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Q9HAW8 (UD110_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glucuronosyltransferase 1-10

Short name=UDPGT 1-10
Short name=UGT1*10
Short name=UGT1-10
Short name=UGT1.10
EC=2.4.1.17
Alternative name(s):
UDP-glucuronosyltransferase 1-J
Short name=UGT-1J
Short name=UGT1J
UDP-glucuronosyltransferase 1A10
Gene names
Name:UGT1A10
Synonyms:GNT1, UGT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1. Ref.6

Catalytic activity

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.

Subunit structure

Isoform 1 interacts with isoform 2/i2suggesting that oligomerization is involved in negative regulation of transferase activity by isoform 2. Isoform 1 also interacts with respective i2 isoforms of UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8 and UGT1A9. Ref.7

Subcellular location

Microsome. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.

Tissue specificity

Liver and colon. Isoform 1 and isoform 2 are expressed in colon, esophagus and small intestine; isoform 2 but not isoform 1 is expressed in liver or kidney (Ref.5). Ref.1 Ref.5

Miscellaneous

The gene is part of the UGT1A complex locus which displays alternative use of promoters, first exons and terminal exons. The locus is defined by 13 first exons, which are alternatively spliced to 3 other common exons and 2 alternative terminal exons 5. From the 27 possible mRNA isoforms, 9 produce functionally active polypeptides (UGT1A1, 1A3, 1A4, 1A5, 1A6, 1A7, 1A8, 1A9 and 1A10) called isoforms 1 (i1). Use of an alternative exon 5 (5b) as terminal exon is leading to 9 additional alternatively spliced products termed isoforms i2 and which lack transferase activity.

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9HAW8-1)

Also known as: i1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9HAW8-2)

Also known as: i2; UGT1A10s;

The sequence of this isoform differs from the canonical sequence as follows:
     432-530: SYKENIMRLS...VKKAHKSKTH → RKKQQSGRQM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 530505UDP-glucuronosyltransferase 1-10
PRO_0000036009

Regions

Transmembrane488 – 50417Helical; Potential

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation2921N-linked (GlcNAc...) Potential
Glycosylation3441N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence432 – 53099SYKEN…KSKTH → RKKQQSGRQM in isoform 2.
VSP_053966
Natural variant591M → I. Ref.8
Corresponds to variant rs56935833 [ dbSNP | Ensembl ].
VAR_018354
Natural variant1391E → K.
Corresponds to variant rs10187694 [ dbSNP | Ensembl ].
VAR_052464
Natural variant2021T → I. Ref.8
Corresponds to variant rs58704432 [ dbSNP | Ensembl ].
VAR_018355
Natural variant2441L → I.
Corresponds to variant rs28969685 [ dbSNP | Ensembl ].
VAR_052465

Experimental info

Sequence conflict1 – 2323MARAG…TCGFA → MAPRRVDQPRSFMCVSTADL WLC Ref.1
Sequence conflict401T → A in AAB81537. Ref.1
Sequence conflict1751H → R in AAB81537. Ref.1
Sequence conflict2241F → L in AAB81537. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (i1) [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 73774EEEC7FE37BA

FASTA53059,810
        10         20         30         40         50         60 
MARAGWTSPV PLCVCLLLTC GFAEAGKLLV VPMDGSHWFT MQSVVEKLIL RGHEVVVVMP 

        70         80         90        100        110        120 
EVSWQLERSL NCTVKTYSTS YTLEDQNREF MVFAHAQWKA QAQSIFSLLM SSSSGFLDLF 

       130        140        150        160        170        180 
FSHCRSLFND RKLVEYLKES SFDAVFLDPF DTCGLIVAKY FSLPSVVFTR GIFCHHLEEG 

       190        200        210        220        230        240 
AQCPAPLSYV PNDLLGFSDA MTFKERVWNH IVHLEDHLFC QYLFRNALEI ASEILQTPVT 

       250        260        270        280        290        300 
AYDLYSHTSI WLLRTDFVLD YPKPVMPNMI FIGGINCHQG KPLPMEFEAY INASGEHGIV 

       310        320        330        340        350        360 
VFSLGSMVSE IPEKKAMAIA DALGKIPQTV LWRYTGTRPS NLANNTILVK WLPQNDLLGH 

       370        380        390        400        410        420 
PMTRAFITHA GSHGVYESIC NGVPMVMMPL FGDQMDNAKR METKGAGVTL NVLEMTSEDL 

       430        440        450        460        470        480 
ENALKAVIND KSYKENIMRL SSLHKDRPVE PLDLAVFWVE FVMRHKGAPH LRPAAHDLTW 

       490        500        510        520        530 
YQYHSLDVIG FLLAVVLTVA FITFKCCAYG YRKCLGKKGR VKKAHKSKTH 

« Hide

Isoform 2 (i2) (UGT1A10s) [UniParc].

Checksum: 06D89D99B1C0300E
Show »

FASTA44149,586

References

« Hide 'large scale' references
[1]"Differential expression of the UGT1A locus in human liver, biliary, and gastric tissue: identification of UGT1A7 and UGT1A10 transcripts in extrahepatic tissue."
Strassburg C.P., Oldhafer K., Manns M.P., Tukey R.H.
Mol. Pharmacol. 52:212-220(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"Thirteen UDP-glucuronosyltransferase genes are encoded at the human UGT1 gene complex locus."
Gong Q.H., Cho J.W., Huang T., Potter C., Gholami N., Basu N.K., Kubota S., Carvalho S., Pennington M.W., Owens I.S., Popescu N.C.
Pharmacogenetics 11:357-368(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Colon and Kidney.
[5]"Genetic diversity at the UGT1 locus is amplified by a novel 3' alternative splicing mechanism leading to nine additional UGT1A proteins that act as regulators of glucuronidation activity."
Girard H., Levesque E., Bellemare J., Journault K., Caillier B., Guillemette C.
Pharmacogenet. Genomics 17:1077-1089(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[6]"Structure and concentration changes affect characterization of UGT isoform-specific metabolism of isoflavones."
Tang L., Singh R., Liu Z., Hu M.
Mol. Pharm. 6:1466-1482(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Alternatively spliced products of the UGT1A gene interact with the enzymatically active proteins to inhibit glucuronosyltransferase activity in vitro."
Bellemare J., Rouleau M., Girard H., Harvey M., Guillemette C.
Drug Metab. Dispos. 38:1785-1789(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[8]"Three novel single nucleotide polymorphisms in UGT1A10."
Saeki M., Ozawa S., Saito Y., Jinno H., Hamaguchi T., Nokihara H., Shimada Y., Kunitoh H., Yamamoto N., Ohe Y., Yamada Y., Shirao K., Muto M., Mera K., Goto K., Ohmatsu H., Kubota K., Niho S. expand/collapse author list , Kakinuma R., Minami H., Ohtsu A., Yoshida T., Saijo N., Sawada J.
Drug Metab. Pharmacokinet. 17:488-490(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS ILE-59 AND ILE-202.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U89508 mRNA. Translation: AAB81537.1.
AF297093 Genomic DNA. Translation: AAG30417.1.
AC006985 Genomic DNA. No translation available.
AC019072 Genomic DNA. No translation available.
BC020971 mRNA. Translation: AAH20971.1.
BC053576 mRNA. Translation: AAH53576.1.
BC069210 mRNA. Translation: AAH69210.2.
CCDSCCDS33403.1. [Q9HAW8-1]
PIRJC5656.
RefSeqNP_061948.1. NM_019075.2. [Q9HAW8-1]
UniGeneHs.554822.

3D structure databases

ProteinModelPortalQ9HAW8.
SMRQ9HAW8. Positions 280-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120053. 1 interaction.
STRING9606.ENSP00000343838.

Chemistry

BindingDBQ9HAW8.
ChEMBLCHEMBL1743320.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteQ9HAW8.

Polymorphism databases

DMDM29839636.

Proteomic databases

PaxDbQ9HAW8.
PRIDEQ9HAW8.

Protocols and materials databases

DNASU54575.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000344644; ENSP00000343838; ENSG00000242515. [Q9HAW8-1]
GeneID54575.
KEGGhsa:54575.
UCSCuc002vur.3. human. [Q9HAW8-1]

Organism-specific databases

CTD54575.
GeneCardsGC02P234545.
HGNCHGNC:12531. UGT1A10.
MIM191740. gene.
606435. gene.
neXtProtNX_Q9HAW8.
PharmGKBPA37174.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1819.
HOGENOMHOG000220832.
HOVERGENHBG004033.
KOK00699.
OMACHYLEDA.
OrthoDBEOG7353X5.
PhylomeDBQ9HAW8.
TreeFamTF315472.

Enzyme and pathway databases

BRENDA2.4.1.17. 2681.
SABIO-RKQ9HAW8.

Gene expression databases

BgeeQ9HAW8.
GenevestigatorQ9HAW8.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiUGT1A10.
GenomeRNAi54575.
NextBio57074.
PROQ9HAW8.
SOURCESearch...

Entry information

Entry nameUD110_HUMAN
AccessionPrimary (citable) accession number: Q9HAW8
Secondary accession number(s): O00474, Q6NT91, Q7Z6H8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM