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Protein

Protein lifeguard 4

Gene

TMBIM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Anti-apoptotic protein which can inhibit apoptosis induced by intrinsic and extrinsic apoptotic stimuli. Can modulate both capacitative Ca2+ entry and inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release.2 Publications

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. negative regulation of apoptotic process Source: UniProtKB
  3. regulation of calcium-mediated signaling Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Protein family/group databases

TCDBi1.A.14.3.6. the testis-enhanced gene transfer (tegt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lifeguard 4
Alternative name(s):
Golgi anti-apoptotic protein
Protein S1R
Transmembrane BAX inhibitor motif-containing protein 4
Z-protein
Gene namesi
Name:TMBIM4
Synonyms:GAAP, LFG4
ORF Names:CGI-119
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:24257. TMBIM4.

Subcellular locationi

Golgi apparatus membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3838CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei39 – 5921HelicalSequence AnalysisAdd
BLAST
Topological domaini60 – 689LumenalSequence Analysis
Transmembranei69 – 8921HelicalSequence AnalysisAdd
BLAST
Topological domaini90 – 978CytoplasmicSequence Analysis
Transmembranei98 – 11821HelicalSequence AnalysisAdd
BLAST
Topological domaini119 – 1202LumenalSequence Analysis
Transmembranei121 – 14121HelicalSequence AnalysisAdd
BLAST
Topological domaini142 – 15110CytoplasmicSequence Analysis
Transmembranei152 – 17221HelicalSequence AnalysisAdd
BLAST
Topological domaini173 – 1753LumenalSequence Analysis
Transmembranei176 – 19621HelicalSequence AnalysisAdd
BLAST
Topological domaini197 – 20812CytoplasmicSequence AnalysisAdd
BLAST
Intramembranei209 – 22921HelicalSequence AnalysisAdd
BLAST
Topological domaini230 – 2389CytoplasmicSequence Analysis

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. Golgi stack Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670805.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Protein lifeguard 4PRO_0000179092Add
BLAST

Proteomic databases

PaxDbiQ9HC24.

Expressioni

Gene expression databases

BgeeiQ9HC24.
CleanExiHS_TMBIM4.
ExpressionAtlasiQ9HC24. baseline and differential.
GenevestigatoriQ9HC24.

Interactioni

Subunit structurei

Interacts with ITPR3.1 Publication

Protein-protein interaction databases

BioGridi119653. 10 interactions.
IntActiQ9HC24. 4 interactions.
STRINGi9606.ENSP00000350965.

Structurei

3D structure databases

ProteinModelPortaliQ9HC24.
SMRiQ9HC24. Positions 71-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BI1 family. LFG subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0670.
GeneTreeiENSGT00500000044791.
HOGENOMiHOG000060393.
HOVERGENiHBG012084.
InParanoidiQ9HC24.
KOiK06890.
OrthoDBiEOG7MPRFW.
PhylomeDBiQ9HC24.
TreeFamiTF321860.

Family and domain databases

InterProiIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERiPTHR23291. PTHR23291. 1 hit.
PfamiPF01027. Bax1-I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HC24-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADPDPRYPR SSIEDDFNYG SSVASATVHI RMAFLRKVYS ILSLQVLLTT
60 70 80 90 100
VTSTVFLYFE SVRTFVHESP ALILLFALGS LGLIFALILN RHKYPLNLYL
110 120 130 140 150
LFGFTLLEAL TVAVVVTFYD VYIILQAFIL TTTVFFGLTV YTLQSKKDFS
160 170 180 190 200
KFGAGLFALL WILCLSGFLK FFFYSEIMEL VLAAAGALLF CGFIIYDTHS
210 220 230
LMHKLSPEEY VLAAISLYLD IINLFLHLLR FLEAVNKK
Length:238
Mass (Da):26,971
Last modified:February 8, 2011 - v3
Checksum:i30CD39CF7F38564E
GO

Sequence cautioni

The sequence AAD34114.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2 – 21A → V.
Corresponds to variant rs11176070 [ dbSNP | Ensembl ].
VAR_055547
Natural varianti88 – 881I → T.3 Publications
Corresponds to variant rs8793 [ dbSNP | Ensembl ].
VAR_014946

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182041 mRNA. Translation: AAG16898.1.
AF151877 mRNA. Translation: AAD34114.1. Different initiation.
AF113127 mRNA. Translation: AAF14868.1.
AK075069 mRNA. Translation: BAC11384.1.
AC078927 Genomic DNA. No translation available.
BC020613 mRNA. No translation available.
CCDSiCCDS41805.1.
RefSeqiNP_001269535.1. NM_001282606.1.
NP_001269538.1. NM_001282609.1.
NP_001269539.1. NM_001282610.1.
NP_057140.2. NM_016056.3.
UniGeneiHs.505934.

Genome annotation databases

EnsembliENST00000358230; ENSP00000350965; ENSG00000155957.
GeneIDi51643.
KEGGihsa:51643.
UCSCiuc001stc.3. human.

Polymorphism databases

DMDMi322510100.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182041 mRNA. Translation: AAG16898.1.
AF151877 mRNA. Translation: AAD34114.1. Different initiation.
AF113127 mRNA. Translation: AAF14868.1.
AK075069 mRNA. Translation: BAC11384.1.
AC078927 Genomic DNA. No translation available.
BC020613 mRNA. No translation available.
CCDSiCCDS41805.1.
RefSeqiNP_001269535.1. NM_001282606.1.
NP_001269538.1. NM_001282609.1.
NP_001269539.1. NM_001282610.1.
NP_057140.2. NM_016056.3.
UniGeneiHs.505934.

3D structure databases

ProteinModelPortaliQ9HC24.
SMRiQ9HC24. Positions 71-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119653. 10 interactions.
IntActiQ9HC24. 4 interactions.
STRINGi9606.ENSP00000350965.

Protein family/group databases

TCDBi1.A.14.3.6. the testis-enhanced gene transfer (tegt) family.

Polymorphism databases

DMDMi322510100.

Proteomic databases

PaxDbiQ9HC24.

Protocols and materials databases

DNASUi51643.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358230; ENSP00000350965; ENSG00000155957.
GeneIDi51643.
KEGGihsa:51643.
UCSCiuc001stc.3. human.

Organism-specific databases

CTDi51643.
GeneCardsiGC12M066518.
HGNCiHGNC:24257. TMBIM4.
neXtProtiNX_Q9HC24.
PharmGKBiPA142670805.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0670.
GeneTreeiENSGT00500000044791.
HOGENOMiHOG000060393.
HOVERGENiHBG012084.
InParanoidiQ9HC24.
KOiK06890.
OrthoDBiEOG7MPRFW.
PhylomeDBiQ9HC24.
TreeFamiTF321860.

Miscellaneous databases

GeneWikiiTMBIM4.
GenomeRNAii51643.
NextBioi55584.
PROiQ9HC24.

Gene expression databases

BgeeiQ9HC24.
CleanExiHS_TMBIM4.
ExpressionAtlasiQ9HC24. baseline and differential.
GenevestigatoriQ9HC24.

Family and domain databases

InterProiIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERiPTHR23291. PTHR23291. 1 hit.
PfamiPF01027. Bax1-I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and phylogeny of a novel family of eukaryotic membrane proteins related to a glutamate binding protein (GBP)."
    Goldberger O., Smith T., Minta J., Goldberger G.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
    Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.
    Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88.
    Tissue: Adrenal gland.
  4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-88.
    Tissue: Placenta.
  5. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "A new inhibitor of apoptosis from vaccinia virus and eukaryotes."
    Gubser C., Bergamaschi D., Hollinshead M., Lu X., van Kuppeveld F.J., Smith G.L.
    PLoS Pathog. 3:E17-E17(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "LFG: a candidate apoptosis regulatory gene family."
    Hu L., Smith T.F., Goldberger G.
    Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. Cited for: FUNCTION, INTERACTION WITH ITPR3.
  10. "Six-transmembrane topology for Golgi anti-apoptotic protein (GAAP) and Bax inhibitor 1 (BI-1) provides model for the transmembrane Bax inhibitor-containing motif (TMBIM) family."
    Carrara G., Saraiva N., Gubser C., Johnson B.F., Smith G.L.
    J. Biol. Chem. 287:15896-15905(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.

Entry informationi

Entry nameiLFG4_HUMAN
AccessioniPrimary (citable) accession number: Q9HC24
Secondary accession number(s): Q542Z6, Q9UHY5, Q9Y3C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: February 8, 2011
Last modified: January 7, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.