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Protein

Ras-related GTP-binding protein C

Gene

RRAGC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 75GTPBy similarity8
Nucleotide bindingi116 – 120GTPBy similarity5
Nucleotide bindingi178 – 181GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • cell cycle arrest Source: Reactome
  • cell growth Source: UniProtKB
  • cellular protein localization Source: UniProtKB
  • cellular response to amino acid starvation Source: UniProtKB
  • cellular response to amino acid stimulus Source: UniProtKB
  • macroautophagy Source: Reactome
  • positive regulation of TOR signaling Source: GO_Central
  • regulation of autophagy Source: GO_Central
  • regulation of TORC1 signaling Source: UniProtKB
  • regulation of TOR signaling Source: UniProtKB
  • response to amino acid Source: UniProtKB
  • RNA splicing Source: UniProtKB
  • small GTPase mediated signal transduction Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116954-MONOMER.
ReactomeiR-HSA-1632852. Macroautophagy.
R-HSA-165159. mTOR signalling.
R-HSA-166208. mTORC1-mediated signalling.
R-HSA-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-HSA-5628897. TP53 Regulates Metabolic Genes.
SIGNORiQ9HB90.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related GTP-binding protein C
Short name:
Rag C
Short name:
RagC
Alternative name(s):
GTPase-interacting protein 2
TIB929
Gene namesi
Name:RRAGCImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:19902. RRAGC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • EGO complex Source: GO_Central
  • Gtr1-Gtr2 GTPase complex Source: GO_Central
  • intracellular membrane-bounded organelle Source: HPA
  • lysosome Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Lysosome, Nucleus

Pathology & Biotechi

Involvement in diseasei

RRAGC mutations have been found in a patient with idiopathic dilated cardiomyopathy with ventricular dilation and systolic dysfunction, bilateral cataracts, and mild facial dysmorphisms.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75S → L: Increased RPTOR-binding. 1 Publication1
Mutagenesisi120Q → L: Decreased RPTOR-binding. 1 Publication1

Keywords - Diseasei

Disease mutation

Organism-specific databases

OpenTargetsiENSG00000116954.
PharmGKBiPA134862062.

Polymorphism and mutation databases

DMDMi74752776.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002399512 – 399Ras-related GTP-binding protein CAdd BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei15PhosphoserineCombined sources1
Modified residuei96PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9HB90.
MaxQBiQ9HB90.
PaxDbiQ9HB90.
PeptideAtlasiQ9HB90.
PRIDEiQ9HB90.

PTM databases

iPTMnetiQ9HB90.
PhosphoSitePlusiQ9HB90.

Expressioni

Gene expression databases

BgeeiENSG00000116954.
CleanExiHS_RRAGC.
GenevisibleiQ9HB90. HS.

Organism-specific databases

HPAiHPA018247.
HPA055489.

Interactioni

Subunit structurei

Forms a heterodimer with RRAGA, in a sequence-independent manner, and RRAGB. Heterodimerization stabilizes proteins of the heterodimer. In complex with RRAGA or RRAGB, interacts with RPTOR; this interaction is particularly efficient with GTP-loaded RRAGB and GDP-loaded RRAGC. Interacts with NOL8. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGB, is negatively regulated by amino acids and prevents interaction with RPTOR (PubMed:11073942, PubMed:14660641, PubMed:18497260, PubMed:22575674). The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor (PubMed:25561175, PubMed:25567906). Interacts with SESN1, SESN2 AND SESN3 (PubMed:25259925).7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPTORQ8N1225EBI-752390,EBI-1567928
RRAGAQ7L52318EBI-752390,EBI-752376
RRAGBQ5VZM25EBI-752390,EBI-993049
SLC38A9Q8NBW49EBI-752390,EBI-9978316

GO - Molecular functioni

  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi122074. 24 interactors.
DIPiDIP-37515N.
IntActiQ9HB90. 16 interactors.
MINTiMINT-1451634.
STRINGi9606.ENSP00000362092.

Structurei

Secondary structure

1399
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi63 – 69Combined sources7
Helixi74 – 82Combined sources9
Helixi87 – 92Combined sources6
Beta strandi100 – 104Combined sources5
Beta strandi112 – 116Combined sources5
Helixi130 – 135Combined sources6
Beta strandi138 – 145Combined sources8
Helixi151 – 167Combined sources17
Beta strandi172 – 178Combined sources7
Helixi180 – 182Combined sources3
Helixi185 – 205Combined sources21
Beta strandi213 – 218Combined sources6
Helixi224 – 235Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LLUX-ray1.40A60-237[»]
ProteinModelPortaliQ9HB90.
SMRiQ9HB90.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HB90.

