Q9HB89 (NMUR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuromedin-U receptor 1
Short name=NMU-R1

Alternative name(s):
G-protein coupled receptor 66
G-protein coupled receptor FM-3

Gene names

Name:	NMUR1
Synonyms:	GPR66

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for the neuromedin-U and neuromedin-S neuropeptides By similarity. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in greatest abundance in peripheral organs, particularly in elements of the gastrointestinal and urogenital systems with highest levels in testes. In central nervous system structures express levels are much lower than those seen in peripheral organs. Within the CNS, has been detected in highest abundance in the cerebellum, dorsal root ganglia, hippocampus, and spinal cord. Ref.1 Ref.4
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	It is uncertain whether Met-1 or Met-24 is the initiator.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of phospholipase C activity Inferred from direct assay Ref.1. Source: UniProtKB calcium ion transport Inferred from direct assay Ref.1. Source: UniProtKB calcium-mediated signaling Inferred from direct assay Ref.1. Source: UniProtKB chloride transport Inferred from direct assay Ref.1. Source: UniProtKB inositol phosphate-mediated signaling Inferred from direct assay Ref.1. Source: UniProtKB phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from direct assay Ref.1. Source: UniProtKB smooth muscle contraction Inferred from expression pattern Ref.1. Source: UniProtKB
Cellular_component	integral to plasma membrane Traceable author statement Ref.4. Source: ProtInc
Molecular_function	neuromedin U receptor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Neuromedin-U receptor 1		PRO_0000069906
Regions
	Topological domain	1 – 65	65	Extracellular Potential
	Transmembrane	66 – 86	21	Helical; Name=1; Potential
	Topological domain	87 – 97	11	Cytoplasmic Potential
	Transmembrane	98 – 118	21	Helical; Name=2; Potential
	Topological domain	119 – 138	20	Extracellular Potential
	Transmembrane	139 – 161	23	Helical; Name=3; Potential
	Topological domain	162 – 181	20	Cytoplasmic Potential
	Transmembrane	182 – 202	21	Helical; Name=4; Potential
	Topological domain	203 – 235	33	Extracellular Potential
	Transmembrane	236 – 256	21	Helical; Name=5; Potential
	Topological domain	257 – 294	38	Cytoplasmic Potential
	Transmembrane	295 – 315	21	Helical; Name=6; Potential
	Topological domain	316 – 338	23	Extracellular Potential
	Transmembrane	339 – 359	21	Helical; Name=7; Potential
	Topological domain	360 – 426	67	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Disulfide bond	134 ↔ 219		By similarity
Experimental info
	Sequence conflict	203	1	R → Q in AAH36543. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9HB89 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F8DD781C020F04AA	FASTA	426	47,351
	10 20 30 40 50 60 MTPLCLNCSV LPGDLYPGGA RNPMACNGSA ARGHFDPEDL NLTDEALRLK YLGPQQTELF 70 80 90 100 110 120 MPICATYLLI FVVGAVGNGL TCLVILRHKA MRTPTNYYLF SLAVSDLLVL LVGLPLELYE 130 140 150 160 170 180 MWHNYPFLLG VGGCYFRTLL FEMVCLASVL NVTALSVERY VAVVHPLQAR SMVTRAHVRR 190 200 210 220 230 240 VLGAVWGLAM LCSLPNTSLH GIRQLHVPCR GPVPDSAVCM LVRPRALYNM VVQTTALLFF 250 260 270 280 290 300 CLPMAIMSVL YLLIGLRLRR ERLLLMQEAK GRGSAAARSR YTCRLQQHDR GRRQVTKMLF 310 320 330 340 350 360 VLVVVFGICW APFHADRVMW SVVSQWTDGL HLAFQHVHVI SGIFFYLGSA ANPVLYSLMS 370 380 390 400 410 420 SRFRETFQEA LCLGACCHRL RPRHSSHSLS RMTTGSTLCD VGSLGSWVHP LAGNDGPEAQ QETDPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of two neuromedin U receptors differentially expressed in peripheral tissues and the central nervous system." Raddatz R., Wilson A.E., Artymyshyn R., Bonini J.A., Borowsky B., Boteju L.W., Zhou S., Kouranova E.V., Nagorny R., Guevarra M.S., Dai M., Lerman G.S., Vaysse P.J., Branchek T.A., Gerald C., Forray C., Adham N. J. Biol. Chem. 275:32452-32459(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS and Testis.
[4]	"Cloning and characterization of a human and murine T-cell orphan G-protein-coupled receptor similar to the growth hormone secretagogue and neurotensin receptors." Tan C.P., McKee K.K., Liu Q., Palyha O.C., Feighner S.D., Hreniuk D.L., Smith R.G., Howard A.D. Genomics 52:223-229(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-426, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF272362 mRNA. Translation: AAG24793.1. AC017104 Genomic DNA. Translation: AAY24249.1. BC036543 mRNA. Translation: AAH36543.1. BC051914 mRNA. Translation: AAH51914.1. AF044601, AF044600 Genomic DNA. Translation: AAC02680.1.
IPI	IPI00307219.
RefSeq	NP_006047.3. NM_006056.4.
UniGene	Hs.471619.
3D structure databases
ProteinModelPortal	Q9HB89.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000305877.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9HB89.
Polymorphism databases
DMDM	74761608.
Proteomic databases
PaxDb	Q9HB89.
PRIDE	Q9HB89.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000305141; ENSP00000305877; ENSG00000171596.
GeneID	10316.
KEGG	hsa:10316.
UCSC	uc002vry.4. human.
Organism-specific databases
CTD	10316.
GeneCards	GC02M232387.
HGNC	HGNC:4518. NMUR1.
HPA	HPA027895.
MIM	604153. gene.
neXtProt	NX_Q9HB89.
PharmGKB	PA28910.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG308321.
HOGENOM	HOG000113804.
HOVERGEN	HBG103506.
InParanoid	Q9HB89.
KO	K05052.
OMA	GRRQVTK.
OrthoDB	EOG49S66R.
PhylomeDB	Q9HB89.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	Q9HB89.
CleanEx	HS_NMUR1.
Genevestigator	Q9HB89.
GermOnline	ENSG00000171596. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR005390. NeuromedU_rcpt. IPR005391. NeuromedU_rcpt_1. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01565. NEUROMEDINUR. PR01566. NEUROMEDNU1R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1075178.
GenomeRNAi	10316.
NextBio	39099.
SOURCE	Search...

Entry information

Entry name

NMUR1_HUMAN

Accession

Primary (citable) accession number: Q9HB89
Secondary accession number(s): O43664, Q7LDP6, Q8NE20

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.