Reviewed, UniProtKB/Swiss-Prot Q9HCC6 (HES4_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcription factor HES-4
Alternative name(s):
    Hairy and enhancer of split 4
      Short name=hHES4
    bHLH factor Hes4

Gene names

Name:

HES4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3' By similarity.
Subunit structure	Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family By similarity.
Subcellular location	Nucleus By similarity.
Domain	Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG) By similarity. The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins By similarity.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 Orange domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein Repressor
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Transcription factor HES-4		PRO_0000127211
Regions
	Domain	48 – 92	45	Helix-loop-helix motif
	Domain	110 – 143	34	Orange
	DNA binding	34 – 47	14	Basic motif
	Motif	216 – 219	4	WRPW motif
	Compositional bias	152 – 218	67	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9HCC6-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: ADFAE485C36EEBC4	FASTA	221	23,523
	10 20 30 40 50 60 MAADTPGKPS ASPMAGAPAS ASRTPDKPRS AAEHRKSSKP VMEKRRRARI NESLAQLKTL 70 80 90 100 110 120 ILDALRKESS RHSKLEKADI LEMTVRHLRS LRRVQVTAAL SADPAVLGKY RAGFHECLAE 130 140 150 160 170 180 VNRFLAGCEG VPADVRSRLL GHLAACLRQL GPSRRPASLS PAAPAEAPAP EVYAGRPLLP 190 200 210 220 SLGGPFPLLA PPLLPGLTRA LPAAPRAGPQ GPGGPWRPWL R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hes7: a bHLH-type repressor gene regulated by Notch and expressed in the presomitic mesoderm." Bessho Y., Miyoshi G., Sakata R., Kageyama R. Genes Cells 6:175-185(2001) [PubMed: 11260262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB048791 mRNA. Translation: BAB13510.1. AL645608 Genomic DNA. Translation: CAI15572.1. BC012351 mRNA. Translation: AAH12351.1.
IPI	IPI00028151.
RefSeq	NP_001135939.1. NP_066993.1.
UniGene	Hs.154029
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9HCC6.
Genome annotation databases
Ensembl	ENSG00000188290. Homo sapiens. [Contig view]
GeneID	57801.
KEGG	hsa:57801.
Organism-specific databases
GeneCards	GC01M000924.
HGNC	HGNC:24149. HES4.
MIM	608060. gene.
PharmGKB	PA134975318.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9HCC6.
HOVERGEN	Q9HCC6.
OMA	Q9HCC6. PWRPWLR.
Gene expression databases
ArrayExpress	Q9HCC6.
Bgee	Q9HCC6.
CleanEx	HS_HES4.
GermOnline	ENSG00000188290. Homo sapiens.
Family and domain databases
InterPro	IPR001092. HLH_basic. IPR011598. HLH_DNA_bd. IPR003650. Orange. IPR018352. Orange_subgroup. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
Pfam	PF07527. Hairy_orange. 1 hit. PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. SM00511. ORANGE. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. PS51054. ORANGE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	64747.
SOURCE	Search...

Entry information

Entry name

HES4_HUMAN

Accession

Primary (citable) accession number: Q9HCC6
Secondary accession number(s): Q5SVA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)