Q9HB40 (RISC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinoid-inducible serine carboxypeptidase
EC=3.4.16.-

Alternative name(s):
Serine carboxypeptidase 1

Gene names

Name:	SCPEP1
Synonyms:	RISC, SCP1
ORF Names:	MSTP034, UNQ265/PRO302

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in vascular wall and kidney homeostasis By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the peptidase S10 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Molecular function	Carboxypeptidase Hydrolase Protease
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW retinoic acid metabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9HB40-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9HB40-2)

The sequence of this isoform differs from the canonical sequence as follows:
     295-296: CL → WF
     297-452: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.6
	Chain	27 – 452	426	Retinoid-inducible serine carboxypeptidase		PRO_0000004284
Sites
	Active site	167	1	By similarity
	Active site	371	1	By similarity
	Active site	431	1	By similarity
Amino acid modifications
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	362	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	295 – 296	2	CL → WF in isoform 2.		VSP_008555
	Alternative sequence	297 – 452	156	Missing in isoform 2.		VSP_008556
	Natural variant	3	1	L → V. Corresponds to variant rs34108204 [ dbSNP | Ensembl ].		VAR_048684
	Natural variant	241	1	V → I. Corresponds to variant rs16957938 [ dbSNP | Ensembl ].		VAR_048685

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 40D8FB3CBC09E3DE	FASTA	452	50,831
	10 20 30 40 50 60 MELALRRSPV PRWLLLLPLL LGLNAGAVID WPTEEGKEVW DYVTVRKDAY MFWWLYYATN 70 80 90 100 110 120 SCKNFSELPL VMWLQGGPGG SSTGFGNFEE IGPLDSDLKP RKTTWLQAAS LLFVDNPVGT 130 140 150 160 170 180 GFSYVNGSGA YAKDLAMVAS DMMVLLKTFF SCHKEFQTVP FYIFSESYGG KMAAGIGLEL 190 200 210 220 230 240 YKAIQRGTIK CNFAGVALGD SWISPVDSVL SWGPYLYSMS LLEDKGLAEV SKVAEQVLNA 250 260 270 280 290 300 VNKGLYREAT ELWGKAEMII EQNTDGVNFY NILTKSTPTS TMESSLEFTQ SHLVCLCQRH 310 320 330 340 350 360 VRHLQRDALS QLMNGPIRKK LKIIPEDQSW GGQATNVFVN MEEDFMKPVI SIVDELLEAG 370 380 390 400 410 420 INVTVYNGQL DLIVDTMGQE AWVRKLKWPE LPKFSQLKWK ALYSDPKSLE TSAFVKSYKN 430 440 450 LAFYWILKAG HMVPSDQGDM ALKMMRLVTQ QE
	Isoform 2 [UniParc]. Checksum: 883AD532A098E7D3	FASTA	296	32,957
	10 20 30 40 50 60 MELALRRSPV PRWLLLLPLL LGLNAGAVID WPTEEGKEVW DYVTVRKDAY MFWWLYYATN 70 80 90 100 110 120 SCKNFSELPL VMWLQGGPGG SSTGFGNFEE IGPLDSDLKP RKTTWLQAAS LLFVDNPVGT 130 140 150 160 170 180 GFSYVNGSGA YAKDLAMVAS DMMVLLKTFF SCHKEFQTVP FYIFSESYGG KMAAGIGLEL 190 200 210 220 230 240 YKAIQRGTIK CNFAGVALGD SWISPVDSVL SWGPYLYSMS LLEDKGLAEV SKVAEQVLNA 250 260 270 280 290 VNKGLYREAT ELWGKAEMII EQNTDGVNFY NILTKSTPTS TMESSLEFTQ SHLVWF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of novel serine carboxypeptidase precursor." Cho J.-J., Baik H.-H. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary gland.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Uterus.
[5]	Liu B., Liu Y.Q., Wang X.Y., Zhao B., Sheng H., Zhao X.W., Liu S., Xu Y.Y., Ye J., Song L., Gao Y., Zhang C.L., Zhang J., Wei Y.J., Cao H.Q., Zhao Y., Liu L.S., Ding J.F. , Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-452 (ISOFORM 1). Tissue: Aorta.
[6]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-41.
[7]	"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-126.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF282618 mRNA. Translation: AAG16692.1. AY358559 mRNA. Translation: AAQ88923.1. AK027373 mRNA. Translation: BAB55069.1. BC010078 mRNA. No translation available. BC072405 mRNA. Translation: AAH72405.1. AF113214 mRNA. Translation: AAG39285.1.
IPI	IPI00012426. IPI00477895.
RefSeq	NP_067639.1. NM_021626.2.
UniGene	Hs.514950.
3D structure databases
ProteinModelPortal	Q9HB40.
ModBase	Search...
Protein family/group databases
MEROPS	S10.013.
PTM databases
PhosphoSite	Q9HB40.
Polymorphism databases
DMDM	41690765.
Proteomic databases
PaxDb	Q9HB40.
PRIDE	Q9HB40.
Protocols and materials databases
DNASU	59342.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262288; ENSP00000262288; ENSG00000121064. ENST00000576154; ENSP00000458587; ENSG00000121064.
GeneID	59342.
KEGG	hsa:59342.
UCSC	uc002iuv.4. human.
Organism-specific databases
CTD	59342.
GeneCards	GC17P055055.
HGNC	HGNC:29507. SCPEP1.
HPA	HPA039102.
neXtProt	NX_Q9HB40.
PharmGKB	PA134960711.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2939.
HOGENOM	HOG000242971.
HOVERGEN	HBG002816.
InParanoid	Q9HB40.
KO	K09646.
OMA	DCSWGGQ.
OrthoDB	EOG4DNF4M.
PhylomeDB	Q9HB40.
Gene expression databases
ArrayExpress	Q9HB40.
Bgee	Q9HB40.
CleanEx	HS_SCPEP1.
Genevestigator	Q9HB40.
GermOnline	ENSG00000121064. Homo sapiens.
Family and domain databases
InterPro	IPR001563. Peptidase_S10. IPR018202. Peptidase_S10_AS. [Graphical view]
PANTHER	PTHR11802. PTHR11802. 1 hit.
Pfam	PF00450. Peptidase_S10. 1 hit. [Graphical view]
PRINTS	PR00724. CRBOXYPTASEC.
PROSITE	PS00560. CARBOXYPEPT_SER_HIS. False negative. PS00131. CARBOXYPEPT_SER_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	59342.
NextBio	65234.

Entry information

Entry name

RISC_HUMAN

Accession

Primary (citable) accession number: Q9HB40
Secondary accession number(s): Q96A94, Q9H3F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.