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Q9HAZ1

- CLK4_HUMAN

UniProt

Q9HAZ1 - CLK4_HUMAN

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Protein

Dual specificity protein kinase CLK4

Gene

CLK4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

TG003 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei189 – 1891ATPPROSITE-ProRule annotation
Active sitei286 – 2861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1739ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  3. protein serine/threonine kinase activity Source: UniProtKB
  4. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. protein autophosphorylation Source: Ensembl
  2. regulation of RNA splicing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ9HAZ1.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK4 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 4
Gene namesi
Name:CLK4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:13659. CLK4.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi189 – 1891K → R: Loss of function. 1 Publication

Organism-specific databases

PharmGKBiPA26598.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Dual specificity protein kinase CLK4PRO_0000085873Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei136 – 1361Phosphoserine1 Publication
Modified residuei138 – 1381Phosphoserine4 Publications

Post-translational modificationi

Autophosphorylates on all three types of residues.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9HAZ1.
PaxDbiQ9HAZ1.
PRIDEiQ9HAZ1.

PTM databases

PhosphoSiteiQ9HAZ1.

Expressioni

Tissue specificityi

Expressed in liver, kidney, heart, muscle, brain and endothelial cells.2 Publications

Gene expression databases

BgeeiQ9HAZ1.
CleanExiHS_CLK4.
ExpressionAtlasiQ9HAZ1. baseline and differential.
GenevestigatoriQ9HAZ1.

Organism-specific databases

HPAiHPA043746.
HPA048118.

Interactioni

Subunit structurei

Interacts with UBL5.2 Publications

Protein-protein interaction databases

BioGridi121501. 9 interactions.
IntActiQ9HAZ1. 5 interactions.
STRINGi9606.ENSP00000316948.

Structurei

3D structure databases

ProteinModelPortaliQ9HAZ1.
SMRiQ9HAZ1. Positions 146-480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini159 – 475317Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiQ9HAZ1.
KOiK08823.
OMAiCERYVPR.
OrthoDBiEOG7TBC1V.
PhylomeDBiQ9HAZ1.
TreeFamiTF101041.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HAZ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRHSKRTHCP DWDSRESWGH ESYRGSHKRK RRSHSSTQEN RHCKPHHQFK
60 70 80 90 100
ESDCHYLEAR SLNERDYRDR RYVDEYRNDY CEGYVPRHYH RDIESGYRIH
110 120 130 140 150
CSKSSVRSRR SSPKRKRNRH CSSHQSRSKS HRRKRSRSIE DDEEGHLICQ
160 170 180 190 200
SGDVLRARYE IVDTLGEGAF GKVVECIDHG MDGMHVAVKI VKNVGRYREA
210 220 230 240 250
ARSEIQVLEH LNSTDPNSVF RCVQMLEWFD HHGHVCIVFE LLGLSTYDFI
260 270 280 290 300
KENSFLPFQI DHIRQMAYQI CQSINFLHHN KLTHTDLKPE NILFVKSDYV
310 320 330 340 350
VKYNSKMKRD ERTLKNTDIK VVDFGSATYD DEHHSTLVST RHYRAPEVIL
360 370 380 390 400
ALGWSQPCDV WSIGCILIEY YLGFTVFQTH DSKEHLAMME RILGPIPQHM
410 420 430 440 450
IQKTRKRKYF HHNQLDWDEH SSAGRYVRRR CKPLKEFMLC HDEEHEKLFD
460 470 480
LVRRMLEYDP TQRITLDEAL QHPFFDLLKK K
Length:481
Mass (Da):57,492
Last modified:March 1, 2001 - v1
Checksum:iF402C36835CDA306
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti352 – 3521L → F.1 Publication
Corresponds to variant rs35272416 [ dbSNP | Ensembl ].
VAR_040414
Natural varianti363 – 3631I → V.1 Publication
Corresponds to variant rs55746655 [ dbSNP | Ensembl ].
VAR_040415

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294429 mRNA. Translation: AAG10074.1.
CCDSiCCDS4437.1.
RefSeqiNP_065717.1. NM_020666.2.
UniGeneiHs.406557.

Genome annotation databases

EnsembliENST00000316308; ENSP00000316948; ENSG00000113240.
GeneIDi57396.
KEGGihsa:57396.
UCSCiuc003mjf.1. human.

Polymorphism databases

DMDMi34922132.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294429 mRNA. Translation: AAG10074.1 .
CCDSi CCDS4437.1.
RefSeqi NP_065717.1. NM_020666.2.
UniGenei Hs.406557.

3D structure databases

ProteinModelPortali Q9HAZ1.
SMRi Q9HAZ1. Positions 146-480.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121501. 9 interactions.
IntActi Q9HAZ1. 5 interactions.
STRINGi 9606.ENSP00000316948.

Chemistry

BindingDBi Q9HAZ1.
ChEMBLi CHEMBL4203.
GuidetoPHARMACOLOGYi 1993.

PTM databases

PhosphoSitei Q9HAZ1.

Polymorphism databases

DMDMi 34922132.

Proteomic databases

MaxQBi Q9HAZ1.
PaxDbi Q9HAZ1.
PRIDEi Q9HAZ1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000316308 ; ENSP00000316948 ; ENSG00000113240 .
GeneIDi 57396.
KEGGi hsa:57396.
UCSCi uc003mjf.1. human.

Organism-specific databases

CTDi 57396.
GeneCardsi GC05M178029.
HGNCi HGNC:13659. CLK4.
HPAi HPA043746.
HPA048118.
MIMi 607969. gene.
neXtProti NX_Q9HAZ1.
PharmGKBi PA26598.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00580000081366.
HOGENOMi HOG000203417.
HOVERGENi HBG107720.
InParanoidi Q9HAZ1.
KOi K08823.
OMAi CERYVPR.
OrthoDBi EOG7TBC1V.
PhylomeDBi Q9HAZ1.
TreeFami TF101041.

Enzyme and pathway databases

SignaLinki Q9HAZ1.

Miscellaneous databases

ChiTaRSi CLK4. human.
GenomeRNAii 57396.
NextBioi 63494.
PROi Q9HAZ1.
SOURCEi Search...

Gene expression databases

Bgeei Q9HAZ1.
CleanExi HS_CLK4.
ExpressionAtlasi Q9HAZ1. baseline and differential.
Genevestigatori Q9HAZ1.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a cDNA encoding functional human CLK4 kinase and localization to chromosome 5q35."
    Schultz J., Jones T., Bork P., Sheer D., Blencke S., Steyrer S., Wellbrock U., Bevec D., Ullrich A., Wallasch C.
    Genomics 71:368-370(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-189.
    Tissue: Kidney.
  2. "Structural analysis of UBL5, a novel ubiquitin-like modifier."
    McNally T., Huang Q., Janis R.S., Liu Z., Olejniczak E.T., Reilly R.M.
    Protein Sci. 12:1562-1566(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBL5.
  3. Cited for: INTERACTION WITH UBL5.
  4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136 AND SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells."
    Eisenreich A., Bogdanov V.Y., Zakrzewicz A., Pries A., Antoniak S., Poller W., Schultheiss H.P., Rauch U.
    Circ. Res. 104:589-599(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] PHE-352 AND VAL-363.

Entry informationi

Entry nameiCLK4_HUMAN
AccessioniPrimary (citable) accession number: Q9HAZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3