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Q9HC73 (CRLF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytokine receptor-like factor 2
Alternative name(s):
Cytokine receptor-like 2
IL-XR
Thymic stromal lymphopoietin protein receptor
Short name=TSLP receptor
Gene names
Name:CRLF2
Synonyms:CRL2, ILXR, TSLPR
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 By similarity. Implicated in the development of the hematopoietic system. Ref.3

Subunit structure

Heterodimer of CRLF2 and IL7R.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein Probable.

Isoform 2: Secreted Probable.

Tissue specificity

Expressed in heart, skeletal muscle, kidney and adult and fetal liver. Primarily expressed in dendrites and monocytes. Weakly expressed in T-cells.

Induction

Up-regulated in activated peripheral monocytes and THP-1 cells.

Domain

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is important for association with JAKs.

Miscellaneous

The gene coding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

Contains 1 fibronectin type-III domain.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9HC73-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9HC73-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-233: SKQENTCNVT...PPKPKLSKFI → TQSRSVTQAG...PGWSRTCDLR
     234-371: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 371349Cytokine receptor-like factor 2
PRO_0000011041

Regions

Topological domain23 – 231209Extracellular Potential
Transmembrane232 – 25221Helical; Potential
Topological domain253 – 371119Cytoplasmic Potential
Domain118 – 21194Fibronectin type-III
Motif200 – 2045WSXWS motif
Motif261 – 2699Box 1 motif

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation551N-linked (GlcNAc...) Potential
Glycosylation1011N-linked (GlcNAc...) Potential
Glycosylation1691N-linked (GlcNAc...) Potential
Disulfide bond71 ↔ 84 By similarity

Natural variations

Alternative sequence162 – 23372SKQEN…LSKFI → TQSRSVTQAGVQWCDLCLLQ PSPPRFKRFSCLSLPSSWDY RHPPPRLANFCIISRDGVSP CWPGWSRTCDLR in isoform 2.
VSP_008786
Alternative sequence234 – 371138Missing in isoform 2.
VSP_008787

Experimental info

Sequence conflict271Missing in BAB15557. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: DEA53814758B69E4

FASTA37142,013
        10         20         30         40         50         60 
MGRLVLLWGA AVFLLGGWMA LGQGGAAEGV QIQIIYFNLE TVQVTWNASK YSRTNLTFHY 

        70         80         90        100        110        120 
RFNGDEAYDQ CTNYLLQEGH TSGCLLDAEQ RDDILYFSIR NGTHPVFTAS RWMVYYLKPS 

       130        140        150        160        170        180 
SPKHVRFSWH QDAVTVTCSD LSYGDLLYEV QYRSPFDTEW QSKQENTCNV TIEGLDAEKC 

       190        200        210        220        230        240 
YSFWVRVKAM EDVYGPDTYP SDWSEVTCWQ RGEIRDACAE TPTPPKPKLS KFILISSLAI 

       250        260        270        280        290        300 
LLMVSLLLLS LWKLWRVKKF LIPSVPDPKS IFPGLFEIHQ GNFQEWITDT QNVAHLHKMA 

       310        320        330        340        350        360 
GAEQESGPEE PLVVQLAKTE AESPRMLDPQ TEEKEASGGS LQLPHQPLQG GDVVTIGGFT 

       370 
FVMNDRSYVA L 

« Hide

Isoform 2 [UniParc].

Checksum: 6ACA668AF4CAE9D4
Show »

FASTA23326,774

References

« Hide 'large scale' references
[1]"Identification of a novel type I cytokine receptor CRL2 preferentially expressed by human dendritic cells and activated monocytes."
Zhang W., Wang J., Wang Q., Chen G., Zhang J., Chen T., Wan T., Zhang Y., Cao X.
Biochem. Biophys. Res. Commun. 281:878-883(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Dendritic cell.
[2]"Molecular cloning of a human novel type I cytokine receptor related to delta1/TSLPR."
Tonozuka Y., Fujio K., Sugiyama T., Nosaka T., Hirai M., Kitamura T.
Cytogenet. Cell Genet. 93:23-25(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: T-cell.
[3]"Human thymic stromal lymphopoietin preferentially stimulates myeloid cells."
Reche P.A., Soumelis V., Gorman D.M., Clifford T., Liu M.-R., Travis M., Zurawski S.M., Johnston J., Liu Y.-J., Spits H., de Waal Malefyt R., Kastelein R.A., Bazan J.F.
J. Immunol. 167:336-343(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION AS A RECEPTOR FOR TSLP.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF142570 mRNA. Translation: AAG27923.1.
AB052639 mRNA. Translation: BAB60717.1.
AF338733 mRNA. Translation: AAK60618.1.
AK026800 mRNA. Translation: BAB15557.1.
RefSeqNP_001012288.2. NM_001012288.2.
NP_071431.2. NM_022148.3. [Q9HC73-1]
UniGeneHs.287729.

3D structure databases

ProteinModelPortalQ9HC73.
SMRQ9HC73. Positions 31-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000370979.

PTM databases

PhosphoSiteQ9HC73.

Polymorphism databases

DMDM38257768.

Proteomic databases

PaxDbQ9HC73.
PRIDEQ9HC73.

Protocols and materials databases

DNASU64109.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000381566; ENSP00000370978; ENSG00000205755. [Q9HC73-2]
GeneID64109.
KEGGhsa:64109.
UCSCuc004cpk.2. human. [Q9HC73-1]

Organism-specific databases

CTD64109.
GeneCardsGC0XM001274.
HGNCHGNC:14281. CRLF2.
MIM300357. gene.
400023. gene.
neXtProtNX_Q9HC73.
PharmGKBPA26883.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG74051.
HOGENOMHOG000172557.
HOVERGENHBG097013.
InParanoidQ9HC73.
KOK05078.
OMAHMSISIC.
PhylomeDBQ9HC73.

Enzyme and pathway databases

SignaLinkQ9HC73.

Gene expression databases

CleanExHS_CRLF2.
GenevestigatorQ9HC73.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
[Graphical view]
SMARTSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCRLF2. human.
GenomeRNAi64109.
NextBio65960.
PROQ9HC73.
SOURCESearch...

Entry information

Entry nameCRLF2_HUMAN
AccessionPrimary (citable) accession number: Q9HC73
Secondary accession number(s): Q9H5R3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM