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Protein

Aminopeptidase RNPEPL1

Gene

RNPEPL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Broad specificity aminopeptidase which preferentially hydrolyzes an N-terminal methionine, citrulline or glutamine.1 Publication

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by calcium but not affected by chloride ions. Inhibited by amastatin and to a lower extent by bestatin. Weakly inhibited by puromycin.1 Publication

Kineticsi

  1. KM=0.37 mM for Met-AMC1 Publication
  2. KM=0.17 mM for Citrulline-AMC1 Publication
  3. KM=1.72 mM for Ala-AMC1 Publication
  4. KM=0.91 mM for Arg-AMC1 Publication
  5. KM=0.89 mM for Asn-AMC1 Publication
  6. KM=0.41 mM for Gln-AMC1 Publication
  7. KM=0.18 mM for His-AMC1 Publication
  8. KM=1.05 mM for Ile-AMC1 Publication
  9. KM=0.34 mM for Leu-AMC1 Publication
  10. KM=1.72 mM for Lys-AMC1 Publication
  11. KM=0.65 mM for Phe-AMC1 Publication
  12. KM=1.76 mM for Ser-AMC1 Publication
  13. KM=0.12 mM for Trp-AMC1 Publication
  14. KM=0.12 mM for Tyr-AMC1 Publication

    pH dependencei

    Optimum pH is 6.6-8.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi353Zinc; catalyticBy similarity1
    Active sitei354Proton acceptorPROSITE-ProRule annotationBy similarity1
    Metal bindingi357Zinc; catalyticBy similarity1
    Metal bindingi376Zinc; catalyticBy similarity1
    Sitei442Transition state stabilizerBy similarity1

    GO - Molecular functioni

    • metalloaminopeptidase activity Source: UniProtKB
    • peptide binding Source: GO_Central
    • zinc ion binding Source: GO_Central

    GO - Biological processi

    • peptide catabolic process Source: GO_Central
    • proteolysis Source: UniProtKB

    Keywordsi

    Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
    LigandMetal-binding, Zinc

    Protein family/group databases

    MEROPSiM01.022.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase RNPEPL11 Publication (EC:3.4.11.-1 Publication)
    Alternative name(s):
    Arginyl aminopeptidase-like 1Imported
    Methionyl aminopeptidase1 Publication (EC:3.4.11.181 Publication)
    Gene namesi
    Name:RNPEPL11 PublicationImported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:10079. RNPEPL1.

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi57140.
    PharmGKBiPA34452.

    Polymorphism and mutation databases

    BioMutaiRNPEPL1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000950941 – 725Aminopeptidase RNPEPL1Add BLAST725

    Proteomic databases

    EPDiQ9HAU8.
    MaxQBiQ9HAU8.
    PaxDbiQ9HAU8.
    PeptideAtlasiQ9HAU8.
    PRIDEiQ9HAU8.

    PTM databases

    iPTMnetiQ9HAU8.
    PhosphoSitePlusiQ9HAU8.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Expressed at relatively higher levels in heart and skeletal muscle.2 Publications

    Gene expression databases

    BgeeiENSG00000142327.
    CleanExiHS_RNPEPL1.
    ExpressionAtlasiQ9HAU8. baseline and differential.
    GenevisibleiQ9HAU8. HS.

    Organism-specific databases

    HPAiHPA036772.

    Interactioni

    Protein-protein interaction databases

    BioGridi121399. 2 interactors.
    IntActiQ9HAU8. 1 interactor.
    STRINGi9606.ENSP00000270357.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HAU8.
    SMRiQ9HAU8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni326 – 330Substrate bindingBy similarity5

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Phylogenomic databases

    eggNOGiKOG1047. Eukaryota.
    COG0308. LUCA.
    HOGENOMiHOG000293296.
    HOVERGENiHBG001274.
    InParanoidiQ9HAU8.
    KOiK09605.
    OMAiVCNRSFF.
    OrthoDBiEOG091G02UX.
    PhylomeDBiQ9HAU8.
    TreeFamiTF300758.

