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Protein

Melanoma-associated antigen F1

Gene

MAGEF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.1 Publication

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen F1
Alternative name(s):
MAGE-F1 antigen
Gene namesi
Name:MAGEF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29639. MAGEF1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134920978.

Polymorphism and mutation databases

BioMutaiMAGEF1.
DMDMi300669649.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Melanoma-associated antigen F1PRO_0000156732Add
BLAST

Proteomic databases

EPDiQ9HAY2.
PaxDbiQ9HAY2.
PeptideAtlasiQ9HAY2.
PRIDEiQ9HAY2.

PTM databases

iPTMnetiQ9HAY2.
PhosphoSiteiQ9HAY2.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiQ9HAY2.
CleanExiHS_MAGEF1.
GenevisibleiQ9HAY2. HS.

Organism-specific databases

HPAiHPA046899.

Interactioni

Subunit structurei

Interacts with LNX1, TRIM27 and NSMCE1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MDFIQ997503EBI-5525855,EBI-724076

Protein-protein interaction databases

BioGridi122067. 11 interactions.
IntActiQ9HAY2. 6 interactions.
STRINGi9606.ENSP00000315064.

Structurei

3D structure databases

ProteinModelPortaliQ9HAY2.
SMRiQ9HAY2. Positions 71-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 277202MAGEPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ9HAY2.
OMAiGYPKRLI.
OrthoDBiEOG75F4GM.
PhylomeDBiQ9HAY2.
TreeFamiTF328505.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HAY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQTPESRGL PVPQAEGEKD GGHDGETRAP TASQERPKEE LGAGREEGAA
60 70 80 90 100
EPALTRKGAR ALAAKALARR RAYRRLNRTV AELVQFLLVK DKKKSPITRS
110 120 130 140 150
EMVKYVIGDL KILFPDIIAR AAEHLRYVFG FELKQFDRKH HTYILINKLK
160 170 180 190 200
PLEEEEEEDL GGDGPRLGLL MMILGLIYMR GNSAREAQVW EMLRRLGVQP
210 220 230 240 250
SKYHFLFGYP KRLIMEDFVQ QRYLSYRRVP HTNPPEYEFS WGPRSNLEIS
260 270 280 290 300
KMEVLGFVAK LHKKEPQHWP VQYREALADE ADRARAKARA EASMRARASA

RAGIHLW
Length:307
Mass (Da):35,222
Last modified:July 13, 2010 - v2
Checksum:i8CB673B3FF7921EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661A → S in AAG30208 (PubMed:11313144).Curated
Sequence conflicti66 – 661A → S in AAG38606 (PubMed:16641997).Curated
Sequence conflicti66 – 661A → S in AAH10056 (PubMed:15489334).Curated
Sequence conflicti158 – 1581E → EE in AAG30208 (PubMed:11313144).Curated
Sequence conflicti158 – 1581E → EE in AAG38606 (PubMed:16641997).Curated
Sequence conflicti158 – 1581E → EE in AAH10056 (PubMed:15489334).Curated
Sequence conflicti236 – 2361E → A in AAG30208 (PubMed:11313144).Curated
Sequence conflicti236 – 2361E → A in AAG38606 (PubMed:16641997).Curated
Sequence conflicti236 – 2361E → A in AAH10056 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti93 – 931K → R.
Corresponds to variant rs34540780 [ dbSNP | Ensembl ].
VAR_057651

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295378 mRNA. Translation: AAG30208.1.
AC107294 Genomic DNA. No translation available.
BC010056 mRNA. Translation: AAH10056.1.
AF320910 mRNA. Translation: AAG38606.1.
CCDSiCCDS3269.1.
RefSeqiNP_071432.2. NM_022149.4.
UniGeneiHs.306123.

Genome annotation databases

EnsembliENST00000317897; ENSP00000315064; ENSG00000177383.
GeneIDi64110.
KEGGihsa:64110.
UCSCiuc003fpa.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295378 mRNA. Translation: AAG30208.1.
AC107294 Genomic DNA. No translation available.
BC010056 mRNA. Translation: AAH10056.1.
AF320910 mRNA. Translation: AAG38606.1.
CCDSiCCDS3269.1.
RefSeqiNP_071432.2. NM_022149.4.
UniGeneiHs.306123.

3D structure databases

ProteinModelPortaliQ9HAY2.
SMRiQ9HAY2. Positions 71-285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122067. 11 interactions.
IntActiQ9HAY2. 6 interactions.
STRINGi9606.ENSP00000315064.

PTM databases

iPTMnetiQ9HAY2.
PhosphoSiteiQ9HAY2.

Polymorphism and mutation databases

BioMutaiMAGEF1.
DMDMi300669649.

Proteomic databases

EPDiQ9HAY2.
PaxDbiQ9HAY2.
PeptideAtlasiQ9HAY2.
PRIDEiQ9HAY2.

Protocols and materials databases

DNASUi64110.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000317897; ENSP00000315064; ENSG00000177383.
GeneIDi64110.
KEGGihsa:64110.
UCSCiuc003fpa.4. human.

Organism-specific databases

CTDi64110.
GeneCardsiMAGEF1.
H-InvDBHIX0003923.
HGNCiHGNC:29639. MAGEF1.
HPAiHPA046899.
MIMi609267. gene.
neXtProtiNX_Q9HAY2.
PharmGKBiPA134920978.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000231161.
HOVERGENiHBG006315.
InParanoidiQ9HAY2.
OMAiGYPKRLI.
OrthoDBiEOG75F4GM.
PhylomeDBiQ9HAY2.
TreeFamiTF328505.

Miscellaneous databases

ChiTaRSiMAGEF1. human.
GenomeRNAii64110.
PROiQ9HAY2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9HAY2.
CleanExiHS_MAGEF1.
GenevisibleiQ9HAY2. HS.

Family and domain databases

InterProiIPR002190. MHD_dom.
[Graphical view]
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MAGE-F1, a novel ubiquitously expressed member of the MAGE superfamily."
    Stone B., Schummer M., Paley P.J., Crawford M., Ford M., Urban N., Nelson B.H.
    Gene 267:173-182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "An overview of the MAGE gene family with the identification of all human members of the family."
    Chomez P., De Backer O., Bertrand M., De Plaen E., Boon T., Lucas S.
    Cancer Res. 61:5544-5551(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-283.
    Tissue: Kidney.
  5. "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases."
    Doyle J.M., Gao J., Wang J., Yang M., Potts P.R.
    Mol. Cell 39:963-974(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LNX1; TRIM27 AND NSMCE1.

Entry informationi

Entry nameiMAGF1_HUMAN
AccessioniPrimary (citable) accession number: Q9HAY2
Secondary accession number(s): Q9H215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: July 13, 2010
Last modified: July 6, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.