Reviewed,
UniProtKB/Swiss-Prot Q9HAZ1 (CLK4_HUMAN)
Last modified
November 3, 2009.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dual specificity protein kinase CLK4 EC=2.7.12.1 Alternative name(s): CDC-like kinase 4 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 481 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. Required for the regulation of alternative splicing of MAPT/TAU. Ref.1 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | |
| Subcellular location | Nucleus By similarity. |
| Tissue specificity | Expressed in liver, kidney, heart, muscle and brain. Ref.1 |
| Post-translational modification | Autophosphorylates on all three types of residues. |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Nucleus |
| Coding sequence diversity | Polymorphism |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 481 | 481 | Dual specificity protein kinase CLK4 | PRO_0000085873 | |||||
Regions | |||||||||
| Domain | 159 – 475 | 317 | Protein kinase | ||||||
| Nucleotide binding | 165 – 173 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 286 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 189 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 136 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
| Modified residue | 138 | 1 | Phosphoserine Ref.6 Ref.7 Ref.5 | ||||||
Natural variations | |||||||||
| Natural variant | 352 | 1 | L → F | VAR_040414 | |||||
| Natural variant | 363 | 1 | I → V | VAR_040415 | |||||
Experimental info | |||||||||
| Mutagenesis | 189 | 1 | K → R: Loss of function. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular characterization of a cDNA encoding functional human CLK4 kinase and localization to chromosome 5q35." Schultz J., Jones T., Bork P., Sheer D., Blencke S., Steyrer S., Wellbrock U., Bevec D., Ullrich A., Wallasch C. Genomics 71:368-370(2001) [PubMed: 11170754] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-189. Tissue: Kidney. |
| [2] | Erratum Schultz J., Jones T., Bork P., Sheer D., Blencke S., Steyrer S., Wellbrock U., Bevec D., Ullrich A., Wallasch C. Genomics 74:251-251(2001) |
| [3] | "Structural analysis of UBL5, a novel ubiquitin-like modifier." McNally T., Huang Q., Janis R.S., Liu Z., Olejniczak E.T., Reilly R.M. Protein Sci. 12:1562-1566(2003) [PubMed: 12824502] [Abstract] Cited for: INTERACTION WITH UBL5. |
| [4] | "Beacon interacts with cdc2/cdc28-like kinases." Kantham L., Kerr-Bayles L., Godde N., Quick M., Webb R., Sunderland T., Bond J., Walder K., Augert G., Collier G. Biochem. Biophys. Res. Commun. 304:125-129(2003) [PubMed: 12705895] [Abstract] Cited for: INTERACTION WITH UBL5. |
| [5] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136 AND SER-138, MASS SPECTROMETRY. |
| [7] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136 AND SER-138, MASS SPECTROMETRY. |
| [8] | "Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R.Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] PHE-352 AND VAL-363. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AF294429 mRNA. Translation: AAG10074.1. | |
| IPI | IPI00180143. |
| RefSeq | NP_065717.1. |
| UniGene | Hs.406557 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BI8 based on UniProtKB Q00534. |
| SMR | Q9HAZ1. Positions 146-480. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9HAZ1. 1 interaction. |
| STRING | Q9HAZ1. |
PTM databases | |
| PhosphoSite | Q9HAZ1. |
Proteomic databases | |
| PRIDE | Q9HAZ1. |
Genome annotation databases | |
| Ensembl | ENST00000316308; ENSP00000316948; ENSG00000113240; Homo sapiens. [Genome view] |
| GeneID | 57396. |
| KEGG | hsa:57396. |
| UCSC | uc003mjf.1. human. |
Organism-specific databases | |
| CTD | 57396. |
| GeneCards | GC05M177962. |
| H-InvDB | HIX0024806. |
| HGNC | HGNC:13659. CLK4. |
| MIM | 607969. gene. |
| PharmGKB | PA26598. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9HAZ1. |
| HOVERGEN | Q9HAZ1. |
| OMA | PVKRITL. |
Enzyme and pathway databases | |
| BRENDA | 2.7.12.1. 247. |
Gene expression databases | |
| ArrayExpress | Q9HAZ1. |
| Bgee | Q9HAZ1. |
| CleanEx | HS_CLK4. |
| Genevestigator | Q9HAZ1. |
| GermOnline | ENSG00000113240. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 63494. |
| SOURCE | Search... |
Entry information
| Entry name | CLK4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9HAZ1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


