Reviewed, UniProtKB/Swiss-Prot Q9HC52 (CBX8_HUMAN)

Names and origin

Protein names

Recommended name:
    Chromobox protein homolog 8
Alternative name(s):
    Polycomb 3 homolog
      Short name=Pc3
      Short name=hPc3
    Rectachrome 1

Gene names

Name:	CBX8
Synonyms:	PC3, RC1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Subunit structure	Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Interacts with MLLT3. Ref.5
Subcellular location	Nucleus.
Sequence similarities	Contains 1 chromo domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin Inferred from electronic annotation. Source: InterPro
Molecular function	chromatin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Chromobox protein homolog 8		PRO_0000080215
Regions
	Domain	11 – 69	59	Chromo
Amino acid modifications
	Modified residue	110	1	Phosphoserine Ref.7 Ref.8
	Modified residue	130	1	Phosphoserine Ref.8
	Modified residue	256	1	Phosphoserine Ref.8
	Modified residue	265	1	Phosphoserine Ref.7 Ref.8
	Modified residue	332	1	Phosphoserine Ref.8
	Modified residue	352	1	Phosphoserine Ref.8
Natural variations
	Natural variant	317	1	G → V: dbSNP rs4889891. Ref.2 Ref.3		VAR_014954

Sequences

	Sequence		Length	Mass (Da)
	Q9HC52-1 [UniParc]. Last modified May 10, 2005. Version 3. Checksum: 651263C85B0ECD93	FASTA	389	43,396
	10 20 30 40 50 60 MELSAVGERV FAAEALLKRR IRKGRMEYLV KWKGWSQKYS TWEPEENILD ARLLAAFEER 70 80 90 100 110 120 EREMELYGPK KRGPKPKTFL LKAQAKAKAK TYEFRSDSAR GIRIPYPGRS PQDLASTSRA 130 140 150 160 170 180 REGLRNMGLS PPASSTSTSS TCRAEAPRDR DRDRDRDRER DRERERERER ERERERERER 190 200 210 220 230 240 GTSRVDDKPS SPGDSSKKRG PKPRKELPDP SQRPLGEPSA GLGEYLKGRK LDDTPSGAGK 250 260 270 280 290 300 FPAGHSVIQL ARRQDSDLVQ CGVTSPSSAE ATGKLAVDTF PARVIKHRAA FLEAKGQGAL 310 320 330 340 350 360 DPNGTRVRHG SGPPSSGGGL YRDMGAQGGR PSLIARIPVA RILGDPEEES WSPSLTNLEK 370 380 VVVTDVTSNF LTVTIKESNT DQGFFKEKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties." Bardos J.I., Saurin A.J., Tissot C., Duprez E., Freemont P.S. J. Biol. Chem. 275:28785-28792(2000) [PubMed: 10825164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of Polycomb and Trithorax-related genes that are expressed in human colorectal mucosa." Michael M.Z., James R.J. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-317. Tissue: Colon carcinoma.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-317. Tissue: Embryo.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Muscle.
[5]	"The ENL moiety of the childhood leukemia-associated MLL-ENL oncoprotein recruits human Polycomb 3." Garcia-Cuellar M.P., Zilles O., Schreiner S.A., Birke M., Winkler T.H., Slany R.K. Oncogene 20:411-419(2001) [PubMed: 11313972] [Abstract] Cited for: INTERACTION WITH MLLT3.
[6]	"The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans." Levine S.S., Weiss A., Erdjument-Bromage H., Shao Z., Tempst P., Kingston R.E. Mol. Cell. Biol. 22:6070-6078(2002) [PubMed: 12167701] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE PRC1 COMPLEX WITH PCGF2; BMI1; CBX2; CBX4; PHC1; PHC2; PHC3; SCMH1; RING1 AND RNF2.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110 AND SER-265, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-130; SER-256; SER-265; SER-332 AND SER-352, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF174482 mRNA. Translation: AAG09180.1. AF266479 mRNA. Translation: AAF76328.2. AK074560 mRNA. Translation: BAC11061.1. BC008937 mRNA. Translation: AAH08937.1. BC009376 mRNA. Translation: AAH09376.1. BC019289 mRNA. Translation: AAH19289.1.
IPI	IPI00307659.
RefSeq	NP_065700.1.
UniGene	Hs.387258
3D structure databases
HSSP	HSSP built from PDB template 1AP0 based on UniProtKB P23197.
SMR	Q9HC52. Positions 7-58.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HC52. 6 interactions.
PTM databases
PhosphoSite	Q9HC52.
Proteomic databases
PeptideAtlas	Q9HC52.
PRIDE	Q9HC52.
Genome annotation databases
Ensembl	ENSG00000141570. Homo sapiens. [Contig view]
GeneID	57332.
KEGG	hsa:57332.
Organism-specific databases
GeneCards	GC17M075382.
H-InvDB	HIX0014234.
HGNC	HGNC:15962. CBX8.
PharmGKB	PA26133.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9HC52.
HOVERGEN	Q9HC52.
OMA	Q9HC52. RRQDSDL.
Gene expression databases
ArrayExpress	Q9HC52.
Bgee	Q9HC52.
CleanEx	HS_CBX8.
GermOnline	ENSG00000141570. Homo sapiens.
Family and domain databases
InterPro	IPR000953. Chromodomain. [Graphical view]
Pfam	PF00385. Chromo. 1 hit. [Graphical view]
SMART	SM00298. CHROMO. 1 hit. [Graphical view]
PROSITE	PS00598. CHROMO_1. 1 hit. PS50013. CHROMO_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	63438.

Entry information

Entry name

CBX8_HUMAN

Accession

Primary (citable) accession number: Q9HC52
Secondary accession number(s): Q96H39, Q9NR07

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 10, 2005
Last modified:	June 16, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)