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Protein

Echinoderm microtubule-associated protein-like 4

Gene

EML4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.By similarity

GO - Biological processi

  • microtubule-based process Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

SignaLinkiQ9HC35.

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 4
Short name:
EMAP-4
Alternative name(s):
Restrictedly overexpressed proliferation-associated protein
Ropp 120
Gene namesi
Name:EML4
Synonyms:C2orf2, EMAPL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:1316. EML4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

MalaCardsiEML4.
Orphaneti357191. Selection of therapeutic option in non-small cell lung carcinoma.
PharmGKBiPA27769.

Polymorphism and mutation databases

BioMutaiEML4.
DMDMi296439364.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 981981Echinoderm microtubule-associated protein-like 4PRO_0000050963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei7 – 71PhosphoserineCombined sources
Modified residuei13 – 131PhosphoserineCombined sources
Modified residuei16 – 161PhosphoserineCombined sources
Modified residuei61 – 611PhosphoserineCombined sources
Modified residuei96 – 961PhosphothreonineCombined sources
Modified residuei144 – 1441PhosphoserineCombined sources
Modified residuei146 – 1461PhosphoserineCombined sources
Modified residuei171 – 1711PhosphoserineCombined sources
Modified residuei200 – 2001PhosphoserineCombined sources
Modified residuei201 – 2011PhosphothreonineCombined sources
Modified residuei226 – 2261PhosphotyrosineCombined sources
Modified residuei237 – 2371PhosphothreonineCombined sources
Modified residuei891 – 8911PhosphoserineCombined sources
Modified residuei895 – 8951PhosphoserineCombined sources
Modified residuei897 – 8971PhosphothreonineCombined sources
Modified residuei899 – 8991PhosphothreonineCombined sources
Modified residuei903 – 9031PhosphoserineCombined sources
Modified residuei978 – 9781PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9HC35.
MaxQBiQ9HC35.
PaxDbiQ9HC35.
PeptideAtlasiQ9HC35.
PRIDEiQ9HC35.

PTM databases

iPTMnetiQ9HC35.
PhosphoSiteiQ9HC35.

Expressioni

Developmental stagei

Strongly overexpressed during mitosis.

Gene expression databases

BgeeiENSG00000143924.
CleanExiHS_EML4.
ExpressionAtlasiQ9HC35. baseline and differential.
GenevisibleiQ9HC35. HS.

Organism-specific databases

HPAiHPA036687.
HPA036688.
HPA065337.

Interactioni

Protein-protein interaction databases

BioGridi118169. 22 interactions.
IntActiQ9HC35. 7 interactions.
MINTiMINT-5005640.
STRINGi9606.ENSP00000320663.

