Reviewed, UniProtKB/Swiss-Prot Q9HC98 (NEK6_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase Nek6
    EC=2.7.11.1
Alternative name(s):
    Never in mitosis A-related kinase 6
      Short name=NimA-related protein kinase 6
    Protein kinase SID6-1512

Gene names

Name:

NEK6

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Ref.2 Ref.9
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Subcellular location	Cytoplasm. Nucleus Ref.2.
Tissue specificity	Ubiquitous, with highest expression in heart and skeletal muscle. Ref.2
Post-translational modification	Autophosphorylated. Ref.9 Ref.11
Sequence similarities	Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAH70247.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI10876.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAW87581.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle Cell division Chromosome partition Mitosis
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Ref.9 Inferred from direct assay. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from mutant phenotype. Source: UniProtKB protein amino acid phosphorylation Ref.9 Inferred from direct assay. Source: UniProtKB regulation of mitotic metaphase/anaphase transition Ref.9 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: UniProtKB nucleus Ref.2 Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Ref.9 Inferred from direct assay. Source: UniProtKB magnesium ion binding Ref.9 Inferred from direct assay. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Ref.9 Inferred from direct assay. Source: UniProtKB signal transducer activity Inferred from mutant phenotype. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC37	Q16543	1	EBI-740364,EBI-295634
DYNLL1	P63167	1	EBI-740364,EBI-349105
EML2	O95834	1	EBI-740364,EBI-1054588
EML3	Q32P44	1	EBI-740364,EBI-1046713
EML4	Q9HC35	1	EBI-740364,EBI-717876
FANCI	Q9NVI1	1	EBI-740364,EBI-1013291
IPO4	Q8TEX9	1	EBI-740364,EBI-395967
KDELR1	P24390	1	EBI-740364,EBI-1043076
KIAA1967	Q8N163	1	EBI-740364,EBI-355410
LRPPRC	P42704	1	EBI-740364,EBI-1050853
MCM7	P33993	1	EBI-740364,EBI-355924
MYO1C	O00159	1	EBI-740364,EBI-350423
NEK7	Q8TDX7	1	EBI-740364,EBI-1055945
NEK9	Q8TD19	1	EBI-740364,EBI-1044009
NUP93	Q8N1F7	1	EBI-740364,EBI-1042703
PCID2	Q5JVF3	1	EBI-740364,EBI-1051701
PIN1	Q13526	2	EBI-740364,EBI-714158
PSMD2	Q13200	1	EBI-740364,EBI-357648
PTPLAD1	Q9P035	1	EBI-740364,EBI-359013
RUVBL2	Q9Y230	1	EBI-740364,EBI-352939
SLC1A5	Q15758	1	EBI-740364,EBI-356576
SMC3	Q9UQE7	1	EBI-740364,EBI-80718
USP9X	Q93008	1	EBI-740364,EBI-302524
XPO5	Q9HAV4	1	EBI-740364,EBI-517949