Family & Domainsi

Sequence similaritiesi

Belongs to the GTR/RAG GTP-binding protein family.Curated

Phylogenomic databases

eggNOGiKOG3887. Eukaryota.
ENOG410XQS9. LUCA.
GeneTreeiENSGT00550000074708.
HOGENOMiHOG000203695.
HOVERGENiHBG059482.
InParanoidiQ9HB90.
KOiK16186.
OMAiNCRTFQE.
OrthoDBiEOG091G0AN4.
PhylomeDBiQ9HB90.
TreeFamiTF300659.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006762. Gtr1_RagA.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11259. PTHR11259. 1 hit.
PfamiPF04670. Gtr1_RagA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HB90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLQYGAEET PLAGSYGAAD SFPKDFGYGV EEEEEEAAAA GGGVGAGAGG
60 70 80 90 100
GCGPGGADSS KPRILLMGLR RSGKSSIQKV VFHKMSPNET LFLESTNKIY
110 120 130 140 150
KDDISNSSFV NFQIWDFPGQ MDFFDPTFDY EMIFRGTGAL IYVIDAQDDY
160 170 180 190 200
MEALTRLHIT VSKAYKVNPD MNFEVFIHKV DGLSDDHKIE TQRDIHQRAN
210 220 230 240 250
DDLADAGLEK LHLSFYLTSI YDHSIFEAFS KVVQKLIPQL PTLENLLNIF
260 270 280 290 300
ISNSGIEKAF LFDVVSKIYI ATDSSPVDMQ SYELCCDMID VVIDVSCIYG
310 320 330 340 350
LKEDGSGSAY DKESMAIIKL NNTTVLYLKE VTKFLALVCI LREESFERKG
360 370 380 390
LIDYNFHCFR KAIHEVFEVG VTSHRSCGHQ TSASSLKALT HNGTPRNAI
Length:399
Mass (Da):44,224
Last modified:March 1, 2001 - v1
Checksum:i35B3171253CC69CF
GO

Sequence cautioni

The sequence BAB14548 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206A → S in AAG45221 (PubMed:11145883).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07651175S → Y Found in a patient with idiopathic dilated cardiomyopathy; renders cells partially insensitive to amino acid deprivation and result in activated mTORC1 signaling. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF323609 mRNA. Translation: AAG45221.1.
AF272035 mRNA. Translation: AAG32662.1.
AL139260 Genomic DNA. Translation: CAI23049.1.
BC016668 mRNA. Translation: AAH16668.1.
AK023373 mRNA. Translation: BAB14548.1. Different initiation.
CCDSiCCDS430.1.
RefSeqiNP_001258780.1. NM_001271851.1.
NP_071440.1. NM_022157.3.
UniGeneiHs.532461.

Genome annotation databases

EnsembliENST00000373001; ENSP00000362092; ENSG00000116954.
GeneIDi64121.
KEGGihsa:64121.
UCSCiuc001ccq.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF323609 mRNA. Translation: AAG45221.1.
AF272035 mRNA. Translation: AAG32662.1.
AL139260 Genomic DNA. Translation: CAI23049.1.
BC016668 mRNA. Translation: AAH16668.1.
AK023373 mRNA. Translation: BAB14548.1. Different initiation.
CCDSiCCDS430.1.
RefSeqiNP_001258780.1. NM_001271851.1.
NP_071440.1. NM_022157.3.
UniGeneiHs.532461.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LLUX-ray1.40A60-237[»]
ProteinModelPortaliQ9HB90.
SMRiQ9HB90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122074. 24 interactors.
DIPiDIP-37515N.
IntActiQ9HB90. 16 interactors.
MINTiMINT-1451634.
STRINGi9606.ENSP00000362092.

PTM databases

iPTMnetiQ9HB90.
PhosphoSitePlusiQ9HB90.

Polymorphism and mutation databases

DMDMi74752776.

Proteomic databases

EPDiQ9HB90.
MaxQBiQ9HB90.
PaxDbiQ9HB90.
PeptideAtlasiQ9HB90.
PRIDEiQ9HB90.

Protocols and materials databases

DNASUi64121.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373001; ENSP00000362092; ENSG00000116954.
GeneIDi64121.
KEGGihsa:64121.
UCSCiuc001ccq.4. human.

Organism-specific databases

CTDi64121.
GeneCardsiRRAGC.
HGNCiHGNC:19902. RRAGC.
HPAiHPA018247.
HPA055489.
MIMi608267. gene.
neXtProtiNX_Q9HB90.
OpenTargetsiENSG00000116954.
PharmGKBiPA134862062.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3887. Eukaryota.
ENOG410XQS9. LUCA.
GeneTreeiENSGT00550000074708.
HOGENOMiHOG000203695.
HOVERGENiHBG059482.
InParanoidiQ9HB90.
KOiK16186.
OMAiNCRTFQE.
OrthoDBiEOG091G0AN4.
PhylomeDBiQ9HB90.
TreeFamiTF300659.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116954-MONOMER.
ReactomeiR-HSA-1632852. Macroautophagy.
R-HSA-165159. mTOR signalling.
R-HSA-166208. mTORC1-mediated signalling.
R-HSA-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-HSA-5628897. TP53 Regulates Metabolic Genes.
SIGNORiQ9HB90.

Miscellaneous databases

ChiTaRSiRRAGC. human.
EvolutionaryTraceiQ9HB90.
GeneWikiiRRAGC.
GenomeRNAii64121.
PROiQ9HB90.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116954.
CleanExiHS_RRAGC.
GenevisibleiQ9HB90. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006762. Gtr1_RagA.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11259. PTHR11259. 1 hit.
PfamiPF04670. Gtr1_RagA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAGC_HUMAN
AccessioniPrimary (citable) accession number: Q9HB90
Secondary accession number(s): Q9H202, Q9H8Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.