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Q9HBK9

- AS3MT_HUMAN

UniProt

Q9HBK9 - AS3MT_HUMAN

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Protein

Arsenite methyltransferase

Gene

AS3MT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from AdoMet to trivalent arsenicals producing methylated and dimethylated arsenicals. It methylates arsenite to form methylarsonate, Me-AsO3H2, which is reduced by methylarsonate reductase to methylarsonite, Me-As(OH)2. Methylarsonite is also a substrate and it is converted into the much less toxic compound dimethylarsinate (cacodylate), Me2As(O)-OH (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate.
S-adenosyl-L-methionine + methylarsonite = S-adenosyl-L-homocysteine + dimethylarsinate.

Kineticsi

  1. KM=4.6 µM for sodium arsenite1 Publication
  2. KM=11.8 µM for AdoMet1 Publication

GO - Molecular functioni

  1. arsenite methyltransferase activity Source: UniProtKB
  2. methylarsonite methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. arsonoacetate metabolic process Source: UniProtKB
  2. toxin metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:HS01822-MONOMER.
BRENDAi2.1.1.137. 2681.
SABIO-RKQ9HBK9.

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite methyltransferase (EC:2.1.1.137)
Alternative name(s):
Methylarsonite methyltransferase
S-adenosyl-L-methionine:arsenic(III) methyltransferase
Gene namesi
Name:AS3MT
Synonyms:CYT19
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 10

Organism-specific databases

HGNCiHGNC:17452. AS3MT.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134896392.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Arsenite methyltransferasePRO_0000204447Add
BLAST

Proteomic databases

MaxQBiQ9HBK9.
PaxDbiQ9HBK9.
PRIDEiQ9HBK9.

PTM databases

PhosphoSiteiQ9HBK9.

Expressioni

Gene expression databases

BgeeiQ9HBK9.
CleanExiHS_AS3MT.
GenevestigatoriQ9HBK9.

Organism-specific databases

HPAiHPA017856.
HPA027708.

Interactioni

Protein-protein interaction databases

BioGridi121513. 1 interaction.
IntActiQ9HBK9. 2 interactions.
STRINGi9606.ENSP00000358896.

Structurei

3D structure databases

ProteinModelPortaliQ9HBK9.
SMRiQ9HBK9. Positions 39-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiNOG257055.
GeneTreeiENSGT00390000001742.
HOGENOMiHOG000229966.
HOVERGENiHBG050585.
InParanoidiQ9HBK9.
KOiK07755.
OMAiDIVVSNC.
OrthoDBiEOG7V49ZQ.
PhylomeDBiQ9HBK9.
TreeFamiTF343797.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR026669. Arsenite_MeTrfase.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10108:SF11. PTHR10108:SF11. 1 hit.
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: An isoform missing exon 3 is produced with a premature stop codon at AA 23 and is targeted to nonsense-mediated mRNA decay (NMD).

Isoform 1 (identifier: Q9HBK9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALRDAEIQ KDVQTYYGQV LKRSADLQTN GCVTTARPVP KHIREALQNV
60 70 80 90 100
HEEVALRYYG CGLVIPEHLE NCWILDLGSG SGRDCYVLSQ LVGEKGHVTG
110 120 130 140 150
IDMTKGQVEV AEKYLDYHME KYGFQASNVT FIHGYIEKLG EAGIKNESHD
160 170 180 190 200
IVVSNCVINL VPDKQQVLQE AYRVLKHGGE LYFSDVYTSL ELPEEIRTHK
210 220 230 240 250
VLWGECLGGA LYWKELAVLA QKIGFCPPRL VTANLITIQN KELERVIGDC
260 270 280 290 300
RFVSATFRLF KHSKTGPTKR CQVIYNGGIT GHEKELMFDA NFTFKEGEIV
310 320 330 340 350
EVDEETAAIL KNSRFAQDFL IRPIGEKLPT SGGCSALELK DIITDPFKLA
360 370
EESDSMKSRC VPDAAGGCCG TKKSC
Length:375
Mass (Da):41,748
Last modified:October 14, 2008 - v3
Checksum:i2E0C758A8597AE33
GO
Isoform 2 (identifier: Q9HBK9-2) [UniParc]FASTAAdd to Basket

Also known as: 31.1 kDa, delta4,5

The sequence of this isoform differs from the canonical sequence as follows:
     58-153: Missing.

Note: Devoid of methyltransferase activity.

Show »
Length:279
Mass (Da):31,098
Checksum:i50160483AE264D85
GO

Sequence cautioni

The sequence AAG09731.1 differs from that shown. Reason: Frameshift at position 333. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251Q → R in AAI19638. (PubMed:15489334)Curated
Sequence conflicti132 – 1321I → F in AAG09731. 1 PublicationCurated
Sequence conflicti135 – 1351Y → N in AAG09731. 1 PublicationCurated
Sequence conflicti140 – 1401G → A in AAG09731. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti173 – 1731R → W Rare polymorphism; frequency in African-Americans 0.008; not detected in Caucasian-Americans; enzyme activity is 31% of wild-type. 1 Publication
Corresponds to variant rs35232887 [ dbSNP | Ensembl ].
VAR_027392
Natural varianti287 – 2871M → T Common polymorphism; frequency in African-Americans 0.108 and Caucasian-Americans 0.100; enzyme activity is 350% of wild-type. 2 Publications
Corresponds to variant rs11191439 [ dbSNP | Ensembl ].
VAR_027393
Natural varianti306 – 3061T → I Rare polymorphism; frequency in Caucasian-Americans 0.008; not detected in African-Americans. 1 Publication
Corresponds to variant rs34556438 [ dbSNP | Ensembl ].
VAR_027394

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei58 – 15396Missing in isoform 2. CuratedVSP_053494Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226730 mRNA. Translation: AAG09731.1. Frameshift.
AY817668 Genomic DNA. Translation: AAV68045.1.
AL358790 Genomic DNA. Translation: CAI52503.1.
CH471066 Genomic DNA. Translation: EAW49668.1.
BC119637 mRNA. Translation: AAI19638.1.
BC119638 mRNA. Translation: AAI19639.2.
CCDSiCCDS41567.1. [Q9HBK9-1]
RefSeqiNP_065733.2. NM_020682.3. [Q9HBK9-1]
UniGeneiHs.720370.

Genome annotation databases

EnsembliENST00000369880; ENSP00000358896; ENSG00000214435. [Q9HBK9-1]
GeneIDi57412.
KEGGihsa:57412.
UCSCiuc001kwj.3. human. [Q9HBK9-1]

Polymorphism databases

DMDMi209572762.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226730 mRNA. Translation: AAG09731.1 . Frameshift.
AY817668 Genomic DNA. Translation: AAV68045.1 .
AL358790 Genomic DNA. Translation: CAI52503.1 .
CH471066 Genomic DNA. Translation: EAW49668.1 .
BC119637 mRNA. Translation: AAI19638.1 .
BC119638 mRNA. Translation: AAI19639.2 .
CCDSi CCDS41567.1. [Q9HBK9-1 ]
RefSeqi NP_065733.2. NM_020682.3. [Q9HBK9-1 ]
UniGenei Hs.720370.

3D structure databases

ProteinModelPortali Q9HBK9.
SMRi Q9HBK9. Positions 39-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121513. 1 interaction.
IntActi Q9HBK9. 2 interactions.
STRINGi 9606.ENSP00000358896.

PTM databases

PhosphoSitei Q9HBK9.

Polymorphism databases

DMDMi 209572762.

Proteomic databases

MaxQBi Q9HBK9.
PaxDbi Q9HBK9.
PRIDEi Q9HBK9.

Protocols and materials databases

DNASUi 57412.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000369880 ; ENSP00000358896 ; ENSG00000214435 . [Q9HBK9-1 ]
GeneIDi 57412.
KEGGi hsa:57412.
UCSCi uc001kwj.3. human. [Q9HBK9-1 ]

Organism-specific databases

CTDi 57412.
GeneCardsi GC10P104629.
HGNCi HGNC:17452. AS3MT.
HPAi HPA017856.
HPA027708.
MIMi 611806. gene.
neXtProti NX_Q9HBK9.
PharmGKBi PA134896392.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG257055.
GeneTreei ENSGT00390000001742.
HOGENOMi HOG000229966.
HOVERGENi HBG050585.
InParanoidi Q9HBK9.
KOi K07755.
OMAi DIVVSNC.
OrthoDBi EOG7V49ZQ.
PhylomeDBi Q9HBK9.
TreeFami TF343797.

Enzyme and pathway databases

BioCyci MetaCyc:HS01822-MONOMER.
BRENDAi 2.1.1.137. 2681.
SABIO-RK Q9HBK9.

Miscellaneous databases

GeneWikii AS3MT.
GenomeRNAii 57412.
NextBioi 63542.
PROi Q9HBK9.
SOURCEi Search...

Gene expression databases

Bgeei Q9HBK9.
CleanExi HS_AS3MT.
Genevestigatori Q9HBK9.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR026669. Arsenite_MeTrfase.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10108:SF11. PTHR10108:SF11. 1 hit.
Pfami PF13847. Methyltransf_31. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human arsenic methyltransferase (AS3MT) pharmacogenetics: gene resequencing and functional genomics studies."
    Wood T.C., Salavagionne O.E., Mukherjee B., Wang L., Klumpp A.F., Thomae B.A., Eckloff B.W., Schaid D.J., Wieben E.D., Weinshilboum R.M.
    J. Biol. Chem. 281:7364-7373(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), BIOPHYSICOCHEMICAL PROPERTIES, VARIANTS TRP-173; THR-287 AND ILE-306.
  2. Xiao H., Song H., Gao G., Ren S., Chen Z., Han Z.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Adrenal tumor.
  3. Yu L., Kalla K., Guthrie E., Vidrine A., Klimecki W.T.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-367 (ISOFORM 1).
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Alternative splicing variants of human arsenic (+3 oxidation state) methyltransferase."
    Sumi D., Fukushima K., Miyataka H., Himeno S.
    Biochem. Biophys. Res. Commun. 415:48-53(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  9. Cited for: VARIANT THR-287.

Entry informationi

Entry nameiAS3MT_HUMAN
AccessioniPrimary (citable) accession number: Q9HBK9
Secondary accession number(s): A6NP79
, Q0VDK3, Q0VDK4, Q5PZ02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 14, 2008
Last modified: October 29, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3