Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Spondin-1

Gene

SPON1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi325CalciumCombined sources1 Publication1
Metal bindingi354CalciumCombined sources1 Publication1
Metal bindingi358Calcium; via carbonyl oxygenCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-5083635. Defective B3GALTL causes Peters-plus syndrome (PpS).
R-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Spondin-1
Alternative name(s):
F-spondin
Vascular smooth muscle cell growth-promoting factor
Gene namesi
Name:SPON1
Synonyms:KIAA0762, VSGP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11252. SPON1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi10418.
OpenTargetsiENSG00000262655.
PharmGKBiPA36082.

Polymorphism and mutation databases

BioMutaiSPON1.
DMDMi52783472.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000003586529 – 807Spondin-1Add BLAST779

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44 ↔ 128Combined sources2 Publications
Disulfide bondi156 ↔ 182Combined sources2 Publications
Disulfide bondi199 ↔ 336Combined sources1 Publication
Disulfide bondi200 ↔ 340Combined sources1 Publication
Disulfide bondi202 ↔ 415Combined sources1 Publication
Glycosylationi214N-linked (GlcNAc...)Combined sources1 Publication1
Disulfide bondi443 ↔ 480PROSITE-ProRule annotation
Glycosylationi448C-linked (Man)1 Publication1
Glycosylationi451C-linked (Man); partial1 Publication1
Disulfide bondi454 ↔ 489PROSITE-ProRule annotation
Disulfide bondi459 ↔ 494PROSITE-ProRule annotation
Disulfide bondi502 ↔ 538PROSITE-ProRule annotation
Glycosylationi507C-linked (Man)1 Publication1
Glycosylationi510C-linked (Man); partial1 Publication1
Disulfide bondi513 ↔ 517PROSITE-ProRule annotation
Disulfide bondi548 ↔ 554PROSITE-ProRule annotation
Disulfide bondi559 ↔ 595PROSITE-ProRule annotation
Glycosylationi564C-linked (Man)1 Publication1
Disulfide bondi570 ↔ 574PROSITE-ProRule annotation
Disulfide bondi605 ↔ 610PROSITE-ProRule annotation
Disulfide bondi615 ↔ 650PROSITE-ProRule annotation
Glycosylationi620C-linked (Man); partial1 Publication1
Glycosylationi623C-linked (Man)1 Publication1
Disulfide bondi626 ↔ 630PROSITE-ProRule annotation
Disulfide bondi660 ↔ 665PROSITE-ProRule annotation
Glycosylationi674C-linked (Man)1 Publication1
Glycosylationi681N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PeptideAtlasiQ9HCB6.
PRIDEiQ9HCB6.

PTM databases

iPTMnetiQ9HCB6.
PhosphoSitePlusiQ9HCB6.

Expressioni

Tissue specificityi

Highest expression in lung, lower expression in brain, heart, kidney, liver and testis, and lowest expression in pancreas, skeletal muscle and ovary. Not expressed in spleen.2 Publications

Gene expression databases

BgeeiENSG00000262655.
CleanExiHS_SPON1.
GenevisibleiQ9HCB6. HS.

Interactioni

Subunit structurei

Binds to the central extracellular domain of APP and inhibits beta-secretase cleavage of APP.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
APPP05067-43EBI-2431846,EBI-302641

Protein-protein interaction databases

IntActiQ9HCB6. 6 interactors.

Structurei

Secondary structure

1807
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni43 – 46Combined sources4
Turni48 – 50Combined sources3
Beta strandi60 – 65Combined sources6
Beta strandi70 – 72Combined sources3
Beta strandi77 – 84Combined sources8
Beta strandi90 – 99Combined sources10
Beta strandi104 – 106Combined sources3
Helixi107 – 109Combined sources3
Beta strandi112 – 117Combined sources6
Turni119 – 121Combined sources3
Beta strandi122 – 125Combined sources4
Beta strandi128 – 136Combined sources9
Beta strandi140 – 148Combined sources9
Beta strandi157 – 169Combined sources13
Beta strandi173 – 175Combined sources3
Beta strandi178 – 181Combined sources4
Beta strandi203 – 213Combined sources11
Turni217 – 219Combined sources3
Turni226 – 228Combined sources3
Beta strandi234 – 239Combined sources6
Helixi254 – 262Combined sources9
Helixi266 – 273Combined sources8
Helixi274 – 278Combined sources5
Beta strandi279 – 285Combined sources7
Beta strandi288 – 291Combined sources4
Turni295 – 297Combined sources3
Beta strandi302 – 311Combined sources10
Beta strandi313 – 322Combined sources10
Beta strandi324 – 334Combined sources11
Beta strandi340 – 342Combined sources3
Beta strandi344 – 350Combined sources7
Beta strandi356 – 358Combined sources3
Beta strandi401 – 412Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZOTX-ray2.70A/B/C/D29-198[»]
2ZOUX-ray1.45A/B40-186[»]
3COOX-ray2.00A/B29-194[»]
3Q13X-ray1.95A191-434[»]
ProteinModelPortaliQ9HCB6.
SMRiQ9HCB6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HCB6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 194ReelinPROSITE-ProRule annotationAdd BLAST166
Domaini195 – 388SpondinPROSITE-ProRule annotationAdd BLAST194
Domaini442 – 495TSP type-1 1PROSITE-ProRule annotationAdd BLAST54
Domaini501 – 555TSP type-1 2PROSITE-ProRule annotationAdd BLAST55
Domaini558 – 611TSP type-1 3PROSITE-ProRule annotationAdd BLAST54
Domaini614 – 666TSP type-1 4PROSITE-ProRule annotationAdd BLAST53
Domaini668 – 721TSP type-1 5PROSITE-ProRule annotationAdd BLAST54
Domaini754 – 806TSP type-1 6PROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Contains 1 reelin domain.PROSITE-ProRule annotation
Contains 1 spondin domain.PROSITE-ProRule annotation
Contains 6 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00810000125397.
HOGENOMiHOG000124667.
HOVERGENiHBG052920.
InParanoidiQ9HCB6.
OMAiKQFPDDG.
OrthoDBiEOG091G0FDA.
PhylomeDBiQ9HCB6.

Family and domain databases

CDDicd08544. Reeler. 1 hit.
InterProiIPR002861. Reeler_dom.
IPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF02014. Reeler. 1 hit.
PF06468. Spond_N. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 6 hits.
PROSITEiPS51019. REELIN. 1 hit.
PS51020. SPONDIN. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HCB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLSPAPLKL SRTPALLALA LPLAAALAFS DETLDKVPKS EGYCSRILRA
60 70 80 90 100
QGTRREGYTE FSLRVEGDPD FYKPGTSYRV TLSAAPPSYF RGFTLIALRE
110 120 130 140 150
NREGDKEEDH AGTFQIIDEE ETQFMSNCPV AVTESTPRRR TRIQVFWIAP
160 170 180 190 200
PAGTGCVILK ASIVQKRIIY FQDEGSLTKK LCEQDSTFDG VTDKPILDCC
210 220 230 240 250
ACGTAKYRLT FYGNWSEKTH PKDYPRRANH WSAIIGGSHS KNYVLWEYGG
260 270 280 290 300
YASEGVKQVA ELGSPVKMEE EIRQQSDEVL TVIKAKAQWP AWQPLNVRAA
310 320 330 340 350
PSAEFSVDRT RHLMSFLTMM GPSPDWNVGL SAEDLCTKEC GWVQKVVQDL
360 370 380 390 400
IPWDAGTDSG VTYESPNKPT IPQEKIRPLT SLDHPQSPFY DPEGGSITQV
410 420 430 440 450
ARVVIERIAR KGEQCNIVPD NVDDIVADLA PEEKDEDDTP ETCIYSNWSP
460 470 480 490 500
WSACSSSTCD KGKRMRQRML KAQLDLSVPC PDTQDFQPCM GPGCSDEDGS
510 520 530 540 550
TCTMSEWITW SPCSISCGMG MRSRERYVKQ FPEDGSVCTL PTEETEKCTV
560 570 580 590 600
NEECSPSSCL MTEWGEWDEC SATCGMGMKK RHRMIKMNPA DGSMCKAETS
610 620 630 640 650
QAEKCMMPEC HTIPCLLSPW SEWSDCSVTC GKGMRTRQRM LKSLAELGDC
660 670 680 690 700
NEDLEQVEKC MLPECPIDCE LTEWSQWSEC NKSCGKGHVI RTRMIQMEPQ
710 720 730 740 750
FGGAPCPETV QRKKCRIRKC LRNPSIQKLR WREARESRRS EQLKEESEGE
760 770 780 790 800
QFPGCRMRPW TAWSECTKLC GGGIQERYMT VKKRFKSSQF TSCKDKKEIR

ACNVHPC
Length:807
Mass (Da):90,973
Last modified:September 27, 2004 - v2
Checksum:iCEA84A83F206A5A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti500S → P in BAC11217 (PubMed:14702039).Curated1
Sequence conflicti545T → M in BAB18461 (PubMed:11368520).Curated1
Sequence conflicti570C → Y in BAC11217 (PubMed:14702039).Curated1
Sequence conflicti605C → R in BAC11217 (PubMed:14702039).Curated1
Sequence conflicti695I → T in AAH19825 (PubMed:15489334).Curated1
Sequence conflicti729L → P in BAB18461 (PubMed:11368520).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051390 mRNA. Translation: BAB18461.1.
AK074803 mRNA. Translation: BAC11217.1.
AK291780 mRNA. Translation: BAF84469.1.
CH471064 Genomic DNA. Translation: EAW68488.1.
CH471064 Genomic DNA. Translation: EAW68489.1.
BC019825 mRNA. Translation: AAH19825.1.
BC136513 mRNA. Translation: AAI36514.1.
BC136563 mRNA. Translation: AAI36564.1.
AB018305 mRNA. Translation: BAA34482.2.
CCDSiCCDS73262.1.
RefSeqiNP_006099.2. NM_006108.3.
UniGeneiHs.643864.

Genome annotation databases

EnsembliENST00000576479; ENSP00000460236; ENSG00000262655.
GeneIDi10418.
KEGGihsa:10418.
UCSCiuc031xfv.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051390 mRNA. Translation: BAB18461.1.
AK074803 mRNA. Translation: BAC11217.1.
AK291780 mRNA. Translation: BAF84469.1.
CH471064 Genomic DNA. Translation: EAW68488.1.
CH471064 Genomic DNA. Translation: EAW68489.1.
BC019825 mRNA. Translation: AAH19825.1.
BC136513 mRNA. Translation: AAI36514.1.
BC136563 mRNA. Translation: AAI36564.1.
AB018305 mRNA. Translation: BAA34482.2.
CCDSiCCDS73262.1.
RefSeqiNP_006099.2. NM_006108.3.
UniGeneiHs.643864.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZOTX-ray2.70A/B/C/D29-198[»]
2ZOUX-ray1.45A/B40-186[»]
3COOX-ray2.00A/B29-194[»]
3Q13X-ray1.95A191-434[»]
ProteinModelPortaliQ9HCB6.
SMRiQ9HCB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9HCB6. 6 interactors.

PTM databases

iPTMnetiQ9HCB6.
PhosphoSitePlusiQ9HCB6.

Polymorphism and mutation databases

BioMutaiSPON1.
DMDMi52783472.

Proteomic databases

PeptideAtlasiQ9HCB6.
PRIDEiQ9HCB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000576479; ENSP00000460236; ENSG00000262655.
GeneIDi10418.
KEGGihsa:10418.
UCSCiuc031xfv.2. human.

Organism-specific databases

CTDi10418.
DisGeNETi10418.
GeneCardsiSPON1.
H-InvDBHIX0009464.
HGNCiHGNC:11252. SPON1.
MIMi604989. gene.
neXtProtiNX_Q9HCB6.
OpenTargetsiENSG00000262655.
PharmGKBiPA36082.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00810000125397.
HOGENOMiHOG000124667.
HOVERGENiHBG052920.
InParanoidiQ9HCB6.
OMAiKQFPDDG.
OrthoDBiEOG091G0FDA.
PhylomeDBiQ9HCB6.

Enzyme and pathway databases

ReactomeiR-HSA-5083635. Defective B3GALTL causes Peters-plus syndrome (PpS).
R-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Miscellaneous databases

EvolutionaryTraceiQ9HCB6.
GeneWikiiSpondin_1.
GenomeRNAii10418.
PROiQ9HCB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000262655.
CleanExiHS_SPON1.
GenevisibleiQ9HCB6. HS.

Family and domain databases

CDDicd08544. Reeler. 1 hit.
InterProiIPR002861. Reeler_dom.
IPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF02014. Reeler. 1 hit.
PF06468. Spond_N. 1 hit.
PF00090. TSP_1. 6 hits.
[Graphical view]
SMARTiSM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 6 hits.
PROSITEiPS51019. REELIN. 1 hit.
PS51020. SPONDIN. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPON1_HUMAN
AccessioniPrimary (citable) accession number: Q9HCB6
Secondary accession number(s): A8K6W5
, O94862, Q8NCD7, Q8WUR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: September 27, 2004
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.