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Q9HC24 (LFG4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein lifeguard 4
Alternative name(s):
Golgi anti-apoptotic protein
Protein S1R
Transmembrane BAX inhibitor motif-containing protein 4
Z-protein
Gene names
Name:TMBIM4
Synonyms:GAAP, LFG4
ORF Names:CGI-119
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Anti-apoptotic protein which can inhibit apoptosis induced by intrinsic and extrinsic apoptotic stimuli. Can modulate both capacitative Ca2+ entry and inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release. Ref.7 Ref.9

Subunit structure

Interacts with ITPR3. Ref.9

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein Ref.7 Ref.10.

Sequence similarities

Belongs to the BI1 family. LFG subfamily.

Sequence caution

The sequence AAD34114.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Protein lifeguard 4
PRO_0000179092

Regions

Topological domain1 – 3838Cytoplasmic Potential
Transmembrane39 – 5921Helical; Potential
Topological domain60 – 689Lumenal Potential
Transmembrane69 – 8921Helical; Potential
Topological domain90 – 978Cytoplasmic Potential
Transmembrane98 – 11821Helical; Potential
Topological domain119 – 1202Lumenal Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 15110Cytoplasmic Potential
Transmembrane152 – 17221Helical; Potential
Topological domain173 – 1753Lumenal Potential
Transmembrane176 – 19621Helical; Potential
Topological domain197 – 20812Cytoplasmic Potential
Intramembrane209 – 22921Helical; Potential
Topological domain230 – 2389Cytoplasmic Potential

Natural variations

Natural variant21A → V.
Corresponds to variant rs11176070 [ dbSNP | Ensembl ].
VAR_055547
Natural variant881I → T. Ref.2 Ref.3 Ref.4
Corresponds to variant rs8793 [ dbSNP | Ensembl ].
VAR_014946

Sequences

Sequence LengthMass (Da)Tools
Q9HC24 [UniParc].

Last modified February 8, 2011. Version 3.
Checksum: 30CD39CF7F38564E

FASTA23826,971
        10         20         30         40         50         60 
MADPDPRYPR SSIEDDFNYG SSVASATVHI RMAFLRKVYS ILSLQVLLTT VTSTVFLYFE 

        70         80         90        100        110        120 
SVRTFVHESP ALILLFALGS LGLIFALILN RHKYPLNLYL LFGFTLLEAL TVAVVVTFYD 

       130        140        150        160        170        180 
VYIILQAFIL TTTVFFGLTV YTLQSKKDFS KFGAGLFALL WILCLSGFLK FFFYSEIMEL 

       190        200        210        220        230 
VLAAAGALLF CGFIIYDTHS LMHKLSPEEY VLAAISLYLD IINLFLHLLR FLEAVNKK 

« Hide

References

« Hide 'large scale' references
[1]"Identification and phylogeny of a novel family of eukaryotic membrane proteins related to a glutamate binding protein (GBP)."
Goldberger O., Smith T., Minta J., Goldberger G.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88.
[3]"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. expand/collapse author list , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88.
Tissue: Adrenal gland.
[4]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88.
Tissue: Placenta.
[5]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"A new inhibitor of apoptosis from vaccinia virus and eukaryotes."
Gubser C., Bergamaschi D., Hollinshead M., Lu X., van Kuppeveld F.J., Smith G.L.
PLoS Pathog. 3:E17-E17(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"LFG: a candidate apoptosis regulatory gene family."
Hu L., Smith T.F., Goldberger G.
Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"Human Golgi antiapoptotic protein modulates intracellular calcium fluxes."
de Mattia F., Gubser C., van Dommelen M.M., Visch H.J., Distelmaier F., Postigo A., Luyten T., Parys J.B., de Smedt H., Smith G.L., Willems P.H., van Kuppeveld F.J.
Mol. Biol. Cell 20:3638-3645(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ITPR3.
[10]"Six-transmembrane topology for Golgi anti-apoptotic protein (GAAP) and Bax inhibitor 1 (BI-1) provides model for the transmembrane Bax inhibitor-containing motif (TMBIM) family."
Carrara G., Saraiva N., Gubser C., Johnson B.F., Smith G.L.
J. Biol. Chem. 287:15896-15905(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF182041 mRNA. Translation: AAG16898.1.
AF151877 mRNA. Translation: AAD34114.1. Different initiation.
AF113127 mRNA. Translation: AAF14868.1.
AK075069 mRNA. Translation: BAC11384.1.
AC078927 Genomic DNA. No translation available.
BC020613 mRNA. No translation available.
RefSeqNP_001269535.1. NM_001282606.1.
NP_001269538.1. NM_001282609.1.
NP_001269539.1. NM_001282610.1.
NP_057140.2. NM_016056.3.
UniGeneHs.505934.

3D structure databases

ProteinModelPortalQ9HC24.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119653. 10 interactions.
IntActQ9HC24. 4 interactions.
STRING9606.ENSP00000350965.

Protein family/group databases

TCDB1.A.14.3.6. the testis-enhanced gene transfer (tegt) family.

Polymorphism databases

DMDM322510100.

Proteomic databases

PaxDbQ9HC24.

Protocols and materials databases

DNASU51643.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000358230; ENSP00000350965; ENSG00000155957.
GeneID51643.
KEGGhsa:51643.
UCSCuc001stc.3. human.

Organism-specific databases

CTD51643.
GeneCardsGC12M066531.
HGNCHGNC:24257. TMBIM4.
neXtProtNX_Q9HC24.
PharmGKBPA142670805.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0670.
HOGENOMHOG000060393.
HOVERGENHBG012084.
KOK06890.
OrthoDBEOG7MPRFW.
PhylomeDBQ9HC24.
TreeFamTF321860.

Gene expression databases

ArrayExpressQ9HC24.
BgeeQ9HC24.
CleanExHS_TMBIM4.
GenevestigatorQ9HC24.

Family and domain databases

InterProIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERPTHR23291. PTHR23291. 1 hit.
PfamPF01027. Bax1-I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTMBIM4.
GenomeRNAi51643.
NextBio55584.
PROQ9HC24.

Entry information

Entry nameLFG4_HUMAN
AccessionPrimary (citable) accession number: Q9HC24
Secondary accession number(s): Q542Z6, Q9UHY5, Q9Y3C2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: February 8, 2011
Last modified: April 16, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM