MRM3

Functionⁱ

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 1370 (Gm1370) in the 16S mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a conserved modification in the peptidyl transferase domain of the mtLSU rRNA.3 Publications

Catalytic activityⁱ

S-adenosyl-L-methionine + guanosine(1370) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(1370) in 16S rRNA.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	356	S-adenosyl-L-methionine; via carbonyl oxygenBy similarity	1
Binding siteⁱ	380	S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity	1
Binding siteⁱ	389	S-adenosyl-L-methionine; via amide nitrogenBy similarity	1

GO - Molecular functionⁱ

Complete GO annotation...

GO - Biological processⁱ

rRNA 2'-O-methylation Source: Reactome

Complete GO annotation...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	rRNA processing
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

Reactomeⁱ	R-HSA-6793080. rRNA modification in the mitochondrion.

Reactomeⁱ

R-HSA-6793080. rRNA modification in the mitochondrion.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: rRNA methyltransferase 3, mitochondrial1 Publication (EC:2.1.1.-2 Publications) Alternative name(s): 16S rRNA (guanosine(1370)-2'-O)-methyltransferase1 Publication 16S rRNA [Gm1370] 2'-O-methyltransferase1 Publication RNA methyltransferase-like protein 11 Publication
Gene namesⁱ	Name:MRM31 PublicationImported Synonyms:RNMTL11 Publication ORF Names:HC90
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 17

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:18485. MRM3.

HGNCⁱ

HGNC:18485. MRM3.

Subcellular locationⁱ

Mitochondrion
2 Publications
Manual assertion based on experiment inⁱ
- Ref.8
  "Mitochondrial ribosomal RNA (rRNA) methyltransferase family members are positioned to modify nascent rRNA in foci near the mitochondrial DNA nucleoid."
  Lee K.W., Okot-Kotber C., LaComb J.F., Bogenhagen D.F.
  J. Biol. Chem. 288:31386-31399(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION.
- Ref.11
  "MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome."
  Rorbach J., Boesch P., Gammage P.A., Nicholls T.J., Pearce S.F., Patel D., Hauser A., Perocchi F., Minczuk M.
  Mol. Biol. Cell 25:2542-2555(2014) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION.

GO - Cellular componentⁱ

mitochondrial matrix Source: Reactome
mitochondrion Source: HPA

Complete GO annotation...

Keywords - Cellular componentⁱ

Mitochondrion

Pathology & Biotechⁱ

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000171861.
PharmGKBⁱ	PA38341.

Polymorphism and mutation databases

BioMutaⁱ	RNMTL1.
DMDMⁱ	74734265.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transit peptideⁱ	1 – 40	MitochondrionSequence analysisAdd BLAST		40
ChainⁱPRO_0000311301	41 – 420	rRNA methyltransferase 3, mitochondrialAdd BLAST		380

Proteomic databases

EPDⁱ	Q9HC36.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9HC36.
PaxDbⁱ	Q9HC36.
PeptideAtlas More...PeptideAtlasⁱ	Q9HC36.
PRIDEⁱ	Q9HC36.

PTM databases

iPTMnetⁱ	Q9HC36.
PhosphoSitePlusⁱ	Q9HC36.

Expressionⁱ

Tissue specificityⁱ

Expressed at same level in normal liver and hepatocarcinoma.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000171861.
CleanExⁱ	HS_RNMTL1.
ExpressionAtlasⁱ	Q9HC36. baseline and differential.
Genevisibleⁱ	Q9HC36. HS.

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA022534. HPA023031. HPA023292.

HPAⁱ

HPA022534.
HPA023031.
HPA023292.

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
FHL3	Q13643	5	EBI-1045440,EBI-741101

With

Entry

#Exp.

IntAct

Notes

FHL3

Q13643

5

EBI-1045440,EBI-741101

Protein-protein interaction databases

BioGridⁱ	120477. 58 interactors.
IntActⁱ	Q9HC36. 8 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-8052394.
STRINGⁱ	9606.ENSP00000306080.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9HC36.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.Curated

Keywords - Domainⁱ

Transit peptide

Phylogenomic databases

eggNOGⁱ	KOG2506. Eukaryota. COG0566. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017317.
HOGENOMⁱ	HOG000154151.
HOVERGENⁱ	HBG108411.
InParanoidⁱ	Q9HC36.
KEGG Orthology (KO) More...KOⁱ	K20095.
OMAⁱ	INNLDWE.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0OPI.
PhylomeDBⁱ	Q9HC36.
TreeFamⁱ	TF323420.

Family and domain databases

Gene3Dⁱ	3.30.1330.30. 1 hit. 3.40.1280.10. 1 hit.
InterProⁱ	View protein in InterPro IPR029028. Alpha/beta_knot_MTases. IPR029064. L30e-like. IPR001537. SpoU_MeTrfase. IPR013123. SpoU_subst-bd. IPR029026. tRNA_m1G_MTases_N.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00588. SpoU_methylase. 1 hit.
SMARTⁱ	View protein in SMART SM00967. SpoU_sub_bind. 1 hit.
SUPFAMⁱ	SSF55315. SSF55315. 1 hit. SSF75217. SSF75217. 2 hits.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9HC36-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAALVRPARF VVRPLLQVVQ AWDLDARRWV RALRRSPVKV VFPSGEVVEQ 
        60         70         80         90        100
KRAPGKQPRK APSEASAQEQ REKQPLEESA SRAPSTWEES GLRYDKAYPG 
       110        120        130        140        150
DRRLSSVMTI VKSRPFREKQ GKILLEGRRL ISDALKAGAV PKMFFFSRLE 
       160        170        180        190        200
YLKELPVDKL KGVSLIKVKF EDIKDWSDLV TPQGIMGIFA KPDHVKMTYP 
       210        220        230        240        250
KTQLQHSLPL LLICDNLRDP GNLGTILRSA AGAGCSKVLL TKGCVDAWEP 
       260        270        280        290        300
KVLRAGMGAH FRMPIINNLE WETVPNYLPP DTRVYVADNC GLYAQAEMSN 
       310        320        330        340        350
KASDHGWVCD QRVMKFHKYE EEEDVETGAS QDWLPHVEVQ SYDSDWTEAP 
       360        370        380        390        400
AAVVIGGETY GVSLESLQLA ESTGGKRLLI PVVPGVDSLN SAMAASILLF 
       410        420
EGKRQLRGRA EDLSRDRSYH

Length:420

Mass (Da):47,020

Last modified:June 1, 2002 - v2

Checksum:ⁱCC06C27489A89FC3

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	247	A → V in BAD96620 (Ref. 3) Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_037217	8	A → S1 PublicationCorresponds to variant dbSNP:rs2273454Ensembl.	1
Natural variantⁱVAR_037218	45	G → E. Corresponds to variant dbSNP:rs2249542Ensembl.	1
Natural variantⁱVAR_037219	185	I → V1 PublicationCorresponds to variant dbSNP:rs17854653Ensembl.	1
Natural variantⁱVAR_037220	326	E → Q. Corresponds to variant dbSNP:rs35780267Ensembl.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK001443 mRNA. Translation: BAA91694.1. AK222900 mRNA. Translation: BAD96620.1. AF177344 mRNA. Translation: AAG17988.2. CH471108 Genomic DNA. Translation: EAW90642.1. BC011550 mRNA. Translation: AAH11550.1. BC050614 mRNA. Translation: AAH50614.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS10997.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001304876.1. NM_001317947.1. NP_060616.1. NM_018146.3.
UniGeneⁱ	Hs.182729.

Genome annotation databases

Ensemblⁱ	ENST00000304478; ENSP00000306080; ENSG00000171861.
GeneIDⁱ	55178.
KEGGⁱ	hsa:55178.
UCSC genome browser More...UCSCⁱ	uc002frw.4. human.

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q9HC36	G3RHL5 UPI0000489AB2	Homo sapiens (Human) Gorilla gorilla gorilla (Western lowland gorilla)	420	UniRef100_Q9HC36	Cluster: rRNA methyltransferase 3, mitochondrial	3

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q9HC36	G3RHL5 UPI0000489AB2 UPI0005332D99 A0A096P290 K7CMU7 G7NHK1 UPI00075FC0D7 I0FT14 H9ZAR1 G7PSY6 +13	Homo sapiens (Human) Gorilla gorilla gorilla (Western lowland gorilla) Rhinopithecus roxellana (Golden snub-nosed monkey) (Pygathrix roxellana) Papio anubis (Olive baboon) Pan troglodytes (Chimpanzee) Macaca mulatta (Rhesus macaque) Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) Nomascus leucogenys (Northern white-cheeked gibbon) (Hylobates leucogenys) And more	420	UniRef90_Q9HC36	Cluster: rRNA methyltransferase 3, mitochondrial	24

