Q9HBU6 (EKI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ethanolamine kinase 1
Short name=EKI 1
EC=2.7.1.82

Gene names

Name:	ETNK1
Synonyms:	EKI1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis.
Catalytic activity	ATP + ethanolamine = ADP + O-phosphoethanolamine.
Pathway	Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in kidney, liver, placenta, heart, leukocyte, ovary and testis.
Sequence similarities	Belongs to the choline/ethanolamine kinase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphatidylethanolamine biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	cytoplasm Non-traceable author statement. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ethanolamine kinase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Ethanolamine kinase 1		PRO_0000206227
Regions
	Compositional bias	73 – 83	11	Poly-Val
Amino acid modifications
	Modified residue	449	1	N6-acetyllysine Ref.3
Experimental info
	Sequence conflict	228 – 258	31	RLIAR…LKMGK → SLSSLTLCKGKTTRCFGLTG CRGSRLLLSFF in AAH06111. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9HBU6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9AF29EAC556ED91F	FASTA	452	50,968
	10 20 30 40 50 60 MLCGRPRSSS DNRNFLRERA GLSSAAVQTR IGNSAASRRS PAARPPVPAP PALPRGRPGT 70 80 90 100 110 120 EGSTSLSAPA VLVVAVAVVV VVVSAVAWAM ANYIHVPPGS PEVPKLNVTV QDQEEHRCRE 130 140 150 160 170 180 GALSLLQHLR PHWDPQEVTL QLFTDGITNK LIGCYVGNTM EDVVLVRIYG NKTELLVDRD 190 200 210 220 230 240 EEVKSFRVLQ AHGCAPQLYC TFNNGLCYEF IQGEALDPKH VCNPAIFRLI ARQLAKIHAI 250 260 270 280 290 300 HAHNGWIPKS NLWLKMGKYF SLIPTGFADE DINKRFLSDI PSSQILQEEM TWMKEILSNL 310 320 330 340 350 360 GSPVVLCHND LLCKNIIYNE KQGDVQFIDY EYSGYNYLAY DIGNHFNEFA GVSDVDYSLY 370 380 390 400 410 420 PDRELQSQWL RAYLEAYKEF KGFGTEVTEK EVEILFIQVN QFALASHFFW GLWALIQAKY 430 440 450 STIEFDFLGY AIVRFNQYFK MKPEVTALKV PE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overexpression of a mammalian ethanolamine-specific kinase accelerates the CDP-ethanolamine pathway." Lykidis A., Wang J., Karim M.A., Jackowski S. J. Biol. Chem. 276:2174-2179(2001) [PubMed: 11044454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-449, MASS SPECTROMETRY.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF207600 mRNA. Translation: AAF71220.2. BC006111 mRNA. Translation: AAH06111.2. BC066907 mRNA. Translation: AAH66907.1.
IPI	IPI00030090.
RefSeq	NP_001034570.1. NM_001039481.1. NP_061108.2. NM_018638.4.
UniGene	Hs.29464.
3D structure databases
ProteinModelPortal	Q9HBU6.
SMR	Q9HBU6. Positions 113-445.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HBU6. 1 interaction.
STRING	Q9HBU6.
PTM databases
PhosphoSite	Q9HBU6.
Polymorphism databases
DMDM	14194724.
Proteomic databases
PRIDE	Q9HBU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000266517; ENSP00000266517; ENSG00000139163.
GeneID	55500.
KEGG	hsa:55500.
UCSC	uc001rft.1. human.
Organism-specific databases
CTD	55500.
GeneCards	GC12P022778.
H-InvDB	HIX0010485.
HGNC	HGNC:24649. ETNK1.
MIM	609858. gene.
neXtProt	NX_Q9HBU6.
PharmGKB	PA134921265.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00530000062991.
HOGENOM	HBG713177.
HOVERGEN	HBG018981.
InParanoid	Q9HBU6.
OMA	EVLYVQV.
OrthoDB	EOG4HT8S7.
PhylomeDB	Q9HBU6.
Enzyme and pathway databases
BRENDA	2.7.1.82. 2681.
Gene expression databases
ArrayExpress	Q9HBU6.
Bgee	Q9HBU6.
CleanEx	HS_ETNK1.
Genevestigator	Q9HBU6.
GermOnline	ENSG00000139163. Homo sapiens.
Family and domain databases
InterPro	IPR002573. Choline/ethanolamine_kinase. IPR011009. Kinase-like_dom. [Graphical view]
KO	K00894.
Pfam	PF01633. Choline_kinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
ProtoNet	Search...
Other
NextBio	59873.
SOURCE	Search...

Entry information

Entry name

EKI1_HUMAN

Accession

Primary (citable) accession number: Q9HBU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.