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Q9HBF4 (ZFYV1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger FYVE domain-containing protein 1
Alternative name(s):
Double FYVE-containing protein 1
SR3
Tandem FYVE fingers-1
Gene names
Name:ZFYVE1
Synonyms:DFCP1, KIAA1589, TAFF1, ZNFN2A1
ORF Names:PP10436
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length777 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger. Ref.2 Ref.3 Ref.9

Subcellular location

Golgi apparatusGolgi stack. Endoplasmic reticulum. Note: Resides predominantly in the cisternal stacks of the Golgi. Colocalizes with TRIM13 on the perinuclear endoplasmic reticulum. Ref.2 Ref.9

Tissue specificity

Isoform 1 is expressed in all tissues examined, including, brain, placenta, lung, liver, skeletal muscle, pancreas and kidney. Isoform 1 and isoform 2 are highly expressed in heart. Isoform 2 is also detected in the testis.

Sequence similarities

Contains 2 FYVE-type zinc fingers.

Sequence caution

The sequence AAL55826.1 differs from that shown. Reason: Frameshift at position 480.

The sequence BAB13415.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB55085.1 differs from that shown. Reason: Erroneous termination at position 760. Translated as Trp.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PPP1CAP621362EBI-4401611,EBI-357253

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9HBF4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9HBF4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-415: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 777777Zinc finger FYVE domain-containing protein 1
PRO_0000098713

Regions

Zinc finger598 – 65962FYVE-type 1
Zinc finger715 – 77561FYVE-type 2

Natural variations

Alternative sequence1 – 415415Missing in isoform 2.
VSP_008007

Experimental info

Mutagenesis6161T → R: Partially restore PtdIns3P binding; when associated with R-733. Ref.3
Mutagenesis6171K → A: Drastically reduce PtdIns3P binding; when associated with A-619 and A-621. Abolishes PtdIns3P binding; when associated with A-734; A-736 and A-738. Ref.3
Mutagenesis6191H → A: Drastically reduce PtdIns3P binding; when associated with A-617 and A-621. Abolishes PtdIns3P binding; when associated with A-734; A-736 and A-738. Ref.3
Mutagenesis6211R → A: Drastically reduce PtdIns3P binding; when associated with A-617 and A-619. Abolishes PtdIns3P binding; when associated with A-734; A-736 and A-738. Ref.3
Mutagenesis6541C → S: Abolishes PtdIns3P binding; when associated with S-770. Ref.3
Mutagenesis7331S → R: Partially restored PtdIns3P binding; when associated with R-616. Ref.3
Mutagenesis7341K → A: Drastically reduce PtdIns3P binding; when associated with A-736 and A-738. Abolishes PtdIns3P binding; when associated with A-617; A-619 and A-621. Ref.3
Mutagenesis7361H → A: Drastically reduce PtdIns3P binding; when associated with A-734 and A-738. Abolishes PtdIns3P binding; when associated with A-617; A-619 and A-621. Ref.3
Mutagenesis7381R → A: Drastically reduce PtdIns3P binding; when associated with A-734 and A-736. Abolishes PtdIns3P binding; when associated with A-617; A-619 and A-621. Ref.3
Mutagenesis7701C → S: Abolishes PtdIns3P binding; when associated with S-654. Ref.2 Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 11B5E561066CBCD5

FASTA77787,176
        10         20         30         40         50         60 
MSAQTSPAEK GLNPGLMCQE SYACSGTDEA IFECDECCSL QCLRCEEELH RQERLRNHER 

        70         80         90        100        110        120 
IRLKPGHVPY CDLCKGLSGH LPGVRQRAIV RCQTCKINLC LECQKRTHSG GNKRRHPVTV 

       130        140        150        160        170        180 
YNVSNLQESL EAEEMDEETK RKKMTEKVVS FLLVDENEEI QVTNEEDFIR KLDCKPDQHL 

       190        200        210        220        230        240 
KVVSIFGNTG DGKSHTLNHT FFYGREVFKT SPTQESCTVG VWAAYDPVHK VAVIDTEGLL 

       250        260        270        280        290        300 
GATVNLSQRT RLLLKVLAIS DLVIYRTHAD RLHNDLFKFL GDASEAYLKH FTKELKATTA 

       310        320        330        340        350        360 
RCGLDVPLST LGPAVIIFHE TVHTQLLGSD HPSEVPEKLI QDRFRKLGRF PEAFSSIHYK 

       370        380        390        400        410        420 
GTRTYNPPTD FSGLRRALEQ LLENNTTRSP RHPGVIFKAL KALSDRFSGE IPDDQMAHSS 

       430        440        450        460        470        480 
FFPDEYFTCS SLCLSCGVGC KKSMNHGKEG VPHEAKSRCR YSHQYDNRVY TCKACYERGE 

       490        500        510        520        530        540 
EVSVVPKTSA STDSPWMGLA KYAWSGYVIE CPNCGVVYRS RQYWFGNQDP VDTVVRTEIV 

       550        560        570        580        590        600 
HVWPGTDGFL KDNNNAAQRL LDGMNFMAQS VSELSLGPTK AVTSWLTDQI APAYWRPNSQ 

       610        620        630        640        650        660 
ILSCNKCATS FKDNDTKHHC RACGEGFCDS CSSKTRPVPE RGWGPAPVRV CDNCYEARNV 

       670        680        690        700        710        720 
QLAVTEAQVD DEGGTLIARK VGEAVQNTLG AVVTAIDIPL GLVKDAARPA YWVPDHEILH 

       730        740        750        760        770 
CHNCRKEFSI KLSKHHCRAC GQGFCDECSH DRRAVPSRGW DHPVRVCFNC NKKPGDL 

« Hide

Isoform 2 [UniParc].

Checksum: 70B405CA16F3EE9D
Show »

FASTA36240,260

References

« Hide 'large scale' references
[1]"Double FYVE-containing protein 1 (DFCP1): isolation, cloning and characterization of a novel FYVE finger protein from a human bone marrow cDNA library."
Derubeis A.R., Young M.F., Jia L., Robey P.G., Fisher L.W.
Gene 255:195-203(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
Tissue: Bone marrow stroma.
[2]"FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments."
Ridley S.H., Ktistakis N., Davidson K., Anderson K.E., Manifava M., Ellson C.D., Lipp P., Bootman M., Coadwell J., Nazarian A., Erdjument-Bromage H., Tempst P., Cooper M.A., Thuring J.W.J.F., Lim Z.-Y., Holmes A.B., Stephens L.R., Hawkins P.T.
J. Cell Sci. 114:3991-4000(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INTERACTION WITH PTDINS3P, MUTAGENESIS OF CYS-770.
[3]"Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi."
Cheung P.C.F., Trinkle-Mulcahy L., Cohen P., Lucocq J.M.
Biochem. J. 355:113-121(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION, INTERACTION WITH PTDINS3P, MUTAGENESIS OF THR-616; LYS-617; HIS-619; ARG-621; CYS-654; SER-733; LYS-734; HIS-736; ARG-738 AND CYS-770.
[4]"Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.
DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Mammary gland.
[6]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye.
[8]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 372-777 (ISOFORM 1).
[9]"TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells."
Tomar D., Singh R., Singh A.K., Pandya C.D., Singh R.
Biochim. Biophys. Acta 1823:316-326(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TRIM13, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF251025 mRNA. Translation: AAG23748.1.
AJ310569 mRNA. Translation: CAC83950.1.
AF311602 mRNA. Translation: AAK27339.1.
AB046809 mRNA. Translation: BAB13415.1. Different initiation.
AK027399 mRNA. Translation: BAB55085.1. Different termination.
AL442663 Genomic DNA. No translation available.
BC053520 mRNA. Translation: AAH53520.1.
AF318319 mRNA. Translation: AAL55826.1. Frameshift.
RefSeqNP_001268664.1. NM_001281735.1.
NP_067083.1. NM_021260.3.
NP_848535.1. NM_178441.2.
UniGeneHs.335106.

3D structure databases

ProteinModelPortalQ9HBF4.
SMRQ9HBF4. Positions 152-285, 604-659, 715-771.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119749. 4 interactions.
IntActQ9HBF4. 2 interactions.
MINTMINT-8247369.
STRING9606.ENSP00000326921.

PTM databases

PhosphoSiteQ9HBF4.

Polymorphism databases

DMDM34098716.

Proteomic databases

PaxDbQ9HBF4.
PRIDEQ9HBF4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000394207; ENSP00000377757; ENSG00000165861. [Q9HBF4-2]
ENST00000555072; ENSP00000452232; ENSG00000165861. [Q9HBF4-2]
ENST00000556143; ENSP00000450742; ENSG00000165861. [Q9HBF4-1]
GeneID53349.
KEGGhsa:53349.
UCSCuc001xnl.3. human. [Q9HBF4-2]
uc001xnm.3. human. [Q9HBF4-1]

Organism-specific databases

CTD53349.
GeneCardsGC14M073436.
HGNCHGNC:13180. ZFYVE1.
HPAHPA002898.
MIM605471. gene.
neXtProtNX_Q9HBF4.
PharmGKBPA37752.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG303950.
HOGENOMHOG000044357.
HOVERGENHBG057679.
InParanoidQ9HBF4.
KOK17603.
PhylomeDBQ9HBF4.
TreeFamTF323237.

Gene expression databases

ArrayExpressQ9HBF4.
BgeeQ9HBF4.
CleanExHS_ZFYVE1.
GenevestigatorQ9HBF4.

Family and domain databases

Gene3D3.30.40.10. 2 hits.
3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01363. FYVE. 2 hits.
[Graphical view]
SMARTSM00064. FYVE. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEPS50178. ZF_FYVE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZFYVE1. human.
GeneWikiZFYVE1.
GenomeRNAi53349.
NextBio56004.
PROQ9HBF4.
SOURCESearch...

Entry information

Entry nameZFYV1_HUMAN
AccessionPrimary (citable) accession number: Q9HBF4
Secondary accession number(s): Q8WYX7 expand/collapse secondary AC list , Q96K57, Q9BXP9, Q9HCI3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM