Q9HB20 (PKHA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pleckstrin homology domain-containing family A member 3
Short name=PH domain-containing family A member 3

Alternative name(s):
Phosphatidylinositol-four-phosphate adapter protein 1
Short name=FAPP-1
Short name=Phosphoinositol 4-phosphate adapter protein 1

Gene names

Name:	PLEKHA3
Synonyms:	FAPP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in Golgi to cell surface membrane traffic. Induces membrane tubulation. Binds preferentially to phosphatidylinositol 4-phosphate (PtdIns4P). Ref.6
Subunit structure	Interacts with GTP-bound ARF1. Ref.9
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein Ref.6.
Tissue specificity	Widely expressed.
Domain	The PH domain of FAPPS binds the small GTPase ARF1 and phosphatidylinositol-4-phosphate (PtdIns4P) with high selectivity, and is required for recruitment of FAPPs to the trans-Golgi network (TGN) By similarity.
Sequence similarities	Contains 1 PH domain.
Sequence caution	The sequence BAA90927.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Ligand	Lipid-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	1-phosphatidylinositol binding Inferred from electronic annotation. Source: Compara phospholipid binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 300

300

Pleckstrin homology domain-containing family A member 3

PRO_0000053878

Regions

Domain

1 – 93

93

PH

Experimental info

Sequence conflict

284

1

M → T in AAG15199. Ref.1

Secondary structure

1

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300

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Turn

11 – 13

3

Beta strand

15 – 23

9

Beta strand

26 – 32

7

Helix

33 – 38

6

Beta strand

43 – 45

3

Helix

46 – 48

3

Beta strand

50 – 52

3

Beta strand

58 – 65

8

Turn

66 – 68

3

Beta strand

69 – 74

6

Helix

78 – 89

12

Sequences

	Sequence		Length	Mass (Da)
	Q9HB20 [UniParc]. Last modified March 29, 2004. Version 2. Checksum: 0B6EF45C7D17E82C	FASTA	300	33,861
	10 20 30 40 50 60 MEGVLYKWTN YLTGWQPRWF VLDNGILSYY DSQDDVCKGS KGSIKMAVCE IKVHSADNTR 70 80 90 100 110 120 MELIIPGEQH FYMKAVNAAE RQRWLVALGS SKACLTDTRT KKEKEISETS ESLKTKMSEL 130 140 150 160 170 180 RLYCDLLMQQ VHTIQEFVHH DENHSSPSAE NMNEASSLLS ATCNTFITTL EECVKIANAK 190 200 210 220 230 240 FKPEMFQLHH PDPLVSPVSP SPVQMMKRSV SHPGSCSSER SSHSIKEPVS TLHRLSQRRR 250 260 270 280 290 300 RTYSDTDSCS DIPLEDPDRP VHCSKNTLNG DLASATIPEE SRLMAKKQSE SEDTLPSFSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities." Dowler S.J., Currie R.A., Campbell D.G., Deak M., Kular G., Downes C.P., Alessi D.R. Biochem. J. 351:19-31(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Colon.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P." Godi A., Di Campli A., Konstantakopoulos A., Di Tullio G., Alessi D.R., Kular G.S., Daniele T., Marra P., Lucocq J.M., De Matteis M.A. Nat. Cell Biol. 6:393-404(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Structural basis of wedging the Golgi membrane by FAPP pleckstrin homology domains." Lenoir M., Coskun U., Grzybek M., Cao X., Buschhorn S.B., James J., Simons K., Overduin M. EMBO Rep. 11:279-284(2010) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-100.
[9]	"Molecular basis of phosphatidylinositol 4-phosphate and ARF1 GTPase recognition by the FAPP1 pleckstrin homology (PH) domain." He J., Scott J.L., Heroux A., Roy S., Lenoir M., Overduin M., Stahelin R.V., Kutateladze T.G. J. Biol. Chem. 286:18650-18657(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-99 OF MUTANT SER-94, INTERACTION WITH ARF1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF286162 mRNA. Translation: AAG15199.1.
AK000074 mRNA. Translation: BAA90927.1. Different initiation.
AK314459 mRNA. Translation: BAG37067.1.
AC009948 Genomic DNA. Translation: AAX88884.1.
CH471058 Genomic DNA. Translation: EAX11026.1.
BC044567 mRNA. Translation: AAH44567.1.

IPI

IPI00029474.

RefSeq

NP_061964.3. NM_019091.3.

UniGene

Hs.41086.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KCJ	NMR	-	A	1-100	[»]
3RCP	X-ray	1.90	A	1-99	[»]

ProteinModelPortal

Q9HB20.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000234453.

PTM databases

PhosphoSite

Q9HB20.

Polymorphism databases

DMDM

48474646.

Proteomic databases

PaxDb

Q9HB20.

PRIDE

Q9HB20.

Protocols and materials databases

DNASU

65977.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000234453; ENSP00000234453; ENSG00000116095.

GeneID

65977.

KEGG

hsa:65977.

UCSC

uc002umn.3. human.

Organism-specific databases

CTD

65977.

GeneCards

GC02P179345.

HGNC

HGNC:14338. PLEKHA3.

HPA

HPA034497.

MIM

607774. gene.

neXtProt

NX_Q9HB20.

PharmGKB

PA33403.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG331898.

HOGENOM

HOG000231911.

HOVERGEN

HBG053613.

InParanoid

Q9HB20.

OMA

RPVHCSR.

OrthoDB

EOG405S21.

PhylomeDB

Q9HB20.

Gene expression databases

ArrayExpress

Q9HB20.

Bgee

Q9HB20.

CleanEx

HS_PLEKHA3.

Genevestigator

Q9HB20.

GermOnline

ENSG00000116095. Homo sapiens.

Family and domain databases

Gene3D

2.30.29.30. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]

Pfam

PF00169. PH. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9HB20.

GenomeRNAi

65977.

NextBio

67418.

SOURCE

Search...

Entry information

Entry name

PKHA3_HUMAN

Accession

Primary (citable) accession number: Q9HB20
Secondary accession number(s): Q4ZG69, Q86TQ1, Q9NXT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.