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Protein

Serine/threonine-protein kinase Sgk2

Gene

SGK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na+ channels: SCNN1A/ENAC, K+ channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Two specific sites, one in the kinase domain (Thr-253) and the other in the C-terminal regulatory region (Ser-416), need to be phosphorylated for its full activation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124ATPPROSITE-ProRule annotation1
Active sitei219Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi101 – 109ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium channel regulator activity Source: GO_Central
  • chloride channel regulator activity Source: GO_Central
  • potassium channel regulator activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: ProtInc
  • sodium channel regulator activity Source: UniProtKB

GO - Biological processi

  • cellular sodium ion homeostasis Source: GO_Central
  • intracellular signal transduction Source: ProtInc
  • ion transmembrane transport Source: Reactome
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of sodium ion transport Source: GO_Central
  • positive regulation of transporter activity Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • regulation of apoptotic process Source: GO_Central
  • regulation of cell growth Source: UniProtKB
  • regulation of cell proliferation Source: UniProtKB
  • response to oxidative stress Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS02185-MONOMER.
ReactomeiR-HSA-2672351. Stimuli-sensing channels.
SignaLinkiQ9HBY8.
SIGNORiQ9HBY8.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Sgk2 (EC:2.7.11.1)
Alternative name(s):
Serum/glucocorticoid-regulated kinase 2
Gene namesi
Name:SGK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:13900. SGK2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi416S → D: Increased activation. 1 Publication1

Organism-specific databases

DisGeNETi10110.
OpenTargetsiENSG00000101049.
PharmGKBiPA37824.

Chemistry databases

ChEMBLiCHEMBL5794.
GuidetoPHARMACOLOGYi1535.

Polymorphism and mutation databases

BioMutaiSGK2.
DMDMi28558166.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866461 – 427Serine/threonine-protein kinase Sgk2Add BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70PhosphoserineBy similarity1
Modified residuei253Phosphothreonine; by PDPK11 Publication1
Modified residuei416PhosphoserineSequence analysis1

Post-translational modificationi

Activated by phosphorylation on Ser-416 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-253.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9HBY8.
PaxDbiQ9HBY8.
PeptideAtlasiQ9HBY8.
PRIDEiQ9HBY8.

PTM databases

iPTMnetiQ9HBY8.
PhosphoSitePlusiQ9HBY8.

Expressioni

Tissue specificityi

Highly expressed in liver, kidney and pancreas, and at lower levels in brain.1 Publication

Gene expression databases

BgeeiENSG00000101049.
CleanExiHS_SGK2.
ExpressionAtlasiQ9HBY8. baseline and differential.
GenevisibleiQ9HBY8. HS.

Organism-specific databases

HPAiHPA011387.

Interactioni

Protein-protein interaction databases

BioGridi115416. 16 interactors.
IntActiQ9HBY8. 14 interactors.
STRINGi9606.ENSP00000340608.

Chemistry databases

BindingDBiQ9HBY8.

Structurei

3D structure databases

ProteinModelPortaliQ9HBY8.
SMRiQ9HBY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 352Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini353 – 427AGC-kinase C-terminalAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi128 – 138Nuclear localization signalBy similarityAdd BLAST11

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00860000133668.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ9HBY8.
KOiK13303.
OrthoDBiEOG091G06FF.
PhylomeDBiQ9HBY8.
TreeFamiTF320906.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q9HBY8-1) [UniParc]FASTAAdd to basket
Also known as: beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGLLTSGRK PSGGGRCTGR GGWRGQWCLK PWMGGADPPT PTLSCLLLPV
60 70 80 90 100
PPELPDHCYR MNSSPAGTPS PQPSRANGNI NLGPSANPNA QPTDFDFLKV
110 120 130 140 150
IGKGNYGKVL LAKRKSDGAF YAVKVLQKKS ILKKKEQSHI MAERSVLLKN
160 170 180 190 200
VRHPFLVGLR YSFQTPEKLY FVLDYVNGGE LFFHLQRERR FLEPRARFYA
210 220 230 240 250
AEVASAIGYL HSLNIIYRDL KPENILLDCQ GHVVLTDFGL CKEGVEPEDT
260 270 280 290 300
TSTFCGTPEY LAPEVLRKEP YDRAVDWWCL GAVLYEMLHG LPPFYSQDVS
310 320 330 340 350
QMYENILHQP LQIPGGRTVA ACDLLQSLLH KDQRQRLGSK ADFLEIKNHV
360 370 380 390 400
FFSPINWDDL YHKRLTPPFN PNVTGPADLK HFDPEFTQEA VSKSIGCTPD
410 420
TVASSSGASS AFLGFSYAPE DDDILDC
Length:427
Mass (Da):47,604
Last modified:March 1, 2001 - v1
Checksum:iD8F0FA6DF54B1370
GO
Isoform 1 (identifier: Q9HBY8-2) [UniParc]FASTAAdd to basket
Also known as: alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Show »
Length:367
Mass (Da):41,175
Checksum:iE53F1ABE253F648B
GO
Isoform 3 (identifier: Q9HBY8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     19-52: Missing.

Note: No experimental confirmation available.
Show »
Length:393
Mass (Da):43,977
Checksum:iDB7303AF95D844CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137Missing in CAI42315 (PubMed:11780052).Curated1
Sequence conflicti168K → E in AAX88805 (Ref. 2) Curated1
Sequence conflicti208G → D in AAV38901 (Ref. 3) Curated1
Sequence conflicti337L → P in AAX88805 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04107312S → T.1 PublicationCorresponds to variant rs33969356dbSNPEnsembl.1
Natural variantiVAR_041074259E → K in a lung adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041075349H → Y.1 PublicationCorresponds to variant rs35793869dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0049321 – 60Missing in isoform 1. 3 PublicationsAdd BLAST60
Alternative sequenceiVSP_02668219 – 52Missing in isoform 3. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169034 mRNA. Translation: AAF12757.2.
AF186470 mRNA. Translation: AAG17012.1.
AY987010 mRNA. Translation: AAX88805.1.
BT020098 mRNA. Translation: AAV38901.1.
Z98752 Genomic DNA. Translation: CAC18509.1.
Z98752 Genomic DNA. Translation: CAI42312.1.
Z98752 Genomic DNA. Translation: CAI42315.1.
BC014037 mRNA. Translation: AAH14037.3.
BC065511 mRNA. Translation: AAH65511.1.
CCDSiCCDS13320.1. [Q9HBY8-1]
CCDS13321.1. [Q9HBY8-2]
RefSeqiNP_001186193.1. NM_001199264.1. [Q9HBY8-2]
NP_057360.2. NM_016276.3. [Q9HBY8-1]
NP_733794.1. NM_170693.2. [Q9HBY8-2]
UniGeneiHs.300863.
Hs.740147.

Genome annotation databases

EnsembliENST00000341458; ENSP00000340608; ENSG00000101049. [Q9HBY8-1]
ENST00000373092; ENSP00000362184; ENSG00000101049. [Q9HBY8-2]
ENST00000373100; ENSP00000362192; ENSG00000101049. [Q9HBY8-2]
ENST00000423407; ENSP00000392795; ENSG00000101049. [Q9HBY8-2]
ENST00000426287; ENSP00000412214; ENSG00000101049. [Q9HBY8-3]
GeneIDi10110.
KEGGihsa:10110.
UCSCiuc002xkr.4. human. [Q9HBY8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF169034 mRNA. Translation: AAF12757.2.
AF186470 mRNA. Translation: AAG17012.1.
AY987010 mRNA. Translation: AAX88805.1.
BT020098 mRNA. Translation: AAV38901.1.
Z98752 Genomic DNA. Translation: CAC18509.1.
Z98752 Genomic DNA. Translation: CAI42312.1.
Z98752 Genomic DNA. Translation: CAI42315.1.
BC014037 mRNA. Translation: AAH14037.3.
BC065511 mRNA. Translation: AAH65511.1.
CCDSiCCDS13320.1. [Q9HBY8-1]
CCDS13321.1. [Q9HBY8-2]
RefSeqiNP_001186193.1. NM_001199264.1. [Q9HBY8-2]
NP_057360.2. NM_016276.3. [Q9HBY8-1]
NP_733794.1. NM_170693.2. [Q9HBY8-2]
UniGeneiHs.300863.
Hs.740147.

3D structure databases

ProteinModelPortaliQ9HBY8.
SMRiQ9HBY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115416. 16 interactors.
IntActiQ9HBY8. 14 interactors.
STRINGi9606.ENSP00000340608.

Chemistry databases

BindingDBiQ9HBY8.
ChEMBLiCHEMBL5794.
GuidetoPHARMACOLOGYi1535.

PTM databases

iPTMnetiQ9HBY8.
PhosphoSitePlusiQ9HBY8.

Polymorphism and mutation databases

BioMutaiSGK2.
DMDMi28558166.

Proteomic databases

MaxQBiQ9HBY8.
PaxDbiQ9HBY8.
PeptideAtlasiQ9HBY8.
PRIDEiQ9HBY8.

Protocols and materials databases

DNASUi10110.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341458; ENSP00000340608; ENSG00000101049. [Q9HBY8-1]
ENST00000373092; ENSP00000362184; ENSG00000101049. [Q9HBY8-2]
ENST00000373100; ENSP00000362192; ENSG00000101049. [Q9HBY8-2]
ENST00000423407; ENSP00000392795; ENSG00000101049. [Q9HBY8-2]
ENST00000426287; ENSP00000412214; ENSG00000101049. [Q9HBY8-3]
GeneIDi10110.
KEGGihsa:10110.
UCSCiuc002xkr.4. human. [Q9HBY8-1]

Organism-specific databases

CTDi10110.
DisGeNETi10110.
GeneCardsiSGK2.
H-InvDBHIX0080189.
HGNCiHGNC:13900. SGK2.
HPAiHPA011387.
MIMi607589. gene.
neXtProtiNX_Q9HBY8.
OpenTargetsiENSG00000101049.
PharmGKBiPA37824.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00860000133668.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ9HBY8.
KOiK13303.
OrthoDBiEOG091G06FF.
PhylomeDBiQ9HBY8.
TreeFamiTF320906.

Enzyme and pathway databases

BioCyciZFISH:HS02185-MONOMER.
ReactomeiR-HSA-2672351. Stimuli-sensing channels.
SignaLinkiQ9HBY8.
SIGNORiQ9HBY8.

Miscellaneous databases

GeneWikiiSGK2.
GenomeRNAii10110.
PROiQ9HBY8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000101049.
CleanExiHS_SGK2.
ExpressionAtlasiQ9HBY8. baseline and differential.
GenevisibleiQ9HBY8. HS.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSGK2_HUMAN
AccessioniPrimary (citable) accession number: Q9HBY8
Secondary accession number(s): Q52PK5
, Q5H8Y6, Q5H8Z1, Q5TZR3, Q9UKG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Not regulated by serum or glucocorticoids.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.