Reviewed, UniProtKB/Swiss-Prot Q9HAW0 (BRF2_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcription factor IIIB 50 kDa subunit
      Short name=TFIIIB50
      Short name=hTFIIIB50
Alternative name(s):
    B-related factor 2
      Short name=BRF-2
      Short name=hBRFU

Gene names

Name:	BRF2
Synonyms:	BRFU
ORF Names:	PRO1470

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Ref.1 Ref.2 Ref.7
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Component of TFIIIB complex. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent. Interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4. Ref.7 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14
Subcellular location	Nucleus. Ref.14
Induction	Down-regulated by epigallocatechin gallate (EGCG) treatment. Ref.15
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation Inferred from electronic annotation. Source: InterPro
Cellular component	transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription regulator activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABCD3	P28288	1	EBI-1055224,EBI-80992
AIFM1	O95831	1	EBI-1055224,EBI-356440
ATAD3A	Q9NVI7	1	EBI-1055224,EBI-352007
COPG	Q9Y678	1	EBI-1055224,EBI-1049127
DPM1	O60762	1	EBI-1055224,EBI-719526
FANCI	Q9NVI1	1	EBI-1055224,EBI-1013291
GCN1L1	Q92616	1	EBI-1055224,EBI-356221
GEMIN4	P57678	1	EBI-1055224,EBI-356700
HEATR2	Q86Y56	1	EBI-1055224,EBI-719966
IPO4	Q8TEX9	1	EBI-1055224,EBI-395967
MARS	P56192	1	EBI-1055224,EBI-355848
MYO1B	O43795	1	EBI-1055224,EBI-351119
NOC2L	Q9Y3T9	1	EBI-1055224,EBI-751547
NUP93	Q8N1F7	1	EBI-1055224,EBI-1042703
OTUD4	Q01804	1	EBI-1055224,EBI-1054396
PTPLAD1	Q9P035	1	EBI-1055224,EBI-359013
SF3B1	O75533	1	EBI-1055224,EBI-876542
SLC3A2	P08195	1	EBI-1055224,EBI-702356
TMEM33	P57088	1	EBI-1055224,EBI-1048629
TNPO3	Q9Y5L0	1	EBI-1055224,EBI-1042571
UNC45A	Q9H3U1	1	EBI-1055224,EBI-1048763
XPO4	Q9C0E2	1	EBI-1055224,EBI-1044855
XPO6	Q96QU8	1	EBI-1055224,EBI-1022896
XPO7	Q9UIA9	1	EBI-1055224,EBI-286668