    Family and domain databases

    CDDicd09599. M1_LTA4H. 1 hit.
    InterProiView protein in InterPro
    IPR016024. ARM-type_fold.
    IPR034015. M1_LTA4H.
    IPR001930. Peptidase_M1.
    IPR015211. Peptidase_M1_C.
    IPR014782. Peptidase_M1_N.
    IPR033525. RNPEPL1.
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PTHR11533:SF237. PTHR11533:SF237. 1 hit.
    PfamiView protein in Pfam
    PF09127. Leuk-A4-hydro_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    PRINTSiPR00756. ALADIPTASE.
    SMARTiView protein in SMART
    SM01263. Leuk-A4-hydro_C. 1 hit.
    SUPFAMiSSF48371. SSF48371. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HAU8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAAQCCCRQA PGAEAAPVRP PPEPPPALDV ASASSAQLFR LRHLQLGLEL
    60 70 80 90 100
    RPEARELAGC LVLELCALRP APRALVLDAH PALRLHSAAF RRAPAAAAET
    110 120 130 140 150
    PCAFAFSAPG PGPAPPPPLP AFPEAPGSEP ACCPLAFRVD PFTDYGSSLT
    160 170 180 190 200
    VTLPPELQAH QPFQVILRYT STDAPAIWWL DPELTYGCAK PFVFTQGHSV
    210 220 230 240 250
    CNRSFFPCFD TPAVKCTYSA VVKAPSGVQV LMSATRSAYM EEEGVFHFHM
    260 270 280 290 300
    EHPVPAYLVA LVAGDLKPAD IGPRSRVWAE PCLLPTATSK LSGAVEQWLS
    310 320 330 340 350
    AAERLYGPYM WGRYDIVFLP PSFPIVAMEN PCLTFIISSI LESDEFLVID
    360 370 380 390 400
    VIHEVAHSWF GNAVTNATWE EMWLSEGLAT YAQRRITTET YGAAFTCLET
    410 420 430 440 450
    AFRLDALHRQ MKLLGEDSPV SKLQVKLEPG VNPSHLMNLF TYEKGYCFVY
    460 470 480 490 500
    YLSQLCGDPQ RFDDFLRAYV EKYKFTSVVA QDLLDSFLSF FPELKEQSVD
    510 520 530 540 550
    CRAGLEFERW LNATGPPLAE PDLSQGSSLT RPVEALFQLW TAEPLDQAAA
    560 570 580 590 600
    SASAIDISKW RTFQTALFLD RLLDGSPLPQ EVVMSLSKCY SSLLDSMNAE
    610 620 630 640 650
    IRIRWLQIVV RNDYYPDLHR VRRFLESQMS RMYTIPLYED LCTGALKSFA
    660 670 680 690 700
    LEVFYQTQGR LHPNLRRAIQ QILSQGLGSS TEPASEPSTE LGKAEADTDS
    710 720
    DAQALLLGDE APSSAISLRD VNVSA
    Length:725
    Mass (Da):80,000
    Last modified:March 15, 2017 - v3
    Checksum:i4D8153BD0320A0C4
    GO

    Sequence cautioni

    The sequence AAG22080 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence AAH67258 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence AAX88943 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence BAA91823 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti609V → E in AAG22080 (PubMed:11017071).Curated1
    Sequence conflicti609V → E in BAA91823 (PubMed:14702039).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_036046478V → M in a colorectal cancer sample; somatic mutation. 1 Publication1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC124862 Genomic DNA. Translation: AAX88943.1. Sequence problems.
    BC017301 mRNA. Translation: AAH17301.2.
    BC067258 mRNA. Translation: AAH67258.2. Different initiation.
    BC082975 mRNA. Translation: AAH82975.1.
    AF300795 mRNA. Translation: AAG22080.1. Different initiation.
    AK001668 mRNA. Translation: BAA91823.1. Different initiation.
    AL512754 mRNA. Translation: CAC21674.1.
    RefSeqiNP_060696.4. NM_018226.4.
    UniGeneiHs.5345.

