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Protein

Kinetochore protein Spc25

Gene

SPC25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).3 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB
  • mitotic cell cycle Source: Reactome
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle organization Source: UniProtKB
  • small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Spc25
Short name:
hSpc25
Gene namesi
Name:SPC25
Synonyms:SPBC25
ORF Names:AD024
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:24031. SPC25.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB
  • cytosol Source: Reactome
  • Ndc80 complex Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162404413.

Polymorphism and mutation databases

BioMutaiSPC25.
DMDMi74734256.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Kinetochore protein Spc25PRO_0000249565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9HBM1.
MaxQBiQ9HBM1.
PaxDbiQ9HBM1.
PeptideAtlasiQ9HBM1.
PRIDEiQ9HBM1.

PTM databases

iPTMnetiQ9HBM1.
PhosphoSiteiQ9HBM1.

Expressioni

Gene expression databases

BgeeiQ9HBM1.
CleanExiHS_SPC25.
ExpressionAtlasiQ9HBM1. baseline and differential.
GenevisibleiQ9HBM1. HS.

Organism-specific databases

HPAiHPA047144.
HPA050544.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NDC80O1477710EBI-999909,EBI-715849
SPC24Q8NBT26EBI-999909,EBI-999900

Protein-protein interaction databases

BioGridi121507. 39 interactions.
DIPiDIP-35753N.
IntActiQ9HBM1. 17 interactions.
MINTiMINT-4786938.
STRINGi9606.ENSP00000282074.

Structurei

Secondary structure

1
224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi118 – 1258Combined sources
Turni126 – 1305Combined sources
Helixi131 – 14111Combined sources
Beta strandi154 – 1574Combined sources
Beta strandi171 – 1755Combined sources
Beta strandi177 – 1804Combined sources
Helixi195 – 2039Combined sources
Helixi207 – 2148Combined sources
Helixi216 – 2194Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88A/B118-223[»]
3IZ0electron microscopy-C/E118-223[»]
ProteinModelPortaliQ9HBM1.
SMRiQ9HBM1. Positions 59-223.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HBM1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 144144Interaction with the N-terminus of SPBC24Add
BLAST
Regioni1 – 5656Interaction with the NDC80-NUF2 subcomplexAdd
BLAST
Regioni145 – 22480Interaction with the C-terminus of SPBC24Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili61 – 14282Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the SPC25 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4657. Eukaryota.
ENOG4111Y5R. LUCA.
GeneTreeiENSGT00390000002220.
HOGENOMiHOG000231978.
HOVERGENiHBG080509.
InParanoidiQ9HBM1.
KOiK11550.
OMAiDTSCQMV.
PhylomeDBiQ9HBM1.
TreeFamiTF332941.

Family and domain databases

InterProiIPR013255. Spc25.
[Graphical view]
PfamiPF08234. Spindle_Spc25. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HBM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK
60 70 80 90 100
LKEEERMVEM FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT
110 120 130 140 150
ANIQDLKEEY SRKKETISTA NKANAERLKR LQKSADLYKD RLGLEIRKIY
160 170 180 190 200
GEKLQFIFTN IDPKNPESPF MFSLHLNEAR DYEVSDSAPH LEGLAEFQEN
210 220
VRKTNNFSAF LANVRKAFTA TVYN
Length:224
Mass (Da):26,153
Last modified:March 1, 2001 - v1
Checksum:i6DED3216794F4512
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY456388 mRNA. Translation: AAR88652.1.
AF225416 mRNA. Translation: AAG09718.1.
CR457197 mRNA. Translation: CAG33478.1.
AK291093 mRNA. Translation: BAF83782.1.
AC069137 Genomic DNA. Translation: AAY24098.1.
CH471058 Genomic DNA. Translation: EAX11289.1.
CH471058 Genomic DNA. Translation: EAX11290.1.
BC022255 mRNA. Translation: AAH22255.1.
CCDSiCCDS2229.1.
RefSeqiNP_065726.1. NM_020675.3.
UniGeneiHs.421956.

Genome annotation databases

EnsembliENST00000282074; ENSP00000282074; ENSG00000152253.
ENST00000611144; ENSP00000481354; ENSG00000277909.
ENST00000616584; ENSP00000483050; ENSG00000277909.
GeneIDi57405.
KEGGihsa:57405.
UCSCiuc002uel.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY456388 mRNA. Translation: AAR88652.1.
AF225416 mRNA. Translation: AAG09718.1.
CR457197 mRNA. Translation: CAG33478.1.
AK291093 mRNA. Translation: BAF83782.1.
AC069137 Genomic DNA. Translation: AAY24098.1.
CH471058 Genomic DNA. Translation: EAX11289.1.
CH471058 Genomic DNA. Translation: EAX11290.1.
BC022255 mRNA. Translation: AAH22255.1.
CCDSiCCDS2229.1.
RefSeqiNP_065726.1. NM_020675.3.
UniGeneiHs.421956.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88A/B118-223[»]
3IZ0electron microscopy-C/E118-223[»]
ProteinModelPortaliQ9HBM1.
SMRiQ9HBM1. Positions 59-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121507. 39 interactions.
DIPiDIP-35753N.
IntActiQ9HBM1. 17 interactions.
MINTiMINT-4786938.
STRINGi9606.ENSP00000282074.

PTM databases

iPTMnetiQ9HBM1.
PhosphoSiteiQ9HBM1.

Polymorphism and mutation databases

BioMutaiSPC25.
DMDMi74734256.

Proteomic databases

EPDiQ9HBM1.
MaxQBiQ9HBM1.
PaxDbiQ9HBM1.
PeptideAtlasiQ9HBM1.
PRIDEiQ9HBM1.

Protocols and materials databases

DNASUi57405.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282074; ENSP00000282074; ENSG00000152253.
ENST00000611144; ENSP00000481354; ENSG00000277909.
ENST00000616584; ENSP00000483050; ENSG00000277909.
GeneIDi57405.
KEGGihsa:57405.
UCSCiuc002uel.4. human.

Organism-specific databases

CTDi57405.
GeneCardsiSPC25.
HGNCiHGNC:24031. SPC25.
HPAiHPA047144.
HPA050544.
MIMi609395. gene.
neXtProtiNX_Q9HBM1.
PharmGKBiPA162404413.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4657. Eukaryota.
ENOG4111Y5R. LUCA.
GeneTreeiENSGT00390000002220.
HOGENOMiHOG000231978.
HOVERGENiHBG080509.
InParanoidiQ9HBM1.
KOiK11550.
OMAiDTSCQMV.
PhylomeDBiQ9HBM1.
TreeFamiTF332941.

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.

Miscellaneous databases

EvolutionaryTraceiQ9HBM1.
GenomeRNAii57405.
NextBioi63516.
PROiQ9HBM1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9HBM1.
CleanExiHS_SPC25.
ExpressionAtlasiQ9HBM1. baseline and differential.
GenevisibleiQ9HBM1. HS.

Family and domain databases

InterProiIPR013255. Spc25.
[Graphical view]
PfamiPF08234. Spindle_Spc25. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment."
    McCleland M.L., Kallio M.J., Barrett-Wilt G.A., Kestner C.A., Shabanowitz J., Hunt D.F., Gorbsky G.J., Stukenberg P.T.
    Curr. Biol. 14:131-137(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. Xiao H., Song H., Gao G., Ren S., Chen Z., Han Z.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Adrenal gland.
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  8. "Identification of two novel components of the human NDC80 kinetochore complex."
    Bharadwaj R., Qi W., Yu H.
    J. Biol. Chem. 279:13076-13085(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NDC80 COMPLEX, SUBCELLULAR LOCATION.
  9. "Architecture of the human Ndc80-Hec1 complex, a critical constituent of the outer kinetochore."
    Ciferri C., De Luca J., Monzani S., Ferrari K.J., Ristic D., Wyman C., Stark H., Kilmartin J., Salmon E.D., Musacchio A.
    J. Biol. Chem. 280:29088-29095(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments."
    Schmidt J.C., Arthanari H., Boeszoermenyi A., Dashkevich N.M., Wilson-Kubalek E.M., Monnier N., Markus M., Oberer M., Milligan R.A., Bathe M., Wagner G., Grishchuk E.L., Cheeseman I.M.
    Dev. Cell 23:968-980(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPC25_HUMAN
AccessioniPrimary (citable) accession number: Q9HBM1
Secondary accession number(s): A8K4X8, D3DPC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2001
Last modified: April 13, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.