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Transcription factor IIIB 50 kDa subunit		PRO_0000337187
Regions
	Repeat	72 – 157	86	1
	Repeat	173 – 249	77	2
	Zinc finger	2 – 36	35	TFIIB-type
Sites
	Metal binding	7	1	Zinc By similarity
	Metal binding	10	1	Zinc By similarity
	Metal binding	28	1	Zinc By similarity
	Metal binding	31	1	Zinc By similarity
Experimental info
	Sequence conflict	153	1	Y → C in BAA91975. Ref.3
	Sequence conflict	376	1	S → F in AAG35669. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9HAW0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 90A39F72DBA14888	FASTA	419	46,533
	10 20 30 40 50 60 MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL REVTYSRSTG 70 80 90 100 110 120 ENEQVSRSQQ RGLRRVRDLC RVLQLPPTFE DTAVAYYQQA YRHSGIRAAR LQKKEVLVGC 130 140 150 160 170 180 CVLITCRQHN WPLTMGAICT LLYADLDVFS STYMQIVKLL GLDVPSLCLA ELVKTYCSSF 190 200 210 220 230 240 KLFQASPSVP AKYVEDKEKM LSRTMQLVEL ANETWLVTGR HPLPVITAAT FLAWQSLQPA 250 260 270 280 290 300 DRLSCSLARF CKLANVDLPY PASSRLQELL AVLLRMAEQL AWLRVLRLDK RSVVKHIGDL 310 320 330 340 350 360 LQHRQSLVRS AFRDGTAEVE TREKEPPGWG QGQGEGEVGN NSLGLPQGKR PASPALLLPP 370 380 390 400 410 CMLKSPKRIC PVPPVSTVTG DENISDSEIE QYLRTPQEVR DFQRAQAARQ AATSVPNPP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters." Schramm L., Pendergrast P.S., Sun Y., Hernandez N. Genes Dev. 14:2650-2663(2000) [PubMed: 11040218] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements." Teichmann M., Wang Z., Roeder R.G. Proc. Natl. Acad. Sci. U.S.A. 97:14200-14205(2000) [PubMed: 11121026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta and Umbilical cord blood.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[6]	"Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs." Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C., Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C., He F. Genome Res. 11:1392-1403(2001) [PubMed: 11483580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 264-419. Tissue: Fetal liver.
[7]	"BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein." Cabart P., Murphy S. J. Biol. Chem. 276:43056-43064(2001) [PubMed: 11564744] [Abstract] Cited for: FUNCTION, INTERACTION WITH TBP.
[8]	"Assembly of human small nuclear RNA gene-specific transcription factor IIIB complex de novo on and off promoter." Cabart P., Murphy S. J. Biol. Chem. 277:26831-26838(2002) [PubMed: 12016223] [Abstract] Cited for: IDENTIFICATION IN THE TFIIIB-ALPHA COMPLEX.
[9]	"Redundant cooperative interactions for assembly of a human U6 transcription initiation complex." Ma B., Hernandez N. Mol. Cell. Biol. 22:8067-8078(2002) [PubMed: 12391172] [Abstract] Cited for: SUBUNIT.
[10]	"The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition." Hinkley C.S., Hirsch H.A., Gu L., LaMere B., Henry R.W. J. Biol. Chem. 278:18649-18657(2003) [PubMed: 12621023] [Abstract] Cited for: INTERACTION WITH SNAPC4, SUBUNIT.
[11]	"A minimal RNA polymerase III transcription system from human cells reveals positive and negative regulatory roles for CK2." Hu P., Wu S., Hernandez N. Mol. Cell 12:699-709(2003) [PubMed: 14527415] [Abstract] Cited for: SUBUNIT.
[12]	"Structure-function analysis of the human TFIIB-related factor II protein reveals an essential role for the C-terminal domain in RNA polymerase III transcription." Saxena A., Ma B., Schramm L., Hernandez N. Mol. Cell. Biol. 25:9406-9418(2005) [PubMed: 16227591] [Abstract] Cited for: SUBUNIT.
[13]	"A role for Yin Yang-1 (YY1) in the assembly of snRNA transcription complexes." Emran F., Florens L., Ma B., Swanson S.K., Washburn M.P., Hernandez N. Gene 377:96-108(2006) [PubMed: 16769183] [Abstract] Cited for: SUBUNIT.
[14]	"Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2." Rollins J., Veras I., Cabarcas S., Willis I., Schramm L. Int. J. Biol. Sci. 3:292-302(2007) [PubMed: 17505538] [Abstract] Cited for: INTERACTION WITH MAF1, SUBCELLULAR LOCATION.
[15]	"The green tea component EGCG inhibits RNA polymerase III transcription." Jacob J., Cabarcas S., Veras I., Zaveri N., Schramm L. Biochem. Biophys. Res. Commun. 360:778-783(2007) [PubMed: 17624304] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF298153 mRNA. Translation: AAG30222.1. AF206673 mRNA. Translation: AAG35669.2. AK001914 mRNA. Translation: BAA91975.1. AK315420 mRNA. Translation: BAG37809.1. CH471080 Genomic DNA. Translation: EAW63351.1. BC010648 mRNA. Translation: AAH10648.1. AF130058 mRNA. Translation: AAG35486.1. Different initiation.
IPI	IPI00301190.
RefSeq	NP_060780.2.
UniGene	Hs.709301
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9HAW0. 57 interactions.
PTM databases
PhosphoSite	Q9HAW0.
Proteomic databases
PRIDE	Q9HAW0.
Genome annotation databases
Ensembl	ENSG00000104221. Homo sapiens. [Contig view]
GeneID	55290.
KEGG	hsa:55290.
UCSC	uc003xkk.1. human.
Organism-specific databases
GeneCards	GC08M037820.
HGNC	HGNC:17298. BRF2.
MIM	607013. gene.
PharmGKB	PA25421.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9HAW0.
OMA	Q9HAW0. CMLKPPK.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription.
Gene expression databases
ArrayExpress	Q9HAW0.
Bgee	Q9HAW0.
CleanEx	HS_BRF2.
Family and domain databases
InterPro	IPR006670. Cyclin. IPR013763. Cyclin_related. IPR000812. TFIIB-related. IPR013137. Znf_TFIIB. [Graphical view]
Gene3D	G3DSA:1.10.472.10. Cyclin_related. 1 hit.
PANTHER	PTHR11618. TFIIB_euk_relate. 1 hit.
Pfam	PF08271. TFIIB_Zn_Ribbon. 1 hit. [Graphical view]
SMART	SM00385. CYCLIN. 1 hit. [Graphical view]
PROSITE	PS00782. TFIIB. False negative. PS51134. ZF_TFIIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	59474.
SOURCE	Search...

Entry information

Entry name

BRF2_HUMAN

Accession

Primary (citable) accession number: Q9HAW0
Secondary accession number(s): B2RD62 , Q9H2Y3, Q9H3B3, Q9NUY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 1, 2001
Last modified:	July 7, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)