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q9HC36	G3RHL5 UPI0000489AB2 UPI0005332D99 A0A096P290 UPI0005F58C37 G7NHK1 UPI00075FC0D7 UPI0005F3876C G7PSY6 A0A0D9RP36 +181	Homo sapiens (Human) Gorilla gorilla gorilla (Western lowland gorilla) Rhinopithecus roxellana (Golden snub-nosed monkey) (Pygathrix roxellana) Papio anubis (Olive baboon) Colobus angolensis palliatus (Peters' Angolan colobus) Macaca mulatta (Rhesus macaque) Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Macaca nemestrina (Pig-tailed macaque) Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) Pan troglodytes (Chimpanzee) And more	420	UniRef50_Q9HC36	Cluster: rRNA methyltransferase 3, mitochondrial	192

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK001443 mRNA. Translation: BAA91694.1. AK222900 mRNA. Translation: BAD96620.1. AF177344 mRNA. Translation: AAG17988.2. CH471108 Genomic DNA. Translation: EAW90642.1. BC011550 mRNA. Translation: AAH11550.1. BC050614 mRNA. Translation: AAH50614.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS10997.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001304876.1. NM_001317947.1. NP_060616.1. NM_018146.3.
UniGeneⁱ	Hs.182729.

3D structure databases

ProteinModelPortalⁱ	Q9HC36.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	120477. 58 interactors.
IntActⁱ	Q9HC36. 8 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-8052394.
STRINGⁱ	9606.ENSP00000306080.

PTM databases

iPTMnetⁱ	Q9HC36.
PhosphoSitePlusⁱ	Q9HC36.

Polymorphism and mutation databases

BioMutaⁱ	RNMTL1.
DMDMⁱ	74734265.

Proteomic databases

EPDⁱ	Q9HC36.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9HC36.
PaxDbⁱ	Q9HC36.
PeptideAtlas More...PeptideAtlasⁱ	Q9HC36.
PRIDEⁱ	Q9HC36.

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	55178.
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000304478; ENSP00000306080; ENSG00000171861.
GeneIDⁱ	55178.
KEGGⁱ	hsa:55178.
UCSC genome browser More...UCSCⁱ	uc002frw.4. human.

Organism-specific databases

CTDⁱ	55178.
GeneCardsⁱ	MRM3.
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:18485. MRM3.
Human Protein Atlas More...HPAⁱ	HPA022534. HPA023031. HPA023292.
MIMⁱ	612600. gene.
neXtProtⁱ	NX_Q9HC36.
Open Targets More...OpenTargetsⁱ	ENSG00000171861.
PharmGKBⁱ	PA38341.
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2506. Eukaryota. COG0566. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017317.
HOGENOMⁱ	HOG000154151.
HOVERGENⁱ	HBG108411.
InParanoidⁱ	Q9HC36.
KEGG Orthology (KO) More...KOⁱ	K20095.
OMAⁱ	INNLDWE.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0OPI.
PhylomeDBⁱ	Q9HC36.
TreeFamⁱ	TF323420.

Enzyme and pathway databases

Reactomeⁱ	R-HSA-6793080. rRNA modification in the mitochondrion.

Reactomeⁱ

R-HSA-6793080. rRNA modification in the mitochondrion.

Miscellaneous databases

ChiTaRSⁱ	RNMTL1. human.
GeneWikiⁱ	RNMTL1.
GenomeRNAiⁱ	55178.
Protein Ontology More...PROⁱ	PR:Q9HC36.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000171861.
CleanExⁱ	HS_RNMTL1.
ExpressionAtlasⁱ	Q9HC36. baseline and differential.
Genevisibleⁱ	Q9HC36. HS.

Family and domain databases

Gene3Dⁱ	3.30.1330.30. 1 hit. 3.40.1280.10. 1 hit.
InterProⁱ	View protein in InterPro IPR029028. Alpha/beta_knot_MTases. IPR029064. L30e-like. IPR001537. SpoU_MeTrfase. IPR013123. SpoU_subst-bd. IPR029026. tRNA_m1G_MTases_N.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00588. SpoU_methylase. 1 hit.
SMARTⁱ	View protein in SMART SM00967. SpoU_sub_bind. 1 hit.
SUPFAMⁱ	SSF55315. SSF55315. 1 hit. SSF75217. SSF75217. 2 hits.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MRM3_HUMAN
Accessionⁱ	Q9HC36Primary (citable) accession number: Q9HC36 Secondary accession number(s): Q53GN1 , Q86VC3, Q96F76, Q9NVQ5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
	Last sequence update:	June 1, 2002
	Last modified:	August 30, 2017
	This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9HC36 (MRM3_HUMAN)

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rRNA methyltransferase 3, mitochondrial

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