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Protein

Spondin-1

Gene

SPON1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi325 – 3251CalciumCombined sources1 Publication
Metal bindingi354 – 3541CalciumCombined sources1 Publication
Metal bindingi358 – 3581Calcium; via carbonyl oxygenCombined sources1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-5083635. Defective B3GALTL causes Peters-plus syndrome (PpS).
R-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Spondin-1
Alternative name(s):
F-spondin
Vascular smooth muscle cell growth-promoting factor
Gene namesi
Name:SPON1
Synonyms:KIAA0762, VSGP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11252. SPON1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36082.

Polymorphism and mutation databases

BioMutaiSPON1.
DMDMi52783472.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Chaini29 – 807779Spondin-1PRO_0000035865Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 128Combined sources2 Publications
Disulfide bondi156 ↔ 182Combined sources2 Publications
Disulfide bondi199 ↔ 336Combined sources1 Publication
Disulfide bondi200 ↔ 340Combined sources1 Publication
Disulfide bondi202 ↔ 415Combined sources1 Publication
Glycosylationi214 – 2141N-linked (GlcNAc...)Combined sources1 Publication
Disulfide bondi443 ↔ 480PROSITE-ProRule annotation
Glycosylationi448 – 4481C-linked (Man)1 Publication
Glycosylationi451 – 4511C-linked (Man); partial1 Publication
Disulfide bondi454 ↔ 489PROSITE-ProRule annotation
Disulfide bondi459 ↔ 494PROSITE-ProRule annotation
Disulfide bondi502 ↔ 538PROSITE-ProRule annotation
Glycosylationi507 – 5071C-linked (Man)1 Publication
Glycosylationi510 – 5101C-linked (Man); partial1 Publication
Disulfide bondi513 ↔ 517PROSITE-ProRule annotation
Disulfide bondi548 ↔ 554PROSITE-ProRule annotation
Disulfide bondi559 ↔ 595PROSITE-ProRule annotation
Glycosylationi564 – 5641C-linked (Man)1 Publication
Disulfide bondi570 ↔ 574PROSITE-ProRule annotation
Disulfide bondi605 ↔ 610PROSITE-ProRule annotation
Disulfide bondi615 ↔ 650PROSITE-ProRule annotation
Glycosylationi620 – 6201C-linked (Man); partial1 Publication
Glycosylationi623 – 6231C-linked (Man)1 Publication
Disulfide bondi626 ↔ 630PROSITE-ProRule annotation
Disulfide bondi660 ↔ 665PROSITE-ProRule annotation
Glycosylationi674 – 6741C-linked (Man)1 Publication
Glycosylationi681 – 6811N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PeptideAtlasiQ9HCB6.
PRIDEiQ9HCB6.

PTM databases

iPTMnetiQ9HCB6.
PhosphoSiteiQ9HCB6.

Expressioni

Tissue specificityi

Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.2 Publications

Gene expression databases

BgeeiENSG00000262655.
CleanExiHS_SPON1.
GenevisibleiQ9HCB6. HS.

Interactioni

Subunit structurei

Binds to the central extracellular domain of APP and inhibits beta-secretase cleavage of APP.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
APPP05067-43EBI-2431846,EBI-302641

Protein-protein interaction databases

IntActiQ9HCB6. 4 interactions.

