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Protein

Rho-related GTP-binding protein RhoF

Gene

RHOF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338GTPBy similarity
Nucleotide bindingi73 – 775GTPBy similarity
Nucleotide bindingi131 – 1344GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoF
Alternative name(s):
Rho family GTPase Rif
Rho in filopodia
Gene namesi
Name:RHOF
Synonyms:ARHF, RIF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:15703. RHOF.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24954.

Polymorphism and mutation databases

BioMutaiRHOF.
DMDMi13633711.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Rho-related GTP-binding protein RhoFPRO_0000198865Add
BLAST
Propeptidei209 – 2113Removed in mature formSequence analysisPRO_0000281216

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei208 – 2081Cysteine methyl esterSequence analysis
Lipidationi208 – 2081S-geranylgeranyl cysteineSequence analysis

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiQ9HBH0.
MaxQBiQ9HBH0.
PaxDbiQ9HBH0.
PRIDEiQ9HBH0.

PTM databases

iPTMnetiQ9HBH0.
PhosphoSiteiQ9HBH0.

Expressioni

Gene expression databases

BgeeiQ9HBH0.
CleanExiHS_RHOF.
ExpressionAtlasiQ9HBH0. baseline and differential.
GenevisibleiQ9HBH0. HS.

Organism-specific databases

HPAiHPA038675.
HPA060057.

Interactioni

Protein-protein interaction databases

BioGridi120004. 14 interactions.
IntActiQ9HBH0. 7 interactions.
MINTiMINT-5006634.
STRINGi9606.ENSP00000267205.

Structurei

3D structure databases

ProteinModelPortaliQ9HBH0.
SMRiQ9HBH0. Positions 21-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 569Effector regionSequence analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000119020.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ9HBH0.
KOiK07872.
OMAiILIGCKT.
OrthoDBiEOG74TX0S.
PhylomeDBiQ9HBH0.
TreeFamiTF331746.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HBH0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAPGALAQT AAPGPGRKEL KIVIVGDGGC GKTSLLMVYS QGSFPEHYAP
60 70 80 90 100
SVFEKYTASV TVGSKEVTLN LYDTAGQEDY DRLRPLSYQN THLVLICYDV
110 120 130 140 150
MNPTSYDNVL IKWFPEVTHF CRGIPMVLIG CKTDLRKDKE QLRKLRAAQL
160 170 180 190 200
EPITYMQGLS ACEQIRAALY LECSAKFREN VEDVFREAAK VALSALKKAQ
210
RQKKRRLCLL L
Length:211
Mass (Da):23,625
Last modified:March 1, 2001 - v1
Checksum:iAF82AE0CEDC34FAD
GO
Isoform 2 (identifier: Q9HBH0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-170: GLSACEQIRAALY → VGRGQDPGAQPWL
     171-211: Missing.

Note: No experimental confirmation available.
Show »
Length:170
Mass (Da):18,869
Checksum:i8537B7D6896029E4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti142 – 1421L → P in BAA91034 (PubMed:14702039).Curated
Sequence conflicti207 – 2071L → P in BAA91034 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei158 – 17013GLSAC…RAALY → VGRGQDPGAQPWL in isoform 2. 1 PublicationVSP_013571Add
BLAST
Alternative sequencei171 – 21141Missing in isoform 2. 1 PublicationVSP_013572Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239923 mRNA. Translation: AAG24952.1.
AK000254 mRNA. Translation: BAA91034.1.
BC018208 mRNA. Translation: AAH18208.1.
CCDSiCCDS9222.1. [Q9HBH0-1]
RefSeqiNP_061907.2. NM_019034.2. [Q9HBH0-1]
UniGeneiHs.707579.
Hs.717201.

Genome annotation databases

EnsembliENST00000267205; ENSP00000267205; ENSG00000139725. [Q9HBH0-1]
ENST00000537171; ENSP00000477391; ENSG00000139725. [Q9HBH0-2]
GeneIDi54509.
KEGGihsa:54509.
UCSCiuc001ubb.4. human. [Q9HBH0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239923 mRNA. Translation: AAG24952.1.
AK000254 mRNA. Translation: BAA91034.1.
BC018208 mRNA. Translation: AAH18208.1.
CCDSiCCDS9222.1. [Q9HBH0-1]
RefSeqiNP_061907.2. NM_019034.2. [Q9HBH0-1]
UniGeneiHs.707579.
Hs.717201.

3D structure databases

ProteinModelPortaliQ9HBH0.
SMRiQ9HBH0. Positions 21-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120004. 14 interactions.
IntActiQ9HBH0. 7 interactions.
MINTiMINT-5006634.
STRINGi9606.ENSP00000267205.

PTM databases

iPTMnetiQ9HBH0.
PhosphoSiteiQ9HBH0.

Polymorphism and mutation databases

BioMutaiRHOF.
DMDMi13633711.

Proteomic databases

EPDiQ9HBH0.
MaxQBiQ9HBH0.
PaxDbiQ9HBH0.
PRIDEiQ9HBH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000267205; ENSP00000267205; ENSG00000139725. [Q9HBH0-1]
ENST00000537171; ENSP00000477391; ENSG00000139725. [Q9HBH0-2]
GeneIDi54509.
KEGGihsa:54509.
UCSCiuc001ubb.4. human. [Q9HBH0-1]

Organism-specific databases

CTDi54509.
GeneCardsiRHOF.
HGNCiHGNC:15703. RHOF.
HPAiHPA038675.
HPA060057.
neXtProtiNX_Q9HBH0.
PharmGKBiPA24954.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000119020.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ9HBH0.
KOiK07872.
OMAiILIGCKT.
OrthoDBiEOG74TX0S.
PhylomeDBiQ9HBH0.
TreeFamiTF331746.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

GenomeRNAii54509.
PROiQ9HBH0.

Gene expression databases

BgeeiQ9HBH0.
CleanExiHS_RHOF.
ExpressionAtlasiQ9HBH0. baseline and differential.
GenevisibleiQ9HBH0. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The novel Rho-family GTPase rif regulates coordinated actin-based membrane rearrangements."
    Ellis S., Mellor H.
    Curr. Biol. 10:1387-1390(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon mucosa.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Ovary.
  4. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHOF_HUMAN
AccessioniPrimary (citable) accession number: Q9HBH0
Secondary accession number(s): Q8WVB1, Q9NXH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.