Q9HB19 (PKHA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pleckstrin homology domain-containing family A member 2
Short name=PH domain-containing family A member 2

Alternative name(s):
Tandem PH domain-containing protein 2
Short name=TAPP-2

Gene names

Name:	PLEKHA2
Synonyms:	TAPP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane By similarity.
Subunit structure	Binds MPDZ and PTPN13 By similarity.
Subcellular location	Cytoplasm By similarity. Cell membrane; Peripheral membrane protein By similarity. Nucleus By similarity. Note: Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2 By similarity.
Tissue specificity	Highly expressed in heart, kidney, spleen and peripheral blood leukocytes. Detected at lower levels in brain, skeletal muscle, colon, thymus, liver, small intestine, placenta and lung.
Sequence similarities	Contains 2 PH domains.
Sequence caution	The sequence AAG15201.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of cell-matrix adhesion Inferred from mutant phenotype PubMed 19786618. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay PubMed 19786618. Source: UniProtKB membrane Inferred from direct assay PubMed 19786618. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from direct assay PubMed 19786618. Source: UniProtKB
Molecular_function	1-phosphatidylinositol binding Inferred from electronic annotation. Source: Compara fibronectin binding Inferred from mutant phenotype PubMed 19786618. Source: UniProtKB laminin binding Inferred from mutant phenotype PubMed 19786618. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Pleckstrin homology domain-containing family A member 2		PRO_0000053876
Regions
	Domain	7 – 113	107	PH 1
	Domain	198 – 298	101	PH 2
Amino acid modifications
	Modified residue	182	1	Phosphoserine By similarity
	Modified residue	184	1	Phosphoserine Ref.4
	Modified residue	349	1	Phosphoserine Ref.3
Natural variations
	Natural variant	186	1	I → N. Corresponds to variant rs59439576 [ dbSNP | Ensembl ].		VAR_061516
Experimental info
	Sequence conflict	244	1	L → F in AAG15201. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9HB19 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 145BBE9CE9E64B4E	FASTA	425	47,255
	10 20 30 40 50 60 MPYVDRQNRI CGFLDIEEHE NSGKFLRRYF ILDTQANCLL WYMDNPQNLA MGAGAVGALQ 70 80 90 100 110 120 LTYISKVSIA TPKQKPKTPF CFVINALSQR YFLQANDQKD MKDWVEALNQ ASKITVPKGG 130 140 150 160 170 180 GLPMTTEVLK SLAAPPALEK KPQVAYKTEI IGGVVVHTPI SQNGGDGQEG SEPGSHTILR 190 200 210 220 230 240 RSQSYIPTSG CRASTGPPLI KSGYCVKQGN VRKSWKRRFF ALDDFTICYF KCEQDREPLR 250 260 270 280 290 300 TIFLKDVLKT HECLVKSGDL LMRDNLFEII TSSRTFYVQA DSPEDMHSWI KEIGAAVQAL 310 320 330 340 350 360 KCHPRETSFS RSISLTRPGS SSLSSGPNSI LCRGRPPLEE KKALCKAPSV ASSWQPWTPV 370 380 390 400 410 420 PQAGEKLLPP GDTSEDSLFT PRPGEGAPPG VLPSSRIRHR SEPQHPKEKP FMFNLDDENI RTSDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities." Dowler S.J., Currie R.A., Campbell D.G., Deak M., Kular G., Downes C.P., Alessi D.R. Biochem. J. 351:19-31(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-304.
[3]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC067817 Genomic DNA. No translation available. AC108863 Genomic DNA. No translation available. AF286164 mRNA. Translation: AAG15201.1. Different initiation.
IPI	IPI00455835.
RefSeq	NP_067636.1. NM_021623.1.
UniGene	Hs.369123.
3D structure databases
ProteinModelPortal	Q9HB19.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-275130.
STRING	9606.ENSP00000393860.
Polymorphism databases
DMDM	84028237.
Proteomic databases
PaxDb	Q9HB19.
PRIDE	Q9HB19.
Protocols and materials databases
DNASU	59339.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000420274; ENSP00000393860; ENSG00000169499.
GeneID	59339.
KEGG	hsa:59339.
Organism-specific databases
CTD	59339.
GeneCards	GC08P038776.
H-InvDB	HIX0007463.
HGNC	HGNC:14336. PLEKHA2.
HPA	HPA004706.
MIM	607773. gene.
neXtProt	NX_Q9HB19.
PharmGKB	PA33402.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG45745.
HOGENOM	HOG000116175.
HOVERGEN	HBG053612.
InParanoid	Q9HB19.
OrthoDB	EOG4GHZPJ.
Enzyme and pathway databases
Pathway_Interaction_DB	pi3kcipathway. Class I PI3K signaling events.
Gene expression databases
ArrayExpress	Q9HB19.
Bgee	Q9HB19.
CleanEx	HS_PLEKHA2.
Genevestigator	Q9HB19.
GermOnline	ENSG00000169499. Homo sapiens.
Family and domain databases
Gene3D	2.30.29.30. 2 hits.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF00169. PH. 2 hits. [Graphical view]
SMART	SM00233. PH. 2 hits. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PLEKHA2. human.
GenomeRNAi	59339.
NextBio	65216.
SOURCE	Search...

Entry information

Entry name

PKHA2_HUMAN

Accession

Primary (citable) accession number: Q9HB19

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.