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Protein

Pleckstrin homology domain-containing family A member 2

Gene

PLEKHA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane (By similarity).By similarity

GO - Molecular functioni

  1. fibronectin binding Source: UniProtKB
  2. laminin binding Source: UniProtKB
  3. lipid binding Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of cell-matrix adhesion Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family A member 2
Short name:
PH domain-containing family A member 2
Alternative name(s):
Tandem PH domain-containing protein 2
Short name:
TAPP-2
Gene namesi
Name:PLEKHA2
Synonyms:TAPP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:14336. PLEKHA2.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity. Nucleus By similarity
Note: Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. membrane Source: UniProtKB
  3. nucleus Source: UniProtKB-SubCell
  4. plasma membrane Source: UniProtKB-SubCell
  5. protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33402.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Pleckstrin homology domain-containing family A member 2PRO_0000053876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841Phosphoserine1 Publication
Modified residuei349 – 3491Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9HB19.
PaxDbiQ9HB19.
PRIDEiQ9HB19.

Expressioni

Tissue specificityi

Highly expressed in heart, kidney, spleen and peripheral blood leukocytes. Detected at lower levels in brain, skeletal muscle, colon, thymus, liver, small intestine, placenta and lung.

Gene expression databases

BgeeiQ9HB19.
CleanExiHS_PLEKHA2.
GenevestigatoriQ9HB19.

Organism-specific databases

HPAiHPA004706.

Interactioni

Subunit structurei

Binds MPDZ and PTPN13.By similarity

Protein-protein interaction databases

BioGridi121881. 3 interactions.
MINTiMINT-275130.
STRINGi9606.ENSP00000393860.

Structurei

3D structure databases

ProteinModelPortaliQ9HB19.
SMRiQ9HB19. Positions 1-113, 199-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 113107PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini198 – 298101PH 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG45745.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ9HB19.
PhylomeDBiQ9HB19.
TreeFamiTF329516.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HB19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYVDRQNRI CGFLDIEEHE NSGKFLRRYF ILDTQANCLL WYMDNPQNLA
60 70 80 90 100
MGAGAVGALQ LTYISKVSIA TPKQKPKTPF CFVINALSQR YFLQANDQKD
110 120 130 140 150
MKDWVEALNQ ASKITVPKGG GLPMTTEVLK SLAAPPALEK KPQVAYKTEI
160 170 180 190 200
IGGVVVHTPI SQNGGDGQEG SEPGSHTILR RSQSYIPTSG CRASTGPPLI
210 220 230 240 250
KSGYCVKQGN VRKSWKRRFF ALDDFTICYF KCEQDREPLR TIFLKDVLKT
260 270 280 290 300
HECLVKSGDL LMRDNLFEII TSSRTFYVQA DSPEDMHSWI KEIGAAVQAL
310 320 330 340 350
KCHPRETSFS RSISLTRPGS SSLSSGPNSI LCRGRPPLEE KKALCKAPSV
360 370 380 390 400
ASSWQPWTPV PQAGEKLLPP GDTSEDSLFT PRPGEGAPPG VLPSSRIRHR
410 420
SEPQHPKEKP FMFNLDDENI RTSDV
Length:425
Mass (Da):47,255
Last modified:December 20, 2005 - v2
Checksum:i145BBE9CE9E64B4E
GO

Sequence cautioni

The sequence AAG15201.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2441L → F in AAG15201 (PubMed:11001876).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti186 – 1861I → N.
Corresponds to variant rs59439576 [ dbSNP | Ensembl ].
VAR_061516

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC067817 Genomic DNA. No translation available.
AC108863 Genomic DNA. No translation available.
AF286164 mRNA. Translation: AAG15201.1. Different initiation.
CCDSiCCDS75732.1.
RefSeqiNP_067636.1. NM_021623.1.
UniGeneiHs.369123.

Genome annotation databases

EnsembliENST00000420274; ENSP00000393860; ENSG00000169499.
GeneIDi59339.
KEGGihsa:59339.

Polymorphism databases

DMDMi84028237.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC067817 Genomic DNA. No translation available.
AC108863 Genomic DNA. No translation available.
AF286164 mRNA. Translation: AAG15201.1. Different initiation.
CCDSiCCDS75732.1.
RefSeqiNP_067636.1. NM_021623.1.
UniGeneiHs.369123.

3D structure databases

ProteinModelPortaliQ9HB19.
SMRiQ9HB19. Positions 1-113, 199-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121881. 3 interactions.
MINTiMINT-275130.
STRINGi9606.ENSP00000393860.

Polymorphism databases

DMDMi84028237.

Proteomic databases

MaxQBiQ9HB19.
PaxDbiQ9HB19.
PRIDEiQ9HB19.

Protocols and materials databases

DNASUi59339.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000420274; ENSP00000393860; ENSG00000169499.
GeneIDi59339.
KEGGihsa:59339.

Organism-specific databases

CTDi59339.
GeneCardsiGC08P038776.
H-InvDBHIX0007463.
HGNCiHGNC:14336. PLEKHA2.
HPAiHPA004706.
MIMi607773. gene.
neXtProtiNX_Q9HB19.
PharmGKBiPA33402.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG45745.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ9HB19.
PhylomeDBiQ9HB19.
TreeFamiTF329516.

Miscellaneous databases

ChiTaRSiPLEKHA2. human.
GenomeRNAii59339.
NextBioi65216.
PROiQ9HB19.
SOURCEiSearch...

Gene expression databases

BgeeiQ9HB19.
CleanExiHS_PLEKHA2.
GenevestigatoriQ9HB19.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities."
    Dowler S.J., Currie R.A., Campbell D.G., Deak M., Kular G., Downes C.P., Alessi D.R.
    Biochem. J. 351:19-31(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-304.
  3. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPKHA2_HUMAN
AccessioniPrimary (citable) accession number: Q9HB19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: December 20, 2005
Last modified: January 7, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.