    Genome annotation databases

    EnsembliENST00000270357; ENSP00000270357; ENSG00000142327.
    GeneIDi57140.
    KEGGihsa:57140.
    UCSCiuc061uih.1. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC124862 Genomic DNA. Translation: AAX88943.1. Sequence problems.
    BC017301 mRNA. Translation: AAH17301.2.
    BC067258 mRNA. Translation: AAH67258.2. Different initiation.
    BC082975 mRNA. Translation: AAH82975.1.
    AF300795 mRNA. Translation: AAG22080.1. Different initiation.
    AK001668 mRNA. Translation: BAA91823.1. Different initiation.
    AL512754 mRNA. Translation: CAC21674.1.
    RefSeqiNP_060696.4. NM_018226.4.
    UniGeneiHs.5345.

    3D structure databases

    ProteinModelPortaliQ9HAU8.
    SMRiQ9HAU8.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi121399. 2 interactors.
    IntActiQ9HAU8. 1 interactor.
    STRINGi9606.ENSP00000270357.

    Protein family/group databases

    MEROPSiM01.022.

    PTM databases

    iPTMnetiQ9HAU8.
    PhosphoSitePlusiQ9HAU8.

    Polymorphism and mutation databases

    BioMutaiRNPEPL1.

    Proteomic databases

    EPDiQ9HAU8.
    MaxQBiQ9HAU8.
    PaxDbiQ9HAU8.
    PeptideAtlasiQ9HAU8.
    PRIDEiQ9HAU8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000270357; ENSP00000270357; ENSG00000142327.
    GeneIDi57140.
    KEGGihsa:57140.
    UCSCiuc061uih.1. human.

    Organism-specific databases

    CTDi57140.
    DisGeNETi57140.
    GeneCardsiRNPEPL1.
    H-InvDBiHIX0200261.
    HGNCiHGNC:10079. RNPEPL1.
    HPAiHPA036772.
    MIMi605287. gene.
    neXtProtiNX_Q9HAU8.
    PharmGKBiPA34452.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG1047. Eukaryota.
    COG0308. LUCA.
    HOGENOMiHOG000293296.
    HOVERGENiHBG001274.
    InParanoidiQ9HAU8.
    KOiK09605.
    OMAiVCNRSFF.
    OrthoDBiEOG091G02UX.
    PhylomeDBiQ9HAU8.
    TreeFamiTF300758.

    Miscellaneous databases

    ChiTaRSiRNPEPL1. human.
    GenomeRNAii57140.
    PROiPR:Q9HAU8.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000142327.
    CleanExiHS_RNPEPL1.
    ExpressionAtlasiQ9HAU8. baseline and differential.
    GenevisibleiQ9HAU8. HS.

    Family and domain databases

    CDDicd09599. M1_LTA4H. 1 hit.
    InterProiView protein in InterPro
    IPR016024. ARM-type_fold.
    IPR034015. M1_LTA4H.
    IPR001930. Peptidase_M1.
    IPR015211. Peptidase_M1_C.
    IPR014782. Peptidase_M1_N.
    IPR033525. RNPEPL1.
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PTHR11533:SF237. PTHR11533:SF237. 1 hit.
    PfamiView protein in Pfam
    PF09127. Leuk-A4-hydro_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    PRINTSiPR00756. ALADIPTASE.
    SMARTiView protein in SMART
    SM01263. Leuk-A4-hydro_C. 1 hit.
    SUPFAMiSSF48371. SSF48371. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRNPL1_HUMAN
    AccessioniPrimary (citable) accession number: Q9HAU8
    Secondary accession number(s): Q5XKC3
    , Q6NX56, Q96AC9, Q9H033, Q9NVD0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
    Last sequence update: March 15, 2017
    Last modified: April 12, 2017
    This is version 144 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.