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Protein

Dual specificity protein kinase CLK4

Gene

CLK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

TG003 inhibits its kinase activity and affects the regulation of alternative splicing mediated by phosphorylation of SR proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189ATP1
Active sitei286Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 173ATP9

GO - Molecular functioni

GO - Biological processi

  • protein autophosphorylation Source: Ensembl
  • regulation of RNA splicing Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ9HAZ1.
SIGNORiQ9HAZ1.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein kinase CLK4 (EC:2.7.12.1)
Alternative name(s):
CDC-like kinase 4
Gene namesi
Name:CLK4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113240.12.
HGNCiHGNC:13659. CLK4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189K → R: Loss of function. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000113240.
PharmGKBiPA26598.

Chemistry databases

ChEMBLiCHEMBL4203.
GuidetoPHARMACOLOGYi1993.

Polymorphism and mutation databases

BioMutaiCLK4.
DMDMi34922132.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858731 – 481Dual specificity protein kinase CLK4Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylates on all three types of residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9HAZ1.
MaxQBiQ9HAZ1.
PaxDbiQ9HAZ1.
PeptideAtlasiQ9HAZ1.
PRIDEiQ9HAZ1.

PTM databases

iPTMnetiQ9HAZ1.
PhosphoSitePlusiQ9HAZ1.

Expressioni

Tissue specificityi

Expressed in liver, kidney, heart, muscle, brain and endothelial cells.2 Publications

Gene expression databases

BgeeiENSG00000113240.
CleanExiHS_CLK4.
ExpressionAtlasiQ9HAZ1. baseline and differential.
GenevisibleiQ9HAZ1. HS.

Organism-specific databases

HPAiHPA043746.

Interactioni

Subunit structurei

Interacts with UBL5.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121501. 19 interactors.
IntActiQ9HAZ1. 13 interactors.
STRINGi9606.ENSP00000316948.

Chemistry databases

BindingDBiQ9HAZ1.

Structurei

3D structure databases

ProteinModelPortaliQ9HAZ1.
SMRiQ9HAZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 475Protein kinaseAdd BLAST317

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0671. Eukaryota.
ENOG410XQF2. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000203417.
HOVERGENiHBG107720.
InParanoidiQ9HAZ1.
KOiK08823.
OMAiHSCDVWS.
OrthoDBiEOG091G033A.
PhylomeDBiQ9HAZ1.
TreeFamiTF101041.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HAZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHSKRTHCP DWDSRESWGH ESYRGSHKRK RRSHSSTQEN RHCKPHHQFK
60 70 80 90 100
ESDCHYLEAR SLNERDYRDR RYVDEYRNDY CEGYVPRHYH RDIESGYRIH
110 120 130 140 150
CSKSSVRSRR SSPKRKRNRH CSSHQSRSKS HRRKRSRSIE DDEEGHLICQ
160 170 180 190 200
SGDVLRARYE IVDTLGEGAF GKVVECIDHG MDGMHVAVKI VKNVGRYREA
210 220 230 240 250
ARSEIQVLEH LNSTDPNSVF RCVQMLEWFD HHGHVCIVFE LLGLSTYDFI
260 270 280 290 300
KENSFLPFQI DHIRQMAYQI CQSINFLHHN KLTHTDLKPE NILFVKSDYV
310 320 330 340 350
VKYNSKMKRD ERTLKNTDIK VVDFGSATYD DEHHSTLVST RHYRAPEVIL
360 370 380 390 400
ALGWSQPCDV WSIGCILIEY YLGFTVFQTH DSKEHLAMME RILGPIPQHM
410 420 430 440 450
IQKTRKRKYF HHNQLDWDEH SSAGRYVRRR CKPLKEFMLC HDEEHEKLFD
460 470 480
LVRRMLEYDP TQRITLDEAL QHPFFDLLKK K
Length:481
Mass (Da):57,492
Last modified:March 1, 2001 - v1
Checksum:iF402C36835CDA306
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040414352L → F1 PublicationCorresponds to variant dbSNP:rs35272416Ensembl.1
Natural variantiVAR_040415363I → V1 PublicationCorresponds to variant dbSNP:rs55746655Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF294429 mRNA. Translation: AAG10074.1.
CCDSiCCDS4437.1.
RefSeqiNP_065717.1. NM_020666.2.
UniGeneiHs.406557.

Genome annotation databases

EnsembliENST00000316308; ENSP00000316948; ENSG00000113240.
GeneIDi57396.
KEGGihsa:57396.
UCSCiuc003mjf.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCLK4_HUMAN
AccessioniPrimary (citable) accession number: Q9HAZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2001
Last modified: November 22, 2017
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families