Structurei

Secondary structure

1
981
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 4326Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CGCX-ray2.90A/B/C6-64[»]
ProteinModelPortaliQ9HC35.
SMRiQ9HC35. Positions 16-44, 216-938.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati307 – 35650WD 1Add
BLAST
Repeati361 – 40444WD 2Add
BLAST
Repeati407 – 44842WD 3Add
BLAST
Repeati454 – 49239WD 4Add
BLAST
Repeati507 – 54640WD 5Add
BLAST
Repeati590 – 62940WD 6Add
BLAST
Repeati673 – 71240WD 7Add
BLAST
Repeati719 – 75840WD 8Add
BLAST
Repeati832 – 87241WD 9Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410ISA0. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ9HC35.
KOiK15420.
PhylomeDBiQ9HC35.
TreeFamiTF317832.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HC35-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGFAGSLDD SISAASTSDV QDRLSALESR VQQQEDEITV LKAALADVLR
60 70 80 90 100
RLAISEDHVA SVKKSVSSKG QPSPRAVIPM SCITNGSGAN RKPSHTSAVS
110 120 130 140 150
IAGKETLSSA AKSGTEKKKE KPQGQREKKE ESHSNDQSPQ IRASPSPQPS
160 170 180 190 200
SQPLQIHRQT PESKNATPTK SIKRPSPAEK SHNSWENSDD SRNKLSKIPS
210 220 230 240 250
TPKLIPKVTK TADKHKDVII NQEGEYIKMF MRGRPITMFI PSDVDNYDDI
260 270 280 290 300
RTELPPEKLK LEWAYGYRGK DCRANVYLLP TGKIVYFIAS VVVLFNYEER
310 320 330 340 350
TQRHYLGHTD CVKCLAIHPD KIRIATGQIA GVDKDGRPLQ PHVRVWDSVT
360 370 380 390 400
LSTLQIIGLG TFERGVGCLD FSKADSGVHL CIIDDSNEHM LTVWDWQKKA
410 420 430 440 450
KGAEIKTTNE VVLAVEFHPT DANTIITCGK SHIFFWTWSG NSLTRKQGIF
460 470 480 490 500
GKYEKPKFVQ CLAFLGNGDV LTGDSGGVML IWSKTTVEPT PGKGPKGVYQ
510 520 530 540 550
ISKQIKAHDG SVFTLCQMRN GMLLTGGGKD RKIILWDHDL NPEREIEVPD
560 570 580 590 600
QYGTIRAVAE GKADQFLVGT SRNFILRGTF NDGFQIEVQG HTDELWGLAT
610 620 630 640 650
HPFKDLLLTC AQDRQVCLWN SMEHRLEWTR LVDEPGHCAD FHPSGTVVAI
660 670 680 690 700
GTHSGRWFVL DAETRDLVSI HTDGNEQLSV MRYSIDGTFL AVGSHDNFIY
710 720 730 740 750
LYVVSENGRK YSRYGRCTGH SSYITHLDWS PDNKYIMSNS GDYEILYWDI
760 770 780 790 800
PNGCKLIRNR SDCKDIDWTT YTCVLGFQVF GVWPEGSDGT DINALVRSHN
810 820 830 840 850
RKVIAVADDF CKVHLFQYPC SKAKAPSHKY SAHSSHVTNV SFTHNDSHLI
860 870 880 890 900
STGGKDMSII QWKLVEKLSL PQNETVADTT LTKAPVSSTE SVIQSNTPTP
910 920 930 940 950
PPSQPLNETA EEESRISSSP TLLENSLEQT VEPSEDHSEE ESEEGSGDLG
960 970 980
EPLYEEPCNE ISKEQAKATL LEDQQDPSPS S
Length:981
Mass (Da):108,916
Last modified:May 18, 2010 - v3
Checksum:i7E8F2585747736B9
GO
Isoform 2 (identifier: Q9HC35-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     112-169: Missing.

Note: No experimental confirmation available.
Show »
Length:923
Mass (Da):102,444
Checksum:i82F2C3F43C4C8836
GO

Sequence cautioni

The sequence AAI04648 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA91919 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti768 – 7736WTTYTC → SSRPCW in AAY15086 (PubMed:15815621).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti283 – 2831K → E.3 Publications
Corresponds to variant rs6736913 [ dbSNP | Ensembl ].
VAR_031726
Natural varianti382 – 3821I → V.3 Publications
Corresponds to variant rs10202624 [ dbSNP | Ensembl ].
VAR_031727
Natural varianti398 – 3981K → R.2 Publications
Corresponds to variant rs28651764 [ dbSNP | Ensembl ].
VAR_031728
Natural varianti978 – 9781S → L.
Corresponds to variant rs28364731 [ dbSNP | Ensembl ].
VAR_031729

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei112 – 16958Missing in isoform 2. 1 PublicationVSP_047192Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177377 mRNA. Translation: AAG09279.2.
AK001804 mRNA. Translation: BAA91919.1. Different initiation.
AK314702 mRNA. Translation: BAG37250.1.
AC006038 Genomic DNA. Translation: AAY15086.1.
AC083949 Genomic DNA. No translation available.
AC096668 Genomic DNA. Translation: AAY14895.1.
BC023522 mRNA. Translation: AAH23522.1.
BC104647 mRNA. Translation: AAI04648.1. Sequence problems.
BC140845 mRNA. Translation: AAI40846.1.
BC146799 mRNA. Translation: AAI46800.1.
CCDSiCCDS1807.1. [Q9HC35-1]
CCDS46266.1. [Q9HC35-2]
RefSeqiNP_001138548.1. NM_001145076.2.
NP_061936.2. NM_019063.4.
UniGeneiHs.713173.

Genome annotation databases

EnsembliENST00000318522; ENSP00000320663; ENSG00000143924. [Q9HC35-1]
ENST00000402711; ENSP00000385059; ENSG00000143924. [Q9HC35-2]
GeneIDi27436.
KEGGihsa:27436.
UCSCiuc002rsi.3. human. [Q9HC35-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177377 mRNA. Translation: AAG09279.2.
AK001804 mRNA. Translation: BAA91919.1. Different initiation.
AK314702 mRNA. Translation: BAG37250.1.
AC006038 Genomic DNA. Translation: AAY15086.1.
AC083949 Genomic DNA. No translation available.
AC096668 Genomic DNA. Translation: AAY14895.1.
BC023522 mRNA. Translation: AAH23522.1.
BC104647 mRNA. Translation: AAI04648.1. Sequence problems.
BC140845 mRNA. Translation: AAI40846.1.
BC146799 mRNA. Translation: AAI46800.1.
CCDSiCCDS1807.1. [Q9HC35-1]
CCDS46266.1. [Q9HC35-2]
RefSeqiNP_001138548.1. NM_001145076.2.
NP_061936.2. NM_019063.4.
UniGeneiHs.713173.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CGCX-ray2.90A/B/C6-64[»]
ProteinModelPortaliQ9HC35.
SMRiQ9HC35. Positions 16-44, 216-938.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118169. 22 interactions.
IntActiQ9HC35. 7 interactions.
MINTiMINT-5005640.
STRINGi9606.ENSP00000320663.

PTM databases

iPTMnetiQ9HC35.
PhosphoSiteiQ9HC35.

Polymorphism and mutation databases

BioMutaiEML4.
DMDMi296439364.

Proteomic databases

EPDiQ9HC35.
MaxQBiQ9HC35.
PaxDbiQ9HC35.
PeptideAtlasiQ9HC35.
PRIDEiQ9HC35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318522; ENSP00000320663; ENSG00000143924. [Q9HC35-1]
ENST00000402711; ENSP00000385059; ENSG00000143924. [Q9HC35-2]
GeneIDi27436.
KEGGihsa:27436.
UCSCiuc002rsi.3. human. [Q9HC35-1]

Organism-specific databases

CTDi27436.
GeneCardsiEML4.
HGNCiHGNC:1316. EML4.
HPAiHPA036687.
HPA036688.
HPA065337.
MalaCardsiEML4.
MIMi607442. gene.
neXtProtiNX_Q9HC35.
Orphaneti357191. Selection of therapeutic option in non-small cell lung carcinoma.
PharmGKBiPA27769.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ISA0. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00550000074369.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ9HC35.
KOiK15420.
PhylomeDBiQ9HC35.
TreeFamiTF317832.

Enzyme and pathway databases

SignaLinkiQ9HC35.

Miscellaneous databases

ChiTaRSiEML4. human.
GeneWikiiEML4.
GenomeRNAii27436.
PROiQ9HC35.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143924.
CleanExiHS_EML4.
ExpressionAtlasiQ9HC35. baseline and differential.
GenevisibleiQ9HC35. HS.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMAL4_HUMAN
AccessioniPrimary (citable) accession number: Q9HC35
Secondary accession number(s): A6H8Y6
, B2RBK3, B2RTW7, B5MCW9, Q3SWW0, Q53R29, Q53TW8, Q6PJ45, Q9NV40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.