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Protein

Calcyclin-binding protein

Gene

CACYBP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Calcyclin-binding protein
Short name:
CacyBP
Short name:
hCacyBP
Alternative name(s):
S100A6-binding protein
Siah-interacting protein
Gene namesi
Name:CACYBP
Synonyms:S100A6BP, SIP
ORF Names:PNAS-107
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:30423. CACYBP.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.

GO - Cellular componenti

  • beta-catenin destruction complex Source: UniProtKB
  • cell body Source: Ensembl
  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • neuron projection Source: Ensembl
  • nuclear envelope lumen Source: Ensembl
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23 – 26KRVR → AAVA: Abolishes interaction with SIAH1. 1 Publication4
Mutagenesisi64V → N: Abolishes interaction with SIAH1; when associated with N-66. 1 Publication1
Mutagenesisi66P → N: Abolishes interaction with SIAH1; when associated with N-64. 1 Publication1

Organism-specific databases

DisGeNETi27101.
OpenTargetsiENSG00000116161.
PharmGKBiPA134894213.

Polymorphism and mutation databases

BioMutaiCACYBP.
DMDMi46576651.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001853892 – 228Calcyclin-binding proteinAdd BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources2 Publications1
Modified residuei3PhosphoserineCombined sources1
Modified residuei8N6-acetyllysineCombined sources1
Modified residuei19N6-acetyllysineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei85N6-acetyllysineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9HB71.
MaxQBiQ9HB71.
PaxDbiQ9HB71.
PeptideAtlasiQ9HB71.
PRIDEiQ9HB71.
TopDownProteomicsiQ9HB71-1. [Q9HB71-1]

2D gel databases

UCD-2DPAGEQ9HB71.

PTM databases

iPTMnetiQ9HB71.
PhosphoSitePlusiQ9HB71.
SwissPalmiQ9HB71.

Expressioni

Gene expression databases

BgeeiENSG00000116161.
CleanExiHS_CACYBP.
ExpressionAtlasiQ9HB71. baseline and differential.
GenevisibleiQ9HB71. HS.

Organism-specific databases

HPAiHPA025753.
HPA057038.

Interactioni

Subunit structurei

Homodimer. Interacts with proteins of the S100 family S100A1, S100A6, S100B, S100P and S100A12 in a calcium-dependent manner (By similarity). Component of some large E3 complex at least composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts directly with SIAH1, SIAH2 and SKP1.By similarity2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi118001. 57 interactors.
IntActiQ9HB71. 93 interactors.
MINTiMINT-200516.
STRINGi9606.ENSP00000356652.

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 19Combined sources17
Helixi23 – 46Combined sources24
Beta strandi61 – 64Combined sources4
Beta strandi79 – 83Combined sources5
Beta strandi86 – 92Combined sources7
Turni94 – 98Combined sources5
Beta strandi103 – 108Combined sources6
Beta strandi110 – 117Combined sources8
Beta strandi121 – 123Combined sources3
Beta strandi125 – 130Combined sources6
Beta strandi132 – 134Combined sources3
Turni138 – 140Combined sources3
Beta strandi142 – 146Combined sources5
Beta strandi149 – 155Combined sources7
Beta strandi157 – 160Combined sources4
Beta strandi164 – 167Combined sources4
Helixi168 – 174Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X5MNMR-A63-176[»]
2A25X-ray2.20B58-70[»]
2A26X-ray1.20A/B/C1-47[»]
ProteinModelPortaliQ9HB71.
SMRiQ9HB71.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HB71.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 167CSPROSITE-ProRule annotationAdd BLAST95
Domaini168 – 228SGSPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 80Interaction with SIAH1Add BLAST79
Regioni73 – 228Interaction with SKP11 PublicationAdd BLAST156
Regioni154 – 228Interaction with S100A6By similarityAdd BLAST75

Sequence similaritiesi

Contains 1 CS domain.PROSITE-ProRule annotation
Contains 1 SGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3260. Eukaryota.
ENOG410XSB6. LUCA.
GeneTreeiENSGT00390000016470.
HOGENOMiHOG000238284.
HOVERGENiHBG003242.
InParanoidiQ9HB71.
KOiK04507.
OMAiMKKMYND.
OrthoDBiEOG091G0LQC.
PhylomeDBiQ9HB71.
TreeFamiTF323891.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR015120. Siah-Interact_N.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
PF09032. Siah-Interact_N. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9HB71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASEELQKDL EEVKVLLEKA TRKRVRDALT AEKSKIETEI KNKMQQKSQK
60 70 80 90 100
KAELLDNEKP AAVVAPITTG YTVKISNYGW DQSDKFVKIY ITLTGVHQVP
110 120 130 140 150
TENVQVHFTE RSFDLLVKNL NGKSYSMIVN NLLKPISVEG SSKKVKTDTV
160 170 180 190 200
LILCRKKVEN TRWDYLTQVE KECKEKEKPS YDTETDPSEG LMNVLKKIYE
210 220
DGDDDMKRTI NKAWVESREK QAKGDTEF
Length:228
Mass (Da):26,210
Last modified:April 26, 2004 - v2
Checksum:i88C822FB14A7EF89
GO
Isoform 2 (identifier: Q9HB71-2) [UniParc]FASTAAdd to basket
Also known as: SIP-S, S

The sequence of this isoform differs from the canonical sequence as follows:
     73-80: VKISNYGW → DGISQISL
     81-228: Missing.

Show »
Length:80
Mass (Da):8,956
Checksum:i13A2FAC2429FA9FD
GO
Isoform 3 (identifier: Q9HB71-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Note: No experimental confirmation available.
Show »
Length:185
Mass (Da):21,228
Checksum:i99286837B26B2395
GO

Sequence cautioni

The sequence AAG23817 differs from that shown. Reason: Frameshift at position 203.Curated
The sequence BAG52713 differs from that shown. Reason: Frameshift at position 3.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44M → V in AAG23817 (Ref. 4) Curated1
Sequence conflicti177E → K in AAG23817 (Ref. 4) Curated1
Sequence conflicti183T → P in AAG23817 (Ref. 4) Curated1
Sequence conflicti226T → P in AAG23817 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0468621 – 43Missing in isoform 3. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_01017173 – 80VKISNYGW → DGISQISL in isoform 2. Curated8
Alternative sequenceiVSP_01017281 – 228Missing in isoform 2. CuratedAdd BLAST148

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057356 mRNA. Translation: AAC21458.1.
AF314752 mRNA. Translation: AAG34170.1.
AL035305 mRNA. Translation: CAA22910.1.
AF275803 mRNA. Translation: AAG23817.1. Frameshift.
AY423723 mRNA. Translation: AAS00486.1.
AK093425 mRNA. Translation: BAG52713.1. Frameshift.
AK313278 mRNA. Translation: BAG36086.1.
Z99127 Genomic DNA. Translation: CAI18938.1.
Z99127 Genomic DNA. Translation: CAQ52581.1.
CH471067 Genomic DNA. Translation: EAW90982.1.
BC005975 mRNA. Translation: AAH05975.1.
BC022352 mRNA. Translation: AAH22352.1.
BC078151 mRNA. Translation: AAH78151.1.
CCDSiCCDS1315.1. [Q9HB71-1]
CCDS30942.1. [Q9HB71-3]
RefSeqiNP_001007215.1. NM_001007214.1. [Q9HB71-3]
NP_055227.1. NM_014412.2. [Q9HB71-1]
XP_016856535.1. XM_017001046.1. [Q9HB71-3]
UniGeneiHs.508524.

