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Reviewed, UniProtKB/Swiss-Prot Q9HC98 (NEK6_HUMAN)

Last modified February 9, 2010. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase Nek6
    EC=2.7.11.1
Alternative name(s):
    Never in mitosis A-related kinase 6
      Short name=NimA-related protein kinase 6
    Protein kinase SID6-1512
Gene names
Name: NEK6
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Ref.2 Ref.9

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Subcellular location

Cytoplasm. Nucleus Ref.2.

Tissue specificity

Ubiquitous, with highest expression in heart and skeletal muscle. Ref.2

Post-translational modification

Autophosphorylated. Ref.9 Ref.11

Sequence similarities

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence CAH70247.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI10876.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAW87581.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Serine/threonine-protein kinase Nek6
PRO_0000086427

Regions

Domain45 – 310266Protein kinase
Nucleotide binding51 – 599ATP By similarity

Sites

Active site1721Proton acceptor By similarity
Binding site741ATP By similarity

Amino acid modifications

Modified residue351Phosphothreonine
Modified residue1981Phosphoserine
Modified residue1991Phosphoserine
Modified residue2011Phosphothreonine
Modified residue2021Phosphothreonine
Modified residue2061Phosphoserine Ref.11

Experimental info

Mutagenesis741K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-75. Ref.9
Mutagenesis751K → M: Loss of autophosphorylation and of kinase activity and induction of apoptosis; when associated with M-74. Ref.9
Sequence conflict881Q → W in CAG33372. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q9HC98-1 [UniParc].

Last modified October 3, 2003. Version 2.
Checksum: B898DC57407A8C16

FASTA31335,714
        10         20         30         40         50         60 
MAGQPGHMPH GGSSNNLCHT LGPVHPPDPQ RHPNTLSFRC SLADFQIEKK IGRGQFSEVY 

        70         80         90        100        110        120 
KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL NHPNIIKYLD SFIEDNELNI 

       130        140        150        160        170        180 
VLELADAGDL SQMIKYFKKQ KRLIPERTVW KYFVQLCSAV EHMHSRRVMH RDIKPANVFI 

       190        200        210        220        230        240 
TATGVVKLGD LGLGRFFSSE TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM 

       250        260        270        280        290        300 
AALQSPFYGD KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCICPDP HQRPDIGYVH 

       310 
QVAKQMHIWM SST 

« Hide

References

« Hide 'large scale' references
[1]"Assignment of NEK6, a NIMA-related gene, to human chromosome 9q33. 3-->q34.11 by radiation hybrid mapping."
Li M.Z., Yu L., Liu Q., Chu J.Y., Zhao S.Y.
Cytogenet. Cell Genet. 87:271-272(1999) [PubMed: 10702691] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase."
Hashimoto Y., Akita H., Hibino M., Kohri K., Nakanishi M.
Biochem. Biophys. Res. Commun. 293:753-758(2002) [PubMed: 12054534] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
[3]"A protein kinase weakly similar to nek1."
Saito T., Saito R., Saito S.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hippocampus.
[5]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed: 15164053] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney and Pancreas.
[8]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-313.
[9]"The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis."
Yin M.J., Shao L., Voehringer D., Smeal T., Jallal B.
J. Biol. Chem. 278:52454-52460(2003) [PubMed: 14563848] [Abstract]
Cited for: FUNCTION, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-74 AND LYS-75.
[10]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-35; SER-198; SER-199; THR-201; THR-202 AND SER-206, MASS SPECTROMETRY.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, MASS SPECTROMETRY.
Tissue: T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF087909 mRNA. Translation: AAG13417.1. Different initiation.
AB026289 mRNA. Translation: BAA85045.1. Different initiation.
AK313071 mRNA. Translation: BAG35899.1.
AL162724, AL137846 Genomic DNA. Translation: CAH70247.1. Sequence problems.
AL162724, AL137846 Genomic DNA. Translation: CAH70254.1.
AL137846, AL162724 Genomic DNA. Translation: CAI10876.1. Sequence problems.
AL137846, AL162724 Genomic DNA. Translation: CAI10883.1.
CH471090 Genomic DNA. Translation: EAW87579.1.
CH471090 Genomic DNA. Translation: EAW87581.1. Sequence problems.
BC000101 mRNA. Translation: AAH00101.2.
BC004174 mRNA. Translation: AAH04174.2.
BC004209 mRNA. Translation: AAH04209.2.
BC012761 mRNA. Translation: AAH12761.1.
CR457091 mRNA. Translation: CAG33372.1.
CR542222 mRNA. Translation: CAG47018.1.
IPIIPI00550738.
PIRJC7838.
RefSeqNP_001138473.1.
NP_001159639.1.
NP_001159640.1.
NP_001159641.1.
NP_001159642.1.
NP_001159643.1.
NP_055212.2.
UniGeneHs.197071

3D structure databases

SMRQ9HC98. Positions 42-299.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9HC98. 44 interactions.
STRINGQ9HC98.

PTM databases

PhosphoSiteQ9HC98.

Proteomic databases

PRIDEQ9HC98.

Genome annotation databases

EnsemblENST00000320246; ENSP00000319734; ENSG00000119408; Homo sapiens. [Genome view]
ENST00000373600; ENSP00000362702; ENSG00000119408; Homo sapiens. [Genome view]
ENST00000373603; ENSP00000362705; ENSG00000119408; Homo sapiens. [Genome view]
GeneID10783.
KEGGhsa:10783.
UCSCuc004bog.1. human.

Organism-specific databases

CTD10783.
GeneCardsGC09P126059.
H-InvDBHIX0008368.
HGNCHGNC:7749. NEK6.
MIM604884. gene.
PharmGKBPA31550.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG11865.
HOVERGENQ9HC98.
InParanoidQ9HC98.
OrthoDBEOG9QJV72.
PhylomeDBQ9HC98.

Enzyme and pathway databases

BRENDA2.7.11.1. 247.

Gene expression databases

ArrayExpressQ9HC98.
BgeeQ9HC98.
CleanExHS_NEK6.
GenevestigatorQ9HC98.
GermOnlineENSG00000119408. Homo sapiens.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio40956.
SOURCESearch...

Entry information

Entry nameNEK6_HUMAN
AccessionPrimary (citable) accession number: Q9HC98
Secondary accession number(s): Q5TBG3 expand/collapse secondary AC list , Q5TBG9, Q6FG86, Q6IAR3, Q96E83, Q9ULX2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: February 9, 2010
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents