Reviewed,
UniProtKB/Swiss-Prot Q9HB71 (CYBP_HUMAN)
Last modified
July 7, 2009.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Calcyclin-binding protein Short name=CacyBP Short name=hCacyBP Alternative name(s): Siah-interacting protein S100A6-binding protein | ||||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 228 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
| Subunit structure | Interacts with protein of the S100 family S100A1, S100A6, S100B, S100P and S100A12 at physiological calcium concentrations By similarity. Component of some large E3 complex at least composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts directly with SIAH1, SIAH2 and SKP1. |
| Subcellular location | Nucleus. Cytoplasm. Note: Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. Ref.10 |
| Post-translational modification | Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations. Ref.10 Ref.11 |
| Sequence similarities | Contains 1 CS domain. Contains 1 SGS domain. |
| Sequence caution | The sequence AAG23817.1 differs from that shown. Reason: Frameshift at position 203. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Cellular component | Cytoplasm Nucleus |
| Coding sequence diversity | Alternative splicing |
| PTM | Acetylation Phosphoprotein |
| Technical term | 3D-structure Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleusInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| EPB41 | P11171 | 1 | EBI-1047302,EBI-1050906 | |
| GH1 | P01241 | 1 | EBI-1047302,EBI-1026046 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9HB71-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9HB71-2) Also known as: SIP-S; S; The sequence of this isoform differs from the canonical sequence as follows: 73-80: VKISNYGW → DGISQISL 81-228: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.7 Ref.8 | |||||||||||||||||||||||||||||||||||||||
| Chain | 2 – 228 | 227 | Calcyclin-binding protein | PRO_0000185389 | ||||||||||||||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||||||||||||||
| Domain | 73 – 167 | 95 | CS | |||||||||||||||||||||||||||||||||||||||
| Domain | 168 – 228 | 61 | SGS | |||||||||||||||||||||||||||||||||||||||
| Region | 2 – 80 | 79 | Interaction with SIAH1 | |||||||||||||||||||||||||||||||||||||||
| Region | 73 – 228 | 156 | Interaction with SKP1 | |||||||||||||||||||||||||||||||||||||||
| Region | 154 – 228 | 75 | Interaction with S100A6 By similarity | |||||||||||||||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||||||||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.7 Ref.8 | |||||||||||||||||||||||||||||||||||||||
| Modified residue | 209 | 1 | Phosphothreonine Ref.11 | |||||||||||||||||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||||||||||||||||||
| Alternative sequence | 73 – 80 | 8 | VKISNYGW → DGISQISL in isoform 2. | VSP_010171 | ||||||||||||||||||||||||||||||||||||||
| Alternative sequence | 81 – 228 | 148 | Missing in isoform 2. | VSP_010172 | ||||||||||||||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||||||||||||||
| Sequence conflict | 44 | 1 | M → V in AAG23817. Ref.4 | |||||||||||||||||||||||||||||||||||||||
| Sequence conflict | 177 | 1 | E → K in AAG23817. Ref.4 | |||||||||||||||||||||||||||||||||||||||
| Sequence conflict | 183 | 1 | T → P in AAG23817. Ref.4 | |||||||||||||||||||||||||||||||||||||||
| Sequence conflict | 226 | 1 | T → P in AAG23817. Ref.4 | |||||||||||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||||||||||
| Helix | 3 – 19 | 17 | ||||||||||||||||||||||||||||||||||||||||
| Helix | 23 – 46 | 24 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 61 – 64 | 4 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 79 – 83 | 5 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 86 – 92 | 7 | ||||||||||||||||||||||||||||||||||||||||
| Turn | 94 – 98 | 5 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 103 – 108 | 6 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 110 – 117 | 8 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 121 – 123 | 3 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 125 – 130 | 6 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 132 – 134 | 3 | ||||||||||||||||||||||||||||||||||||||||
| Turn | 138 – 140 | 3 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 142 – 146 | 5 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 149 – 155 | 7 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 157 – 160 | 4 | ||||||||||||||||||||||||||||||||||||||||
| Beta strand | 164 – 167 | 4 | ||||||||||||||||||||||||||||||||||||||||
| Helix | 168 – 174 | 7 | ||||||||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and expression of human calcyclin binding protein (hCacyBP) gene." Liu W.X., Wu J., Zhao Z., Zhou Y., Peng X.Z., Yuan J.G., Qiang B.Q. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:181-186(2002) [PubMed: 12006993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver. |
| [2] | "Amplification and overexpression of the gene encoding the human calcyclin binding protein on chromosome 1q24-q25 in advanced lung carcinomas." Petersen S., Schluens K., Dietel M., Petersen I. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [3] | Rhodes S. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). |
| [4] | "Human acute promyelocytic leukemia cell line NB4's apoptosis related genes." Yu W.-Q., Sun B.-Z., Chai Y.-B., Zhu F., Liu X.-S., Li Z., Lu F., Yan W., Yang H., Zhao Z.-L. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Promyelocytic leukemia. |
| [5] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Skin. |
| [7] | Bienvenut W.V., Boldt K., von Kriegsheim A.F., Kolch W. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-14; 34-41; 112-118 AND 124-143, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Hepatoma. |
| [8] | Bienvenut W.V., Heiserich L., Gottlieb E. Submitted (MAR-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-19 AND 112-118, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Colon carcinoma. |
| [9] | "Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses." Matsuzawa S., Reed J.C. Mol. Cell 7:915-926(2001) [PubMed: 11389839] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORM 2), SUBUNIT OF A COMPLEX WITH UBE2D1; SIAH1; SKP1; APC AND TBL1X, INTERACTION WITH SIAH1; SIAH2 AND SKP1. |
| [10] | "Translocation and phosphorylation of calcyclin binding protein during retinoic acid-induced neuronal differentiation of neuroblastoma SH-SY5Y cells." Wu J., Tan X., Peng X.Z., Yuan J.G., Qiang B.Q. J. Biochem. Mol. Biol. 36:354-358(2003) [PubMed: 12895292] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION. |
| [11] | "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-209, MASS SPECTROMETRY. |
| [12] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [13] | "Solution structure of the core domain of calcyclin binding protein; SIAH-interacting protein (SIP)." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 63-176. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AF057356 mRNA. Translation: AAC21458.1. AF314752 mRNA. Translation: AAG34170.1. AL035305 mRNA. Translation: CAA22910.1. AF275803 mRNA. Translation: AAG23817.1. Frameshift. Z99127 Genomic DNA. Translation: CAQ52581.1. BC005975 mRNA. Translation: AAH05975.1. BC022352 mRNA. Translation: AAH22352.1. BC078151 mRNA. Translation: AAH78151.1. | |||||||||||||||||||||||||
| IPI | IPI00395627. IPI00410678. | ||||||||||||||||||||||||
| RefSeq | NP_055227.1. | ||||||||||||||||||||||||
| UniGene | Hs.508524 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| IntAct | Q9HB71. 18 interactions. | ||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||
| PhosphoSite | Q9HB71. | ||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||
| PRIDE | Q9HB71. | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENSG00000116161. Homo sapiens. [Contig view] | ||||||||||||||||||||||||
| GeneID | 27101. | ||||||||||||||||||||||||
| UCSC | uc001gkj.1. human. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| GeneCards | GC01P173235. | ||||||||||||||||||||||||
| H-InvDB | HIX0001350. HIX0057113. | ||||||||||||||||||||||||
| HGNC | HGNC:30423. CACYBP. | ||||||||||||||||||||||||
| MIM | 606186. gene. | ||||||||||||||||||||||||
| PharmGKB | PA134894213. | ||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| HOGENOM | Q9HB71. | ||||||||||||||||||||||||
| HOVERGEN | Q9HB71. | ||||||||||||||||||||||||
| OMA | Q9HB71. YAWDQSD. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q9HB71. | ||||||||||||||||||||||||
| Bgee | Q9HB71. | ||||||||||||||||||||||||
| CleanEx | HS_CACYBP. | ||||||||||||||||||||||||
| GermOnline | ENSG00000116161. Homo sapiens. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR017447. CS. IPR007052. CS_domain. IPR007699. SGS. IPR015120. Siah-Interact_N. [Graphical view] | ||||||||||||||||||||||||
| Pfam | PF04969. CS. 1 hit. PF05002. SGS. 1 hit. PF09032. Siah-Interact_N. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| PROSITE | PS51203. CS. 1 hit. PS51048. SGS. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| NextBio | 49757. | ||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | CYBP_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9HB71 Secondary accession number(s): B2ZWH2, O60666 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


