Reviewed,
UniProtKB/Swiss-Prot Q9HCE7 (SMUF1_HUMAN)
Last modified
November 4, 2008.
Version 72.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: E3 ubiquitin-protein ligase SMURF1 EC=6.3.2.- Alternative name(s): Smad ubiquitination regulatory factor 1 Smad-specific E3 ubiquitin ligase 1 Short name=hSMURF1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 757 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. |
| Pathway | |
| Sequence similarities | Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 2 WW domains. |
Ontologies
Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Coding sequence diversity | Alternative splicing |
| Domain | Repeat |
| Molecular function | Ligase |
Gene Ontology (GO) | |
| Biological process | cell differentiation Ref.3 Inferred from direct assay. Source: UniProtKB ectoderm development Ref.3Traceable author statement. Source: UniProtKB negative regulation of BMP signaling pathway Ref.3Traceable author statement. Source: UniProtKB protein ubiquitination during ubiquitin-dependent protein catabolic process Ref.3Inferred from direct assay. Source: UniProtKB |
| Cellular component | intracellular Ref.3 Traceable author statement. Source: UniProtKB |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct ubiquitin-protein ligase activity Ref.3Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: Q9HCE7-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: Q9HCE7-2) The sequence of this isoform differs from the canonical sequence as follows: 269-294: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 757 | 757 | E3 ubiquitin-protein ligase SMURF1 | PRO_0000120326 | |||||
Regions | |||||||||
| Domain | 1 – 99 | 99 | C2 | ||||||
| Domain | 234 – 267 | 34 | WW 1 | ||||||
| Domain | 306 – 339 | 34 | WW 2 | ||||||
| Domain | 420 – 757 | 338 | HECT | ||||||
Sites | |||||||||
| Active site | 725 | 1 | Glycyl thioester intermediate | ||||||
Natural variations | |||||||||
| Alternative sequence | 269 – 294 | 26 | Missing in isoform Short. | VSP_006812 | |||||
Experimental info | |||||||||
| Mutagenesis | 725 | 1 | C → A: Loss of ubiquitination capacity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O. DNA Res. 7:273-281(2000) [PubMed: 10997877] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Brain. |
| [2] | "The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. Wilson R.K.Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation." Zhu H., Kavsak P., Abdollah S., Wrana J.L., Thomsen G.H. Nature 400:687-693(1999) [PubMed: 10458166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-731 (ISOFORM SHORT). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB046845 mRNA. Translation: BAB13451.1. Different initiation. AC004893 Genomic DNA. Translation: AAC62434.1. AC073468 Genomic DNA. No translation available. AC114500 Genomic DNA. No translation available. AF199364 mRNA. Translation: AAF08298.2. | |
| RefSeq | NP_065162.1. NP_851994.1. |
| UniGene | Hs.189329 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1K9R based on UniProtKB P46937. |
| SMR | Q9HCE7. Positions 375-750. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9HCE7. |
Genome annotation databases | |
| Ensembl | ENSG00000198742. Homo sapiens. [Contig view] |
| GeneID | 57154. |
| KEGG | hsa:57154. |
| NMPDR | fig|9606.3.peg.29036. |
Organism-specific databases | |
| H-InvDB | HIX0006884. |
| HGNC | HGNC:16807. SMURF1. |
| MIM | 605568. gene. |
| PharmGKB | PA134987175. |
| HUGE | Search... |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | Q9HCE7. |
| HOVERGEN | Q9HCE7. |
Enzyme and pathway databases | |
| Reactome | REACT_6844. Signaling by TGF beta. |
Gene expression databases | |
| ArrayExpress | Q9HCE7. |
| CleanEx | HS_SMURF1. |
| GermOnline | ENSG00000198742. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000008. C2_Ca-dep. IPR000569. HECT. IPR001202. WW_Rsp5_WWP. [Graphical view] |
| Pfam | PF00168. C2. 1 hit. PF00632. HECT. 1 hit. PF00397. WW. 2 hits. [Graphical view] |
| SMART | SM00239. C2. 1 hit. SM00119. HECTc. 1 hit. SM00456. WW. 2 hits. [Graphical view] |
| PROSITE | PS50004. C2. 1 hit. PS50237. HECT. 1 hit. PS01159. WW_DOMAIN_1. 1 hit. PS50020. WW_DOMAIN_2. 2 hits. [Graphical view] |
| BLOCKS | Search... |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 63133. |
| SOURCE | Search... |
Entry information
| Entry name | SMUF1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9HCE7 Secondary accession number(s): O75853, Q9UJT8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 7 Human chromosome 7: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


