Lysophosphatidic acid receptor 2 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot Q9HBW0 (LPAR2_HUMAN)

Last modified June 16, 2009. Version 71. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Lysophosphatidic acid receptor 2
      Short name=LPA receptor 2
      Short name=LPA-2
Alternative name(s):
    Lysophosphatidic acid receptor Edg-4

Gene names

Name:	LPAR2
Synonyms:	EDG4, LPA2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Ref.9
Subunit structure	Interacts with SLC9A3R2/NHERF2, MAGI3 and PLCB3. Ref.9 Ref.10
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon.
Miscellaneous	Ref.1 cDNA clone has a guanine nucleotide deletion that causes a frameshift near its C-terminal coding region. This likely reflects a somatic mutation in the ovary tumor cells from which the cDNA was isolated and may have altered the function of the encoded receptor, and contributed to transformation of the original ovary cells that formed a tumor.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAC27728.1 differs from that shown. Reason: Frameshift at position 348.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Ref.1 Traceable author statement. Source: ProtInc activation of phospholipase C activity Traceable author statement. Source: ProtInc elevation of cytosolic calcium ion concentration Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	LIM domain binding Inferred from physical interaction. Source: UniProtKB lipid binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
CFTR	P13569	1	EBI-765995,EBI-349854
SLC9A3R2	Q15599	1	EBI-765995,EBI-1149760

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 351

351

Lysophosphatidic acid receptor 2

PRO_0000069424

Regions

Topological domain

1 – 33

Extracellular Potential

Transmembrane

34 – 54

1 Potential

Topological domain

55 – 69

Cytoplasmic Potential

Transmembrane

70 – 90

2 Potential

Topological domain

91 – 103

Extracellular Potential

Transmembrane

104 – 126

3 Potential

Topological domain

127 – 146

Cytoplasmic Potential

Transmembrane

147 – 167

4 Potential

Topological domain

168 – 188

Extracellular Potential

Transmembrane

189 – 209

5 Potential

Topological domain

210 – 242

Cytoplasmic Potential

Transmembrane

243 – 263

6 Potential

Topological domain

264 – 279

Extracellular Potential

Transmembrane

280 – 297

7 Potential

Topological domain

298 – 351

Cytoplasmic Potential

Motif

348 – 351

PDZ-binding

Amino acid modifications

Lipidation

311

S-palmitoyl cysteine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis

348

D → A: Abolishes interaction with MAGI3. Ref.10

Mutagenesis

349

S → A: Abolishes interaction with MAGI3. Ref.10

Mutagenesis

350

T → A: Does not affect interaction with MAGI3. Ref.10

Mutagenesis

351

L → A: Abolishes interaction with MAGI3. Ref.10

Sequence conflict

221

R → S in AAG28521. Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9HBW0-1 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: DE225B0B3E9351B8
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FASTA

351

39,084

        10         20         30         40         50         60 
MVIMGQCYYN ETIGFFYNNS GKELSSHWRP KDVVVVALGL TVSVLVLLTN LLVIAAIASN 

        70         80         90        100        110        120 
RRFHQPIYYL LGNLAAADLF AGVAYLFLMF HTGPRTARLS LEGWFLRQGL LDTSLTASVA 

       130        140        150        160        170        180 
TLLAIAVERH RSVMAVQLHS RLPRGRVVML IVGVWVAALG LGLLPAHSWH CLCALDRCSR 

       190        200        210        220        230        240 
MAPLLSRSYL AVWALSSLLV FLLMVAVYTR IFFYVRRRVQ RMAEHVSCHP RYRETTLSLV 

       250        260        270        280        290        300 
KTVVIILGAF VVCWTPGQVV LLLDGLGCES CNVLAVEKYF LLLAEANSLV NAAVYSCRDA 

