExpressionAtlasiQ9HCE9. baseline and differential.
GenevisibleiQ9HCE9. HS.

Organism-specific databases

HPAiHPA031787.
HPA049206.

Interactioni

Protein-protein interaction databases

IntActiQ9HCE9. 1 interactor.
STRINGi9606.ENSP00000159087.

Structurei

3D structure databases

ProteinModelPortaliQ9HCE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 518Leu-richAdd BLAST166
Compositional biasi533 – 580Gly-richAdd BLAST48
Compositional biasi554 – 673Glu-richAdd BLAST120
Compositional biasi1130 – 1222Pro-richAdd BLAST93

Sequence similaritiesi

Belongs to the anoctamin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2513. Eukaryota.
ENOG410XPYE. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000033952.
HOVERGENiHBG100443.
InParanoidiQ9HCE9.
KOiK19502.
OMAiACVFLLM.
OrthoDBiEOG091G06A3.
PhylomeDBiQ9HCE9.
TreeFamiTF314265.

Family and domain databases

InterProiIPR007632. Anoctamin.
IPR031289. Anoctamin-8.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 2 hits.
PTHR12308:SF33. PTHR12308:SF33. 2 hits.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HCE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEAASGAGG TSLEGERGKR PPPEGEPAAP ASGVLDKLFG KRLLQAGRYL
60 70 80 90 100
VSHKAWMKTV PTENCDVLMT FPDTTDDHTL LWLLNHIRVG IPELIVQVRH
110 120 130 140 150
HRHTRAYAFF VTATYESLLR GADELGLRKA VKAEFGGGTR GFSCEEDFIY
160 170 180 190 200
ENVESELRFF TSQERQSIIR FWLQNLRAKQ GEALHNVRFL EDQPIIPELA
210 220 230 240 250
ARGIIQQVFP VHEQRILNRL MKSWVQAVCE NQPLDDICDY FGVKIAMYFA
260 270 280 290 300
WLGFYTSAMV YPAVFGSVLY TFTEADQTSR DVSCVVFALF NVIWSTLFLE
310 320 330 340 350
EWKRRGAELA YKWGTLDSPG EAVEEPRPQF RGVRRISPIT RAEEFYYPPW
360 370 380 390 400
KRLLFQLLVS LPLCLACLVC VFLLMLGCFQ LQELVLSVKG LPRLARFLPK
410 420 430 440 450
VMLALLVSVS AEGYKKLAIW LNDMENYRLE SAYEKHLIIK VVLFQFVNSY
460 470 480 490 500
LSLFYIGFYL KDMERLKEML ATLLITRQFL QNVREVLQPH LYRRLGRGEL
510 520 530 540 550
GLRAVWELAR ALLGLLSLRR PAPRRLEPQA DEGGGGGSGG GGRRCLSGGC
560 570 580 590 600
GAPEEEEEAA LVERRRAGEG GEEGDGPPGG KEEDEDDEEE EDEEEEEDEE
610 620 630 640 650
EGEEGGLLDC GLRLKKVSFA ERGAGRRRPG PSPEALLEEG SPTMVEKGLE
660 670 680 690 700
PGVFTLAEED DEAEGAPGSP EREPPAILFR RAGGEGRDQG PDGGPDPEPG
710 720 730 740 750
SNSDSTRRQR RQNRSSWIDP PEEEHSPQLT QAELESCMKK YEDTFQDYQE
760 770 780 790 800
MFVQFGYVVL FSSAFPLAAL CALVNNLIEI RSDAFKLCTG LQRPFGQRVE
810 820 830 840 850
SIGQWQKVME AMGVLAIVVN CYLIGQCGQL QRLFPWLSPE AAIVSVVVLE
860 870 880 890 900
HFALLLKYLI HVAIPDIPGW VAEEMAKLEY QRREAFKRHE RQAQHRYQQQ
910 920 930 940 950
QRRRREEEER QRHAEHHARR EHDSGGREEA RAEGSGLDPA TSSEKASAKA
960 970 980 990 1000
KGSTAGGHGP ERPKRPGSLL APNNVMKLKQ IIPLQGKFLS SGATSSLAAA
1010 1020 1030 1040 1050
GAGATTRPPP AQSPTGSDTR LPAFLSFKFL KSPETRRDSE RSHSPPKAFH
1060 1070 1080 1090 1100
AGKLFPFGGT RAEPGSNGAG GQARPDGTPS SGSSRVQRSG PVDEALAEEL
1110 1120 1130 1140 1150
EAPRPEEEGS GTALAPVGAP ALRTRRSRSP APPPPMPLPR PPTPPAGCWQ
1160 1170 1180 1190 1200
WDGPWGCGGE GAAPRQALAA AECPPCAMAG PPPAPQPLPG DASFYSLPPP
1210 1220 1230
PLPPTSDPLE TPAPSPSPSP SPQAVCWPSG WH
Length:1,232
Mass (Da):136,034
Last modified:September 5, 2006 - v3
Checksum:i1802BC3544A9234C
GO
Isoform 2 (identifier: Q9HCE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1112-1114: TAL → HKL
     1115-1232: Missing.

Show »
Length:1,114
Mass (Da):124,151
Checksum:i7BA90E3E817E4110
GO

Sequence cautioni

The sequence BAB13449 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0203511112 – 1114TAL → HKL in isoform 2. Curated3
Alternative sequenceiVSP_0203521115 – 1232Missing in isoform 2. CuratedAdd BLAST118

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046843 mRNA. Translation: BAB13449.2. Different initiation.
AC010463 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84595.1.
CCDSiCCDS32949.1. [Q9HCE9-1]
RefSeqiNP_066010.1. NM_020959.2. [Q9HCE9-1]
XP_016882537.1. XM_017027048.1. [Q9HCE9-2]
UniGeneiHs.590990.

Genome annotation databases

EnsembliENST00000159087; ENSP00000159087; ENSG00000074855. [Q9HCE9-1]
GeneIDi57719.
KEGGihsa:57719.
UCSCiuc002ngf.2. human. [Q9HCE9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046843 mRNA. Translation: BAB13449.2. Different initiation.
AC010463 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84595.1.
CCDSiCCDS32949.1. [Q9HCE9-1]
RefSeqiNP_066010.1. NM_020959.2. [Q9HCE9-1]
XP_016882537.1. XM_017027048.1. [Q9HCE9-2]
UniGeneiHs.590990.

3D structure databases

ProteinModelPortaliQ9HCE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9HCE9. 1 interactor.
STRINGi9606.ENSP00000159087.

PTM databases

iPTMnetiQ9HCE9.
PhosphoSitePlusiQ9HCE9.

Polymorphism and mutation databases

BioMutaiANO8.
DMDMi114152287.

Proteomic databases

MaxQBiQ9HCE9.
PaxDbiQ9HCE9.
PeptideAtlasiQ9HCE9.
PRIDEiQ9HCE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000159087; ENSP00000159087; ENSG00000074855. [Q9HCE9-1]
GeneIDi57719.
KEGGihsa:57719.
UCSCiuc002ngf.2. human. [Q9HCE9-1]

Organism-specific databases

CTDi57719.
DisGeNETi57719.
GeneCardsiANO8.
HGNCiHGNC:29329. ANO8.
HPAiHPA031787.
HPA049206.
MIMi610216. gene.
neXtProtiNX_Q9HCE9.
OpenTargetsiENSG00000074855.
PharmGKBiPA164715790.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2513. Eukaryota.
ENOG410XPYE. LUCA.
GeneTreeiENSGT00760000119015.
HOGENOMiHOG000033952.
HOVERGENiHBG100443.
InParanoidiQ9HCE9.
KOiK19502.
OMAiACVFLLM.
OrthoDBiEOG091G06A3.
PhylomeDBiQ9HCE9.
TreeFamiTF314265.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000074855-MONOMER.
ReactomeiR-HSA-2672351. Stimuli-sensing channels.

Miscellaneous databases

ChiTaRSiANO8. human.
GenomeRNAii57719.
PROiQ9HCE9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000074855.
CleanExiHS_ANO8.
ExpressionAtlasiQ9HCE9. baseline and differential.
GenevisibleiQ9HCE9. HS.

Family and domain databases

InterProiIPR007632. Anoctamin.
IPR031289. Anoctamin-8.
[Graphical view]
PANTHERiPTHR12308. PTHR12308. 2 hits.
PTHR12308:SF33. PTHR12308:SF33. 2 hits.
PfamiPF04547. Anoctamin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANO8_HUMAN
AccessioniPrimary (citable) accession number: Q9HCE9
Secondary accession number(s): A6NIJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: November 2, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.