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Protein

Rho-related GTP-binding protein RhoF

Gene

RHOF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 33GTPBy similarity8
Nucleotide bindingi73 – 77GTPBy similarity5
Nucleotide bindingi131 – 134GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoF
Alternative name(s):
Rho family GTPase Rif
Rho in filopodia
Gene namesi
Name:RHOF
Synonyms:ARHF, RIF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139725.7.
HGNCiHGNC:15703. RHOF.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi54509.
OpenTargetsiENSG00000139725.
PharmGKBiPA24954.

Polymorphism and mutation databases

BioMutaiRHOF.
DMDMi13633711.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988651 – 208Rho-related GTP-binding protein RhoFAdd BLAST208
PropeptideiPRO_0000281216209 – 211Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei208Cysteine methyl esterSequence analysis1
Lipidationi208S-geranylgeranyl cysteineSequence analysis1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ9HBH0.
MaxQBiQ9HBH0.
PaxDbiQ9HBH0.
PeptideAtlasiQ9HBH0.
PRIDEiQ9HBH0.

PTM databases

iPTMnetiQ9HBH0.
PhosphoSitePlusiQ9HBH0.

Expressioni

Gene expression databases

BgeeiENSG00000139725.
CleanExiHS_RHOF.
ExpressionAtlasiQ9HBH0. baseline and differential.
GenevisibleiQ9HBH0. HS.

Organism-specific databases

HPAiHPA045912.

Interactioni

Protein-protein interaction databases

BioGridi120004. 18 interactors.
IntActiQ9HBH0. 7 interactors.
MINTiMINT-5006634.
STRINGi9606.ENSP00000267205.

Structurei

3D structure databases

ProteinModelPortaliQ9HBH0.
SMRiQ9HBH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 56Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000119020.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ9HBH0.
KOiK07872.
OMAiITYMQGQ.
OrthoDBiEOG091G0KNI.
PhylomeDBiQ9HBH0.
TreeFamiTF331746.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51420. RHO. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HBH0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAPGALAQT AAPGPGRKEL KIVIVGDGGC GKTSLLMVYS QGSFPEHYAP
60 70 80 90 100
SVFEKYTASV TVGSKEVTLN LYDTAGQEDY DRLRPLSYQN THLVLICYDV
110 120 130 140 150
MNPTSYDNVL IKWFPEVTHF CRGIPMVLIG CKTDLRKDKE QLRKLRAAQL
160 170 180 190 200
EPITYMQGLS ACEQIRAALY LECSAKFREN VEDVFREAAK VALSALKKAQ
210
RQKKRRLCLL L
Length:211
Mass (Da):23,625
Last modified:March 1, 2001 - v1
Checksum:iAF82AE0CEDC34FAD
GO
Isoform 2 (identifier: Q9HBH0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-170: GLSACEQIRAALY → VGRGQDPGAQPWL
     171-211: Missing.

Note: No experimental confirmation available.
Show »
Length:170
Mass (Da):18,869
Checksum:i8537B7D6896029E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142L → P in BAA91034 (PubMed:14702039).Curated1
Sequence conflicti207L → P in BAA91034 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013571158 – 170GLSAC…RAALY → VGRGQDPGAQPWL in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_013572171 – 211Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239923 mRNA. Translation: AAG24952.1.
AK000254 mRNA. Translation: BAA91034.1.
BC018208 mRNA. Translation: AAH18208.1.
CCDSiCCDS9222.1. [Q9HBH0-1]
RefSeqiNP_061907.2. NM_019034.2. [Q9HBH0-1]
UniGeneiHs.707579.
Hs.717201.

Genome annotation databases

EnsembliENST00000267205; ENSP00000267205; ENSG00000139725. [Q9HBH0-1]
ENST00000537171; ENSP00000477391; ENSG00000139725. [Q9HBH0-2]
GeneIDi54509.
KEGGihsa:54509.
UCSCiuc001ubb.4. human. [Q9HBH0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRHOF_HUMAN
AccessioniPrimary (citable) accession number: Q9HBH0
Secondary accession number(s): Q8WVB1, Q9NXH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: September 27, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families