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Q9HAW7

- UD17_HUMAN

UniProt

Q9HAW7 - UD17_HUMAN

Protein

UDP-glucuronosyltransferase 1-7

Gene

UGT1A7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (23 Apr 2003)
      Previous versions | rss
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    Functioni

    UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.1 Publication

    Catalytic activityi

    UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.2 Publications

    GO - Molecular functioni

    1. drug binding Source: BHF-UCL
    2. enzyme binding Source: BHF-UCL
    3. enzyme inhibitor activity Source: BHF-UCL
    4. glucuronosyltransferase activity Source: BHF-UCL
    5. protein heterodimerization activity Source: UniProt
    6. protein homodimerization activity Source: UniProt
    7. protein kinase C binding Source: BHF-UCL
    8. retinoic acid binding Source: BHF-UCL

    GO - Biological processi

    1. cellular glucuronidation Source: UniProt
    2. coumarin metabolic process Source: BHF-UCL
    3. drug metabolic process Source: BHF-UCL
    4. excretion Source: BHF-UCL
    5. flavone metabolic process Source: BHF-UCL
    6. flavonoid glucuronidation Source: BHF-UCL
    7. negative regulation of catalytic activity Source: GOC
    8. negative regulation of cellular glucuronidation Source: UniProtKB
    9. negative regulation of fatty acid metabolic process Source: BHF-UCL
    10. retinoic acid metabolic process Source: BHF-UCL
    11. xenobiotic glucuronidation Source: BHF-UCL

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.17. 2681.
    ReactomeiREACT_6784. Glucuronidation.
    SABIO-RKQ9HAW7.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronosyltransferase 1-7 (EC:2.4.1.17)
    Short name:
    UDPGT 1-7
    Short name:
    UGT1*7
    Short name:
    UGT1-07
    Short name:
    UGT1.7
    Alternative name(s):
    UDP-glucuronosyltransferase 1-G
    Short name:
    UGT-1G
    Short name:
    UGT1G
    UDP-glucuronosyltransferase 1A7
    Gene namesi
    Name:UGT1A7
    Synonyms:GNT1, UGT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:12539. UGT1A7.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: BHF-UCL
    2. endoplasmic reticulum membrane Source: BHF-UCL
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37182.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 530505UDP-glucuronosyltransferase 1-7PRO_0000036006Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi292 – 2921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9HAW7.
    PaxDbiQ9HAW7.
    PRIDEiQ9HAW7.

    PTM databases

    PhosphoSiteiQ9HAW7.

    Expressioni

    Tissue specificityi

    Liver and gastric tissue. Isoform 1 and isoform 2 are expressed in esophagus. Neither isoform is expressed in liver, kidney, colon and small intestine (PubMed:18004212).2 Publications

    Gene expression databases

    BgeeiQ9HAW7.
    GenevestigatoriQ9HAW7.

    Interactioni

    Subunit structurei

    Isoform 1 interacts with isoform 2/i2 suggesting that oligomerization is involved in negative regulation of transferase activity by isoform 2. Isoform 1 also interacts with respective i2 isoforms of UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A8, UGT1A9 and UGT1A10.2 Publications

    Protein-protein interaction databases

    IntActiQ9HAW7. 8 interactions.
    STRINGi9606.ENSP00000362525.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HAW7.
    SMRiQ9HAW7. Positions 280-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei488 – 50417HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1819.
    HOGENOMiHOG000220832.
    HOVERGENiHBG004033.
    KOiK00699.
    OMAiSIAVHRY.
    OrthoDBiEOG7GBFWS.
    PhylomeDBiQ9HAW7.
    TreeFamiTF315472.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9HAW7-1) [UniParc]FASTAAdd to Basket

    Also known as: i1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MARAGWTGLL PLYVCLLLTC GFAKAGKLLV VPMDGSHWFT MQSVVEKLIL    50
    RGHEVVVVMP EVSWQLGRSL NCTVKTYSTS YTLEDQDREF MVFADARWTA 100
    PLRSAFSLLT SSSNGIFDLF FSNCRSLFND RKLVEYLKES CFDAVFLDPF 150
    DACGLIVAKY FSLPSVVFAR GIFCHYLEEG AQCPAPLSYV PRLLLGFSDA 200
    MTFKERVWNH IMHLEEHLFC PYFFKNVLEI ASEILQTPVT AYDLYSHTSI 250
    WLLRTDFVLE YPKPVMPNMI FIGGINCHQG KPVPMEFEAY INASGEHGIV 300
    VFSLGSMVSE IPEKKAMAIA DALGKIPQTV LWRYTGTRPS NLANNTILVK 350
    WLPQNDLLGH PMTRAFITHA GSHGVYESIC NGVPMVMMPL FGDQMDNAKR 400
    METKGAGVTL NVLEMTSEDL ENALKAVIND KSYKENIMRL SSLHKDRPVE 450
    PLDLAVFWVE FVMRHKGAPH LRPAAHDLTW YQYHSLDVIG FLLAVVLTVA 500
    FITFKCCAYG YRKCLGKKGR VKKAHKSKTH 530
    Length:530
    Mass (Da):59,819
    Last modified:April 23, 2003 - v2
    Checksum:i7D759A382AE871D3
    GO
    Isoform 2 (identifier: Q9HAW7-2) [UniParc]FASTAAdd to Basket

    Also known as: i2, UGT1A7s

    The sequence of this isoform differs from the canonical sequence as follows:
         432-530: SYKENIMRLS...VKKAHKSKTH → RKKQQSGRQM

    Show »
    Length:441
    Mass (Da):49,596
    Checksum:i95FB1E90EB501A8F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti412 – 4121V → A in AAB81536. (PubMed:9271343)Curated
    Sequence conflicti433 – 4331Y → F in AAB81536. (PubMed:9271343)Curated

    Polymorphismi

    There are four common allelic UGT1A7 variants which exhibit significant differences in catalytic activity towards 3-, 7-, and 9-hydroxy-benzo(a)pyrene. UGT1A7*3 exhibits a 5.8-fold lower relative Vmax compared to UGT1A7*1, whereas UGT1A7*2 and UGT1A7*4 have a 2.6- and 2.8-fold lower relative Vmax than UGT1A7*1, respectively, suggesting that these mutations confer slow glucuronidation phenotype.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti129 – 1291N → K in allele UGT1A7*2 and allele UGT1A7*3. 4 Publications
    Corresponds to variant rs17868323 [ dbSNP | Ensembl ].
    VAR_015556
    Natural varianti131 – 1311R → K in allele UGT1A7*2 and allele UGT1A7*3. 4 Publications
    VAR_015557
    Natural varianti131 – 1311R → Q.
    Corresponds to variant rs17868324 [ dbSNP | Ensembl ].
    VAR_052462
    Natural varianti208 – 2081W → R in allele UGT1A7*3 and allele UGT1A7*4. 3 Publications
    Corresponds to variant rs11692021 [ dbSNP | Ensembl ].
    VAR_015558

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei432 – 53099SYKEN…KSKTH → RKKQQSGRQM in isoform 2. 1 PublicationVSP_053963Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89507 mRNA. Translation: AAB81536.1.
    AF297093 Genomic DNA. Translation: AAG30419.1.
    DQ383513 mRNA. Translation: ABD42926.1.
    AC006985 Genomic DNA. No translation available.
    AC114812 Genomic DNA. No translation available.
    CCDSiCCDS2506.1. [Q9HAW7-1]
    RefSeqiNP_061950.2. NM_019077.2. [Q9HAW7-1]
    UniGeneiHs.554822.

    Genome annotation databases

    EnsembliENST00000373426; ENSP00000362525; ENSG00000244122. [Q9HAW7-1]
    GeneIDi54577.
    KEGGihsa:54577.
    UCSCiuc002vut.3. human. [Q9HAW7-1]

    Polymorphism databases

    DMDMi30173486.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89507 mRNA. Translation: AAB81536.1 .
    AF297093 Genomic DNA. Translation: AAG30419.1 .
    DQ383513 mRNA. Translation: ABD42926.1 .
    AC006985 Genomic DNA. No translation available.
    AC114812 Genomic DNA. No translation available.
    CCDSi CCDS2506.1. [Q9HAW7-1 ]
    RefSeqi NP_061950.2. NM_019077.2. [Q9HAW7-1 ]
    UniGenei Hs.554822.

    3D structure databases

    ProteinModelPortali Q9HAW7.
    SMRi Q9HAW7. Positions 280-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9HAW7. 8 interactions.
    STRINGi 9606.ENSP00000362525.

    Chemistry

    BindingDBi Q9HAW7.
    ChEMBLi CHEMBL1743317.

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    PTM databases

    PhosphoSitei Q9HAW7.

    Polymorphism databases

    DMDMi 30173486.

    Proteomic databases

    MaxQBi Q9HAW7.
    PaxDbi Q9HAW7.
    PRIDEi Q9HAW7.

    Protocols and materials databases

    DNASUi 54577.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000373426 ; ENSP00000362525 ; ENSG00000244122 . [Q9HAW7-1 ]
    GeneIDi 54577.
    KEGGi hsa:54577.
    UCSCi uc002vut.3. human. [Q9HAW7-1 ]

    Organism-specific databases

    CTDi 54577.
    GeneCardsi GC02P234581.
    HGNCi HGNC:12539. UGT1A7.
    MIMi 191740. gene.
    606432. gene.
    neXtProti NX_Q9HAW7.
    PharmGKBi PA37182.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1819.
    HOGENOMi HOG000220832.
    HOVERGENi HBG004033.
    KOi K00699.
    OMAi SIAVHRY.
    OrthoDBi EOG7GBFWS.
    PhylomeDBi Q9HAW7.
    TreeFami TF315472.

    Enzyme and pathway databases

    BRENDAi 2.4.1.17. 2681.
    Reactomei REACT_6784. Glucuronidation.
    SABIO-RK Q9HAW7.

    Miscellaneous databases

    GeneWikii UGT1A7_(gene).
    GenomeRNAii 54577.
    NextBioi 35481515.
    PROi Q9HAW7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9HAW7.
    Genevestigatori Q9HAW7.

    Family and domain databases

    InterProi IPR002213. UDP_glucos_trans.
    [Graphical view ]
    PANTHERi PTHR11926. PTHR11926. 1 hit.
    Pfami PF00201. UDPGT. 1 hit.
    [Graphical view ]
    PROSITEi PS00375. UDPGT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of the UGT1A locus in human liver, biliary, and gastric tissue: identification of UGT1A7 and UGT1A10 transcripts in extrahepatic tissue."
      Strassburg C.P., Oldhafer K., Manns M.P., Tukey R.H.
      Mol. Pharmacol. 52:212-220(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "Thirteen UDP-glucuronosyltransferase genes are encoded at the human UGT1 gene complex locus."
      Gong Q.H., Cho J.W., Huang T., Potter C., Gholami N., Basu N.K., Kubota S., Carvalho S., Pennington M.W., Owens I.S., Popescu N.C.
      Pharmacogenetics 11:357-368(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS LYS-129; LYS-131 AND ARG-208.
    3. Guillemette C., Levesque E., Girard H., Bernard O.
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANTS LYS-129 AND LYS-131.
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Genetic diversity at the UGT1 locus is amplified by a novel 3' alternative splicing mechanism leading to nine additional UGT1A proteins that act as regulators of glucuronidation activity."
      Girard H., Levesque E., Bellemare J., Journault K., Caillier B., Guillemette C.
      Pharmacogenet. Genomics 17:1077-1089(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, ALTERNATIVE SPLICING, FUNCTION (ISOFORM 2), TISSUE SPECIFICITY.
    6. "Alternatively spliced products of the UGT1A gene interact with the enzymatically active proteins to inhibit glucuronosyltransferase activity in vitro."
      Bellemare J., Rouleau M., Girard H., Harvey M., Guillemette C.
      Drug Metab. Dispos. 38:1785-1789(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, FUNCTION (ISOFORM 2), SUBUNIT.
    7. "The relative protein abundance of UGT1A alternative splice variants as a key determinant of glucuronidation activity in vitro."
      Rouleau M., Roberge J., Falardeau S.A., Villeneuve L., Guillemette C.
      Drug Metab. Dispos. 41:694-697(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    8. "Structural heterogeneity at the UDP-glucuronosyltransferase 1 locus: functional consequences of three novel missense mutations in the human UGT1A7 gene."
      Guillemette C., Ritter J.K., Auyeung D.J., Kessler F.K., Housman D.E.
      Pharmacogenetics 10:629-644(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS LYS-129; LYS-131 AND ARG-208, CHARACTERIZATION OF ALLELES.
    9. "Analysis of inherited genetic variations at the UGT1 locus in the French-Canadian population."
      Menard V., Girard H., Harvey M., Perusse L., Guillemette C.
      Hum. Mutat. 30:677-687(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS LYS-129; LYS-131 AND ARG-208.

    Entry informationi

    Entry nameiUD17_HUMAN
    AccessioniPrimary (citable) accession number: Q9HAW7
    Secondary accession number(s): B8K293, O00473
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 11, 2003
    Last sequence update: April 23, 2003
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The gene is part of the UGT1A complex locus which displays alternative use of promoters, first exons and terminal exons. The locus is defined by 13 first exons, which are alternatively spliced to 3 other common exons and 2 alternative terminal exons 5. From the 27 possible mRNA isoforms, 9 produce functionally active polypeptides (UGT1A1, 1A3, 1A4, 1A5, 1A6, 1A7, 1A8, 1A9 and 1A10) called isoforms 1 (i1). Use of an alternative exon 5 (5b) as terminal exon is leading to 9 additional alternatively spliced products termed isoforms i2 and which lack transferase activity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3