Q9HC38 (GLOD4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxalase domain-containing protein 4

Gene names

Name:	GLOD4
Synonyms:	C17orf25
ORF Names:	CGI-150, My027

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with NUDT9. Ref.7
Subcellular location	Mitochondrion Probable Ref.7.
Tissue specificity	Expressed in heart, brain, liver, kidney, pancreas and placenta. Not expressed in skeletal muscle and lung. Ref.1
Miscellaneous	Expression is decreased in hepatocellular carcinoma samples as compared to adjacent non-cancerous liver tissues from the same patients. Transfection in hepatocellular carcinoma cells and overexpression can inhibit the cell growth.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9HC38-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9HC38-2)

The sequence of this isoform differs from the canonical sequence as follows:
     32-62: ESCSVARLECSGAISAHCSDYTRITEDSFSK → LRHEEFEEGCKAACNG

Isoform 3 (identifier: Q9HC38-3)

The sequence of this isoform differs from the canonical sequence as follows:
     32-62: ESCSVARLECSGAISAHCSDYTRITEDSFSK → LRHEEFEEGCKAACNG
     197-203: CKLELQG → VSNLGEE
     204-313: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 313

313

Glyoxalase domain-containing protein 4

PRO_0000280391

Natural variations

Alternative sequence

32 – 62

31

ESCSV…DSFSK → LRHEEFEEGCKAACNG in isoform 2 and isoform 3.

VSP_023646

Alternative sequence

197 – 203

7

CKLELQG → VSNLGEE in isoform 3.

VSP_023647

Alternative sequence

204 – 313

110

Missing in isoform 3.

VSP_023648

Natural variant

282

1

M → I. Ref.5
Corresponds to variant rs17851349 [ dbSNP | Ensembl ].

VAR_031128

Experimental info

Sequence conflict

295

1

A → S in AAG17987. Ref.1

Secondary structure

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313

Helix Strand Turn

Details...

Beta strand

5 – 12

8

Helix

16 – 25

10

Beta strand

30 – 37

8

Beta strand

68 – 76

9

Turn

78 – 80

3

Beta strand

83 – 89

7

Beta strand

99 – 107

9

Helix

109 – 118

10

Beta strand

123 – 126

4

Beta strand

129 – 133

5

Beta strand

139 – 145

7

Beta strand

152 – 161

10

Helix

163 – 172

10

Beta strand

177 – 182

6

Turn

183 – 186

4

Beta strand

187 – 193

7

Beta strand

198 – 203

6

Beta strand

216 – 221

6

Helix

223 – 225

3

Helix

226 – 235

10

Beta strand

239 – 247

9

Beta strand

255 – 261

7

Beta strand

267 – 272

6

Helix

273 – 279

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 43DB04159153F07C	FASTA	313	34,793
	10 20 30 40 50 60 MAARRALHFV FKVGNRFQTA RFYRDVLGMK VESCSVARLE CSGAISAHCS DYTRITEDSF 70 80 90 100 110 120 SKPYDGKWSK TMVGFGPEDD HFVAELTYNY GVGDYKLGND FMGITLASSQ AVSNARKLEW 130 140 150 160 170 180 PLTEVAEGVF ETEAPGGYKF YLQNRSLPQS DPVLKVTLAV SDLQKSLNYW CNLLGMKIYE 190 200 210 220 230 240 KDEEKQRALL GYADNQCKLE LQGVKGGVDH AAAFGRIAFS CPQKELPDLE DLMKRENQKI 250 260 270 280 290 300 LTPLVSLDTP GKATVQVVIL ADPDGHEICF VGDEAFRELS KMDPEGSKLL DDAMAADKSD 310 EWFAKHNKPK ASG
	Isoform 2 [UniParc]. Checksum: 92A5D51488DC8C02	FASTA	298	33,233
	10 20 30 40 50 60 MAARRALHFV FKVGNRFQTA RFYRDVLGMK VLRHEEFEEG CKAACNGPYD GKWSKTMVGF 70 80 90 100 110 120 GPEDDHFVAE LTYNYGVGDY KLGNDFMGIT LASSQAVSNA RKLEWPLTEV AEGVFETEAP 130 140 150 160 170 180 GGYKFYLQNR SLPQSDPVLK VTLAVSDLQK SLNYWCNLLG MKIYEKDEEK QRALLGYADN 190 200 210 220 230 240 QCKLELQGVK GGVDHAAAFG RIAFSCPQKE LPDLEDLMKR ENQKILTPLV SLDTPGKATV 250 260 270 280 290 QVVILADPDG HEICFVGDEA FRELSKMDPE GSKLLDDAMA ADKSDEWFAK HNKPKASG
	Isoform 3 [UniParc]. Checksum: D60B2D0ABEF774CE	FASTA	188	21,258
	10 20 30 40 50 60 MAARRALHFV FKVGNRFQTA RFYRDVLGMK VLRHEEFEEG CKAACNGPYD GKWSKTMVGF 70 80 90 100 110 120 GPEDDHFVAE LTYNYGVGDY KLGNDFMGIT LASSQAVSNA RKLEWPLTEV AEGVFETEAP 130 140 150 160 170 180 GGYKFYLQNR SLPQSDPVLK VTLAVSDLQK SLNYWCNLLG MKIYEKDEEK QRALLGYADN QVSNLGEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel gene (C17orf25) from the deletion region on chromosome 17p13.3 in hepatocellular carcinoma." Qin W.X., Wan D.F., Sun F.Y., Zhang P.P., Han L.W., Huang Y., Jiang H.Q., Zhao X.T., He M., Ye Y., Cong W.M., Wu M.C., Zhang L.S., Yang N.W., Gu J.R. Cell Res. 11:209-216(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
[2]	Mao Y.M., Xie Y., Ying K. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Fetal brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ILE-282. Tissue: Brain.
[6]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 6-12; 97-116; 156-177; 188-198; 240-252 AND 289-305, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[7]	"Interaction of C17orf25 with ADP-ribose pyrophosphatase NUDT9 detected via yeast two-hybrid method." Zhang H.-T., Yan Z.-Q., Hu X.-B., Yang S.-L., Gong Y. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 35:747-751(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NUDT9.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF177342 mRNA. Translation: AAG17986.1.
AF177343 mRNA. Translation: AAG17987.1.
AF061730 mRNA. Translation: AAG43141.1.
AK001488 mRNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90645.1.
CH471108 Genomic DNA. Translation: EAW90646.1.
CH471108 Genomic DNA. Translation: EAW90647.1.
CH471108 Genomic DNA. Translation: EAW90649.1.
CH471108 Genomic DNA. Translation: EAW90650.1.
BC008605 mRNA. Translation: AAH08605.1.
BC015848 mRNA. Translation: AAH15848.1.

IPI

IPI00032575.
IPI00745272.
IPI00792035.

RefSeq

NP_057164.3. NM_016080.3.

UniGene

Hs.279061.
Hs.595572.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ZI1	X-ray	1.90	A	1-287	[»]

ProteinModelPortal

Q9HC38.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000301329.

PTM databases

PhosphoSite

Q9HC38.

Polymorphism databases

DMDM

74718831.

2D gel databases

REPRODUCTION-2DPAGE

IPI00792035.

Proteomic databases

PaxDb

Q9HC38.

PRIDE

Q9HC38.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000301328; ENSP00000301328; ENSG00000167699.
ENST00000301329; ENSP00000301329; ENSG00000167699.

GeneID

51031.

KEGG

hsa:51031.

UCSC

uc002frt.3. human.
uc002fru.3. human.

Organism-specific databases

CTD

51031.

GeneCards

GC17M000675.

HGNC

HGNC:14111. GLOD4.

HPA

HPA021623.
HPA023246.
HPA023248.

neXtProt

NX_Q9HC38.

PharmGKB

PA162389795.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0346.

HOGENOM

HOG000006776.

HOVERGEN

HBG056038.

OMA

KTATFYR.

Gene expression databases

ArrayExpress

Q9HC38.

Bgee

Q9HC38.

CleanEx

HS_GLOD4.

Genevestigator

Q9HC38.

Family and domain databases

InterPro

IPR025870. Glyoxalase-like_dom.
[Graphical view]

Pfam

PF12681. Glyoxalase_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

GLOD4. human.

GenomeRNAi

51031.

NextBio

53585.

Entry information

Entry name

GLOD4_HUMAN

Accession

Primary (citable) accession number: Q9HC38
Secondary accession number(s): D3DTG9 , D3DTH1, Q96B89, Q9H3J8, Q9HC37, Q9NVN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.