       310        320        330        340        350 
EMRRTFRRLL CCACLRQSTR ESVHYTSSAQ GGASTRIMLP ENGHPLMDST L

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid." An S., Bleu T., Hallmark O.G., Goetzl E.J. J. Biol. Chem. 273:7906-7910(1998) [PubMed: 9525886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovarian carcinoma.
[2]	"Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species. Structure-activity relationship of cloned LPA receptors." Bandoh K., Aoki J., Taira A., Tsujimoto M., Arai H., Inoue K. FEBS Lett. 478:159-165(2000) [PubMed: 10922489] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Human Edg4 lysophosphatidic acid receptor cDNA encoding a putative protein with COOH-terminus different from the previously-reported Edg4." An S. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon cancer.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., King M.M., Cismowski M.J., Aronstam R.S., Sharma S.V. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[7]	"Lysophosphatidic acid receptors." Contos J.J.A., Ishii I., Chun J. Mol. Pharmacol. 58:1188-1196(2000) [PubMed: 11093753] [Abstract] Cited for: REVIEW.
[8]	"Genomic characterization of the lysophosphatidic acid receptor gene, lp(A2)/Edg4, and identification of a frameshift mutation in a previously characterized cDNA." Contos J.J.A., Chun J. Genomics 64:155-169(2000) [PubMed: 10729222] [Abstract] Cited for: IDENTIFICATION OF A PROBABLE FRAMESHIFT MUTATION.
[9]	"NHERF2 specifically interacts with LPA2 receptor and defines the specificity and efficiency of receptor-mediated phospholipase C-beta3 activation." Oh Y.-S., Jo N.W., Choi J.W., Kim H.S., Seo S.-W., Kang K.-O., Hwang J.-I., Heo K., Kim S.-H., Kim Y.-H., Kim I.-H., Kim J.H., Banno Y., Ryu S.H., Suh P.-G. Mol. Cell. Biol. 24:5069-5079(2004) [PubMed: 15143197] [Abstract] Cited for: FUNCTION, INTERACTION WITH SLC9A3R2/NHERF2 AND PLCB3.
[10]	"MAGI-3 regulates LPA-induced activation of Erk and RhoA." Zhang H., Wang D., Sun H., Hall R.A., Yun C.C. Cell. Signal. 19:261-268(2007) [PubMed: 16904289] [Abstract] Cited for: INTERACTION WITH MAGI3, MUTAGENESIS OF ASP-348; SER-349; THR-350 AND LEU-351.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF011466 mRNA. Translation: AAC27728.1. Frameshift. AF233092 mRNA. Translation: AAF43409.1. AF197929 mRNA. Translation: AAG28521.1. AY322548 mRNA. Translation: AAP84361.1. AC002306 Genomic DNA. Translation: AAB61528.1. BC025695 mRNA. Translation: AAH25695.1.
IPI	IPI00172675.
RefSeq	NP_004711.2.
UniGene	Hs.122575
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HBW0. 3 interactions.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9HBW0.
Proteomic databases
PeptideAtlas	Q9HBW0.
PRIDE	Q9HBW0.
Genome annotation databases
Ensembl	ENSG00000064547. Homo sapiens. [Contig view]
GeneID	9170.
KEGG	hsa:9170.
Organism-specific databases
GeneCards	GC19M019597.
H-InvDB	HIX0014946.
HGNC	HGNC:3168. LPAR2.
MIM	605110. gene.
PharmGKB	PA27608.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9HBW0.
HOVERGEN	Q9HBW0.
OMA	Q9HBW0. CKSCNVL.
Enzyme and pathway databases
Pathway_Interaction_DB	lysophospholipid_pathway. LPA receptor mediated events.
Gene expression databases
ArrayExpress	Q9HBW0.
Bgee	Q9HBW0.
CleanEx	HS_LPAR2.
GermOnline	ENSG00000064547. Homo sapiens.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR004065. LPA_rcpt. IPR004066. LPA_rcpt_EDG4. [Graphical view]
PANTHER	PTHR22750:SF12. LPA_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01528. EDG4RECEPTOR. PR00237. GPCRRHODOPSN. PR01527. LPARECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	34391.
SOURCE	Search...

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Entry information

Entry name

LPAR2_HUMAN

Accession

Primary (citable) accession number: Q9HBW0
Secondary accession number(s): O00543, O43431

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	December 6, 2002
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents