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Q9HBW1 (LRRC4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine-rich repeat-containing protein 4
Alternative name(s):
Brain tumor-associated protein BAG
Nasopharyngeal carcinoma-associated gene 14 protein
Netrin-G2 ligand
Short name=NGL-2
Gene names
Name:LRRC4
Synonyms:BAG
ORF Names:NAG14, UNQ554/PRO1111
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length653 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Synaptic adhesion protein. Regulates the formation of exitatory synapses through the recruitment of pre-and-postsynaptic proteins. Organize the lamina/pathway-specific differentiation of dendrites. Plays a important role for auditory synaptic responses. Involved in the suppression of glioma By similarity.

Subunit structure

Interacts with DLG4 By similarity. Interacts (via LRR repeats) with NTNG2. Forms a complex with DLG4 and with NMDA receptors.

Subcellular location

Membrane; Single-pass type I membrane protein. Cell junctionsynapsepostsynaptic cell membrane By similarity. Note: LRRC4 and DLG4 are interdependent for synaptic localization By similarity.

Tissue specificity

Specifically expressed in brain. Ref.1

Domain

The last 4 C-terminal residues bind to the first 2 PDZ domains of DLG4 By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Contains 1 Ig-like (immunoglobulin-like) domain.

Contains 9 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 LRRNT domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3838 Potential
Chain39 – 653615Leucine-rich repeat-containing protein 4
PRO_0000014833

Regions

Topological domain39 – 527489Extracellular Potential
Transmembrane528 – 54821Helical; Potential
Topological domain549 – 653105Cytoplasmic Potential
Domain39 – 7537LRRNT
Repeat76 – 9722LRR 1
Repeat100 – 12122LRR 2
Repeat124 – 14522LRR 3
Repeat148 – 16922LRR 4
Repeat172 – 19423LRR 5
Repeat197 – 21822LRR 6
Repeat219 – 24022LRR 7
Repeat243 – 26422LRR 8
Repeat267 – 28822LRR 9
Domain300 – 35253LRRCT
Domain353 – 44290Ig-like
Compositional bias455 – 52672Thr-rich

Amino acid modifications

Glycosylation2771N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3631N-linked (GlcNAc...) Potential
Glycosylation3881N-linked (GlcNAc...) Potential
Glycosylation4101N-linked (GlcNAc...) Potential
Glycosylation4341N-linked (GlcNAc...) Potential
Glycosylation4401N-linked (GlcNAc...) Potential
Glycosylation4471N-linked (GlcNAc...) Potential
Glycosylation4501N-linked (GlcNAc...) Potential
Disulfide bond46 ↔ 52 Ref.8
Disulfide bond50 ↔ 61 Ref.8
Disulfide bond304 ↔ 329 Ref.8
Disulfide bond306 ↔ 350 Ref.8
Disulfide bond374 ↔ 424 Ref.8

Natural variations

Natural variant5791T → A in a colorectal cancer sample; somatic mutation. Ref.9
VAR_035519

Experimental info

Sequence conflict41L → S in BAD18737. Ref.4
Sequence conflict253 – 2575QVSLI → H in CAC82651. Ref.2
Sequence conflict3001N → D in BAD18737. Ref.4
Sequence conflict3151L → F in BAD18737. Ref.4

Secondary structure

......................................................................... 653
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9HBW1 [UniParc].

Last modified October 1, 2001. Version 2.
Checksum: 38159C81F6850E37

FASTA65372,717
        10         20         30         40         50         60 
MKLLWQVTVH HHTWNAILLP FVYLTAQVWI LCAAIAAAAS AGPQNCPSVC SCSNQFSKVV 

        70         80         90        100        110        120 
CTRRGLSEVP QGIPSNTRYL NLMENNIQMI QADTFRHLHH LEVLQLGRNS IRQIEVGAFN 

       130        140        150        160        170        180 
GLASLNTLEL FDNWLTVIPS GAFEYLSKLR ELWLRNNPIE SIPSYAFNRV PSLMRLDLGE 

       190        200        210        220        230        240 
LKKLEYISEG AFEGLFNLKY LNLGMCNIKD MPNLTPLVGL EELEMSGNHF PEIRPGSFHG 

       250        260        270        280        290        300 
LSSLKKLWVM NSQVSLIERN AFDGLASLVE LNLAHNNLSS LPHDLFTPLR YLVELHLHHN 

       310        320        330        340        350        360 
PWNCDCDILW LAWWLREYIP TNSTCCGRCH APMHMRGRYL VEVDQASFQC SAPFIMDAPR 

       370        380        390        400        410        420 
DLNISEGRMA ELKCRTPPMS SVKWLLPNGT VLSHASRHPR ISVLNDGTLN FSHVLLSDTG 

       430        440        450        460        470        480 
VYTCMVTNVA GNSNASAYLN VSTAELNTSN YSFFTTVTVE TTEISPEDTT RKYKPVPTTS 

       490        500        510        520        530        540 
TGYQPAYTTS TTVLIQTTRV PKQVAVPATD TTDKMQTSLD EVMKTTKIII GCFVAVTLLA 

       550        560        570        580        590        600 
AAMLIVFYKL RKRHQQRSTV TAARTVEIIQ VDEDIPAATS AAATAAPSGV SGEGAVVLPT 

       610        620        630        640        650 
IHDHINYNTY KPAHGAHWTE NSLGNSLHPT VTTISEPYII QTHTKDKVQE TQI 

« Hide

References

« Hide 'large scale' references
[1]"Expression and functional characterization of LRRC4, a novel brain-specific member of the LRR superfamily."
Zhang Q., Wang J., Fan S., Wang L., Cao L., Tang K., Peng C., Li Z., Li W., Gan K., Liu Z., Li X., Shen S., Li G.
FEBS Lett. 579:3674-3682(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]Wang J.
Thesis (2000), Zhongshan Medical University / Guangzhou, China
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Solution structure of the Ig-like domain of human leucine-rich repeat-containing protein 4."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 351-442.
[8]"Structural basis for cell surface patterning through NetrinG-NGL interactions."
Seiradake E., Coles C.H., Perestenko P.V., Harlos K., McIlhinney R.A., Aricescu A.R., Jones E.Y.
EMBO J. 30:4479-4488(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 1-444 IN COMPLEX WITH NTNG2, DISULFIDE BONDS.
[9]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF196976 mRNA. Translation: AAG28019.2.
AJ297858 mRNA. Translation: CAC82651.1.
AY358307 mRNA. Translation: AAQ88674.1.
AK172751 mRNA. Translation: BAD18737.1.
AK314047 mRNA. Translation: BAG36756.1.
CH236947 Genomic DNA. Translation: EAL24316.1.
BC111561 mRNA. Translation: AAI11562.1.
BC111745 mRNA. Translation: AAI11746.1.
RefSeqNP_071426.1. NM_022143.4.
UniGeneHs.655003.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DL9NMR-A353-442[»]
3ZYIX-ray2.60A1-444[»]
ProteinModelPortalQ9HBW1.
SMRQ9HBW1. Positions 44-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122061. 1 interaction.
IntActQ9HBW1. 3 interactions.
MINTMINT-6489320.
STRING9606.ENSP00000249363.

Protein family/group databases

MEROPSI43.001.

PTM databases

PhosphoSiteQ9HBW1.

Polymorphism databases

DMDM51701696.

Proteomic databases

PaxDbQ9HBW1.
PRIDEQ9HBW1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000249363; ENSP00000249363; ENSG00000128594.
GeneID64101.
KEGGhsa:64101.
UCSCuc003vmk.3. human.

Organism-specific databases

CTD64101.
GeneCardsGC07M127667.
HGNCHGNC:15586. LRRC4.
HPAHPA051100.
MIM610486. gene.
neXtProtNX_Q9HBW1.
PharmGKBPA30463.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4886.
HOGENOMHOG000252924.
HOVERGENHBG052359.
InParanoidQ9HBW1.
KOK16351.
OMACGCEAVW.
OrthoDBEOG769ZHZ.
PhylomeDBQ9HBW1.
TreeFamTF324303.

Gene expression databases

ArrayExpressQ9HBW1.
BgeeQ9HBW1.
CleanExHS_LRRC4.
GenevestigatorQ9HBW1.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR026906. LRR_5.
IPR026882. Lrrc4.
[Graphical view]
PANTHERPTHR24369:SF9. PTHR24369:SF9. 1 hit.
PfamPF07679. I-set. 1 hit.
PF13306. LRR_5. 1 hit.
[Graphical view]
SMARTSM00408. IGc2. 1 hit.
SM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
PS51450. LRR. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9HBW1.
GeneWikiLRRC4.
GenomeRNAi64101.
NextBio65940.
PROQ9HBW1.
SOURCESearch...

Entry information

Entry nameLRRC4_HUMAN
AccessionPrimary (citable) accession number: Q9HBW1
Secondary accession number(s): A4D0Y9 expand/collapse secondary AC list , Q14DU9, Q6ZMI8, Q96A85
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: October 1, 2001
Last modified: February 19, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM