Q9HC24 (LFG4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein lifeguard 4

Alternative name(s):
Golgi anti-apoptotic protein
Protein S1R
Transmembrane BAX inhibitor motif-containing protein 4
Z-protein

Gene names

Name:	TMBIM4
Synonyms:	GAAP, LFG4
ORF Names:	CGI-119

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Anti-apoptotic protein which can inhibit apoptosis induced by intrinsic and extrinsic apoptotic stimuli. Can modulate both capacitative Ca2+ entry and inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release. Ref.7 Ref.9
Subunit structure	Interacts with ITPR3. Ref.9
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein Ref.7 Ref.10.
Sequence similarities	Belongs to the BI1 family. LFG subfamily.
Sequence caution	The sequence AAD34114.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Inferred from mutant phenotype Ref.7. Source: UniProtKB regulation of calcium-mediated signaling Inferred from direct assay Ref.9. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from direct assay Ref.7. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Protein lifeguard 4		PRO_0000179092
Regions
	Topological domain	1 – 38	38	Cytoplasmic Potential
	Transmembrane	39 – 59	21	Helical; Potential
	Topological domain	60 – 68	9	Lumenal Potential
	Transmembrane	69 – 89	21	Helical; Potential
	Topological domain	90 – 97	8	Cytoplasmic Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Topological domain	119 – 120	2	Lumenal Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Topological domain	142 – 151	10	Cytoplasmic Potential
	Transmembrane	152 – 172	21	Helical; Potential
	Topological domain	173 – 175	3	Lumenal Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Topological domain	197 – 208	12	Cytoplasmic Potential
	Intramembrane	209 – 229	21	Helical; Potential
	Topological domain	230 – 238	9	Cytoplasmic Potential
Natural variations
	Natural variant	2	1	A → V. Corresponds to variant rs11176070 [ dbSNP | Ensembl ].		VAR_055547
	Natural variant	88	1	I → T. Ref.2 Ref.3 Ref.4 Corresponds to variant rs8793 [ dbSNP | Ensembl ].		VAR_014946

Sequences

	Sequence		Length	Mass (Da)
	Q9HC24 [UniParc]. Last modified February 8, 2011. Version 3. Checksum: 30CD39CF7F38564E	FASTA	238	26,971
	10 20 30 40 50 60 MADPDPRYPR SSIEDDFNYG SSVASATVHI RMAFLRKVYS ILSLQVLLTT VTSTVFLYFE 70 80 90 100 110 120 SVRTFVHESP ALILLFALGS LGLIFALILN RHKYPLNLYL LFGFTLLEAL TVAVVVTFYD 130 140 150 160 170 180 VYIILQAFIL TTTVFFGLTV YTLQSKKDFS KFGAGLFALL WILCLSGFLK FFFYSEIMEL 190 200 210 220 230 VLAAAGALLF CGFIIYDTHS LMHKLSPEEY VLAAISLYLD IINLFLHLLR FLEAVNKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and phylogeny of a novel family of eukaryotic membrane proteins related to a glutamate binding protein (GBP)." Goldberger O., Smith T., Minta J., Goldberger G. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88.
[3]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88. Tissue: Adrenal gland.
[4]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88. Tissue: Placenta.
[5]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"A new inhibitor of apoptosis from vaccinia virus and eukaryotes." Gubser C., Bergamaschi D., Hollinshead M., Lu X., van Kuppeveld F.J., Smith G.L. PLoS Pathog. 3:E17-E17(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]	"LFG: a candidate apoptosis regulatory gene family." Hu L., Smith T.F., Goldberger G. Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[9]	"Human Golgi antiapoptotic protein modulates intracellular calcium fluxes." de Mattia F., Gubser C., van Dommelen M.M., Visch H.J., Distelmaier F., Postigo A., Luyten T., Parys J.B., de Smedt H., Smith G.L., Willems P.H., van Kuppeveld F.J. Mol. Biol. Cell 20:3638-3645(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ITPR3.
[10]	"Six-transmembrane topology for Golgi anti-apoptotic protein (GAAP) and Bax inhibitor 1 (BI-1) provides model for the transmembrane Bax inhibitor-containing motif (TMBIM) family." Carrara G., Saraiva N., Gubser C., Johnson B.F., Smith G.L. J. Biol. Chem. 287:15896-15905(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF182041 mRNA. Translation: AAG16898.1. AF151877 mRNA. Translation: AAD34114.1. Different initiation. AF113127 mRNA. Translation: AAF14868.1. AK075069 mRNA. Translation: BAC11384.1. AC078927 Genomic DNA. No translation available. BC020613 mRNA. No translation available.
IPI	IPI00219780.
RefSeq	NP_057140.2. NM_016056.2.
UniGene	Hs.505934.
3D structure databases
ProteinModelPortal	Q9HC24.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HC24. 3 interactions.
STRING	9606.ENSP00000350965.
Polymorphism databases
DMDM	15214407.
Proteomic databases
PaxDb	Q9HC24.
Protocols and materials databases
DNASU	51643.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000358230; ENSP00000350965; ENSG00000155957.
GeneID	51643.
KEGG	hsa:51643.
UCSC	uc001stc.3. human.
Organism-specific databases
CTD	51643.
GeneCards	GC12M066531.
HGNC	HGNC:24257. TMBIM4.
neXtProt	NX_Q9HC24.
PharmGKB	PA142670805.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0670.
HOGENOM	HOG000060393.
HOVERGEN	HBG012084.
KO	K06890.
Gene expression databases
ArrayExpress	Q9HC24.
Bgee	Q9HC24.
CleanEx	HS_TMBIM4.
Genevestigator	Q9HC24.
GermOnline	ENSG00000155957. Homo sapiens.
Family and domain databases
InterPro	IPR006214. Bax_inhibitor_1-related. [Graphical view]
PANTHER	PTHR23291. PTHR23291. 1 hit.
Pfam	PF01027. Bax1-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51643.
NextBio	55584.

Entry information

Entry name

LFG4_HUMAN

Accession

Primary (citable) accession number: Q9HC24
Secondary accession number(s): Q542Z6, Q9UHY5, Q9Y3C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	February 8, 2011
Last modified:	April 3, 2013
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.