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Protein

Echinoderm microtubule-associated protein-like 4

Gene

EML4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.By similarity

GO - Molecular functioni

GO - Biological processi

  • microtubule-based process Source: UniProtKB
  • microtubule cytoskeleton organization Source: GO_Central
  • mitotic nuclear division Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143924-MONOMER.
SignaLinkiQ9HC35.

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 4
Short name:
EMAP-4
Alternative name(s):
Restrictedly overexpressed proliferation-associated protein
Ropp 120
Gene namesi
Name:EML4
Synonyms:C2orf2, EMAPL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:1316. EML4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi27436.
MalaCardsiEML4.
OpenTargetsiENSG00000143924.
Orphaneti357191. Selection of therapeutic option in non-small cell lung carcinoma.
PharmGKBiPA27769.

Polymorphism and mutation databases

BioMutaiEML4.
DMDMi296439364.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509631 – 981Echinoderm microtubule-associated protein-like 4Add BLAST981

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1
Modified residuei61PhosphoserineCombined sources1
Modified residuei96PhosphothreonineCombined sources1
Modified residuei144PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei200PhosphoserineCombined sources1
Modified residuei201PhosphothreonineCombined sources1
Modified residuei226PhosphotyrosineCombined sources1
Modified residuei237PhosphothreonineCombined sources1
Modified residuei891PhosphoserineCombined sources1
Modified residuei895PhosphoserineCombined sources1
Modified residuei897PhosphothreonineCombined sources1
Modified residuei899PhosphothreonineCombined sources1
Modified residuei903PhosphoserineCombined sources1
Modified residuei978PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9HC35.
MaxQBiQ9HC35.
PaxDbiQ9HC35.
PeptideAtlasiQ9HC35.
PRIDEiQ9HC35.

PTM databases

iPTMnetiQ9HC35.
PhosphoSitePlusiQ9HC35.

Expressioni

Developmental stagei

Strongly overexpressed during mitosis.

Gene expression databases

BgeeiENSG00000143924.
CleanExiHS_EML4.
ExpressionAtlasiQ9HC35. baseline and differential.
GenevisibleiQ9HC35. HS.

Organism-specific databases

HPAiHPA036687.
HPA036688.
HPA065337.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi118169. 22 interactors.
IntActiQ9HC35. 9 interactors.
MINTiMINT-5005640.
STRINGi9606.ENSP00000320663.

Structurei

Secondary structure

1981
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 43Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CGCX-ray2.90A/B/C6-64[»]
ProteinModelPortaliQ9HC35.
SMRiQ9HC35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati307 – 356WD 1Add BLAST50
Repeati361 – 404WD 2Add BLAST44
Repeati407 – 448WD 3Add BLAST42
Repeati454 – 492WD 4Add BLAST39
Repeati507 – 546WD 5Add BLAST40
Repeati590 – 629WD 6Add BLAST40
Repeati673 – 712WD 7Add BLAST40
Repeati719 – 758WD 8Add BLAST40
Repeati832 – 872WD 9Add BLAST41

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410ISA0. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ9HC35.
KOiK15420.
PhylomeDBiQ9HC35.
TreeFamiTF317832.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HC35-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGFAGSLDD SISAASTSDV QDRLSALESR VQQQEDEITV LKAALADVLR
60 70 80 90 100
RLAISEDHVA SVKKSVSSKG QPSPRAVIPM SCITNGSGAN RKPSHTSAVS
110 120 130 140 150
IAGKETLSSA AKSGTEKKKE KPQGQREKKE ESHSNDQSPQ IRASPSPQPS
160 170 180 190 200
SQPLQIHRQT PESKNATPTK SIKRPSPAEK SHNSWENSDD SRNKLSKIPS
210 220 230 240 250
TPKLIPKVTK TADKHKDVII NQEGEYIKMF MRGRPITMFI PSDVDNYDDI
260 270 280 290 300
RTELPPEKLK LEWAYGYRGK DCRANVYLLP TGKIVYFIAS VVVLFNYEER
310 320 330 340 350
TQRHYLGHTD CVKCLAIHPD KIRIATGQIA GVDKDGRPLQ PHVRVWDSVT
360 370 380 390 400
LSTLQIIGLG TFERGVGCLD FSKADSGVHL CIIDDSNEHM LTVWDWQKKA
410 420 430 440 450
KGAEIKTTNE VVLAVEFHPT DANTIITCGK SHIFFWTWSG NSLTRKQGIF
460 470 480 490 500
GKYEKPKFVQ CLAFLGNGDV LTGDSGGVML IWSKTTVEPT PGKGPKGVYQ
510 520 530 540 550
ISKQIKAHDG SVFTLCQMRN GMLLTGGGKD RKIILWDHDL NPEREIEVPD
560 570 580 590 600
QYGTIRAVAE GKADQFLVGT SRNFILRGTF NDGFQIEVQG HTDELWGLAT
610 620 630 640 650
HPFKDLLLTC AQDRQVCLWN SMEHRLEWTR LVDEPGHCAD FHPSGTVVAI
660 670 680 690 700
GTHSGRWFVL DAETRDLVSI HTDGNEQLSV MRYSIDGTFL AVGSHDNFIY
710 720 730 740 750
LYVVSENGRK YSRYGRCTGH SSYITHLDWS PDNKYIMSNS GDYEILYWDI
760 770 780 790 800
PNGCKLIRNR SDCKDIDWTT YTCVLGFQVF GVWPEGSDGT DINALVRSHN
810 820 830 840 850
RKVIAVADDF CKVHLFQYPC SKAKAPSHKY SAHSSHVTNV SFTHNDSHLI
860 870 880 890 900
STGGKDMSII QWKLVEKLSL PQNETVADTT LTKAPVSSTE SVIQSNTPTP
910 920 930 940 950
PPSQPLNETA EEESRISSSP TLLENSLEQT VEPSEDHSEE ESEEGSGDLG
960 970 980
EPLYEEPCNE ISKEQAKATL LEDQQDPSPS S
Length:981
Mass (Da):108,916
Last modified:May 18, 2010 - v3
Checksum:i7E8F2585747736B9
GO
Isoform 2 (identifier: Q9HC35-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-169: Missing.

Note: No experimental confirmation available.
Show »
Length:923
Mass (Da):102,444
Checksum:i82F2C3F43C4C8836
GO

Sequence cautioni

The sequence AAI04648 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA91919 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti768 – 773WTTYTC → SSRPCW in AAY15086 (PubMed:15815621).Curated6

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031726283K → E.3 PublicationsCorresponds to variant rs6736913dbSNPEnsembl.1
Natural variantiVAR_031727382I → V.3 PublicationsCorresponds to variant rs10202624dbSNPEnsembl.1
Natural variantiVAR_031728398K → R.2 PublicationsCorresponds to variant rs28651764dbSNPEnsembl.1
Natural variantiVAR_031729978S → L.Corresponds to variant rs28364731dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047192112 – 169Missing in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177377 mRNA. Translation: AAG09279.2.
AK001804 mRNA. Translation: BAA91919.1. Different initiation.
AK314702 mRNA. Translation: BAG37250.1.
AC006038 Genomic DNA. Translation: AAY15086.1.
AC083949 Genomic DNA. No translation available.
AC096668 Genomic DNA. Translation: AAY14895.1.
BC023522 mRNA. Translation: AAH23522.1.
BC104647 mRNA. Translation: AAI04648.1. Sequence problems.
BC140845 mRNA. Translation: AAI40846.1.
BC146799 mRNA. Translation: AAI46800.1.
CCDSiCCDS1807.1. [Q9HC35-1]
CCDS46266.1. [Q9HC35-2]
RefSeqiNP_001138548.1. NM_001145076.2.
NP_061936.2. NM_019063.4.
UniGeneiHs.713173.

Genome annotation databases

EnsembliENST00000318522; ENSP00000320663; ENSG00000143924. [Q9HC35-1]
ENST00000402711; ENSP00000385059; ENSG00000143924. [Q9HC35-2]
GeneIDi27436.
KEGGihsa:27436.
UCSCiuc002rsi.3. human. [Q9HC35-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177377 mRNA. Translation: AAG09279.2.
AK001804 mRNA. Translation: BAA91919.1. Different initiation.
AK314702 mRNA. Translation: BAG37250.1.
AC006038 Genomic DNA. Translation: AAY15086.1.
AC083949 Genomic DNA. No translation available.
AC096668 Genomic DNA. Translation: AAY14895.1.
BC023522 mRNA. Translation: AAH23522.1.
BC104647 mRNA. Translation: AAI04648.1. Sequence problems.
BC140845 mRNA. Translation: AAI40846.1.
BC146799 mRNA. Translation: AAI46800.1.
CCDSiCCDS1807.1. [Q9HC35-1]
CCDS46266.1. [Q9HC35-2]
RefSeqiNP_001138548.1. NM_001145076.2.
NP_061936.2. NM_019063.4.
UniGeneiHs.713173.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CGCX-ray2.90A/B/C6-64[»]
ProteinModelPortaliQ9HC35.
SMRiQ9HC35.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118169. 22 interactors.
IntActiQ9HC35. 9 interactors.
MINTiMINT-5005640.
STRINGi9606.ENSP00000320663.

PTM databases

iPTMnetiQ9HC35.
PhosphoSitePlusiQ9HC35.

Polymorphism and mutation databases

BioMutaiEML4.
DMDMi296439364.

Proteomic databases

EPDiQ9HC35.
MaxQBiQ9HC35.
PaxDbiQ9HC35.
PeptideAtlasiQ9HC35.
PRIDEiQ9HC35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318522; ENSP00000320663; ENSG00000143924. [Q9HC35-1]
ENST00000402711; ENSP00000385059; ENSG00000143924. [Q9HC35-2]
GeneIDi27436.
KEGGihsa:27436.
UCSCiuc002rsi.3. human. [Q9HC35-1]

Organism-specific databases

CTDi27436.
DisGeNETi27436.
GeneCardsiEML4.
HGNCiHGNC:1316. EML4.
HPAiHPA036687.
HPA036688.
HPA065337.
MalaCardsiEML4.
MIMi607442. gene.
neXtProtiNX_Q9HC35.
OpenTargetsiENSG00000143924.
Orphaneti357191. Selection of therapeutic option in non-small cell lung carcinoma.
PharmGKBiPA27769.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ISA0. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ9HC35.
KOiK15420.
PhylomeDBiQ9HC35.
TreeFamiTF317832.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143924-MONOMER.
SignaLinkiQ9HC35.

Miscellaneous databases

ChiTaRSiEML4. human.
GeneWikiiEML4.
GenomeRNAii27436.
PROiQ9HC35.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143924.
CleanExiHS_EML4.
ExpressionAtlasiQ9HC35. baseline and differential.
GenevisibleiQ9HC35. HS.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMAL4_HUMAN
AccessioniPrimary (citable) accession number: Q9HC35
Secondary accession number(s): A6H8Y6
, B2RBK3, B2RTW7, B5MCW9, Q3SWW0, Q53R29, Q53TW8, Q6PJ45, Q9NV40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.