Genome annotation databases

EnsembliENST00000367679; ENSP00000356652; ENSG00000116161. [Q9HB71-1]
ENST00000405362; ENSP00000385771; ENSG00000116161. [Q9HB71-3]
ENST00000406752; ENSP00000384139; ENSG00000116161. [Q9HB71-2]
ENST00000613570; ENSP00000479414; ENSG00000116161. [Q9HB71-3]
GeneIDi27101.
KEGGihsa:27101.
UCSCiuc001gki.2. human. [Q9HB71-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057356 mRNA. Translation: AAC21458.1.
AF314752 mRNA. Translation: AAG34170.1.
AL035305 mRNA. Translation: CAA22910.1.
AF275803 mRNA. Translation: AAG23817.1. Frameshift.
AY423723 mRNA. Translation: AAS00486.1.
AK093425 mRNA. Translation: BAG52713.1. Frameshift.
AK313278 mRNA. Translation: BAG36086.1.
Z99127 Genomic DNA. Translation: CAI18938.1.
Z99127 Genomic DNA. Translation: CAQ52581.1.
CH471067 Genomic DNA. Translation: EAW90982.1.
BC005975 mRNA. Translation: AAH05975.1.
BC022352 mRNA. Translation: AAH22352.1.
BC078151 mRNA. Translation: AAH78151.1.
CCDSiCCDS1315.1. [Q9HB71-1]
CCDS30942.1. [Q9HB71-3]
RefSeqiNP_001007215.1. NM_001007214.1. [Q9HB71-3]
NP_055227.1. NM_014412.2. [Q9HB71-1]
XP_016856535.1. XM_017001046.1. [Q9HB71-3]
UniGeneiHs.508524.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X5MNMR-A63-176[»]
2A25X-ray2.20B58-70[»]
2A26X-ray1.20A/B/C1-47[»]
ProteinModelPortaliQ9HB71.
SMRiQ9HB71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118001. 57 interactors.
IntActiQ9HB71. 93 interactors.
MINTiMINT-200516.
STRINGi9606.ENSP00000356652.

PTM databases

iPTMnetiQ9HB71.
PhosphoSitePlusiQ9HB71.
SwissPalmiQ9HB71.

Polymorphism and mutation databases

BioMutaiCACYBP.
DMDMi46576651.

2D gel databases

UCD-2DPAGEQ9HB71.

Proteomic databases

EPDiQ9HB71.
MaxQBiQ9HB71.
PaxDbiQ9HB71.
PeptideAtlasiQ9HB71.
PRIDEiQ9HB71.
TopDownProteomicsiQ9HB71-1. [Q9HB71-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367679; ENSP00000356652; ENSG00000116161. [Q9HB71-1]
ENST00000405362; ENSP00000385771; ENSG00000116161. [Q9HB71-3]
ENST00000406752; ENSP00000384139; ENSG00000116161. [Q9HB71-2]
ENST00000613570; ENSP00000479414; ENSG00000116161. [Q9HB71-3]
GeneIDi27101.
KEGGihsa:27101.
UCSCiuc001gki.2. human. [Q9HB71-1]

Organism-specific databases

CTDi27101.
DisGeNETi27101.
GeneCardsiCACYBP.
HGNCiHGNC:30423. CACYBP.
HPAiHPA025753.
HPA057038.
MIMi606186. gene.
neXtProtiNX_Q9HB71.
OpenTargetsiENSG00000116161.
PharmGKBiPA134894213.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3260. Eukaryota.
ENOG410XSB6. LUCA.
GeneTreeiENSGT00390000016470.
HOGENOMiHOG000238284.
HOVERGENiHBG003242.
InParanoidiQ9HB71.
KOiK04507.
OMAiMKKMYND.
OrthoDBiEOG091G0LQC.
PhylomeDBiQ9HB71.
TreeFamiTF323891.

Miscellaneous databases

ChiTaRSiCACYBP. human.
EvolutionaryTraceiQ9HB71.
GeneWikiiCACYBP.
GenomeRNAii27101.
PROiQ9HB71.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116161.
CleanExiHS_CACYBP.
ExpressionAtlasiQ9HB71. baseline and differential.
GenevisibleiQ9HB71. HS.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR015120. Siah-Interact_N.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
PF09032. Siah-Interact_N. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYBP_HUMAN
AccessioniPrimary (citable) accession number: Q9HB71
Secondary accession number(s): B2ZWH2
, B3KSF1, O60666, Q5R370, Q5R371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.