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Serine/threonine-protein kinase Nek6		PRO_0000086427
Regions
	Domain	45 – 310	266	Protein kinase
	Nucleotide binding	51 – 59	9	ATP By similarity
Sites
	Active site	172	1	Proton acceptor By similarity
	Binding site	74	1	ATP By similarity
Amino acid modifications
	Modified residue	35	1	Phosphothreonine
	Modified residue	198	1	Phosphoserine
	Modified residue	199	1	Phosphoserine
	Modified residue	201	1	Phosphothreonine
	Modified residue	202	1	Phosphothreonine
	Modified residue	206	1	Phosphoserine Ref.11
Experimental info
	Mutagenesis	74	1	K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-75. Ref.9
	Mutagenesis	75	1	K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-74. Ref.9
	Sequence conflict	88	1	Q → W in CAG33372. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9HC98-1 [UniParc]. Last modified October 3, 2003. Version 2. Checksum: B898DC57407A8C16	FASTA	313	35,714
	10 20 30 40 50 60 MAGQPGHMPH GGSSNNLCHT LGPVHPPDPQ RHPNTLSFRC SLADFQIEKK IGRGQFSEVY 70 80 90 100 110 120 KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL NHPNIIKYLD SFIEDNELNI 130 140 150 160 170 180 VLELADAGDL SQMIKYFKKQ KRLIPERTVW KYFVQLCSAV EHMHSRRVMH RDIKPANVFI 190 200 210 220 230 240 TATGVVKLGD LGLGRFFSSE TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM 250 260 270 280 290 300 AALQSPFYGD KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCICPDP HQRPDIGYVH 310 QVAKQMHIWM SST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assignment of NEK6, a NIMA-related gene, to human chromosome 9q33. 3-->q34.11 by radiation hybrid mapping." Li M.Z., Yu L., Liu Q., Chu J.Y., Zhao S.Y. Cytogenet. Cell Genet. 87:271-272(1999) [PubMed: 10702691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase." Hashimoto Y., Akita H., Hibino M., Kohri K., Nakanishi M. Biochem. Biophys. Res. Commun. 293:753-758(2002) [PubMed: 12054534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
[3]	"A protein kinase weakly similar to nek1." Saito T., Saito R., Saito S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Pancreas.
[8]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-313.
[9]	"The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis." Yin M.J., Shao L., Voehringer D., Smeal T., Jallal B. J. Biol. Chem. 278:52454-52460(2003) [PubMed: 14563848] [Abstract] Cited for: FUNCTION, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-74 AND LYS-75.
[10]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-35; SER-198; SER-199; THR-201; THR-202 AND SER-206, MASS SPECTROMETRY.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF087909 mRNA. Translation: AAG13417.1. Different initiation. AB026289 mRNA. Translation: BAA85045.1. Different initiation. AK313071 mRNA. Translation: BAG35899.1. AL162724, AL137846 Genomic DNA. Translation: CAH70247.1. Sequence problems. AL162724, AL137846 Genomic DNA. Translation: CAH70254.1. AL137846, AL162724 Genomic DNA. Translation: CAI10876.1. Sequence problems. AL137846, AL162724 Genomic DNA. Translation: CAI10883.1. CH471090 Genomic DNA. Translation: EAW87579.1. CH471090 Genomic DNA. Translation: EAW87581.1. Sequence problems. BC000101 mRNA. Translation: AAH00101.2. BC004174 mRNA. Translation: AAH04174.2. BC004209 mRNA. Translation: AAH04209.2. BC012761 mRNA. Translation: AAH12761.1. CR457091 mRNA. Translation: CAG33372.1. CR542222 mRNA. Translation: CAG47018.1.
IPI	IPI00550738.
PIR	JC7838.
RefSeq	NP_001138473.1. NP_001159639.1. NP_001159640.1. NP_001159641.1. NP_001159642.1. NP_001159643.1. NP_055212.2.
UniGene	Hs.197071
3D structure databases
SMR	Q9HC98. Positions 42-299.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HC98. 44 interactions.
STRING	Q9HC98.
PTM databases
PhosphoSite	Q9HC98.
Proteomic databases
PRIDE	Q9HC98.
Genome annotation databases
Ensembl	ENST00000320246; ENSP00000319734; ENSG00000119408; Homo sapiens. [Genome view] ENST00000373600; ENSP00000362702; ENSG00000119408; Homo sapiens. [Genome view] ENST00000373603; ENSP00000362705; ENSG00000119408; Homo sapiens. [Genome view]
GeneID	10783.
KEGG	hsa:10783.
UCSC	uc004bog.1. human.
Organism-specific databases
CTD	10783.
GeneCards	GC09P126059.
H-InvDB	HIX0008368.
HGNC	HGNC:7749. NEK6.
MIM	604884. gene.
PharmGKB	PA31550.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11865.
HOVERGEN	Q9HC98.
InParanoid	Q9HC98.
OrthoDB	EOG9QJV72.
PhylomeDB	Q9HC98.
Enzyme and pathway databases
BRENDA	2.7.11.1. 247.
Gene expression databases
ArrayExpress	Q9HC98.
Bgee	Q9HC98.
CleanEx	HS_NEK6.
Genevestigator	Q9HC98.
GermOnline	ENSG00000119408. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	40956.
SOURCE	Search...

Entry information

Entry name

NEK6_HUMAN

Accession

Primary (citable) accession number: Q9HC98
Secondary accession number(s): Q5TBG3 , Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9ULX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	October 3, 2003
Last modified:	February 9, 2010
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.