Structurei

Secondary structure

1
807
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni43 – 464Combined sources
Turni48 – 503Combined sources
Beta strandi60 – 656Combined sources
Beta strandi70 – 723Combined sources
Beta strandi77 – 848Combined sources
Beta strandi90 – 9910Combined sources
Beta strandi104 – 1063Combined sources
Helixi107 – 1093Combined sources
Beta strandi112 – 1176Combined sources
Turni119 – 1213Combined sources
Beta strandi122 – 1254Combined sources
Beta strandi128 – 1369Combined sources
Beta strandi140 – 1489Combined sources
Beta strandi157 – 16913Combined sources
Beta strandi173 – 1753Combined sources
Beta strandi178 – 1814Combined sources
Beta strandi203 – 21311Combined sources
Turni217 – 2193Combined sources
Turni226 – 2283Combined sources
Beta strandi234 – 2396Combined sources
Helixi254 – 2629Combined sources
Helixi266 – 2738Combined sources
Helixi274 – 2785Combined sources
Beta strandi279 – 2857Combined sources
Beta strandi288 – 2914Combined sources
Turni295 – 2973Combined sources
Beta strandi302 – 31110Combined sources
Beta strandi313 – 32210Combined sources
Beta strandi324 – 33411Combined sources
Beta strandi340 – 3423Combined sources
Beta strandi344 – 3507Combined sources
Beta strandi356 – 3583Combined sources
Beta strandi401 – 41212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZOTX-ray2.70A/B/C/D29-198[»]
2ZOUX-ray1.45A/B40-186[»]
3COOX-ray2.00A/B29-194[»]
3Q13X-ray1.95A191-434[»]
ProteinModelPortaliQ9HCB6.
SMRiQ9HCB6. Positions 44-183, 192-427, 441-499, 618-720.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HCB6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 194166ReelinPROSITE-ProRule annotationAdd
BLAST
Domaini195 – 388194SpondinPROSITE-ProRule annotationAdd
BLAST
Domaini442 – 49554TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini501 – 55555TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini558 – 61154TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini614 – 66653TSP type-1 4PROSITE-ProRule annotationAdd
BLAST
Domaini668 – 72154TSP type-1 5PROSITE-ProRule annotationAdd
BLAST
Domaini754 – 80653TSP type-1 6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 reelin domain.PROSITE-ProRule annotation
Contains 1 spondin domain.PROSITE-ProRule annotation
Contains 6 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00810000125397.
HOGENOMiHOG000124667.
HOVERGENiHBG052920.
InParanoidiQ9HCB6.
OMAiKQFPDDG.
OrthoDBiEOG091G0FDA.
PhylomeDBiQ9HCB6.

Family and domain databases

CDDicd08544. Reeler. 1 hit.
InterProiIPR002861. Reeler_dom.
IPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF02014. Reeler. 1 hit.
PF06468. Spond_N. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 6 hits.
PROSITEiPS51019. REELIN. 1 hit.
PS51020. SPONDIN. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HCB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSPAPLKL SRTPALLALA LPLAAALAFS DETLDKVPKS EGYCSRILRA
60 70 80 90 100
QGTRREGYTE FSLRVEGDPD FYKPGTSYRV TLSAAPPSYF RGFTLIALRE
110 120 130 140 150
NREGDKEEDH AGTFQIIDEE ETQFMSNCPV AVTESTPRRR TRIQVFWIAP
160 170 180 190 200
PAGTGCVILK ASIVQKRIIY FQDEGSLTKK LCEQDSTFDG VTDKPILDCC
210 220 230 240 250
ACGTAKYRLT FYGNWSEKTH PKDYPRRANH WSAIIGGSHS KNYVLWEYGG
260 270 280 290 300
YASEGVKQVA ELGSPVKMEE EIRQQSDEVL TVIKAKAQWP AWQPLNVRAA
310 320 330 340 350
PSAEFSVDRT RHLMSFLTMM GPSPDWNVGL SAEDLCTKEC GWVQKVVQDL
360 370 380 390 400
IPWDAGTDSG VTYESPNKPT IPQEKIRPLT SLDHPQSPFY DPEGGSITQV
410 420 430 440 450
ARVVIERIAR KGEQCNIVPD NVDDIVADLA PEEKDEDDTP ETCIYSNWSP
460 470 480 490 500
WSACSSSTCD KGKRMRQRML KAQLDLSVPC PDTQDFQPCM GPGCSDEDGS
510 520 530 540 550
TCTMSEWITW SPCSISCGMG MRSRERYVKQ FPEDGSVCTL PTEETEKCTV
560 570 580 590 600
NEECSPSSCL MTEWGEWDEC SATCGMGMKK RHRMIKMNPA DGSMCKAETS
610 620 630 640 650
QAEKCMMPEC HTIPCLLSPW SEWSDCSVTC GKGMRTRQRM LKSLAELGDC
660 670 680 690 700
NEDLEQVEKC MLPECPIDCE LTEWSQWSEC NKSCGKGHVI RTRMIQMEPQ
710 720 730 740 750
FGGAPCPETV QRKKCRIRKC LRNPSIQKLR WREARESRRS EQLKEESEGE
760 770 780 790 800
QFPGCRMRPW TAWSECTKLC GGGIQERYMT VKKRFKSSQF TSCKDKKEIR

ACNVHPC
Length:807
Mass (Da):90,973
Last modified:September 27, 2004 - v2
Checksum:iCEA84A83F206A5A5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti500 – 5001S → P in BAC11217 (PubMed:14702039).Curated
Sequence conflicti545 – 5451T → M in BAB18461 (PubMed:11368520).Curated
Sequence conflicti570 – 5701C → Y in BAC11217 (PubMed:14702039).Curated
Sequence conflicti605 – 6051C → R in BAC11217 (PubMed:14702039).Curated
Sequence conflicti695 – 6951I → T in AAH19825 (PubMed:15489334).Curated
Sequence conflicti729 – 7291L → P in BAB18461 (PubMed:11368520).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051390 mRNA. Translation: BAB18461.1.
AK074803 mRNA. Translation: BAC11217.1.
AK291780 mRNA. Translation: BAF84469.1.
CH471064 Genomic DNA. Translation: EAW68488.1.
CH471064 Genomic DNA. Translation: EAW68489.1.
BC019825 mRNA. Translation: AAH19825.1.
BC136513 mRNA. Translation: AAI36514.1.
BC136563 mRNA. Translation: AAI36564.1.
AB018305 mRNA. Translation: BAA34482.2.
CCDSiCCDS73262.1.
RefSeqiNP_006099.2. NM_006108.3.
UniGeneiHs.643864.

Genome annotation databases

EnsembliENST00000576479; ENSP00000460236; ENSG00000262655.
GeneIDi10418.
KEGGihsa:10418.
UCSCiuc031xfv.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051390 mRNA. Translation: BAB18461.1.
AK074803 mRNA. Translation: BAC11217.1.
AK291780 mRNA. Translation: BAF84469.1.
CH471064 Genomic DNA. Translation: EAW68488.1.
CH471064 Genomic DNA. Translation: EAW68489.1.
BC019825 mRNA. Translation: AAH19825.1.
BC136513 mRNA. Translation: AAI36514.1.
BC136563 mRNA. Translation: AAI36564.1.
AB018305 mRNA. Translation: BAA34482.2.
CCDSiCCDS73262.1.
RefSeqiNP_006099.2. NM_006108.3.
UniGeneiHs.643864.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZOTX-ray2.70A/B/C/D29-198[»]
2ZOUX-ray1.45A/B40-186[»]
3COOX-ray2.00A/B29-194[»]
3Q13X-ray1.95A191-434[»]
ProteinModelPortaliQ9HCB6.
SMRiQ9HCB6. Positions 44-183, 192-427, 441-499, 618-720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9HCB6. 4 interactions.

PTM databases

iPTMnetiQ9HCB6.
PhosphoSiteiQ9HCB6.

Polymorphism and mutation databases

BioMutaiSPON1.
DMDMi52783472.

Proteomic databases

PeptideAtlasiQ9HCB6.
PRIDEiQ9HCB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000576479; ENSP00000460236; ENSG00000262655.
GeneIDi10418.
KEGGihsa:10418.
UCSCiuc031xfv.2. human.

Organism-specific databases

CTDi10418.
GeneCardsiSPON1.
H-InvDBHIX0009464.
HGNCiHGNC:11252. SPON1.
MIMi604989. gene.
neXtProtiNX_Q9HCB6.
PharmGKBiPA36082.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00810000125397.
HOGENOMiHOG000124667.
HOVERGENiHBG052920.
InParanoidiQ9HCB6.
OMAiKQFPDDG.
OrthoDBiEOG091G0FDA.
PhylomeDBiQ9HCB6.

Enzyme and pathway databases

ReactomeiR-HSA-5083635. Defective B3GALTL causes Peters-plus syndrome (PpS).
R-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Miscellaneous databases

EvolutionaryTraceiQ9HCB6.
GeneWikiiSpondin_1.
GenomeRNAii10418.
PROiQ9HCB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000262655.
CleanExiHS_SPON1.
GenevisibleiQ9HCB6. HS.

Family and domain databases

CDDicd08544. Reeler. 1 hit.
InterProiIPR002861. Reeler_dom.
IPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF02014. Reeler. 1 hit.
PF06468. Spond_N. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 6 hits.
PROSITEiPS51019. REELIN. 1 hit.
PS51020. SPONDIN. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPON1_HUMAN
AccessioniPrimary (citable) accession number: Q9HCB6
Secondary accession number(s): A8K6W5
, O94862, Q8NCD7, Q8WUR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.